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1. 发明申请

WO2011035422A1 METHOD OF PREPARING PLANT-DERIVED VLPS 审中-公开
标题翻译：制备植物衍生VLPS的方法
公开(公告)号：WO2011035422A1
公开(公告)日：2011-03-31
申请号：PCT/CA2010/001488
申请日：2010-09-21
申请人： MEDICAGO INC. , VEZINA, Louis-Philippe , COUTURE, Manon , PAQUET, Dany , DARGIS, Michele , D'AOUST, Marc-Andre
发明人： VEZINA, Louis-Philippe , COUTURE, Manon , PAQUET, Dany , DARGIS, Michele , D'AOUST, Marc-Andre
IPC分类号： C12N7/02 , A61K39/12 , A61K39/145 , A61P31/16 , A61P37/04 , C12N7/00
CPC分类号： A61K39/145 , A61K39/12 , A61K2039/517 , A61K2039/5258 , C12N7/00 , C12N15/8203 , C12N15/8257 , C12N15/8258 , C12N2760/16123 , C12N2760/16134
摘要： Methods of preparing plant-derived virus like particles (VLPs) are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized VLPs, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived VLPs. Alternatively, VLPs may be obtained from plant or plant matter comprising plant-derived VLPs by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant- derived VLPs are recovered from the filtered fraction.
摘要翻译：提供了植物来源的病毒样颗粒（VLPs）的制备方法。该方法可以包括获得植物或包含原生质体定位的VLP的植物物质，从植物或植物物质产生原生质体/原生质体级分和原生质体级分，以及回收载脂蛋白级分。载脂蛋白级分包含植物来源的VLP。或者，VLP可以通过使用细胞壁降解酶组合物消化植物物质从包含植物来源的VLP的植物或植物物质获得，以产生消化部分。将消化的馏分过滤以产生过滤的馏分，并从过滤的馏分中回收来自植物的VLP。

2. 发明申请

WO2010148511A8 CHIMERIC INFLUENZA VIRUS-LIKE PARTICLES COMPRISING HEMAGGLUTININ 审中-公开
标题翻译：含有HEMAGGLUTININ的CHIMERIC INFLUENZA病毒颗粒
公开(公告)号：WO2010148511A8
公开(公告)日：2011-03-10
申请号：PCT/CA2010000983
申请日：2010-06-25
申请人： MEDICAGO INC , COUTURE MANON , DARGIS MICHELE , LAVOIE PIERRE-OLIVIER , VEZINA LOUIS-PHILIPPE , D AOUST MARC-ANDRE
发明人： COUTURE MANON , DARGIS MICHELE , LAVOIE PIERRE-OLIVIER , VEZINA LOUIS-PHILIPPE , D AOUST MARC-ANDRE
IPC分类号： C07K14/11 , A01H5/00 , A61K39/145 , A61P31/16 , C07K19/00 , C12N7/00 , C12N7/01 , C12N15/44 , C12N15/82
CPC分类号： A61K39/145 , A61K39/12 , A61K2039/5258 , C07K14/005 , C07K2319/00 , C07K2319/02 , C12N15/8257 , C12N2760/16122 , C12N2760/16123 , C12N2760/16134 , C12N2760/16222 , C12N2760/16223 , C12N2760/16234
摘要： A method for synthesizing chimeric influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of chimeric influenza HA in a plant or a portion of a plant. The invention is also directed towards a VLP comprising chimeric influenza HA protein and plants lipids. The invention is also directed to a nucleic acid encoding chimeric influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.
摘要翻译：提供了一种在植物或植物的一部分内合成嵌合型流感病毒样颗粒（VLP）的方法。该方法包括在植物或植物的一部分中表达嵌合流感HA。本发明还涉及包含嵌合流感HA蛋白和植物脂质的VLP。本发明还涉及编码嵌合流感HA以及载体的核酸。 VLP可用于制定流感疫苗，或可用于丰富现有疫苗。

3. 发明申请

WO2012171104A1 RABIES VIRUS LIKE PARTICLE PRODUCTION IN PLANTS 审中-公开
标题翻译： RABIES病毒样植物生产
公开(公告)号：WO2012171104A1
公开(公告)日：2012-12-20
申请号：PCT/CA2012/000581
申请日：2012-06-13
申请人： MEDICAGO INC. , D'AOUST, Marc-Andre , LAVOIE, Pierre-Olivier , VEZINA, Louis-Philippe , COUTURE, Manon
发明人： D'AOUST, Marc-Andre , LAVOIE, Pierre-Olivier , VEZINA, Louis-Philippe , COUTURE, Manon
IPC分类号： C12N7/01 , A01H5/00 , A61K39/205 , A61P31/14 , C07K14/145 , C07K16/10 , C12N15/00 , C12N15/47 , C12N15/82 , C12N7/00
CPC分类号： C07K14/005 , A61K39/12 , A61K39/205 , A61K2039/517 , A61K2039/5258 , A61K2039/55505 , C12N15/8258 , C12N2750/12043 , C12N2760/20123 , C12N2760/20134
摘要： A method of producing a virus like particle (VLP) in a plant is provided. The method comprises introducing a first nucleic acid into the plant, or portion of the plant. The first nucleic acid comprising a first regulatory region active in the plant operatively linked to a nucleotide sequence encoding a native rabies virus structural protein. The nucleotide sequence may further comprise one or more than one amplification element. Optionally, a second nucleic acid might be introduced into the plant, or portion of the plant. The second nucleic acid comprising a second regulatory region active in the plant and operatively linked to a nucleotide sequence encoding a matrix protein, for example but not limited to a rabies matrix protein. The plant or portion of the plant is incubated under conditions that permit the expression of the nucleic acids, thereby producing the VLP.
摘要翻译：提供了一种在植物中生产病毒样颗粒（VLP）的方法。该方法包括将第一核酸引入植物或植物的一部分。所述第一核酸包含在植物中活性的第一调节区，其与编码天然狂犬病毒结构蛋白的核苷酸序列有效连接。核苷酸序列还可以包含一个或多于一个的扩增元件。任选地，第二核酸可以被引入植物或植物的一部分。所述第二核酸包含在植物中活性的第二调节区，并与编码基质蛋白（例如但不限于狂犬病基质蛋白）的核苷酸序列有效连接。将植物或植物的一部分在允许核酸表达的条件下温育，从而产生VLP。

4. 发明申请

WO2012168794A1 AFFINITY TRAPPING AGENT AND USES THEREOF 审中-公开
标题翻译： AFFINITY TRAPPING代理及其用途
公开(公告)号：WO2012168794A1
公开(公告)日：2012-12-13
申请号：PCT/IB2012/001268
申请日：2012-06-06
申请人： ANGANY GENETICS , MEDICAGO, INC. , GOMORD, Véronique , FAYE, Loïc , D'AOUST, Marc-André , COUTURE, Manon , TREPANIER, Sonia , VEZINA, Louis-Philippe , FITCHETTE, Anne Catherine
发明人： GOMORD, Véronique , FAYE, Loïc , D'AOUST, Marc-André , COUTURE, Manon , TREPANIER, Sonia , VEZINA, Louis-Philippe , FITCHETTE, Anne Catherine
IPC分类号： C12N15/82
CPC分类号： C12N15/8258 , C12N15/8257
摘要： A nucleic acid encoding an affinity trapping agent is provided. The affinity trapping agent comprises a peptide or protein ligand having a binding affinity for a protein of interest, fused to a heterologous subcellular compartment targeting signal. Methods for targeting the immobilization of a recombinant peptide or protein of interest, or an endogenous peptide or protein of interest, to a subcellular compartment of a cell are also provided. If the peptide or protein of interest is recombinant then a nucleotide sequence encoding the recombinant peptide or protein of interest and a nucleic acid encoding the affinity trapping agent are introduced into the cell and co- expressed to form a complex thereby targeting the immobilization of the recombinant peptide or protein of interest. If the peptide or protein of interest is endogenous, then the nucleic acid encoding the affinity trapping agent having a binding affinity for the endogenous peptide or protein of interest is introduced into the cell, and the nucleic acid encoding the affinity trapping agent is expressed in the cell to form a complex thereby targeting immobilization of the endogenous peptide or protein of interest.
摘要翻译：提供编码亲和性捕获剂的核酸。亲和捕获剂包括与目标蛋白质结合亲和力的肽或蛋白质配体，其与异源亚细胞区室靶向信号融合。还提供了将目标重组肽或目的蛋白或感兴趣的内源性肽或蛋白质固定靶向细胞亚细胞室的方法。如果感兴趣的肽或重组蛋白是重组的，则将编码目标重组肽或蛋白质的核苷酸序列和编码亲和捕获剂的核酸引入细胞并共表达以形成复合物，从而靶向重组体肽或目的蛋白质。如果目的肽或蛋白质是内源性的，则将编码对所述目的内源肽或蛋白质具有结合亲和力的亲和捕获剂的核酸引入细胞中，并且编码亲和捕获剂的核酸在细胞以形成复合物，从而靶向目标的内源性肽或蛋白质的固定化。

5. 发明申请

WO2012083445A8 VIRUS LIKE PARTICLE PRODUCTION IN PLANTS 审中-公开
标题翻译：病毒样品在植物中的生产
公开(公告)号：WO2012083445A8
公开(公告)日：2012-10-04
申请号：PCT/CA2011001427
申请日：2011-12-22
申请人： MEDICAGO INC , D AOUST MARC-ANDRE , COUTURE MANON , LAVOIE PIERRE-OLIVIER , VEZINA LOUIS-PHILIPPE
发明人： D AOUST MARC-ANDRE , COUTURE MANON , LAVOIE PIERRE-OLIVIER , VEZINA LOUIS-PHILIPPE
IPC分类号： C12N15/00 , A61K39/295 , A61P31/16 , A61P37/04 , C07K14/005 , C07K14/11 , C07K19/00 , C12N7/00 , C12N15/62 , C12N15/82
CPC分类号： A61K39/295 , A61K2039/5258 , C07K14/005 , C07K2319/03 , C12N7/00 , C12N15/8257 , C12N15/8258 , C12N2710/16723 , C12N2710/16751 , C12N2740/16023 , C12N2740/16034 , C12N2740/16051 , C12N2740/16134 , C12N2760/14123 , C12N2760/14134 , C12N2760/14151 , C12N2760/16123 , C12N2760/20123 , C12N2760/20134 , C12N2760/20151 , C12N2770/20023 , C12N2770/20034 , C12N2770/20051
摘要： A method of producing a virus like particle (VLP) in a plant, and compositions comprising VLPs, are provided. The method involves introducing a nucleic acid comprising a regulatory region active in the plant and operatively linked to a chimeric nucleotide sequence encoding, in series, an ectodomain from a virus trimeric surface protein or fragment thereof, fused to an influenza transmembrane domain and cytoplasmic tail, into the plant, or portion of the plant, the ectodomain is from a non- influenza virus trimeric surface protein and heterologous with respect to the influenza transmembrane domain, and the cytoplasmic tail. The plant or portion of the plant are incubated under conditions that permit the expression of the nucleic acid, thereby producing the VLP. A VLP produced by this method are also provided.
摘要翻译：提供了一种在植物中生产病毒样颗粒（VLP）的方法，以及包含VLP的组合物。该方法包括引入包含在植物中有活性的调节区的核酸，并可操作地连接到嵌合核苷酸序列，该嵌合核苷酸序列串联编码与流感跨膜结构域和细胞质尾部融合的病毒三聚体表面蛋白或其片段的胞外域，植物或植物的一部分，胞外域是来自非流感病毒三聚体表面蛋白，并且与流感跨膜结构域和细胞质尾部是异源的。植物或植物的一部分在允许核酸表达的条件下温育，从而产生VLP。还提供了通过该方法生产的VLP。

6. 发明申请

WO2010003225A8 INFLUENZA VIRUS-LIKE PARTICLES (VLPS) COMPRISING HEMAGGLUTININ 审中-公开
标题翻译：包含HEMAGGLUTININ的流感病毒颗粒（VLPS）
公开(公告)号：WO2010003225A8
公开(公告)日：2010-07-22
申请号：PCT/CA2009000926
申请日：2009-07-02
申请人： MEDICAGO INC , D AOUST MARC-ANDRE , COUTURE MANON , ORS FREDERIC , TREPANIER SONIA , LAVOIE PIERRE-OLIVIER , DARGIS MICHELE , VEZINA LOUIS-PHILIPPE , LANDRY NATHALIE
发明人： D AOUST MARC-ANDRE , COUTURE MANON , ORS FREDERIC , TREPANIER SONIA , LAVOIE PIERRE-OLIVIER , DARGIS MICHELE , VEZINA LOUIS-PHILIPPE , LANDRY NATHALIE
IPC分类号： C12N15/82 , A61K39/145 , A61P31/16 , C07K14/11 , C12N7/00 , C12N7/01 , C12N15/44
CPC分类号： C12N15/8257 , A61K39/12 , A61K39/145 , A61K2039/5258 , A61K2039/543 , A61K2039/55505 , A61K2039/55583 , A61K2039/58 , C07K14/005 , C07K2319/02 , C12N7/00 , C12N2760/16122 , C12N2760/16123 , C12N2760/16134 , C12N2830/65
摘要： A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of influenza HA of type A/California/04/09 in plants and the purification by size exclusion chromatography. The invention is also directed towards a VLP comprising influenza HA protein of type A/California/04/09 and plants lipids. The invention is also directed to a nucleic acid encoding influenza HA of type A/California/04/09 as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.
摘要翻译：提供了在植物或植物的一部分内合成流感病毒样颗粒（VLP）的方法。该方法包括在植物中表达A / California / 04/09型流感病毒HA，并通过大小排阻色谱法纯化。本发明还涉及包含A / California / 04/09型流感HA蛋白和植物脂质的VLP。本发明还涉及编码A / California / 04/09型流感病毒的核酸以及载体。 VLP可用于制定流感疫苗，或可用于丰富现有疫苗。

7. 发明申请

WO2010003225A1 INFLUENZA VIRUS-LIKE PARTICLES (VLPS) COMPRISING HEMAGGLUTININ 审中-公开
标题翻译：包含血凝素的流感病毒样颗粒（VLPS）
公开(公告)号：WO2010003225A1
公开(公告)日：2010-01-14
申请号：PCT/CA2009/000926
申请日：2009-07-02
申请人： MEDICAGO, INC. , D'AOUST, Marc-Andre , COUTURE, Manon , ORS, Frédéric , TREPANIER, Sonia , LAVOIE, Pierre-Olivier , DARGIS, Michèle , VEZINA, Louis-Philippe , LANDRY, Nathalie
发明人： D'AOUST, Marc-Andre , COUTURE, Manon , ORS, Frédéric , TREPANIER, Sonia , LAVOIE, Pierre-Olivier , DARGIS, Michèle , VEZINA, Louis-Philippe , LANDRY, Nathalie
IPC分类号： C12N15/82 , A61K39/145 , A61P31/16 , C12N15/44 , C12N7/00 , C12N7/01 , C07K14/11
CPC分类号： C12N15/8257 , A61K39/12 , A61K39/145 , A61K2039/5258 , A61K2039/543 , A61K2039/55505 , A61K2039/55583 , A61K2039/58 , C07K14/005 , C07K2319/02 , C12N7/00 , C12N2760/16122 , C12N2760/16123 , C12N2760/16134 , C12N2830/65
摘要： A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of influenza HA of type A/California/04/09 in plants and the purification by size exclusion chromatography. The invention is also directed towards a VLP comprising influenza HA protein of type A/California/04/09 and plants lipids. The invention is also directed to a nucleic acid encoding influenza HA of type A/California/04/09 as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.
摘要翻译：提供了用于在植物或植物的一部分内合成流感病毒样颗粒（VLP）的方法。该方法涉及在植物中表达A型/ California / 04/09型流感HA并通过尺寸排阻色谱法进行纯化。本发明还涉及包含A / California / 04/09型流感HA蛋白和植物脂质的VLP。本发明还涉及编码A / California / 04/09型流感HA的核酸以及载体。 VLP可用于配制流感疫苗，或可用于丰富现有疫苗。

8. 发明申请

WO2010003235A1 SOLUBLE RECOMBINANT INFLUENZA ANTIGENS 审中-公开
标题翻译：可溶性重组流感病毒抗体
公开(公告)号：WO2010003235A1
公开(公告)日：2010-01-14
申请号：PCT/CA2009/000941
申请日：2009-07-07
申请人： MEDICAGO INC. , COUTURE, Manon , LANDRY, Nathalie , VEZINA, Louis-Philippe , DARGIS, Michèle
发明人： COUTURE, Manon , LANDRY, Nathalie , VEZINA, Louis-Philippe , DARGIS, Michèle
IPC分类号： C12N15/62 , A61K39/145 , A61K39/385 , C07K14/11 , C07K19/00 , C12N15/44 , C12N15/82 , C12P21/02
CPC分类号： C12N15/8257 , A61K39/00 , A61K39/12 , A61K39/145 , C07K14/005 , C07K2319/50 , C07K2319/70 , C07K2319/73 , C07K2319/735 , C12N2760/16122 , C12N2760/16134
摘要： The present invention provides a recombinant soluble trimeric hemagglutinin (rHA) protein comprising a hemagglutinin ectodomain and an oligomerization domain. The rHA is produced as a soluble homotrimer, and may further comprises a signal peptide and/or an endoplasmic reticulum (ER) retention signal. The invention is also directed to nucleic acids encoding the rHA of the invention, as well as vectors and chimeric constructs comprising the nucleic acid. Methods of producing the rHA are also provided. The rHA described herein may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.
摘要翻译：本发明提供了包含血凝素胞外域和寡聚化结构域的重组可溶性三聚体血凝素（rHA）蛋白。 rHA产生为可溶性同三聚物，并且还可以包含信号肽和/或内质网（ER）保留信号。本发明还涉及编码本发明的rHA的核酸，以及包含该核酸的载体和嵌合构建体。还提供了生产rHA的方法。本文所述的rHA可用于制定流感疫苗，或可用于富集现有疫苗。

9. 发明申请

WO2009076778A8 RECOMBINANT INFLUENZA VIRUS-LIKE PARTICLES (VLPS) PRODUCED IN TRANSGENIC PLANTS EXPRESSING HEMAGGLUTININ 审中-公开
标题翻译：转基因植物中生产的重组流感病毒样芽孢杆菌（VLPS）表达HEMAGGLUTININ
公开(公告)号：WO2009076778A8
公开(公告)日：2009-08-27
申请号：PCT/CA2009000032
申请日：2009-01-12
申请人： MEDICAGO INC , D AOUST MARC-ANDRE , COUTURE MANON , ORS FREDERIC , TREPANIER SONIA , LAVOIE PIERRE-OLIVIER , DARGIS MICHELE , VEZINA LOUIS-PHILIPPE , LANDRY NATHALIE
发明人： D AOUST MARC-ANDRE , COUTURE MANON , ORS FREDERIC , TREPANIER SONIA , LAVOIE PIERRE-OLIVIER , DARGIS MICHELE , VEZINA LOUIS-PHILIPPE , LANDRY NATHALIE
IPC分类号： C12N15/82 , A01H5/00 , A23L33/00 , A61K35/76 , A61K39/145 , A61P31/16 , A61P37/04 , C07K14/11 , C12N7/00 , C12N7/01 , C12N15/44
CPC分类号： C12N15/8258 , A61K39/12 , A61K39/145 , A61K2039/5258 , A61K2039/543 , A61K2039/55505 , A61K2039/55583 , A61K2039/55588 , A61K2039/58 , C07K14/005 , C07K2319/02 , C07K2319/03 , C12N7/00 , C12N9/90 , C12N15/8257 , C12N2760/16022 , C12N2760/16122 , C12N2760/16123 , C12N2760/16133 , C12N2760/16134 , C12N2760/16151 , C12N2760/16171 , C12N2760/16222 , C12N2760/16223 , C12N2760/16234 , C12Y503/04001 , G06F9/505 , G06F2209/508
摘要： A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of influenza HA in plants and the purification by size exclusion chromatography. The invention is also directed towards a VLP comprising influenza HA protein and plants lipids. The invention is also directed to a nucleic acid encoding influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.
摘要翻译：提供了在植物或植物的一部分内合成流感病毒样颗粒（VLP）的方法。该方法包括在植物中表达流感HA并通过尺寸排阻色谱法进行纯化。本发明还涉及包含流感HA蛋白和植物脂质的VLP。本发明还涉及编码流感HA以及载体的核酸。 VLP可用于制定流感疫苗，或可用于丰富现有疫苗。

10. 发明申请

WO2009076778A1 RECOMBINANT INFLUENZA VIRUS-LIKE PARTICLES (VLPS) PRODUCED IN TRANSGENIC PLANTS EXPRESSING HEMAGGLUTININ 审中-公开
标题翻译：重组流感病毒表达血凝素的重组流感病毒样颗粒（VLPS）
公开(公告)号：WO2009076778A1
公开(公告)日：2009-06-25
申请号：PCT/CA2009/000032
申请日：2009-01-12
申请人： MEDICAGO INC. , D'AOUST, Marc-André , COUTURE, Manon , ORS, Frédéric , TRÉPANIER, Sonia , LAVOIE, Pierre-Olivier , DARGIS, Michèle , VÉZINA, Louis-Philippe , LANDRY, Nathalie
发明人： D'AOUST, Marc-André , COUTURE, Manon , ORS, Frédéric , TRÉPANIER, Sonia , LAVOIE, Pierre-Olivier , DARGIS, Michèle , VÉZINA, Louis-Philippe , LANDRY, Nathalie
IPC分类号： C12N15/82 , A01H5/00 , A61K35/76 , A61K39/145 , A61P31/16 , A61P37/04 , C07K14/11 , C12N7/00 , C12N15/44 , C12N7/01
CPC分类号： C12N15/8258 , A61K39/12 , A61K39/145 , A61K2039/5258 , A61K2039/543 , A61K2039/55505 , A61K2039/55583 , A61K2039/55588 , A61K2039/58 , C07K14/005 , C07K2319/02 , C07K2319/03 , C12N7/00 , C12N9/90 , C12N15/8257 , C12N2760/16022 , C12N2760/16122 , C12N2760/16123 , C12N2760/16133 , C12N2760/16134 , C12N2760/16151 , C12N2760/16171 , C12N2760/16222 , C12N2760/16223 , C12N2760/16234 , C12Y503/04001 , G06F9/505 , G06F2209/508
摘要： A method for synthesizing influenza virus-like particles (VLPs) within a plant or a portion of a plant is provided. The method involves expression of influenza HA in plants and the purification by size exclusion chromatography. The invention is also directed towards a VLP comprising influenza HA protein and plants lipids. The invention is also directed to a nucleic acid encoding influenza HA as well as vectors. The VLPs may be used to formulate influenza vaccines, or may be used to enrich existing vaccines.
摘要翻译：提供了用于在植物或植物的一部分内合成流感病毒样颗粒（VLP）的方法。该方法涉及在流感病毒中表达流感HA并通过尺寸排阻色谱法进行纯化。本发明还涉及包含流感HA蛋白和植物脂质的VLP。本发明还涉及编码流感HA的核酸以及载体。 VLP可用于配制流感疫苗，或可用于丰富现有疫苗。

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