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1. 发明授权

US07427472B2 Methods for the differentiation and identification of medically important endemic fungi 有权
标题翻译：分离和鉴定医学上重要的地方性真菌的方法
公开(公告)号：US07427472B2
公开(公告)日：2008-09-23
申请号：US10490726
申请日：2002-09-25
申请人： Mark D. Lindsley , Zhenyu Qin , Christine J. Morrison , Jong S. Choi
发明人： Mark D. Lindsley , Zhenyu Qin , Christine J. Morrison , Jong S. Choi
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C12Q1/6895
摘要： Methods of detecting a dimorphic fungus, including differentiating a dimorphic fungus from other fungi are disclosed. A sample suspected of containing a nucleic acid of a fungus, such as an internal transcribed spacer-2 (ITS2) nucleic acid sequence of a dimorphic fungal rDNA, is screened for the presence or absence of that nucleic acid. The presence of the nucleic acid indicates the sample was contacted by the fungus. Determining whether the nucleic acid sequence is present in the sample can be accomplished by detecting hybridization between a dimorphic probe, species-specific probe, and/or microbe-specific probe and a nucleic acid sequence corresponding to the ITS2 region of fungal rDNA. Kits and arrays for carrying out these methods also are disclosed.
摘要翻译：公开了检测二倍体真菌的方法，包括将二型真菌与其他真菌分离。筛选怀疑含有真菌核酸的样品，例如二型真菌rDNA的内部转录间隔子-2（ITS2）核酸序列，存在或不存在该核酸。核酸的存在表明样品被真菌接触。确定样品中核酸序列是否存在可以通过检测二型探针，物种特异性探针和/或微生物特异性探针与对应于真菌rDNA的ITS2区域的核酸序列之间的杂交来完成。也公开了用于实施这些方法的套件和阵列。

2. 发明授权

US07052836B2 Nucleic acids for detecting fusarium species 有权
标题翻译：用于检测镰孢属物种的核酸
公开(公告)号：US07052836B2
公开(公告)日：2006-05-30
申请号：US10046955
申请日：2002-01-14
申请人： Christine J. Morrison , Errol Reiss , Jong Soo Choi , Liliana Aidorevich
发明人： Christine J. Morrison , Errol Reiss , Jong Soo Choi , Liliana Aidorevich
IPC分类号： C07H21/04 , C12Q1/68
CPC分类号： C12Q1/6895
摘要： Nucleic acids for detecting Aspergillus species and other filamentous fungi are provided. Unique internal transcribed space 2 coding regions permit the development of nucleic acid probes specific for five different species of Aspergillus, three species of Fusarium, four species of Mucor, two species of Penecillium, five species of Rhizopus, one species of Rhizomucor, as well as probes for Absidia corymbifer, Cunninghamella elagans, Pseudallescheria boydii, and Sporothrix schenkii. Methods are disclosed for the species-specific detection and diagnosis of infection by Aspergillus, Fusarium, Mucor, Penecillium, Rhizomucor, absidia, Cunninghaemella, Pseudallescheria or Sporthrix in a subject. Furthermore, genus-specific probes are also provided for Aspergillus, Fusarium and Mucor, in addition to an all-fungus nucleic acid probe.
摘要翻译：提供了用于检测曲霉菌种和其他丝状真菌的核酸。独特的内部转录空间2编码区域允许开发针对五种不同种类的曲霉属，三种镰刀菌，四种毛霉属，两种Penecillium，五种根霉属，一种Rhizomucor的核酸探针，以及探针为茜草，葫芦科，Pseudallescheria boydii和Sporothrix schenkii。公开了用于物种特异性检测和诊断受试者中曲霉属，镰刀霉属，毛霉属，Penecillium，Rhizomucor，absidia，Cunninghaemella，Pseudallescheria或Sporthrix的感染的方法。此外，除了全真菌核酸探针之外，还提供了针对曲霉属，镰孢属（Fusarium）和毛霉属（Mucor）的属特异性探针。

3. 发明授权

US06235890B1 Methods and compositions for the detection of Candida spp. 有权
标题翻译：用于检测假丝酵母属的方法和组合物
公开(公告)号：US06235890B1
公开(公告)日：2001-05-22
申请号：US09269136
申请日：1999-07-12
申请人： Christine J. Morrison , Errol Reiss , Brian Holloway , Jong Hee Shin
发明人： Christine J. Morrison , Errol Reiss , Brian Holloway , Jong Hee Shin
IPC分类号： C07H2104
CPC分类号： C12Q1/6895
摘要： The present invention provides a rapid method for identifying species of Candida. Identification is through the use of a non-conserved regions of the ITS2 region flanked by highly conserved functional domains. Detection of members of the Candida and Aspergillus genera is enhanced by the detection of genus-specific regions of the 5.8S rRNA gene. The present invention provides isolated nucleic acids that selectively hybridize to the fungal genus-specific 5.8S rRNA region, and to the species-specific ITS2 region. The invention provides for nucleic acid sequences for use as selective probes for fungal genus-specific probes and as Candida species-specific probes. The present invention provides methods for the use of the Candida species-specific probes that allow the detection and identification of the individual species in biological samples.
摘要翻译：本发明提供了鉴定假丝酵母菌种的快速方法。鉴定是通过使用侧翼为高度保守的功能结构域的ITS2区域的非保守区域。通过检测5.8S rRNA基因的属特异性区域，可以增强念珠菌和曲霉属成员的检测。本发明提供了与真菌属特异性5.8S rRNA区域以及物种特异性ITS2区域选择性杂交的分离的核酸。本发明提供用作真菌属特异性探针和假丝酵母属特异性探针的选择性探针的核酸序列。本发明提供了使用允许在生物样品中检测和鉴定个体物种的假丝酵母属物种特异性探针的方法。

4. 发明授权

US5645992A Nucleic acid sequences and methods for detecting candida tropicalis in blood 失效
标题翻译：核酸序列和检测血液中念珠菌的方法
公开(公告)号：US5645992A
公开(公告)日：1997-07-08
申请号：US429522
申请日：1995-04-26
申请人： Timothy J. Lott , Christine J. Morrison , Errol Reiss , Brent Lasker , Sandra Zakroff
发明人： Timothy J. Lott , Christine J. Morrison , Errol Reiss , Brent Lasker , Sandra Zakroff
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6895 , C12Q1/6806
摘要： Provided is an isolated double-stranded nucleic acid consisting essentially of the nucleotide sequences defined in the Sequence Listing by SEQ ID Nos: 5-9. These are the ITS2 sequences for C. albicans, C. parapsilosis, C. tropicalis, C. glabrata and C. krusei. A method of diagnosing systemic candidiasis in a subject is also provided. The method comprises the steps of: (a) collecting blood from the subject into tubes containing detergent, polypropylene glycol, sodium poyantholesulfonate, and sodium ethylene diamine tetraacetic acid; (b) lysing Candida cells using ZYMOLYASE.RTM.-100T with agitation; (c) extracting and precipitating the DNA from the lysed cells; (d) amplifying the precipitated DNA using universal fungal primer pairs derived from the internal transcribed spacer regions of the Candida ribosomal DNA; and (e) detecting amplified DNA from Candida by hybridizing the amplified DNA with a probe that selectively hybridizes with Candida DNA, the presence of amplified DNA indicating systemic candidiasis.
摘要翻译：提供了基本上由序列表中由SEQ ID No：5-9定义的核苷酸序列组成的分离的双链核酸。这些是白色念珠菌，副伤寒杆菌，热带假丝酵母，光滑念珠菌和克氏梭菌的ITS2序列。还提供了诊断受试者体内念珠菌病的方法。该方法包括以下步骤：（a）将血液从受试者中收集到含有洗涤剂，聚丙二醇，聚苯磺酸钠和乙二胺四乙酸钠的管中; （b）在搅拌下使用ZYMOLYASE TM -100T裂解念珠菌细胞; （c）从裂解的细胞中提取和沉淀DNA; （d）使用衍生自假丝酵母核糖体DNA的内部转录间隔区的通用真菌引物对扩增沉淀的DNA; 和（e）通过将扩增的DNA与选择性地与念珠菌DNA杂交的探针杂交，检测来自假丝酵母的扩增的DNA，存在显示系统性念珠菌病的扩增的DNA。

5. 发明授权

US5426027A Nucleic acid probes and methods for detecting Candida DNA cells in blood 失效
标题翻译：核酸探针和检测血液中念珠菌DNA细胞的方法
公开(公告)号：US5426027A
公开(公告)日：1995-06-20
申请号：US65845
申请日：1993-05-20
申请人： Timothy J. Lott , Christine J. Morrison , Errol Reiss , Brent Lasker , Sandra Zakroff
发明人： Timothy J. Lott , Christine J. Morrison , Errol Reiss , Brent Lasker , Sandra Zakroff
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6895 , C12Q1/6806
摘要： Provided is an isolated double-stranded nucleic acid consisting essentially of the nucleotide sequence defined in the Sequence Listing by SEQ ID NO:5. This is the C. albicans ITS2 sequence and includes a nucleic acid comprising a nucleotide sequence that is specific for Candida albicans. Further examples of an isolated double stranded nucleic acid of the present invention consist essentially of the nucleotide sequences defined in the Sequence Listing by SEQ ID NOs:6-9. These are the ITS2 sequences for C. parapsilosis, C. tropicalis, C. glabrata and C. krusei. A method of diagnosing systemic candidiasis in a subject is also provided. The method comprises the steps of: (a) collecting blood from the subject into tubes containing detergent, polypropylene glycol, sodium polyanetholesulfonate, and sodium ethylene diamine.RTM. tetraacetic acid; (b) lysing Candida cells using ZYMOLYASE.RTM.-100T with agitation; (c) extracting and precipitating the DNA from the lysed cells; (d) amplifying the precipitated DNA using universal fungal primer pairs derived from the internal transcribed spacer regions of the Candida ribosomal DNA; and (e) detecting amplified DNA from Candida by hybridizing the amplified DNA with a probe that selectively hybridizes with Candida DNA, the presence of amplified DNA indicating systemic candidiasis.
摘要翻译：提供了基本上由SEQ ID NO：5的序列表中定义的核苷酸序列组成的分离的双链核酸。这是白色念珠菌ITS2序列，并且包括包含对白色念珠菌特异性的核苷酸序列的核酸。本发明的分离双链核酸的其它实例基本上由序列表SEQ ID NO：6-9中定义的核苷酸序列组成。这些是C.parapsilosis，C. tropicalis，C. glabrata和C.krusei的ITS2序列。还提供了诊断受试者体内念珠菌病的方法。该方法包括以下步骤：（a）将来自受试者的血液从含有洗涤剂，聚丙二醇，聚苯醚磺酸钠和乙二胺四乙酸钠的管中收集; （b）在搅拌下使用ZYMOLYASE TM -100T裂解念珠菌细胞; （c）从裂解的细胞中提取和沉淀DNA; （d）使用衍生自假丝酵母核糖体DNA的内部转录间隔区的通用真菌引物对扩增沉淀的DNA; 和（e）通过将扩增的DNA与选择性地与念珠菌DNA杂交的探针杂交，检测来自假丝酵母的扩增的DNA，存在显示系统性念珠菌病的扩增的DNA。

6. 发明授权

US08119788B2 Compositions and methods for the detection of Candida species 有权
标题翻译：用于检测念珠菌物种的组合物和方法
公开(公告)号：US08119788B2
公开(公告)日：2012-02-21
申请号：US12088235
申请日：2006-09-26
申请人： Christine J. Morrison , Sanchita Das , Teresa Brown , Brian Holloway
发明人： Christine J. Morrison , Sanchita Das , Teresa Brown , Brian Holloway
IPC分类号： C40B40/06
CPC分类号： C12Q1/6895
摘要： Compositions and methods for detecting and/or differentiating among Candida organisms, including C. albicans and C. dubliniensis, are disclosed. Exemplary methods involve screening a sample suspected of containing at least one or more Candida sp. for the presence or absence of a nucleic acid sequence specific for each such fungal pathogen. Some disclosed methods permit the rapid and simultaneous detection and identification of several fungal pathogens (e.g., up to 100 fungi) in a single sample.
摘要翻译：公开了用于检测和/或区分假丝酵母属生物体的组合物和方法，包括白色念珠菌和C.都柏林。示例性方法包括筛选怀疑含有至少一种或多种念珠菌属的样品。对于每种这样的真菌病原体存在或不存在特异性的核酸序列。一些公开的方法允许在单个样品中快速和同时检测和鉴定几种真菌病原体（例如多达100个真菌）。

7. 发明授权

US06372430B1 Nucleic acids for detecting Aspergillus species and other filamentous fungi 有权
标题翻译：用于检测曲霉菌和其他丝状真菌的核酸
公开(公告)号：US06372430B1
公开(公告)日：2002-04-16
申请号：US09423233
申请日：2000-06-27
申请人： Christine J. Morrison , Errol Reiss , Liliana Aidorevich , Jong Soo Choi
发明人： Christine J. Morrison , Errol Reiss , Liliana Aidorevich , Jong Soo Choi
IPC分类号： C12Q168
CPC分类号： C12Q1/6895
摘要： Nucleic acids for detecting Aspergillus species and other filamentous fungi are provided. Unique internal transcribed space 2 coding regions permit the development of nucleic acid probes specific for five different species of Aspergillus, three species of Fusarium, four species of Mucor, two species of Penecillium, five species of Rhizopus, one species of Rhizomucor, as well as probes for Absidia corymbifer, Cunninghamella elagans, Pseudallescheria boydii, and Sporothrix schenkii. Methods are disclosed for the species-specific detection and diagnosis of infection by Aspergillus, Fusarium, Mucor, Penecillium, Rhizomucor, absidia, Cunninghaemella, Pseudallescheria or Sporthrix in a subject. Furthermore, genus-specific probes are also provided for Aspergillus, Fusarium and Mucor, in addition to an all-fungus nucleic acid probe.
摘要翻译：提供了用于检测曲霉菌种和其他丝状真菌的核酸。独特的内部转录空间2编码区域允许开发针对五种不同种类的曲霉属，三种镰刀菌，四种毛霉属，两种Penecillium，五种根霉属，一种Rhizomucor的核酸探针，以及探针为茜草，葫芦科，Pseudallescheria boydii和Sporothrix schenkii。公开了用于物种特异性检测和诊断受试者中曲霉属，镰刀霉属，毛霉属，Penecillium，Rhizomucor，absidia，Cunninghaemella，Pseudallescheria或Sporthrix的感染的方法。此外，除了全真菌核酸探针之外，还提供了针对曲霉属，镰孢属（Fusarium）和毛霉属（Mucor）的属特异性探针。

8. 发明授权

US06242178B1 Nucleic acid probes for detecting Candida species 失效
标题翻译：核酸探针用于检测念珠菌属
公开(公告)号：US06242178B1
公开(公告)日：2001-06-05
申请号：US08903446
申请日：1997-07-30
申请人： Timothy J. Lott , Cheryl M. Elie , Christine J. Morrison , Errol Reiss
发明人： Timothy J. Lott , Cheryl M. Elie , Christine J. Morrison , Errol Reiss
IPC分类号： C12Q168
CPC分类号： C12Q1/6895
摘要： The nucleic acid sequence encoding the internal transcribed spacer 2 region of Candida, the organism causing candidiasis, for various Candida species. Nucleic acid molecules useful as probes for detecting Candida species are described. The nucleic acid molecules are useful in methods for the detection and diagnosis of Candida infection in a sample or subject.
摘要翻译：编码念珠菌内部转录间隔区2区域的核酸序列，引起念珠菌病的生物，用于各种假丝酵母属物种。描述了可用作检测假丝酵母属物种的探针的核酸分子。核酸分子可用于检测和诊断样品或受试者中念珠菌感染的方法。

9. 发明授权

US5688644A Nucleic acid probes for candida parapsilosis and methods for detecting candidiasis in blood 失效
标题翻译：用于念珠菌的核酸探针和用于检测血液中念珠菌病的方法
公开(公告)号：US5688644A
公开(公告)日：1997-11-18
申请号：US429520
申请日：1995-04-26
申请人： Timothy J. Lott , Christine J. Morrison , Errol Reiss , Brent Lasker , Sandra Zakroff
发明人： Timothy J. Lott , Christine J. Morrison , Errol Reiss , Brent Lasker , Sandra Zakroff
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6895 , C12Q1/6806
摘要： Provided is an isolated double-stranded nucleic acid consisting essentially of the nucleotide sequences defined in the Sequence Listing by SEQ ID Nos:5-9. These are the ITS2 sequences for C. albicans, C. parapsilosis, C. tropicalis, C. glabrata and C. krusei. A method of diagnosing systemic candidiasis in a subject is also provided. The method comprises the steps of: (a) collecting blood from the subject into tubes containing detergent, polypropylene glycol, sodium poyantholesulfonate, and sodium ethylene diamine tetraacetic acid; (b) lysing Candida cells using ZYMOLYASE.RTM.-100T.TM. enzyme mixture with agitation; (c) extracting and precipitating the DNA from the lysed cells; (d) amplifying the precipitated DNA using universal fungal primer pairs derived from the internal transcribed spacer regions of the Candida ribosomal DNA; and (e) detecting amplified DNA from Candida by hybridizing the amplified DNA with a probe that selectively hybridizes with Candida DNA, the presence of amplified DNA indicating systemic candidiasis.
摘要翻译：提供了基本上由序列表中由SEQ ID No：5-9定义的核苷酸序列组成的分离的双链核酸。这些是白色念珠菌，副伤寒杆菌，热带假丝酵母，光滑念珠菌和克氏梭菌的ITS2序列。还提供了诊断受试者体内念珠菌病的方法。该方法包括以下步骤：（a）将血液从受试者中收集到含有洗涤剂，聚丙二醇，聚苯磺酸钠和乙二胺四乙酸钠的管中; （b）搅拌使用ZYMOLYASE TM-1100T TM酶混合物裂解念珠菌细胞; （c）从裂解的细胞中提取和沉淀DNA; （d）使用衍生自假丝酵母核糖体DNA的内部转录间隔区的通用真菌引物对扩增沉淀的DNA; 和（e）通过将扩增的DNA与选择性地与念珠菌DNA杂交的探针杂交，检测来自假丝酵母的扩增的DNA，存在显示系统性念珠菌病的扩增的DNA。

10. 发明授权

US07871779B2 Molecular identification of Aspergillus species 有权
标题翻译：曲霉菌的分子鉴定
公开(公告)号：US07871779B2
公开(公告)日：2011-01-18
申请号：US12115493
申请日：2008-05-05
申请人： Christine J. Morrison , Hans Peter Hinrikson
发明人： Christine J. Morrison , Hans Peter Hinrikson
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C12Q1/6895
摘要： Novel techniques for the detection of Aspergillus in samples are disclosed. These techniques relate to PCR amplification and/or detection of Aspergillus ITS1 rDNA sequences, and the identification of particular species of Aspergillus by detecting differences in the ITS1-V1, ITS-V2, ITS-V3, ITS-V4, and ITS-V5 nucleic acid sequences of Aspergillus. The highly variable regions of the ITS1 rDNA sequences are particularly useful in distinguishing, for example, Aspergillus clavatus, Aspergillus granulosus, Aspergillus sydowii, Aspergillus flavipes, Aspergillus restrictus, Aspergillus versicolor, Aspergillus wentii, and Aspergillus chevalieri. In particular embodiments, the sequence differences are also able to distinguish among variants of particular species, such as Aspergillus granulosus CBS 119.5A, Aspergillus granulosus strain NRRL 1932, Aspergillus sydowii strain NRRL 250, Aspergillus sydowii strain NRRL 4768, Aspergillus sydowii strain CUHI, Aspergillus sydowii strain CUH2, Aspergillus sydowii strain CUH7, and Aspergillus sydowii strain CUH8.
摘要翻译：公开了用于检测样品中曲霉菌的新技术。这些技术涉及通过检测ITS1-V1，ITS-V2，ITS-V3，ITS-V4和ITS-V5核酸中的差异来鉴定曲霉ITS1 rDNA序列的PCR扩增和/或检测，并鉴定特定的曲霉种曲霉酸序列。 ITS1 rDNA序列的高度可变区特别可用于区分例如曲霉菌（Aspergillus clavatus），粒细菌（Aspergillus granulosus），曲霉（Aspergillus sydowii），曲霉（Aspergillus flavipes），限制曲霉（Aspergillus restrictionus），曲霉（Aspergillus versicolor），曲霉（Aspergillus goii）和曲霉（Aspergillus chevalieri）。在特定的实施方案中，序列差异也能够区分特定种类的变体，例如粒细菌芽孢杆菌CBS 119.5A，粒细菌菌株NRRL 1932，曲霉菌株NRRL 250，曲霉菌菌株NRRL 4768，曲霉菌菌株CUHI，曲霉属（Aspergillus sydowii） sydowii菌株CUH2，曲霉菌菌株CUH7和曲霉菌菌株CUH8。

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