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1. 发明授权

EP1567569B1 METHOD FOR IMMOBILIZING BIOLOGIC MOLECULES ON SOLID SURFACES 有权
标题翻译：方法的生物分子在固体表面上固定化
公开(公告)号：EP1567569B1
公开(公告)日：2008-07-23
申请号：EP02790459.8
申请日：2002-11-29
申请人： Chiari, Marcella
发明人： Chiari, Marcella
IPC分类号： C08F220/56 , C08F246/00 , B01J19/00 , C12N11/08
CPC分类号： C07K17/08 , B01J19/0046 , B01J2219/00608 , B01J2219/00637 , C08F220/32 , C08F220/56 , C08F220/60 , C08F226/06 , C09D139/04 , G01N33/531 , G01N33/54353

2. 发明授权

EP1218426B1 DYNAMIC COATING 有权
标题翻译：动态涂料
公开(公告)号：EP1218426B1
公开(公告)日：2006-01-04
申请号：EP00960498.4
申请日：2000-08-21
申请人： Chiari, Marcella
发明人： Chiari, Marcella
IPC分类号： C08F220/54 , G01N27/447
CPC分类号： G01N27/44752 , C08F220/54 , C08F220/56 , C12N9/1252 , C12Q1/6869 , C12Q2535/101 , C12Q2531/113 , C12Q2521/101
摘要： The instant invention describes uncharged water-soluble polymers to suppress electroosmotic flow in capillary electrophoresis. The polymers in the instant invention are copolymers of various derivatives of acrylamide and methacrylamide monomers with various glycidyl group containing monomers e.g., dimethylacrylamide and allyl glycidyl ether -epoxy poly (DMA)-, copolymers of various derivatives of acrylamide and methacrylamide with various allyl group containing carbohydrates and various glycidyl group containing monomers, such as allyl β-D-pyranoside (typically β-D-Glucopyranoside) or allyl β-D-furanoside, allyl glycidyl ether -epoxy poly (AG-AA) and copolymers of four different monomers including various acryl and methacrylamide, various allyl group containing carbohydrates, various glycidyl group containing monomer and various diol group containing monomer, such as acrylamide, allyl -βD-pyranoside (typically β-D-galactopyranoside or N-allylgluconamide) or allyl β-D furanoside, allyl glycydyl ether and allyoxy-1,2 propanediol -epoxy poly (AGal-AA-APD). The subject polymers adsorb onto the capillary surface, forming a highly hydrophilic, dynamic coating that suppresses electroosmotic flow. The subject polymers may be used as a coating prior to use of an electrophoresis microchannel or may be included in a separation media contained within the capillary column. The instant coatings and media are especially suitable for applications pertaining to the electrophoretic separation of various biomelecules, such as protein and DNA.

3. 发明公开

EP1567569A1 METHOD FOR IMMOBILIZING BIOLOGIC MOLECULES ON SOLID SURFACES 有权
标题翻译：方法的生物分子在固体表面上固定化
公开(公告)号：EP1567569A1
公开(公告)日：2005-08-31
申请号：EP02790459.8
申请日：2002-11-29
申请人： Chiari, Marcella
发明人： Chiari, Marcella
IPC分类号： C08F220/56 , C08F246/00 , B01J19/00 , C12N11/08
CPC分类号： C07K17/08 , B01J19/0046 , B01J2219/00608 , B01J2219/00637 , C08F220/32 , C08F220/56 , C08F220/60 , C08F226/06 , C09D139/04 , G01N33/531 , G01N33/54353
摘要： The invention provides a method for immobilization of biological molecules such as nucleic acids, peptides and proteins onto the surface of a glass or plastic solid support.

4. 发明申请

WO2018178943A1 GENOTYPING OF MUTATIONS BY COMBINATION OF IN-TUBE HYBRIDIZATION AND UNIVERSAL TAG-MICROARRAY 审中-公开
公开(公告)号：WO2018178943A1
公开(公告)日：2018-10-04
申请号：PCT/IB2018/052219
申请日：2018-03-30
申请人： CHIARI, Marcella , DAMIN, Francesco , GALBIATI, Silvia , FERRARI, Maurizio
发明人： CHIARI, Marcella , DAMIN, Francesco , GALBIATI, Silvia , FERRARI, Maurizio
IPC分类号： C12Q1/6827 , C12Q1/6837
摘要： An assay method for a gene fragment of interest comprising: amplify at least one gene fragment of interest comprising or potentially comprising at least one SNP region of interest to form an initial amplification product; forming at least one single strand amplification product from the initial amplification product, wherein the single strand amplification product either comprises or potentially comprises the at least one SNP region of interest; hybridize in solution the single strand amplification product with at least one reporter molecule which comprises at least two, different domains of oligonucleotide, wherein a first domain of oligonucleotide is for hybridization with the single strand amplification product, and wherein the second domain of oligonucleotide is for hybridization to at least one microarray probe surface, wherein the microarray probe surface comprises at least one capture probe to allow for hybridization; contact the solution of the hybridized single strand amplification product with the at least one microarray probe surface which comprises at least one capture probe to allow for hybridization; detect for the presence of the hybridized single strand amplification product on the microarray surface. In the preferred embodiment relevant to cancer genotyping, the unexpectedly good results for KRAS oncogene indicate that all the seven codon 12 and 13 mutations studied could be unambiguously detected in less than 90 minutes in tissue clinical samples. Moreover, this system could reveal mutant alleles representing less than 0.1% of the starting material. By decoupling the mutation detection from the array hybridization, this technology becomes universal.

5. 发明申请

WO2016092372A1 NEW CLICKABLE POLYMERS AND GELS FOR MICROARRAY AND OTHER APPLICATIONS 审中-公开
标题翻译：新的点胶聚合物和微胶囊和其他应用的凝胶
公开(公告)号：WO2016092372A1
公开(公告)日：2016-06-16
申请号：PCT/IB2015/002470
申请日：2015-12-11
申请人： CHIARI, Marcella
发明人： CHIARI, Marcella
IPC分类号： C09D133/26 , C08F220/40 , C08F220/54 , C08F220/56 , C08F230/08 , C09D143/04
CPC分类号： C09D143/04 , C07H1/06 , C07H21/04 , C08F220/54 , C08L2203/02 , C08L2312/08 , C09D133/24 , C09D133/26 , G01N33/54353 , G01N33/545 , C08F2230/085 , C08F230/08
摘要： Fabrication of arrays, including glycan arrays, that combines the higher sensitivity of a layered Si-SiO 2 substrate with novel immobilization chemistry via a "click" reaction. The novel immobilization approach allows the oriented attachment of glycans on a "clickable" polymeric coating. The surface equilibrium dissociation constant (K D ) of Concanavalin A with eight synthetic glycans was determined using fluorescence microarray. The sensitivity provided by the novel microarray substrate enables the evaluation of the influence of the glycan surface density on surface K D values. The interaction of carbohydrates with a variety of biological targets, including antibodies, proteins, viruses and cells are of utmost importance in many aspects of biology. Glycan microarrays are increasingly used to determine the binding specificity of glycan-binding proteins. The click polymers can be prepared in different forms such as soluble polymers, hydrogels, and multi-layers. The polymers can be prepared directly by copolymerization or by copolymerization to form a pre-polymer which is then reacted to form the target polymer. Other uses include separations, including electrophoretic separations.
摘要翻译：通过“点击”反应，组合阵列的阵列，其将层状Si-SiO 2衬底的较高灵敏度与新颖的固定化学反应结合在一起。新型固定化方法允许将聚糖定向附着在“可点击”的聚合物涂层上。使用荧光微阵列测定具有8个合成聚糖的伴刀豆球蛋白A的表面平衡解离常数（KD）。由新型微阵列基板提供的灵敏度能够评估聚糖表面密度对表面KD值的影响。碳水化合物与各种生物靶标（包括抗体，蛋白质，病毒和细胞）的相互作用在生物学的许多方面是至关重要的。聚糖微阵列越来越多地用于确定聚糖结合蛋白的结合特异性。点击聚合物可以以不同形式制备，例如可溶性聚合物，水凝胶和多层。聚合物可以通过共聚合或通过共聚直接制备形成预聚物，然后反应形成目标聚合物。其他用途包括分离，包括电泳分离。

6. 发明申请

WO2006128644A1 POLYMER COATING AND FUNCTIONALIZATION OF SOLID SURFACES 审中-公开
标题翻译：聚合物涂层和固体表面的功能化
公开(公告)号：WO2006128644A1
公开(公告)日：2006-12-07
申请号：PCT/EP2006/005047
申请日：2006-05-26
申请人： PIRRI, Giovanna , CHIARI, Marcella
发明人： PIRRI, Giovanna , CHIARI, Marcella
IPC分类号： C03C17/30
CPC分类号： G01N33/54353 , C03C17/30 , G01N33/545
摘要： Disclosed is a method for coating glass, silica, silicon-oxide or other materials with brush polymers obtained by RAFT polymerization. The coated surfaces are used to immobilize biomolecules especially for microarray technology applications.
摘要翻译：公开了通过RAFT聚合获得的刷式聚合物涂覆玻璃，二氧化硅，氧化硅或其它材料的方法。涂覆的表面用于固定生物分子，特别是用于微阵列技术应用。

7. 发明申请

WO2021044381A1 CONJUGATES COMPOSED OF MEMBRANE-TARGETING PEPTIDES FOR EXTRACELLULAR VESICLES ISOLATION, ANALYSIS AND THEIR INTEGRATION THEREOF 审中-公开
公开(公告)号：WO2021044381A1
公开(公告)日：2021-03-11
申请号：PCT/IB2020/058284
申请日：2020-09-04
申请人： GORI, Alessandro , CRETICH, Marina , CHIARI, Marcella
发明人： GORI, Alessandro , CRETICH, Marina , CHIARI, Marcella
IPC分类号： G01N33/543 , G01N21/00
摘要： A molecular probe comprising at least one first moiety and at least one second moiety which are each covalently bound to the molecular probe, wherein: the at least one first moiety comprises at least one binding peptide or peptidomimetic for binding the molecular probe to at least one extracellular vesicle (EV) membrane, wherein the binding is mediated by EV membrane curvature sensing; and the at least one second moiety comprises at least one support binding group for binding of the molecular probe to at least one support. The molecular probe can also comprise a spacer moiety, and/or a labeling moiety, and/or a modification moiety. The molecular probe can be bound to a solid or semi-solid support including a microarray support. The molecular probe can be used in methods in which it is contacted with sample which might include the extracellular vesicle and can involve detection and isolation steps. Kits can include the molecular probe.

8. 发明申请

WO2004050729A1 METHOD FOR IMMOBILIZING BIOLOGIC MOLECULES ON SOLID SURFACES 审中-公开
标题翻译：固定表面生物分子的方法
公开(公告)号：WO2004050729A1
公开(公告)日：2004-06-17
申请号：PCT/EP2002/013513
申请日：2002-11-29
申请人： CHIARI, Marcella
发明人： CHIARI, Marcella
IPC分类号： C08F220/56
CPC分类号： C07K17/08 , B01J19/0046 , B01J2219/00608 , B01J2219/00637 , C08F220/32 , C08F220/56 , C08F220/60 , C08F226/06 , C09D139/04 , G01N33/531 , G01N33/54353
摘要： The invention provides a method for immobilization of biological molecules such as nucleic acids, peptides and proteins onto the surface of a glass or plastic solid support.
摘要翻译：本发明提供了将生物分子例如核酸，肽和蛋白质固定在玻璃或塑料固体支持物的表面上的方法。

9. 发明申请

WO2011124715A1 SILANE COPOLYMERS AND USES THEREOF 审中-公开
标题翻译：硅烷共聚物及其用途
公开(公告)号：WO2011124715A1
公开(公告)日：2011-10-13
申请号：PCT/EP2011/055574
申请日：2011-04-08
申请人： CHIARI, Marcella
发明人： CHIARI, Marcella
IPC分类号： C08F220/56 , C08F224/00 , C08F230/08 , C09D133/26 , C09D137/00 , C09D143/04 , G01N27/447 , B01L3/00
CPC分类号： C09D179/08 , B01L3/5027 , B01L3/502707 , B01L3/502753 , C08F220/56 , C08F224/00 , C08F230/08 , C09D133/26 , C09D137/00 , C09D143/04 , C09D163/10 , G01N27/44704
摘要： The invention relates to a method to reduce the electroosmotic flow in a capillary or in a channel and to a method to reduce the attachment of biological species to a surface comprising the step of coating said capillary, channel or surface with a copolymer comprising a surface interacting monomer, a monomer bearing a chemically active group and an ethylenically unsaturated silane monomer.
摘要翻译：本发明涉及减少毛细管或通道中的电渗流的方法，以及减少生物物质附着到表面的方法，包括用包含表面相互作用的共聚物涂覆所述毛细管，通道或表面的步骤单体，带有化学活性基团的单体和烯属不饱和硅烷单体。

10. 发明公开

EP4025911A1 CONJUGATES COMPOSED OF MEMBRANE-TARGETING PEPTIDES FOR EXTRACELLULAR VESICLES ISOLATION, ANALYSIS AND THEIR INTEGRATION THEREOF 有权转让
公开(公告)号：EP4025911A1
公开(公告)日：2022-07-13
申请号：EP20797166.4
申请日：2020-09-04
申请人： Gori, Alessandro , Cretich, Marina , Chiari, Marcella
发明人： Gori, Alessandro , Cretich, Marina , Chiari, Marcella
IPC分类号： G01N33/543 , G01N21/00

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