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1. 发明授权

US5256557A Purified alkaline protease concentrate and method of preparation 失效
标题翻译：纯化碱性蛋白酶浓缩物及其制备方法
公开(公告)号：US5256557A
公开(公告)日：1993-10-26
申请号：US813705
申请日：1991-12-27
申请人： Jayarama K. Shetty , Chimanbhai P. Patel , Mary A. Nicholson
发明人： Jayarama K. Shetty , Chimanbhai P. Patel , Mary A. Nicholson
IPC分类号： C11D3/00 , C11D3/386 , C12N9/54 , C12N9/56 , C12R1/07 , C12R1/10
CPC分类号： C11D3/0078 , C11D3/386 , C12N9/54 , Y10S435/816
摘要： A highly purified alkaline protease preparation is produced by separating a mixture containing amorphous form and crystalline form of alkaline protease from ultrafiltrate containing impurities, through a method which includes the incubation of an aqueous alkaline protease concentrate with sodium chloride and carbohydrate hydrolyse enzymes at an elevated temperature greater than 20.degree. C. and a pH between pH 4.0 and pH 6.5 at constant agitation.

2. 发明授权

US5439817A Method of preparation of purified alkaline protease 失效
标题翻译：纯碱性蛋白酶的制备方法
公开(公告)号：US5439817A
公开(公告)日：1995-08-08
申请号：US006484
申请日：1993-01-21
申请人： Jayarama K. Shetty , Chimanbhai P. Patel , Mary Ann Nicholson
发明人： Jayarama K. Shetty , Chimanbhai P. Patel , Mary Ann Nicholson
IPC分类号： C11D3/00 , C11D3/386 , C12N9/54 , C12N9/56 , C12R1/07 , C12R1/10
CPC分类号： C11D3/0078 , C11D3/386 , C12N9/54 , Y10S435/816
摘要： A highly purified alkaline protease preparation is produced by separating a mixture containing amorphous form and crystalline form of alkaline protease from ultrafiltrate containing impurities, through a method which includes the incubation of an aqueous alkaline protease concentrate with sodium chloride and carbohydrate hydrolyse enzymes at an elevated temperature greater than 20.degree. C. and a pH between pH 4.0 and pH 6.5 at constant agitation.
摘要翻译：通过包括将碱性蛋白酶水溶液与氯化钠和碳水化合物水解酶在升高的温度下孵育的方法，通过从包含杂质的超滤液中分离含有无定形形式和结晶形式的碱性蛋白酶的混合物来制备高度纯化的碱性蛋白酶制剂大于20℃，pH在pH4.0和pH6.5之间。

3. 发明授权

US5405767A Purified enzyme concentrate and method of preparation 失效
标题翻译：纯化酶浓缩物及其制备方法
公开(公告)号：US5405767A
公开(公告)日：1995-04-11
申请号：US865252
申请日：1992-04-08
申请人： Jayarama K. Shetty , Chimanbhai P. Patel
发明人： Jayarama K. Shetty , Chimanbhai P. Patel
IPC分类号： C11D7/42 , C11D3/00 , C11D3/386 , C12N9/00 , C12N9/54 , C12N9/56 , C12R1/07 , C12R1/10 , G02C13/00 , C12N9/14 , C12N9/24
CPC分类号： C11D3/386 , C11D3/0078 , C12N9/00 , C12N9/54 , Y10S435/816
摘要： A novel compound for producing a purified enzyme precipitate and a method of preparing the purified enzyme precipitate, wherein the compound is an organic compound selected from the group consisting of carboxylic acids having at least 2 carboxyl groups, salts or esters of these carboxylic acids, amino acids, salts or esters of these amino acids.
摘要翻译：用于生产纯化酶沉淀物的新型化合物和制备纯化酶沉淀物的方法，其中所述化合物是选自具有至少2个羧基的羧酸，这些羧酸的盐或酯，氨基这些氨基酸的酸，盐或酯。

4. 发明授权

US4931397A Method for removing antifoaming agents during processing of microbial fermentations 失效
标题翻译：在微生物发酵加工过程中除去消泡剂的方法
公开(公告)号：US4931397A
公开(公告)日：1990-06-05
申请号：US779504
申请日：1985-09-24
申请人： Curtis J. Montgomery , Chimanbhai P. Patel , Jayarama K. Shetty
发明人： Curtis J. Montgomery , Chimanbhai P. Patel , Jayarama K. Shetty
IPC分类号： C12N1/00 , C12N9/00 , C12N9/28 , C12N9/54 , C12P1/00
CPC分类号： C12N9/2417 , C12N9/00 , C12N9/54 , C12P1/00 , Y10S435/812 , Y10S435/814
摘要： Antifoaming agents are commonly used during culturing of enzyme-producing microorganisms. These antifoams often persist through enzyme processing, slowing filtrations, clogging filtration membranes and adversely affecting the quality of the final product. The present invention describes a method for removing antifoams, and often carbohydrates and pigments, from enzyme systems using mineral clay, the preferred clay being bentonite.
摘要翻译：消化剂通常用于培养产酶微生物。这些消泡剂通常通过酶加工持续，减缓过滤，堵塞过滤膜并且不利地影响最终产品的质量。本发明描述了一种从使用矿物粘土的酶体系中去除消泡剂，常常是碳水化合物和颜料的方法，优选的粘土是膨润土。

5. 发明授权

US4717665A Recovery of microbial lipase 失效
标题翻译：微生物脂肪酶的回收
公开(公告)号：US4717665A
公开(公告)日：1988-01-05
申请号：US863716
申请日：1986-05-16
申请人： Robert L. Charles , Steven C. Dolby , Chimanbhai P. Patel , Jayarama K. Shetty
发明人： Robert L. Charles , Steven C. Dolby , Chimanbhai P. Patel , Jayarama K. Shetty
IPC分类号： C12N9/00 , C12N9/20 , C12R1/685 , C12R1/72 , C12R1/785 , C12R1/845
CPC分类号： C12N9/20 , C12N9/00 , Y10S435/814
摘要： Disclosed is a method for causing the dissociation of microbial mycelium and extracellular lipase bound thereto and increasing the measurable activity of the lipase. The method involves treating an aqueous suspension of the mycelium with the anhydride of a dicarboxylic acid which results in dissociation of the mycelium and lipase thereby facilitating recovery of the lipase.
摘要翻译：公开了一种使微生物菌丝体和细胞外脂肪酶结合的解离并增加脂肪酶的可测量活性的方法。该方法包括用二羧酸的酸酐处理菌丝体的水性悬浮液，导致菌丝体和脂肪酶解离，从而促进脂肪酶的回收。

6. 发明授权

US5759984A Enzyme stabilization 失效
标题翻译：酶稳定
公开(公告)号：US5759984A
公开(公告)日：1998-06-02
申请号：US724253
申请日：1996-09-30
申请人： Jayarama K. Shetty , Chimanbhai P. Patel
发明人： Jayarama K. Shetty , Chimanbhai P. Patel
IPC分类号： C11D3/386 , C12N9/64 , C12N9/96
CPC分类号： C12N9/6408 , C12N9/96
摘要： Methods for stabilizing enzymes in liquid compositions, including those liquid compositions having a high water content and those stabilized enzyme liquid compositions formed thereby. The method involves forming the enzyme, so that it is in an insoluble form thereof and then adding thereto an agent for maintaining the enzyme in the insoluble form thereof. Examples of such insoluble forms are crystal forms of the enzyme. Enzymes which are stabilized in this manner are useful for combining with liquid compositions, including liquid compositions having a high water content. The method is particularly useful for the preparation of stable enzyme-containing liquid detergent compositions.
摘要翻译：用于稳定液体组合物中酶的方法，包括那些具有高含水量的液体组合物和由此形成的那些稳定的酶液体组合物。该方法包括形成酶，使其为不溶形式，然后向其中加入用于将酶维持在其不溶形式中的试剂。这种不溶形式的实例是酶的晶体形式。以这种方式稳定的酶可用于与液体组合物组合，包括具有高水含量的液体组合物。该方法对于制备稳定的含酶液体洗涤剂组合物特别有用。

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