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    • 2. 发明授权
    • Method for making a biochip and biochip
    • 制造生物芯片和生物芯片的方法
    • US06803228B1
    • 2004-10-12
    • US09868043
    • 2001-06-14
    • Patrice CaillatCharles Rosilio
    • Patrice CaillatCharles Rosilio
    • C12Q168
    • G01N33/5438B01J19/0046B01J2219/00317B01J2219/00653B01J2219/00659B01J2219/00713B01J2219/00722C12Q1/6837C40B40/06C40B60/14
    • The present invention relates to a method to produce a biochip and to a biochip, said biochip being composed particularly of biological probes grafted onto a conductive polymer. The method according to the invention comprises the following steps: a) structuring of a substrate so as to obtain on said substrate microtroughs comprising in their base a layer of a material capable of initiating and promoting the adhesion onto said layer of a film of a pyrrole and functionalised pyrrole copolymer by electropolymerisation, b) collective electropolymerisation, so as to form an electropolymerised film of a pyrrole and functionalised pyrrole copolymer on the base of said microtroughs, c) direct or indirect fixation of functionalised oligonucleotides by microdeposition or a liquid jet printing technique.
    • 本发明涉及一种生产生物芯片和生物芯片的方法,所述生物芯片特别由接枝在导电聚合物上的生物探针组成。本发明的方法包括以下步骤:a)将基底结构化成 在所述基材微通道上获得能够通过电聚合引发和促进在所述吡咯和官能化吡咯共聚物的膜上的粘合层的材料层,b)集体电聚合,以便形成电聚合膜 在所述微通道的基底上的吡咯和官能化的吡咯共聚物,c)通过微沉积或液体喷射印刷技术直接或间接固定官能化的寡核苷酸。