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2. 发明申请

WO2004099409A8 PROCESS FOR EXTRACTING NUCLEIC ACID 审中-公开
标题翻译：提取核酸的方法
公开(公告)号：WO2004099409A8
公开(公告)日：2005-01-13
申请号：PCT/CN2004000177
申请日：2004-03-05
申请人： CI XI SHI ZHONG DING BIOTECHNO , CHEN HUI
发明人： CHEN HUI
IPC分类号： C07H1/06 , C07H21/00 , C12N15/10
CPC分类号： C07H1/06 , C07H21/00 , C12N15/1006
摘要： The invention provides a process for isolating biomaterial from other materials, especially isolating nucleic acid such as DNA or RNA or hybrid molecule of DNA and RNA. The invention also provides a process for purifying nucleic acid from biomaterials, especially purifying DNA. A siliceous material combined with a new solution is used in the process to prepare high pure biomaterial, especially DNA. Therein the siliceous material is a support for absorbing target materials, and the new solution according to this invention is to promote the target materials to bind to the siliceous material, especially to promote DNA to bind to the siliceous material. The solution is a acid and potassium ion-containing aqueous solution. The invention further provides a kit using the process, in which an amount of siliceous material and the solution and other needed agents or solutions are contained. The invention further provides DNA prepared by using the process. Because no chaotropic agents or other poisonous or costly agents are used in the process, DNA prepared by the process may be used widely, especially in food industry and pharmaceutical industry.
摘要翻译：本发明提供了从其他材料分离生物材料的方法，特别是分离核酸如DNA或RNA或DNA和RNA的混合分子。本发明还提供从生物材料中纯化核酸的方法，特别是纯化DNA。在该过程中使用与新溶液结合的硅质材料来制备高纯度生物材料，特别是DNA。其中硅质材料是用于吸收目标材料的载体，根据本发明的新溶液是促进目标材料结合硅质材料，特别是促进DNA结合硅质材料。该溶液是含酸和钾离子的水溶液。本发明还提供了一种使用该方法的试剂盒，其中含有一定量的硅质材料和溶液以及其它所需的试剂或溶液。本发明还提供了使用该方法制备的DNA。由于在该过程中不使用离液剂或其他有毒或昂贵的药剂，所以通过该方法制备的DNA可以广泛使用，特别是在食品工业和制药工业中。

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