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1. 发明申请

WO2008117176A2 COMPOSITION 审中-公开
标题翻译：组成
公开(公告)号：WO2008117176A2
公开(公告)日：2008-10-02
申请号：PCT/IB2008/001351
申请日：2008-03-12
申请人： DANISCO A/S , HAUGEN, Kersti , WISLER, Peter , VEDEL, Charlotte, Johansen
发明人： HAUGEN, Kersti , WISLER, Peter , VEDEL, Charlotte, Johansen
CPC分类号： C09D5/1625
摘要： There is provided a paint composition comprising i) a surface coating material; ii) a pigment; and iii) natamycin.
摘要翻译：提供了一种涂料组合物，其包含：i）表面涂层材料; ii）颜料; 和iii）那他霉素。

2. 发明申请

WO2011045629A1 LIPID ACYLTRANSFERASE PROTEINS AND METHODS OF MAKING THEM 审中-公开
标题翻译：脂质过氧化物酶蛋白及其制备方法
公开(公告)号：WO2011045629A1
公开(公告)日：2011-04-21
申请号：PCT/IB2009/054535
申请日：2009-10-15
申请人： DANISCO A/S , BRUNSTEDT, Janne , SØRENSEN, Jens, Frisbæk , SØE, Jørn, Borch , VEDEL, Charlotte, Johansen , WITTSCHIEBEN, Birgitte, Ø. , MIKKELSEN, Jørn, Dalgaard , POULSEN, Charlotte, Horsmans , JENSEN, Lene, B. , MILLER, Lone, B. , LARSEN, Morten, K , LORENTZEN, Rikke, H. , THOUDAHL, Charlotte, R. , CERVIN, Marguerite, A. , BOTT, Richard, R.
发明人： BRUNSTEDT, Janne , SØRENSEN, Jens, Frisbæk , SØE, Jørn, Borch , VEDEL, Charlotte, Johansen , WITTSCHIEBEN, Birgitte, Ø. , MIKKELSEN, Jørn, Dalgaard , POULSEN, Charlotte, Horsmans , JENSEN, Lene, B. , MILLER, Lone, B. , LARSEN, Morten, K , LORENTZEN, Rikke, H. , THOUDAHL, Charlotte, R. , CERVIN, Marguerite, A. , BOTT, Richard, R.
IPC分类号： C12N9/18 , C12N15/75 , C12N1/21
CPC分类号： C12N9/1029 , A21D8/02 , A23V2002/00 , C12N15/00
摘要： In one aspect of the present invention there is provided a method for preparing a variant lipid acyltransferase enzyme comprising expressing in a host organism a nucleotide sequence which has at least 90% identity with a nucleotide sequence encoding a parent lipid acyltransferase and comprises at least one modification (suitably at least two modifications) at a position(s) which corresponds in the encoded amino acid sequence to an amino acid(s) located in a) the canyon region of the enzyme (i.e. preferably amino acid residues 31, 27, 85, 86, 119, and 120); and/or b) insertion site 1 (i.e. amino acid residues 22-36) and/or c) insertion site 2 (i.e. amino acid residues 74-88), wherein the canyon region, insertion site 1 and/or insertion site 2 are defined as that region which when aligned based on primary or tertiary structure corresponds to the canyon region, insertion site 1 or insertion site 2 (or the corresponding amino acid residues taught above) of the enzyme shown herein as SEQ ID No. 16 or 6. Preferable modifications are one or more of the following L3 IQ, H, N, T, F, Y or C (preferably L31 Q); M27R, G, H, K, Y, D, N, V, C, Q, L, E, S or F (preferably M27V); V85H, R5 D or E; I86R,Y, S, V, I, As T, M, F, C or L (preferably I86S or A); Al 19T or I; Y120K or E; W122S, L or A (preferably W122L); E201R; Q245S; F235A or V; W232G or S; and/or A236G or E.
摘要翻译：在本发明的一个方面，提供了一种制备变体脂质酰基转移酶的方法，包括在宿主生物体中表达与编码亲本脂质酰基转移酶的核苷酸序列具有至少90％同一性的核苷酸序列，并且包含至少一个修饰（适当地至少两个修饰）在编码的氨基酸序列中对应的位置与位于a）谷氨酸区域的氨基酸（即，优选氨基酸残基31,27,85， 86,119和120）; 和/或b）插入位点1（即氨基酸残基22-36）和/或c）插入位点2（即氨基酸残基74-88），其中峡谷区，插入位点1和/或插入位点2是定义为当基于一级或三级结构对准的区域对应于如本文所示的酶的SEQ ID No.16或6的峡谷区，插入位点1或插入位点2（或上文所述的相应的氨基酸残基）。优选的修饰是以下L3 IQ，H，N，T，F，Y或C（优选L31Q）中的一种或多种; M27R，G，H，K，Y，D，N，V，C，Q，L，E，S或F（优选M27V）; V85H，R5 D或E; I86R，Y，S，V，I，As T，M，F，C或L（优选I86S或A）; Al 19T或I; Y120K或E; W122S，L或A（优选W122L）; E201R; Q245S; F235A或V; W232G或S; 和/或A236G或E.

3. 发明申请

WO2008117176A3 COMPOSITION 审中-公开
标题翻译：组成
公开(公告)号：WO2008117176A3
公开(公告)日：2008-11-20
申请号：PCT/IB2008001351
申请日：2008-03-12
申请人： DANISCO , HAUGEN KERSTI , WISLER PETER , VEDEL CHARLOTTE JOHANSEN
发明人： HAUGEN KERSTI , WISLER PETER , VEDEL CHARLOTTE JOHANSEN
IPC分类号： C09D5/02
CPC分类号： C09D5/1625
摘要： There is provided a paint composition comprising i) a surface coating material; ii) a pigment; and iii) natamycin.
摘要翻译：提供了一种涂料组合物，其包含i）表面涂层材料; ii）颜料; 和iii）那他霉素。

4. 发明授权

US09175271B2 Lipid acyltransferase proteins and methods of making them 有权
标题翻译：脂质酰基转移酶蛋白及其制备方法
公开(公告)号：US09175271B2
公开(公告)日：2015-11-03
申请号：US13444302
申请日：2012-04-11
申请人： Janne Brunstedt , Jens Frisbæk Sorensen , Jørn Borch Søe , Charlotte Johansen Vedel , Birgitte Ø. Wittschieben , Jørn Dalgaard Mikkelsen , Charlotte Horsmans Poulsen , Lene B. Jensen , Lone B. Miller , Morten K. Larsen , Rikke H. Lorentzen , Charlotte R. Thoudahl , Marguerite A. Cervin , Richard R. Bott
发明人： Janne Brunstedt , Jens Frisbæk Sorensen , Jørn Borch Søe , Charlotte Johansen Vedel , Birgitte Ø. Wittschieben , Jørn Dalgaard Mikkelsen , Charlotte Horsmans Poulsen , Lene B. Jensen , Lone B. Miller , Morten K. Larsen , Rikke H. Lorentzen , Charlotte R. Thoudahl , Marguerite A. Cervin , Richard R. Bott
IPC分类号： C12N9/10 , C12N15/00 , C07H21/04 , C12N1/20 , A21D8/02 , A21D10/00
CPC分类号： C12N9/1029 , A21D8/02 , A23V2002/00 , C12N15/00
摘要： The present invention provides a method for preparing a variant lipid acyltransferase enzyme by expressing a nucleotide sequence encoding a lipid acyltransferase which may comprise at least one modification at a position(s) which corresponds in the encoded amino acid sequence to an amino acid(s) located in a) the canyon region of the enzyme (i.e. preferably amino acid residues 31, 27, 85, 86, 119, and 120); and/or b) insertion site 1 (i.e. amino acid residues 22-36) and/or c) insertion site 2 (i.e. amino acid residues 74-88), wherein the canyon region, insertion site 1 and/or insertion site 2 are defined as that region which when aligned based on primary or tertiary structure corresponds to the canyon region, insertion site 1 or insertion site 2 (or the corresponding amino acid residues taught above) of the enzyme shown herein as SEQ ID No. 16 or 6 in a host organism.
摘要翻译：本发明提供了一种通过表达编码脂质酰基转移酶的核苷酸序列来制备变体脂质酰基转移酶的方法，所述脂质酰基转移酶可以在编码的氨基酸序列中相应于氨基酸的位置包含至少一个修饰，位于a）谷的峡谷区域（即优选氨基酸残基31,27,85,86,119和120）; 和/或b）插入位点1（即氨基酸残基22-36）和/或c）插入位点2（即氨基酸残基74-88），其中峡谷区域，插入位点1和/或插入位点2是定义为当基于一级或三级结构对准的区域对应于本文所示的酶的SEQ ID No.16或6的峡谷区域，插入位点1或插入位点2（或上文所述的相应氨基酸残基）宿主生物。

5. 发明申请

US20130034627A1 LIPID ACYLTRANSFERASE PROTEINS AND METHODS OF MAKING THEM 有权
标题翻译：脂质过氧化物酶蛋白及其制备方法
公开(公告)号：US20130034627A1
公开(公告)日：2013-02-07
申请号：US13444302
申请日：2012-04-11
申请人： Janne Brunstedt , Jens Frisbæk Sorensen , Jørn Borch Søe , Charlotte Johansen Vedel , Birgitte Ø. Wittschieben , Jørn Dalgaard Mikkelsen , Charlotte Horsmans Poulsen , Lene B. Jensen , Lone B. Miller , Morten K. Larsen , Rikke H. Lorentzen , Charlotte R. Thoudahl , Marguerite A. Cervin , Richard R. Bott
发明人： Janne Brunstedt , Jens Frisbæk Sorensen , Jørn Borch Søe , Charlotte Johansen Vedel , Birgitte Ø. Wittschieben , Jørn Dalgaard Mikkelsen , Charlotte Horsmans Poulsen , Lene B. Jensen , Lone B. Miller , Morten K. Larsen , Rikke H. Lorentzen , Charlotte R. Thoudahl , Marguerite A. Cervin , Richard R. Bott
IPC分类号： C12N9/10 , A21D13/00 , A23D9/04 , C12N15/54 , A21D8/04
CPC分类号： C12N9/1029 , A21D8/02 , A23V2002/00 , C12N15/00
摘要： The present invention provides a method for preparing a variant lipid acyltransferase enzyme by expressing a nucleotide sequence encoding a lipid acyltransferase which may comprise at least one modification at a position(s) which corresponds in the encoded amino acid sequence to an amino acid(s) located in a) the canyon region of the enzyme (i.e. preferably amino acid residues 31, 27, 85, 86, 119, and 120); and/or b) insertion site 1 (i.e. amino acid residues 22-36) and/or c) insertion site 2 (i.e. amino acid residues 74-88), wherein the canyon region, insertion site 1 and/or insertion site 2 are defined as that region which when aligned based on primary or tertiary structure corresponds to the canyon region, insertion site 1 or insertion site 2 (or the corresponding amino acid residues taught above) of the enzyme shown herein as SEQ ID No. 16 or 6 in a host organism.
摘要翻译：本发明提供了一种通过表达编码脂质酰基转移酶的核苷酸序列来制备变体脂质酰基转移酶的方法，所述脂质酰基转移酶可以在编码的氨基酸序列中相应于氨基酸的位置包含至少一个修饰，位于a）谷的峡谷区域（即优选氨基酸残基31,27,85,86,119和120）; 和/或b）插入位点1（即氨基酸残基22-36）和/或c）插入位点2（即氨基酸残基74-88），其中峡谷区域，插入位点1和/或插入位点2是定义为当基于一级或三级结构对准的区域对应于本文所示的酶的SEQ ID No.16或6的峡谷区域，插入位点1或插入位点2（或上文所述的相应氨基酸残基）宿主生物。

6. 发明授权

US07063970B1 Enzymatic preservation of water based paints 失效
标题翻译：水性油漆的酶保存
公开(公告)号：US07063970B1
公开(公告)日：2006-06-20
申请号：US09564935
申请日：2000-05-04
申请人： Charlotte Johansen , Claus Crone Fuglsang
发明人： Charlotte Johansen , Claus Crone Fuglsang
IPC分类号： C12N9/00
CPC分类号： A01N59/00 , A01N63/02 , A01N63/04 , C09D5/14 , C12N9/0061 , C12N9/0065
摘要： A preserved and/or conserved water based paint composition comprising an oxidoreductase, an oxidizing agent, a binder and at least 10% w/w water.
摘要翻译：包含氧化还原酶，氧化剂，粘合剂和至少10％w / w水的保存和/或保存的水基涂料组合物。

7. 发明授权

US06794350B2 Reduction of malodor from laundry 失效
标题翻译：从衣物中减少恶臭
公开(公告)号：US06794350B2
公开(公告)日：2004-09-21
申请号：US10076231
申请日：2002-02-14
申请人： Charlotte Johansen , Signe Munk
发明人： Charlotte Johansen , Signe Munk
IPC分类号： C12S900
CPC分类号： C11D11/0017 , C11D3/0068 , C11D3/38636
摘要： The present invention relates to methods of reducing malodor in laundry, comprising contacting laundry with an enzyme having lysostaphin activity; and compositions comprising a surfactant and an enzyme having lysostaphin activity.
摘要翻译：本发明涉及减少衣物中恶臭的方法，包括将衣物与具有溶葡萄球菌素活性的酶接触; 以及包含表面活性剂和具有溶葡萄球菌素活性的酶的组合物。

8. 发明授权

US06592867B2 Antimicrobial composition containing an oxidoreductase and an enhancer of the N-hydroxyanilide-type 失效
标题翻译：含有氧化还原酶的抗微生物组合物和N-羟基苯胺类型的增强剂
公开(公告)号：US06592867B2
公开(公告)日：2003-07-15
申请号：US09437106
申请日：1999-11-09
申请人： Charlotte Johansen , Heinz-Josef Deussen
发明人： Charlotte Johansen , Heinz-Josef Deussen
IPC分类号： A61K3844
CPC分类号： C11D3/38654 , A61K8/42 , A61K8/463 , A61K8/69 , A61K8/97 , A61K8/99 , A61K2800/524 , A61Q17/005 , C09D5/025 , C11D3/32 , C11D3/33 , C11D3/48
摘要： The present invention relates to an enzymatic composition capable of killing or inhibiting microbial cells or micro-organisms, e.g. in laundry, on hard surfaces, in water systems, on skin, on teeth or on mucous membranes. The present invention also relates to the use of said enzymatic composition for preserving food products, cosmetics, paints, coatings, etc.
摘要翻译：本发明涉及能够杀死或抑制微生物细胞或微生物的酶组合物，例如，在洗衣，硬表面，水系统，皮肤上，牙齿上或粘膜上。本发明还涉及所述酶组合物用于保存食品，化妆品，油漆，涂料等的用途。

9. 发明授权

US06323007B1 2,6-&bgr;-D-fructan hydrolase enzyme and processes for using the enzyme 失效
标题翻译： 2,6-β-D-果聚糖水解酶和使用该酶的方法
公开(公告)号：US06323007B1
公开(公告)日：2001-11-27
申请号：US09397885
申请日：1999-09-17
申请人： Soren Moller , Charlotte Johansen , Thomas Schafer , Peter Rahbek Ostergaard , Lisbeth Hedegaard Hoeck
发明人： Soren Moller , Charlotte Johansen , Thomas Schafer , Peter Rahbek Ostergaard , Lisbeth Hedegaard Hoeck
IPC分类号： C12P1944
CPC分类号： C12P19/14 , A61K8/64 , A61K8/66 , A61K2800/70 , A61K2800/86 , A61Q11/00 , A61Q17/005 , C11D3/386 , C12N9/2431 , C12Y302/01065
摘要： The present invention relates to isolated polypeptides having polypeptide having 2,6-&bgr;-D-fructan hydrolase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
摘要翻译：本发明涉及具有2,6-β-D-果聚糖水解酶活性的多肽的分离多肽和编码该多肽的分离的核酸序列。本发明还涉及包含核酸序列的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。

10. 发明授权

US06287585B1 Methods for laundry using polycations and enzymes 失效
标题翻译：使用聚阳离子和酶的洗衣方法
公开(公告)号：US06287585B1
公开(公告)日：2001-09-11
申请号：US09143622
申请日：1998-08-28
申请人： Charlotte Johansen
发明人： Charlotte Johansen
IPC分类号： A01N2500
CPC分类号： C11D3/48 , A01N25/10 , A01N33/04 , A01N33/08 , A01N37/12 , A01N37/20 , A01N63/00 , C11D3/3719 , C11D3/3769 , C11D3/38636 , C11D3/38654 , A01N2300/00
摘要： The present invention provides a method of killing or inhibiting the growth of microbial cells present on laundry, comprising contacting the cells with a composition comprising a poly-cationic compound, preferably a polyamino acid, a polyvinylamine, a copolymer prepared from vinylamine and one or more carboxylic acid anhydrides, e.g. a polymer comprising 0.1-100 mol % vinyl amine or ethyleneimine units, 0-99.9 mol % units of at least one monomer selected from N-vinylcarboxamides of the formula I wherein R1 and R2 are hydrogen or C1-C6-alkyl; vinyl formate, vinyl acetate, vinyl propionate, vinyl alcohol, C1-C6-alkyl vinyl ether, mono ethylenic unsaturated C3-C8-carboxylic acid, and esters, nitrites, amides and anhydrides thereof, N-vinylurea, N-imidazoles and N-vinyl imidazolines; and 0-5 mol % units of monomers having at least two unsaturated ethylenic double bonds; and one or more enzymes, preferably glycanases, muranases, oxidoreductases, glucanases, proteases, amylases, lipases, pectinases and xylanases.
摘要翻译：本发明提供了杀死或抑制存在于衣物上的微生物细胞生长的方法，包括使细胞与包含多阳离子化合物，优选聚氨基酸的聚合物，聚乙烯胺，由乙烯胺制备的共聚物和一种或多种羧酸酐，例如包含0.1-100mol％乙烯基胺或乙烯亚胺单元的聚合物，0-99.9mol％单元的至少一种选自式I的N-乙烯基甲酰胺的单体，其中R 1和R 2是氢或C 1 -C 6烷基;甲酸乙烯酯，乙烯基乙酸乙烯酯，丙酸乙烯酯，乙烯醇，C1-C6烷基乙烯基醚，单烯属不饱和C3-C8-羧酸及其酯，亚硝酸酯，酰胺和酸酐，N-乙烯基脲，N-咪唑和N-乙烯基咪唑; 和至少两个不饱和烯键式双键的单体为0.5〜5摩尔％;以及一种或多种酶，优选聚糖酶，杂交酶，氧化还原酶，葡聚糖酶，蛋白酶，淀粉酶，脂肪酶，果胶酶和木聚糖酶。

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