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1. 发明授权

US5582983A Genetic markers and methods of identifying alexandrium (dinophyceae) species 失效
标题翻译：鉴定亚历山大藻（dinophyceae）物种的遗传标记和方法
公开(公告)号：US5582983A
公开(公告)日：1996-12-10
申请号：US259745
申请日：1994-06-14
申请人： Donald M. Anderson , Christopher A. Scholin
发明人： Donald M. Anderson , Christopher A. Scholin
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6888 , C12Q1/6895
摘要： Nucleotide sequences from various species of Alexandrium are described. The sequences are used to construct nucleotide probes for assaying samples to determine the presence of selected species of Alexandrium.
摘要翻译：描述了来自各种亚历山大藻的核苷酸序列。序列用于构建用于测定样品的核苷酸探针，以确定所选择的亚历山大藻种类的存在。

2. 发明授权

US06187530B1 Aquatic autosampler device 有权
标题翻译：水上自动进样器装置
公开(公告)号：US06187530B1
公开(公告)日：2001-02-13
申请号：US09319333
申请日：1999-07-15
申请人： Christopher A. Scholin , Eugene I. Massion , David K. Wright , Danelle E. Cline , Ed Mellinger , Mark Brown
发明人： Christopher A. Scholin , Eugene I. Massion , David K. Wright , Danelle E. Cline , Ed Mellinger , Mark Brown
IPC分类号： C12Q100
CPC分类号： G01N1/405 , G01N1/14 , G01N1/16 , G01N1/4077 , G01N33/18 , G01N33/1866 , Y10S436/807
摘要： An aquatic autosampler device for frequent collection of discrete samples of microorganisms at various locations and depths. The aquatic autosampler device moves a filter disk within a filter housing from a filter carousel into a filter housing holder. The filter housing holder, slidably mounted on a linear shuttle, moves the filter housing into a process position, where a syringe draws fluid in through a valve manifold and through the filter disk to collect samples on the filter disk. Reagents may then be passed over the filter disk to enable image-based identification and quantification of the cells on the filter disk. An imaging system on the autosampler may capture a fluorescent image of the microorganisms on the filter disk and store the image in a control computer. The filter housing may then be returned to the filter carousel for storage and further lab processing.
摘要翻译：一种用于在不同位置和深度频繁收集微生物的离散样本的水生自动进样器装置。水上自动进样器装置将过滤器壳体内的过滤盘从过滤器转盘移动到过滤器壳体保持器中。可滑动地安装在线性梭上的过滤器壳体保持器将过滤器壳体移动到处理位置，其中注射器通过阀歧管抽吸流体并通过过滤盘以将样品收集在过滤盘上。然后可以将试剂通过过滤盘，以使得基于图像的识别和量化过滤器盘上的单元。自动进样器上的成像系统可以捕获过滤盘上的微生物的荧光图像，并将图像存储在控制计算机中。然后可以将过滤器壳体返回到过滤器转盘以进行存储和进一步的实验室处理。

3. 发明授权

US5958689A Detection of toxigenic marine diatoms of the genus Pseudo-nitzschia 失效
标题翻译：检测假硝酸菌的产毒海洋硅藻
公开(公告)号：US5958689A
公开(公告)日：1999-09-28
申请号：US861096
申请日：1997-05-21
申请人： Christopher A. Scholin , Gerard A. Cangelosi , Paul V. Haydock
发明人： Christopher A. Scholin , Gerard A. Cangelosi , Paul V. Haydock
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04 , C12N15/00
CPC分类号： C12Q1/6888 , C12Q1/6895
摘要： The present invention provides compositions, methods, and kits for detecting species of Pseudo-nitzschia from a marine sample. Oligonucleotide probes for rRNA hypervariable regions of the Psuedo-nitzschia species: P. australis, P. pungens, P. multiseries, P. pseudodelicatissima, P. heimii, P. fraudulenta, P. delicatissima, and P. americana are provided as well as a oligonucleotide probe for a conserved region of ribosomal RNA from Pseudo-nitzschia.
摘要翻译：本发明提供了用于从海洋样品中检测伪硝化物种的组合物，方法和试剂盒。寡核苷酸探针用于拟南芥种类的rRNA高变区：P. alis属，P. pungens，P. multiseries，P. pseudodelicatissima，P. heimii，P. fraudulenta，P. delicatissima和P. americana，以及用于来自伪硝唑的核糖体RNA的保守区的寡核苷酸探针。

4. 发明授权

US06787648B2 Compositions and methods for detecting raphidophytes 失效
公开(公告)号：US06787648B2
公开(公告)日：2004-09-07
申请号：US09780113
申请日：2001-02-09
申请人： John V. Tyrrell , Patricia R. Bergquist , Peter L. Bergquist , Christopher A. Scholin
发明人： John V. Tyrrell , Patricia R. Bergquist , Peter L. Bergquist , Christopher A. Scholin
IPC分类号： C07H2102
CPC分类号： C07H21/04 , C12Q1/6895
摘要： Effective management of finfish stocks to avoid or mitigate the threat of fish-killing phytoplankton is of increasing concern, particularly in temperate seas. Intensive spatial and temporal sampling is required to monitor and quantify potentially harmful species, so that prior warning can be received of an imminent bloom. The use of large-subunit rRNA (LSU rRNA)-targeted oligonucleotide probes based on the sandwich hybridization assay to detect the fragile species Heterosigma akashiwo (Hada) Hada and Fibrocapsa japonica Toriumi & Takano (Raphidophyceae) is disclosed. Species-specific sandwich hybridization assays were successfully developed for various Raphidophytes.

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