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1. 发明申请

WO2002066972A3 METHODS AND MEANS FOR DETERMINING FITNESS IN PLANTS 审中-公开
公开(公告)号：WO2002066972A3
公开(公告)日：2002-08-29
申请号：PCT/EP2002/001991
申请日：2002-02-19
申请人： BAYER BIOSCIENCE N.V. , DE BLOCK, Marc
发明人： DE BLOCK, Marc
IPC分类号： G01N33/50
摘要： The present invention provides methods and means for determining parent inbred plant lines with good combining ability, for determining good combinations of parent inbred plant lines capable of yielding hybrid lines with high heterosis, and further for determining the agronomical performance of different plant lines, which can be performed in vitro by determining the electron flow in the mitochondria under control and stress conditions.

2. 发明申请

WO1997006267A2 GENETIC TRANSFORMATION USING A PARP INHIBITOR 审中-公开
标题翻译：使用PARP抑制剂进行遗传转化
公开(公告)号：WO1997006267A2
公开(公告)日：1997-02-20
申请号：PCT/EP1996003366
申请日：1996-07-31
申请人： PLANT GENETIC SYSTEMS, N.V. , DE BLOCK, Marc
发明人： PLANT GENETIC SYSTEMS, N.V.
IPC分类号： C12N15/82
CPC分类号： G01N33/5091 , C12N15/79 , C12N15/8201 , C12N15/8213 , C12N15/8289 , G01N33/5097
摘要： The invention concerns a process for producing transgenic eucaryotic cells, particularly plants, which comprises: contacting a culture of untransformed cells with an inhibitor of poly-(ADP-ribose) polymerase for a period of time sufficient to reduce the response of the cultured cells to stress and to reduce their metabolism. The untransformed cells are then contacted with foreign DNA comprising at least one gene of interest under conditions in which the foreign DNA is taken up by the untransformed cells and the gene of interest is stably integrated in the nuclear genome of the untransformed cells to produce the transgenic cells. Optionally, the transgenic cells are recovered from the culture. Preferably, the inhibitor is niacinamide, preferably at a concentration of about 200 mg/l to 500 mg/l and the untransformed cells are cultured in a medium containing the inhibitor for a period of time of approximately 3 to 14 days prior to the contacting with the foreign DNA. The invention also relates to a plant having in the nuclear genome of its cells foreign DNA integrated only in the regions of the nuclear genome that are transcriptionally active in cells of the plant when the cells are treated with an effective amount of a PARP inhibitor for a period of time sufficient to reduce cell metabolism to a state where gene expression is essentially limited to genes expressed irrespective of the differentiated or physiological condition of the cell.
摘要翻译：本发明涉及一种生产转基因真核细胞，特别是植物的方法，其包括：将未转化细胞的培养物与聚 - （ADP-核糖）聚合酶抑制剂接触一段时间，足以将培养细胞的反应降低至压力并减少其新陈代谢。然后将未转化的细胞与包含至少一种目的基因的外源DNA在外源DNA被未转化的细胞摄取的条件下接触，并且感兴趣的基因稳定地整合到未转化细胞的核基因组中以产生转基因细胞。任选地，从培养物中回收转基因细胞。优选地，抑制剂是烟酰胺，优选浓度为约200mg / l至500mg / l，并且将未转化细胞在含有抑制剂的培养基中培养约3至14天的时间之后，在与外来DNA。本发明还涉及一种在细胞的核基因组中具有外源DNA的植物，所述外源DNA仅在核基因组的区域中整合，当细胞用有效量的PARP抑制剂处理时，其在植物细胞中具有转录活性足以将细胞代谢降低到基因表达基本上限于基因表达而不管细胞的分化或生理状态表达的状态的时间段。

3. 发明申请

WO2011157453A1 METHODS FOR DETERMINING FITNESS IN PLANTS 审中-公开
标题翻译：用于确定植物适应性的方法
公开(公告)号：WO2011157453A1
公开(公告)日：2011-12-22
申请号：PCT/EP2011/003052
申请日：2011-06-15
申请人： BAYER BIOSCIENCE N.V. , AKPO, Essè, Ifêbi, Hervé , LAGA, Benjamin , DE BLOCK, Marc
发明人： AKPO, Essè, Ifêbi, Hervé , LAGA, Benjamin , DE BLOCK, Marc
IPC分类号： A01H5/00 , A01H1/04
CPC分类号： C12Q1/6895 , A01G22/00 , A01H1/04 , A01H5/00 , C12Q2600/154 , Y02A40/14
摘要： Means and methods are provided to produce abiotic stress tolerant plants with improved yield based on the specific identification of a DNA methylation signature in said plants out of a population of said plants.
摘要翻译：提供了基于在所述植物群体中所述植物中DNA甲基化特征的特异性鉴定来产生具有提高的产量的非生物胁迫耐受植物的方法和方法。

4. 发明申请

WO2009109315A2 MODULATION OF LIGNIN CONTENT AND COMPOSITION IN FEEDSTOCK CROP BIOMASS 审中-公开
标题翻译：饲料生物质中木质素含量和组成的调节
公开(公告)号：WO2009109315A2
公开(公告)日：2009-09-11
申请号：PCT/EP2009001302
申请日：2009-02-23
申请人： BAYER BIOSCIENCE NV , DE BLOCK MARC
发明人： DE BLOCK MARC
IPC分类号： C12N15/82
CPC分类号： C12N15/8246 , C12N15/8218 , C12N15/8242
摘要： Methods are provided to reduce the lignin content in plant cell biomass by expression of a heterologous N-acetylglucosamine transferase, such as chitin synthase or a NODC type protein in cells of the plant. Further provided are methods to countereffect potential negative effects caused by expression of the heterologous N-acetylglucosamine transferase.
摘要翻译：提供了通过在植物细胞中表达异源N-乙酰葡糖胺转移酶（例如几丁质合酶或NODC型蛋白）来减少植物细胞生物质中的木质素含量的方法。还提供了反映由异源N-乙酰葡糖胺转移酶的表达引起的潜在负面影响的方法。

5. 发明申请

WO2006136351A3 METHODS FOR ALTERING THE REACTIVITY OF PLANT CELL WALLS 审中-公开
标题翻译：改善植物细胞壁蛋白活性的方法
公开(公告)号：WO2006136351A3
公开(公告)日：2007-06-21
申请号：PCT/EP2006005853
申请日：2006-06-19
申请人： BAYER BIOSCIENCE NV , DE BLOCK MARC , MEULEWAETER FRANK , KOCH RAINHARD , ESSIGMANN BERND
发明人： DE BLOCK MARC , MEULEWAETER FRANK , KOCH RAINHARD , ESSIGMANN BERND
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10 , C12N5/04 , C12N5/10 , C12N15/54 , D06M101/06
CPC分类号： C12N15/8246 , C12N9/1051 , D02G3/02 , Y10T428/249921
摘要： Methods and means are provided for the modification of the reactivity of plant cell walls, particularly as they can be found in natural fibers of fiber producing plants by inclusion of positively charged oligosaccharides or polysaccharides into the cell wall. This can be conveniently achieved by expressing a chimeric gene encoding an N-acetylglucosamine transferase, particularly an N-acetylglucosamine transferase, capable of being targeted to the membranes of the Golgi apparatus in cells of a plant.
摘要翻译：提供了用于修饰植物细胞壁的反应性的方法和手段，特别是通过将带正电的寡糖或多糖包含在细胞壁中，可以在纤维生产植物的天然纤维中发现。这可以通过表达编码能够靶向植物细胞中高尔基体膜的N-乙酰葡糖胺转移酶，特别是N-乙酰葡糖胺转移酶的嵌合基因来方便地实现。

6. 发明申请

WO2006136351A2 METHODS FOR ALTERING THE REACTIVITY OF PLANT CELL WALLS 审中-公开
标题翻译：改善植物细胞壁蛋白活性的方法
公开(公告)号：WO2006136351A2
公开(公告)日：2006-12-28
申请号：PCT/EP2006/005853
申请日：2006-06-19
申请人： BAYER BIOSCIENCE N.V. , DE BLOCK, Marc , MEULEWAETER, Frank , KOCH, Rainhard , ESSIGMANN, Bernd
发明人： DE BLOCK, Marc , MEULEWAETER, Frank , KOCH, Rainhard , ESSIGMANN, Bernd
IPC分类号： C12N15/82 , C12N15/54 , C12N15/04 , C12N5/10 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8246 , C12N9/1051 , D02G3/02 , Y10T428/249921
摘要： Methods and means are provided for the modification of the reactivity of plant cell walls, particularly as they can be found in natural fibers of fiber producing plants by inclusion of positively charged oligosaccharides or polysaccharides into the cell wall. This can be conveniently achieved by expressing a chimeric gene encoding an N-acetylglucosamine transferase, particularly an N-acetylglucosamine transferase, capable of being targeted to the membranes of the Golgi apparatus in cells of a plant.
摘要翻译：提供了用于修饰植物细胞壁的反应性的方法和手段，特别是通过将带正电的寡糖或多糖包含在细胞壁中，可以在纤维生产植物的天然纤维中发现。这可以通过表达编码能够靶向植物细胞中高尔基体膜的N-乙酰葡糖胺转移酶，特别是N-乙酰葡糖胺转移酶的嵌合基因来方便地实现。

7. 发明申请

WO2006131222A1 INCREASE OF STRESS TOLERANCE BY APPLICATION OF NEONICOTINOIDS ON PLANTS ENGINEERED TO BE STRESS TOLERANT 审中-公开
标题翻译：通过施用抗氧化剂对植物施用耐受力的植物增加胁迫耐受性
公开(公告)号：WO2006131222A1
公开(公告)日：2006-12-14
申请号：PCT/EP2006/005072
申请日：2006-05-26
申请人： BAYER CROPSCIENCE AG , THIELERT, Wolfgang , METZLAFF, Michael , DE BLOCK, Marc
发明人： THIELERT, Wolfgang , METZLAFF, Michael , DE BLOCK, Marc
IPC分类号： A01N51/00 , A01N47/40 , A01N43/40
CPC分类号： A01N51/00 , A01N43/40 , A01N47/40
摘要： The present invention relates to methods for increasing the stress tolerance in plants and plant cells whereby neonicotinoid compounds, such as but not limited to imidacloprid, clothianidin, thiamethoxam, dinotefuran, nitenpyram, acetamiprid or thiacloprid, are applied to plants, or cells thereof, which comprise a genome that has been modified to make the plants or their cells more stress tolerant i.e. plants engineered to be stress tolerant. Particularly effective stress tolerance synergists in combination with genetically modified stress tolerant plants or their cells are neonicotinoid compounds which comprise a chloropyridine side chain, like e.g. imidacloprid, thiacloprid, acetamiprid, nitenpyram and 6-chloronicotinic acid (6-CNA).
摘要翻译：本发明涉及用于增加植物和植物细胞中的胁迫耐受性的方法，其中将新烟碱类化合物（例如但不限于吡虫啉，噻虫胺，噻虫嗪，二头孢呋喃，烯啶虫啶，啶虫脒或噻虫啉）施用于植物或细胞，其中包括已被修饰以使植物或其细胞更具有胁迫耐受性的基因组，即被工程化为胁迫耐受的植物。与转基因耐受植物或其细胞组合的特别有效的胁迫耐受增效剂是包含氯吡啶侧链的新烟碱类化合物，吡虫啉，噻虫啉，啶虫脒，烯啶虫胺和6-氯烟酸（6-CNA）。

8. 发明申请

WO2006032469A3 STRESS RESISTANT PLANTS 审中-公开
公开(公告)号：WO2006032469A3
公开(公告)日：2006-03-30
申请号：PCT/EP2005/010168
申请日：2005-09-16
申请人： BAYER BIOSCIENCE N.V. , DE BLOCK, Marc , METZLAFF, Michael , GOSSELE, Véronique
发明人： DE BLOCK, Marc , METZLAFF, Michael , GOSSELE, Véronique
IPC分类号： C12N15/82 , C12N9/80 , C12N9/10 , C12N9/12 , A01H5/00
摘要： Stress tolerance in plants and plant cells is achieved by using nucleotide sequences encoding enzymes involved in the NAD salvage synthesis pathway and/or the NAD de novo synthesis pathway e.g. for overexpression in plants.

9. 发明申请

WO2004090140A2 METHODS AND MEANS FOR INCREASING THE TOLERANCE OF PLANTS TO STRESS CONDITIONS 审中-公开
标题翻译：提高植物适应胁迫条件的方法和手段
公开(公告)号：WO2004090140A2
公开(公告)日：2004-10-21
申请号：PCT/EP2004/003995
申请日：2004-04-09
申请人： BAYER BIOSCIENCE N.V. , DE BLOCK, Marc
发明人： DE BLOCK, Marc
IPC分类号： C12N15/82
CPC分类号： C12Y302/01143 , C12N9/0028 , C12N9/2402 , C12N15/8271 , C12Y105/01011
摘要： Methods and means are provided to increase the tolerance of plants to abiotic stress or adverse growing conditions, including drought, high light intensities, high temperatures, nutrient limitations and the like by reducing the activity of endogenous PARG proteins in plants.
摘要翻译：提供了通过降低内源性PARG蛋白的活性来提高植物对非生物胁迫或不利生长条件包括干旱，高光强度，高温，营养限制等的耐受性的方法和手段在植物中。

10. 发明申请

WO1987005629A1 PLANT CELLS RESISTANT TO GLUTAMINE-SYNTHETASE INHIBITORS, MADE BY GENETIC ENGINEERING 审中-公开
标题翻译：植物细胞抗谷氨酰胺合成酶抑制剂，通过遗传工程制造
公开(公告)号：WO1987005629A1
公开(公告)日：1987-09-24
申请号：PCT/EP1987000141
申请日：1987-03-11
申请人： PLANT GENETIC SYSTEMS N.V. , BIOGEN N.V. , LEEMANS, Jan , ROTTERMAN, Johan , DE BLOCK, Marc , THOMPSON, Charles , MOUVA, Rao
发明人： PLANT GENETIC SYSTEMS N.V. , BIOGEN N.V.
IPC分类号： C12N15/00
CPC分类号： A01H1/00 , C07K2319/00 , C12N9/1029 , C12N15/63 , C12N15/70 , C12N15/76 , C12N15/8277 , Y10S47/01
摘要： DNA fragment containing a determined gene, the expression of which inhibits the antibiotic and herbicidal effects of Bialaphos and related products. It also relates to recombinant vectors, containing such DNA fragment, which enable this protective gene to be introduced and expressed into cells and plant cells.

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