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1. 发明申请

WO2017064629A1 CONFOCAL MICROSCOPY APPARATUS AND RELATED PROCESS FOR ACQUIRING AND PROCESSING IMAGES 审中-公开
标题翻译：共焦显微设备以及用于获取和处理图像的相关过程
公开(公告)号：WO2017064629A1
公开(公告)日：2017-04-20
申请号：PCT/IB2016/056100
申请日：2016-10-12
申请人： CRESTOPTICS S.R.L.
发明人： RICCO, Vincenzo , SANTINELLI, Andrea
IPC分类号： G02B21/00
CPC分类号： G02B21/0032 , G02B21/0044
摘要： Confocal microscopy apparatus (100; 200), and related process, comprising: a structured light generating component (2; 13) configured to be illuminated with a basic light beam and to generate a structured light beam focused on a first plane (3a); a spinning disk (7) configured to receive said structured light beam and to transmit a resulting excitation beam to an optics (8, 9) of a microscope (15) focused on a plane of a sample (10), wherein the spinning disk (7) lies on a second plane (3b) and comprises a disk-shaped substrate (490) composed of an optically transparent material, the substrate (490) of the spinning disk (7) comprising a planar first surface (456) and an opposing planar second surface (460) and a patterned mask (492) disposed on one of the first surface (456) and the second surface (460) and comprising at least one sector provided with one or more continuous spiral slit apertures (476), wherein the patterned mask (492) or an outer surface thereof is composed of a highly black material opaque to light; a housing configured to house on a third plane (3c) an acquisition sensor (11) configured to detect a fluorescent beam emitted from said plane of the sample (10); a set of relay lenses (4a, 4b, 4c) configured to optically conjugate the first plane (3a), the second plane (3b) and said plane of the sample (10) to the third plane (3c); optical means (5, 6) configured to transmit said structured light beam from the structured light generating component (2; 13) to said plane of the sample (10) and said emitted fluorescent beam from said plane of the sample (10) to said housing configured to house the acquisition sensor (11); and moving means configured to move the structured light generating component (2; 13), so as to shift the structured light beam in the first plane (3a), and the spinning disk (7) in the second plane (3b).
摘要翻译：共焦显微设备（100; 200）及相关方法，包括：结构化光产生组件（2; 13），其被配置为用基本光束照射并产生聚焦的结构化光束在第一平面（3a）上; 旋转盘（7），所述旋转盘构造成接收所述结构化光束并将得到的激励光束传输到聚焦在样品（10）的平面上的显微镜（15）的光学器件（8,9），其中所述旋转盘 7）位于第二平面（3b）上并且包括由光学透明材料构成的盘形衬底（490），所述旋转盘（7）的衬底（490）包括平坦的第一表面（456）和相对的第二表面（460）和设置在所述第一表面（456）和所述第二表面（460）中的一个表面上的图案化掩模（492），并且包括至少一个设置有一个或多个连续螺旋狭缝孔（476）的扇区，其中图案化掩模（492）或其外表面由对光不透明的高度黑色材料构成; 被配置为在第三平面（3c）上容纳被配置为检测从所述样本（10）的所述平面发射的荧光束的获取传感器（11）的外壳; （3a），第二平面（3b）和样品（10）的所述平面光学共轭到第三平面（3c）的一组中继透镜（4a，4b，4c）; 配置成将来自结构化光产生组件（2; 13）的所述结构化光束透射到样品（10）的所述平面并且将所述发射的荧光束从样品（10）的所述平面透射到所述光学装置被配置为容纳所述采集传感器（11）的外壳; 以及移动装置，其被配置为移动结构化光产生组件（2; 13），以便在第一平面（3a）中移动结构化光束，并且在第二平面（3b）中移动旋转盘（7） p>

2. 发明申请

WO2016199025A1 CONFOCAL MICROSCOPE AND RELATED PROCESS OF ACQUIRING AND PROCESSING IMAGES 审中-公开
标题翻译：共焦显微镜及其获取和处理图像的相关过程
公开(公告)号：WO2016199025A1
公开(公告)日：2016-12-15
申请号：PCT/IB2016/053344
申请日：2016-06-08
申请人： CRESTOPTICS S.R.L. , CONSIGLIO NAZIONALE DELLE RICERCHE
发明人： VITULANO, Domenico , BRUNI, Vittoria , RICCO, Vincenzo , SANTINELLI, Andrea
IPC分类号： G02B21/00 , G02B27/58
CPC分类号： G02B21/008 , G02B21/0044 , G02B21/0072 , G02B27/58
摘要： Process of acquiring and processing images in confocal microscopy, comprising the following steps: A. acquiring (500, 510; 520, 530) a first overall image A k-1 of a plane of a sample (160) scanning the same with a first excitation light beam, obtained by making a basic light beam pass through first pinholes (1255; 1255') and transmitted to an optics (155) of a confocal microscope (100) that is focused on the plane of the sample (160), and acquiring a second overall image Ak of the plane of the sample (160) scanning the same with a second excitation light beam, obtained by making said basic light beam pass through second pinholes (1265; 1265') having same shape and size (L 2 ; L a2 , L b2 ) larger than the first pinholes (1255; 1255'); B. calculating (540) an estimate g of the PSF of the optics (155) of the microscope (100), through an inverse Fourier transform of formula (I): where Â k -1 (ω) is the Fourier transform of the acquired first overall image A k-1 and Â k (ω) is the Fourier transform of the acquired second overall image A k ; and C. deconvolving (550) the acquired second overall image A k using the estimate g of the PSF of the optics (155) of the microscope (100) through a main deconvolution algorithm, obtaining the resulting image / of the entire plane of the sample (160): I = A k • g.
摘要翻译：在共聚焦显微镜中获取和处理图像的过程包括以下步骤：A.采集（500,510,520,530）第一个整体图像Ak-1，其中第一个整体图像用第一个激发扫描的样品（160）的平面通过使基本光束通过第一针孔（1255; 1255'）并被传输到聚焦在样品（160）的平面上的共聚焦显微镜（100）的光学器件（155）而获得的光束，并获取通过使所述基本光束通过具有相同形状和尺寸（L2; La2）的第二针孔（1265; 1265'）而获得的样品（160）的平面的第二整体图像Ak与第二激发光束扫描相同，Lb2）大于第一针孔（1255; 1255'）; B.通过公式（I）的傅立叶逆变换计算（540）显微镜（100）的光学器件（155）的PSF的估计值：其中kk（ω）是所获取的傅立叶变换第一整体图像Ak-1和k（ω）是所获取的第二整体图像Ak的傅立叶变换; 和C.通过主去卷积算法使用显微镜（100）的光学器件（155）的PSF的估计值g来获取的第二整体图像Ak解卷积（550），获得样品的整个平面的所得图像（160）：I = Ak·g。

3. 发明公开

EP3308211A1 CONFOCAL MICROSCOPE AND RELATED PROCESS OF ACQUIRING AND PROCESSING IMAGES 有权转让
标题翻译：共焦显微镜以及获取和处理图像的相关过程
公开(公告)号：EP3308211A1
公开(公告)日：2018-04-18
申请号：EP16751335.7
申请日：2016-06-08
申请人： Crestoptics S.r.l. , Consiglio Nazionale Delle Ricerche
发明人： VITULANO, Domenico , BRUNI, Vittoria , RICCO, Vincenzo , SANTINELLI, Andrea
IPC分类号： G02B21/00 , G02B27/58
CPC分类号： G02B21/008 , G02B21/0044 , G02B21/0072 , G02B27/58
摘要： Process of acquiring and processing images in confocal microscopy, comprising the following steps: A. acquiring (500, 510; 520, 530) a first overall image Ak-1 of a plane of a sample (160) scanning the same with a first excitation light beam, obtained by making a basic light beam pass through first pinholes (1255; 1255') and transmitted to an optics (155) of a confocal microscope (100) that is focused on the plane of the sample (160), and acquiring a second overall image Ak of the plane of the sample (160) scanning the same with a second excitation light beam, obtained by making said basic light beam pass through second pinholes (1265; 1265') having same shape and size (L2; La2, Lb2) larger than the first pinholes (1255; 1255'); B. calculating (540) an estimate g of the PSF of the optics (155) of the microscope (100), through an inverse Fourier transform of formula (I): where Âk -1(ω) is the Fourier transform of the acquired first overall image Ak-1 and Âk(ω)is the Fourier transform of the acquired second overall image Ak; and C. deconvolving (550) the acquired second overall image Ak using the estimate g of the PSF of the optics (155) of the microscope (100) through a main deconvolution algorithm, obtaining the resulting image / of the entire plane of the sample (160): I = Ak• g.

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