专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2004108260A3 DEVICES AND METHODS FOR BIOMATERIAL PRODUCTION 审中-公开
标题翻译：用于生物体生产的装置和方法
公开(公告)号：WO2004108260A3
公开(公告)日：2005-05-12
申请号：PCT/US2004016806
申请日：2004-05-27
申请人： ADVISYS INC , HEBEL HENRY , RAMAKRISHNAN SRIRAM , GONZALEZ HUGO , DARNELL JEFF
发明人： HEBEL HENRY , RAMAKRISHNAN SRIRAM , GONZALEZ HUGO , DARNELL JEFF
IPC分类号： B01F13/02 , C12N1/06 , C12N15/10 , B01F3/04
CPC分类号： C12N15/1003 , B01F13/0255 , B03D2203/003 , C12M47/06 , C12M47/10 , C12N1/06 , C12N1/066 , C12N15/101
摘要： An apparatus and a method for isolating a biologic product, such as plasmid DNA, from cells. The method involves lysing cells in a controlled manner separate insoluble components from a fluid lysate containing cellular components of interest, followed by membrane chromatographic techniques to purify the cellular components of interest. The process utilizes a unique lysis apparatus, ion exchange and, optionally, hydrophobic interaction chromatography membranes in cartridge form, and ultrafiltration. The process can be applied to any biologic product extracted from a cellular source. The process uses a lysis apparatus, including a high shear, low residence-time mixer for advantageously mixing a cell suspension with a lysis solution, a hold time that denatures impurities, and an air-sparging bubble mixer that gently yet thoroughly mixes lysed cells with a neutralization/precipiation buffer and floats compacted precipitated cellular material.
摘要翻译：从细胞中分离生物制品如质粒DNA的装置和方法。该方法包括以受控的方式裂解细胞，从含有感兴趣的细胞成分的流体裂解物中分离出不溶性组分，然后进行膜色谱技术纯化感兴趣的细胞组分。该方法利用独特的裂解装置，离子交换以及任选的以胶囊形式的疏水相互作用层析膜和超滤。该方法可以应用于从细胞来源提取的任何生物制品。该方法使用裂解装置，包括高剪切，低停留时间混合器，有利地将细胞悬浮液与裂解溶液混合，使杂质变性的保持时间和将裂解细胞轻轻地充分混合的空气鼓泡混合器中和/沉淀缓冲液和漂浮压实的沉淀的细胞材料。

2. 发明申请

WO2005025669A3 CONSTANT CURRENT ELECTROPORATION DEVICE AND METHODS OF USE 审中-公开
公开(公告)号：WO2005025669A3
公开(公告)日：2005-03-24
申请号：PCT/US2004/028998
申请日：2004-09-03
申请人： ADVISYS, INC. , SMITH, Louis, C. , DRAGHIA-AKLI, Ruxandra , KHAN, Amir, S. , CARPENTER, Robert, H. , DARNELL, Jeff
发明人： SMITH, Louis, C. , DRAGHIA-AKLI, Ruxandra , KHAN, Amir, S. , CARPENTER, Robert, H. , DARNELL, Jeff
IPC分类号： A61N1/32
摘要： An electroporation device which may be used to effectively facilitate the introduction of a macromolecule into cells of a selected tissue in a body or plant. The electroporation device comprises an electro-kinetic device (" EKD") whose operation is specified by software or firmware. The EKD produces a series of programmable constant-current pulse patterns between electrodes in an array based on user control and input of the pulse parameters and allows the storage and acquisition of current waveform data. The electroporation device also comprises a replaceable electrode disk having an array of needle electrodes, a central injection channel for an injection needle, and a removable guide disk.

3. 发明申请

WO2004108260A2 DEVICES AND METHODS FOR BIOMATERIAL PRODUCTION 审中-公开
标题翻译：用于生物体生产的装置和方法
公开(公告)号：WO2004108260A2
公开(公告)日：2004-12-16
申请号：PCT/US2004/016806
申请日：2004-05-27
申请人： ADVISYS, INC. , HEBEL, Henry , RAMAKRISHNAN, Sriram , GONZALEZ, Hugo , DARNELL, Jeff
发明人： HEBEL, Henry , RAMAKRISHNAN, Sriram , GONZALEZ, Hugo , DARNELL, Jeff
IPC分类号： B01F3/04
CPC分类号： C12N15/1003 , B01F13/0255 , B03D2203/003 , C12M47/06 , C12M47/10 , C12N1/06 , C12N1/066 , C12N15/101
摘要： An apparatus and a method for isolating a biologic product, such as plasmid DNA, from cells. The method involves lysing cells in a controlled manner separate insoluble components from a fluid lysate containing cellular components of interest, followed by membrane chromatographic techniques to purify the cellular components of interest. The process utilizes a unique lysis apparatus, ion exchange and, optionally, hydrophobic interaction chromatography membranes in cartridge form, and ultrafiltration. The process can be applied to any biologic product extracted from a cellular source. The process uses a lysis apparatus, including a high shear, low residence-time mixer for advantageously mixing a cell suspension with a lysis solution, a hold time that denatures impurities, and an air-sparging bubble mixer that gently yet thoroughly mixes lysed cells with a neutralization/precipiation buffer and floats compacted precipitated cellular material.
摘要翻译：从细胞中分离生物制品如质粒DNA的装置和方法。该方法包括以受控的方式裂解细胞，从含有感兴趣的细胞成分的流体裂解物中分离出不溶性组分，然后进行膜色谱技术纯化感兴趣的细胞组分。该方法利用独特的裂解装置，离子交换以及任选的以胶囊形式的疏水相互作用层析膜和超滤。该方法可以应用于从细胞来源提取的任何生物制品。该方法使用裂解装置，包括高剪切，低停留时间混合器，有利地将细胞悬浮液与裂解溶液混合，使杂质变性的保持时间和将裂解细胞轻轻地充分混合的空气鼓泡混合器中和/沉淀缓冲液和漂浮压实的沉淀的细胞材料。

4. 发明申请

WO2005025669A2 CONSTANT CURRENT ELECTROPORATION DEVICE AND METHODS OF USE 审中-公开
标题翻译：恒定电流电镀装置和使用方法
公开(公告)号：WO2005025669A2
公开(公告)日：2005-03-24
申请号：PCT/US2004028998
申请日：2004-09-03
申请人： ADVISYS INC , SMITH LOUIS C , DRAGHIA-AKLI RUXANDRA , KHAN AMIR S , CARPENTER ROBERT H , DARNELL JEFF
发明人： SMITH LOUIS C , DRAGHIA-AKLI RUXANDRA , KHAN AMIR S , CARPENTER ROBERT H , DARNELL JEFF
IPC分类号： A61N1/30 , A61N1/32
CPC分类号： A61N1/0412 , A61N1/0476 , A61N1/327
摘要： An electroporation device which may be used to effectively facilitate the introduction of a macromolecule into cells of a selected tissue in a body or plant. The electroporation device comprises an electro-kinetic device (" EKD") whose operation is specified by software or firmware. The EKD produces a series of programmable constant-current pulse patterns between electrodes in an array based on user control and input of the pulse parameters and allows the storage and acquisition of current waveform data. The electroporation device also comprises a replaceable electrode disk having an array of needle electrodes, a central injection channel for an injection needle, and a removable guide disk.
摘要翻译：可用于有效促进将大分子引入身体或植物中选定组织的细胞中的电穿孔装置。电穿孔装置包括电动装置（“EKD”），其操作由软件或固件指定。基于用户控制和脉冲参数输入，EKD在阵列中的电极之间产生一系列可编程的恒定电流脉冲模式，并允许存储和采集电流波形数据。电穿孔装置还包括具有针电极阵列的可更换电极盘，用于注射针的中央注射通道以及可移除导向盘。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式