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2. 发明申请

WO2008109176A8 ASSAYS AND OTHER REACTIONS INVOLVING DROPLETS 审中-公开
标题翻译：测定和涉及滴剂的其他反应
公开(公告)号：WO2008109176A8
公开(公告)日：2008-11-13
申请号：PCT/US2008003185
申请日：2008-03-07
申请人： HARVARD COLLEGE , AGRESTI JEREMY , CHU LIANG-YIN , WEITZ DAVID A , KIM JIN-WOONG , ROWAT AMY , SOMMER MORTEN , DANTAS GAUTAM , CHURCH GEORGE
发明人： AGRESTI JEREMY , CHU LIANG-YIN , WEITZ DAVID A , KIM JIN-WOONG , ROWAT AMY , SOMMER MORTEN , DANTAS GAUTAM , CHURCH GEORGE
IPC分类号： C12Q1/68 , B01J13/00
CPC分类号： C12Q1/6848 , B01F3/0807 , B01F3/0811 , B01F13/0062 , B01F13/0071 , B01F2215/0037 , B01J13/0052 , B01J13/0065 , C12P19/34 , C12Q1/6834 , C12Q1/686
摘要： The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. In some aspects, a heterogeneous assay can be performed using a gel. For example, a droplet may be hardened to form a gel, where the droplet contains a cell, DNA, or other suitable species. The gel may be exposed to a reactant, and the reactant may interact with the gel and/or with the cell, DNA, etc., in some fashion. For example, the reactant may diffuse through the gel, or the hardened particle may liquefy to form a liquid state, allowing the reactant to interact with the cell. As a specific example, DNA contained within a gel particle may be subjected to PCR (polymerase chain reaction) amplification, e.g., by using PCR primers able to bind to the gel as it forms. As the DNA is amplified using PCR, some of the DNA will be bound to the gel via the PCR primer. After the PCR reaction, unbound DNA may be removed from the gel, e.g., via diffusion or washing. Thus, a gel particle having bound DNA may be formed in one embodiment of the invention.
摘要翻译：本发明一般涉及液滴和/或乳液，例如多重乳液。在一些情况下，液滴和/或乳液可以用于测定中，并且在某些实施方案中，液滴或乳液可以被硬化以形成凝胶。在一些方面，可以使用凝胶进行非均相测定。例如，可以使小滴硬化以形成凝胶，其中小滴含有细胞，DNA或其他合适的物质。凝胶可以暴露于反应物，并且反应物可以以某种方式与凝胶和/或与细胞，DNA等相互作用。例如，反应物可以通过凝胶扩散，或者硬化的颗粒可以液化以形成液体状态，从而允许反应物与细胞相互作用。作为具体例子，可以对凝胶颗粒内包含的DNA进行PCR（聚合酶链式反应）扩增，例如通过使用能够在凝胶形成时结合到凝胶上的PCR引物。当使用PCR扩增DNA时，一些DNA将通过PCR引物与凝胶结合。 PCR反应后，未结合的DNA可以从凝胶中去除，例如通过扩散或洗涤。因此，可以在本发明的一个实施方案中形成具有结合DNA的凝胶颗粒。

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