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    • 2. 发明申请
    • DETECTION OF CANCER AND ABNORMAL PREGNANCY USING MONOCLONAL ANTIBODIES SPECIFIC FOR HCG ISOFORMS
    • 用HCG ISOFORMS特异性单克隆抗体检测癌症和异常性前列腺炎
    • WO0061638A3
    • 2001-04-19
    • PCT/US0009776
    • 2000-04-12
    • UNIV COLUMBIAKRICHEVSKY ALEXANDERBIRKEN STEVENCONNOR JOHN O
    • KRICHEVSKY ALEXANDERBIRKEN STEVENO'CONNOR JOHN
    • C07K16/26G01N33/574G01N33/76
    • C07K16/26
    • Human chorionic gonadotropin (hCG) exists in blood and urine as a variety of isoforms, one of which contains peptide bond cleavages within its beta subunit and is referred to as nicked hCG (hCGn). This hCGn isoform appears more prevalent in the urine of patients with certain malignancies and possibly in other diseases of pregnancy. The present invention is directed to two monoclonal antibodies to an isoform of hCGn isolated from a choriocarcinoma patient. Two-site immunometric assays have been developed using these antibodies, designated B151 and B152. The former exhibits good specificity for hCGn independent of the source of the hCGn, that form excreted by choriocarcinoma patients, or the form of hCGn from normal pregnancies. The latter antibody, B152, is uniquely sensitive to the carbohydrate moieties of choriocarcinoma hCG, nicked or non-nicked, since its recognition of ligand is dependent upon carbohydrate differences rather than differences in peptide bond. These two immunometric assays provide novel diagnostic tools based on direct measurement of these hCG isoforms.
    • 人绒毛膜促性腺激素(hCG)存在于血液和尿液中作为多种同工型,其中一种在其β亚基内含有肽键裂解,称为切口hCG(hCGn)。 这种hCGn同种型在某些恶性肿瘤患者和可能在其他妊娠疾病患者的尿液中更为普遍。 本发明涉及从绒毛膜癌患者分离的hCGn同种型的两种单克隆抗体。 已经开发了使用这些抗体(称为B151和B152)的两位点免疫测定法。 前者对hCGn具有良好的特异性,独立于hCGn的来源,由绒毛膜癌患者排出,或正常妊娠的hCGn形式。 后一种抗体B152对于具有切口或非切口的绒毛膜癌hCG的碳水化合物部分是独特的,因为其识别配体取决于碳水化合物差异而不是肽键的差异。 这两种免疫测定提供了基于直接测量这些hCG同种型的新型诊断工具。