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1. 发明申请

WO2009158591A1 STRONG ACTIVATION DOMAIN 审中-公开
标题翻译：强活动域
公开(公告)号：WO2009158591A1
公开(公告)日：2009-12-30
申请号：PCT/US2009/048814
申请日：2009-06-26
申请人： MENDEL BIOTECHNOLOGY, INC. , TIWARI, Shiv , CANALES, Roger , REUBER, T., Lynne , CENTURY, Karen, S. , RATCLIFFE, Oliver
发明人： TIWARI, Shiv , CANALES, Roger , REUBER, T., Lynne , CENTURY, Karen, S. , RATCLIFFE, Oliver
IPC分类号： C07K14/415 , C07H21/00
CPC分类号： C12N15/8216 , C07K14/415 , C07K2319/00 , C12N15/8251 , C12N15/8261 , Y02A40/146
摘要： A new and strong transcriptional activation domain was identified from the Arabidopsis protein Ethylene Response Factor 98 (AtERF98). This domain has been designated as the "EDLL domain" and has a number of highly conserved amino acid residues that are found throughout the members of the AtERF98 family from plants, including in monocot and eudicot orthologs. The EDLL domain was shown to be highly active when it was fused to transcription factors from plant and yeast, and was also shown to have activation potential comparable to the widely-used VP16 activation domain derived from Herpes simplex. The EDLL domain was also active when it was targeted to a gene promoter by a sequence-specific DNA binding protein or by protein-protein interactions. Unlike other known activation domains such as VP16 and GAL4, the EDLL domain is relatively small in size, and being of plant origin, it is favored as a strong transcriptional activation tool for application in transgenic food crops.
摘要翻译：从拟南芥蛋白质乙烯响应因子98（AtERF98）中鉴定出新的和强的转录激活结构域。该领域已经被指定为“EDLL结构域”，并且具有许多高度保守的氨基酸残基，其在来自植物的AtERF98家族的整个成员中发现，包括单子叶植物和雌蕊直系同源物。当EDLL结构域与植物和酵母的转录因子融合时，显示其具有高度的活性，并且还显示具有与广泛使用的衍生自单纯疱疹的VP16活化区域相当的活化潜力。当通过序列特异性DNA结合蛋白或通过蛋白质 - 蛋白质相互作用靶向基因启动子时，EDLL结构域也是活性的。与其他已知的活化结构域（如VP16和GAL4）不同，EDLL结构域的大小相对较小，并且是植物来源的，因此作为在转基因食品作物中应用的强转录激活工具是有利的。

2. 发明申请

WO2007120820A3 PLANT DISEASE RESISTANCE GENES AND PROTEINS 审中-公开
标题翻译：植物抗病基因和蛋白质
公开(公告)号：WO2007120820A3
公开(公告)日：2008-12-11
申请号：PCT/US2007009124
申请日：2007-04-12
申请人： MENDEL BIOTECHNOLOGY INC , REUBER T LYNNE , CENTURY KAREN S
发明人： REUBER T LYNNE , CENTURY KAREN S
IPC分类号： C12N15/09 , A01H5/00 , A01H5/10 , C12N15/29 , C12N15/82
CPC分类号： C12N15/8279
摘要： Transcription factor polynucleotides and polypeptides incorporated into expression vectors have been introduced into plants and were ectopically expressed. Transgenic plants transformed with many of these expression vectors have been shown to be more resistant to disease, and in some cases, to more than one type of pathogen.
摘要翻译：掺入表达载体的转录因子多核苷酸和多肽已被引入植物中并被异位表达。已经显示用许多这些表达载体转化的转基因植物对疾病更有抗性，在某些情况下，对多种类型的病原体更有抗性。

3. 发明申请

WO2007028165A2 STRESS TOLERANCE IN PLANTS 审中-公开
标题翻译：植物耐应力
公开(公告)号：WO2007028165A2
公开(公告)日：2007-03-08
申请号：PCT/US2006/034615
申请日：2006-08-31
申请人： MENDEL BIOTECHNOLOGY, INC. , GUTTERSON, Neal, I , RATCLIFFE, Oliver, J. , REUBER, Lynne, T. , CENTURY, Karen, S. , KROLIKOWSKI, Katherine , COSTA, Jennifer, M. , CREELMAN, Robert, A. , HEMPEL, Frederick, D. , KUMIMOTO, Roderick, W. , QUEEN, Emily, L. , REPETTI, Peter , ADAM, Luc
发明人： GUTTERSON, Neal, I , RATCLIFFE, Oliver, J. , REUBER, Lynne, T. , CENTURY, Karen, S. , KROLIKOWSKI, Katherine , COSTA, Jennifer, M. , CREELMAN, Robert, A. , HEMPEL, Frederick, D. , KUMIMOTO, Roderick, W. , QUEEN, Emily, L. , REPETTI, Peter , ADAM, Luc
IPC分类号： A01H1/00 , C12N15/82 , C12N5/04
CPC分类号： C07K14/415 , C12N15/8261 , C12N15/8273 , Y02A40/146
摘要： Transcription factor polynucleotides and polypeptides incorporated into expression vectors have been introduced into plants and were ectopically expressed. Transgenic plants transformed with many of these expression vectors have been shown to be more resistant to disease (in some cases, to more than one pathogen), or more tolerant to an abiotic stress (in some cases, to more than one abiotic stress). The abiotic stress may include salt, hyperosmotic stress, heat, cold, drought, or low nitrogen conditions.
摘要翻译：掺入表达载体的转录因子多核苷酸和多肽已被引入植物中并被异位表达。已经显示用许多这些表达载体转化的转基因植物对疾病（在一些情况下，多于一种病原体）更耐受或更耐受非生物胁迫（在某些情况下，对多于一种非生物胁迫）。非生物胁迫可能包括盐，高渗胁迫，热，冷，干旱或低氮条件。

4. 发明申请

WO2007028165A8 STRESS TOLERANCE IN PLANTS 审中-公开
标题翻译：植物耐应力
公开(公告)号：WO2007028165A8
公开(公告)日：2008-04-03
申请号：PCT/US2006034615
申请日：2006-08-31
申请人： MENDEL BIOTECHNOLOGY INC , GUTTERSON NEAL I , RATCLIFFE OLIVER J , REUBER T LYNNE , CENTURY KAREN S , KROLIKOWSKI KATHERINE , COSTA JENNIFER M , CREELMAN ROBERT A , HEMPEL FREDERICK D , KUMIMOTO RODERICK W , QUEEN EMILY L , REPETTI PETER , ADAM LUC
发明人： GUTTERSON NEAL I , RATCLIFFE OLIVER J , REUBER T LYNNE , CENTURY KAREN S , KROLIKOWSKI KATHERINE , COSTA JENNIFER M , CREELMAN ROBERT A , HEMPEL FREDERICK D , KUMIMOTO RODERICK W , QUEEN EMILY L , REPETTI PETER , ADAM LUC
IPC分类号： A01H1/00 , C12N5/04 , C12N15/82
CPC分类号： C07K14/415 , C12N15/8261 , C12N15/8273 , Y02A40/146
摘要： Transcription factor polynucleotides and polypeptides incorporated into expression vectors have been introduced into plants and were ectopically expressed. Transgenic plants transformed with many of these expression vectors have been shown to be more resistant to disease (in some cases, to more than one pathogen), or more tolerant to an abiotic stress (in some cases, to more than one abiotic stress). The abiotic stress may include salt, hyperosmotic stress, heat, cold, drought, or low nitrogen conditions.
摘要翻译：掺入表达载体的转录因子多核苷酸和多肽已被引入植物中并被异位表达。已经显示用许多这些表达载体转化的转基因植物对疾病（在一些情况下，多于一种病原体）更耐受或更耐受非生物胁迫（在某些情况下，对多于一种非生物胁迫）。非生物胁迫可能包括盐，高渗胁迫，热，冷，干旱或低氮条件。

5. 发明申请

WO2007120820A2 PLANT DISEASE RESISTANCE GENES AND PROTEINS 审中-公开
标题翻译：植物抗病基因和蛋白质
公开(公告)号：WO2007120820A2
公开(公告)日：2007-10-25
申请号：PCT/US2007/009124
申请日：2007-04-12
申请人： MENDEL BIOTECHNOLOGY, INC. , REUBER, T., Lynne , CENTURY, Karen, S.
发明人： REUBER, T., Lynne , CENTURY, Karen, S.
IPC分类号： A01H5/00
CPC分类号： C12N15/8279
摘要： Transcription factor polynucleotides and polypeptides incorporated into expression vectors have been introduced into plants and were ectopically expressed. Transgenic plants transformed with many of these expression vectors have been shown to be more resistant to disease, and in some cases, to more than one type of pathogen.
摘要翻译：掺入表达载体的转录因子多核苷酸和多肽已被引入植物中并被异位表达。已经显示用许多这些表达载体转化的转基因植物对疾病更有抗性，在某些情况下，对多种类型的病原体更有抗性。

6. 发明申请

WO2005047516A3 PLANT TRANSCRIPTIONAL REGULATORS 审中-公开
标题翻译：植物转录调节因子
公开(公告)号：WO2005047516A3
公开(公告)日：2006-06-15
申请号：PCT/US2004037584
申请日：2004-11-12
申请人： MENDEL BIOTECHNOLOGY INC , HEARD JACQUELINE E , RIECHMANN JOSE LUIS , CREELMAN ROBERT A , RATCLIFFE OLIVER J , CANALES ROGER D , REPETTI PETER , KUMIMOTO RODERICK W , GUTTERSON NEAL I , REUBER T LYNNE , PINEDA OMAIRA , SHERMAN BRADLEY K , MORRISON TRACY A , KEDDIE JAMES S , JIANG CAI-ZHONG , CENTURY KAREN S , ADAM LUC , ZHANG JAMES Z , HEMPEL FREDERICK D , LIBBY JEFFREY M
发明人： HEARD JACQUELINE E , RIECHMANN JOSE LUIS , CREELMAN ROBERT A , RATCLIFFE OLIVER J , CANALES ROGER D , REPETTI PETER , KUMIMOTO RODERICK W , GUTTERSON NEAL I , REUBER T LYNNE , PINEDA OMAIRA , SHERMAN BRADLEY K , MORRISON TRACY A , KEDDIE JAMES S , JIANG CAI-ZHONG , CENTURY KAREN S , ADAM LUC , ZHANG JAMES Z , HEMPEL FREDERICK D , LIBBY JEFFREY M
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C07K14/415 , C12N15/8261 , Y02A40/146
摘要： The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having improved tolerance to drought, shade, and low nitrogen conditions, as compared to wild-type or reference plants.
摘要翻译：本发明涉及植物转录因子多肽，编码它们的多核苷酸，来自各种植物物种的同源物，以及使用多核苷酸和多肽产生对干旱，阴凉和低氮条件具有改善的耐受性的转基因植物的方法，与野生型或参照植物。

7. 发明申请

WO2006033708A3 BIOTIC AND ABIOTIC STRESS TOLERANCE IN PLANTS 审中-公开
公开(公告)号：WO2006033708A3
公开(公告)日：2006-03-30
申请号：PCT/US2005/027151
申请日：2005-07-29
申请人： MENDEL BIOTECHNOLOGY, INC , GUTTERSON, Neal, I. , RATCLIFFE, Oliver , QUEEN, Emily, L. , REUBER, Lynne, T. , CENTURY, Karen, S. , CANALES, Roger
发明人： GUTTERSON, Neal, I. , RATCLIFFE, Oliver , QUEEN, Emily, L. , REUBER, Lynne, T. , CENTURY, Karen, S. , CANALES, Roger
IPC分类号： C12N15/82 , A01H5/00
摘要： The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties, including resistance to disease and tolerance to low nitrogen, drought, and other abiotic stresses, as compared to wild-type or control plants.

8. 发明申请

WO2005047516A2 PLANT TRANSCRIPTIONAL REGULATORS 审中-公开
标题翻译：植物转录调节因子
公开(公告)号：WO2005047516A2
公开(公告)日：2005-05-26
申请号：PCT/US2004/037584
申请日：2004-11-12
申请人： MENDEL BIOTECHNOLOGY, INC. , HEARD, Jacqueline, E. , RIECHMANN, Jose Luis , CREELMAN, Robert, A. , RATCLIFFE, Oliver, J. , CANALES, Roger, D. , REPETTI, Peter , KUMIMOTO, Roderick, W. , GUTTERSON, Neal, I. , REUBER, T., Lynne , PINEDA, Omaira , SHERMAN, Bradley, K. , MORRISON, Tracy, A. , KEDDIE, James, S. , JIANG, Cai-Zhong , CENTURY, Karen, S. , ADAM, Luc , ZHANG, James, Z. , HEMPEL, Frederick, D. , LIBBY, Jeffrey, M.
发明人： HEARD, Jacqueline, E. , RIECHMANN, Jose Luis , CREELMAN, Robert, A. , RATCLIFFE, Oliver, J. , CANALES, Roger, D. , REPETTI, Peter , KUMIMOTO, Roderick, W. , GUTTERSON, Neal, I. , REUBER, T., Lynne , PINEDA, Omaira , SHERMAN, Bradley, K. , MORRISON, Tracy, A. , KEDDIE, James, S. , JIANG, Cai-Zhong , CENTURY, Karen, S. , ADAM, Luc , ZHANG, James, Z. , HEMPEL, Frederick, D. , LIBBY, Jeffrey, M.
IPC分类号： C12N15/82
CPC分类号： C07K14/415 , C12N15/8261 , Y02A40/146
摘要： The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having improved tolerance to drought, shade, and low nitrogen conditions, as compared to wild-type or reference plants.
摘要翻译：本发明涉及植物转录因子多肽，编码它们的多核苷酸，来自各种植物物种的同源物，以及使用多核苷酸和多肽产生对干旱，阴凉和低氮条件具有改善的耐受性的转基因植物的方法，与野生型或参考植物。

9. 发明申请

WO2007028165A3 STRESS TOLERANCE IN PLANTS 审中-公开
标题翻译：植物耐受的压力
公开(公告)号：WO2007028165A3
公开(公告)日：2008-09-12
申请号：PCT/US2006034615
申请日：2006-08-31
申请人： MENDEL BIOTECHNOLOGY INC , GUTTERSON NEAL I , RATCLIFFE OLIVER J , REUBER T LYNNE , CENTURY KAREN S , KROLIKOWSKI KATHERINE , COSTA JENNIFER M , CREELMAN ROBERT A , HEMPEL FREDERICK D , KUMIMOTO RODERICK W , QUEEN EMILY L , REPETTI PETER , ADAM LUC
发明人： GUTTERSON NEAL I , RATCLIFFE OLIVER J , REUBER T LYNNE , CENTURY KAREN S , KROLIKOWSKI KATHERINE , COSTA JENNIFER M , CREELMAN ROBERT A , HEMPEL FREDERICK D , KUMIMOTO RODERICK W , QUEEN EMILY L , REPETTI PETER , ADAM LUC
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C07K14/415 , C12N15/8261 , C12N15/8273 , Y02A40/146
摘要： Transcription factor polynucleotides and polypeptides incorporated into expression vectors have been introduced into plants and were ectopically expressed. Transgenic plants transformed with many of these expression vectors have been shown to be more resistant to disease (in some cases, to more than one pathogen), or more tolerant to an abiotic stress (in some cases, to more than one abiotic stress). The abiotic stress may include salt, hyperosmotic stress, heat, cold, drought, or low nitrogen conditions.
摘要翻译：掺入到表达载体中的转录因子多核苷酸和多肽已被引入植物中并被异位表达。已显示用许多这些表达载体转化的转基因植物对疾病（在一些情况下，对多于一种病原体）更耐抗性，或对非生物胁迫（在一些情况下对多于一种非生物胁迫）更耐受。非生物胁迫可能包括盐，高渗胁迫，热，冷，干旱或低氮条件。

10. 发明申请

WO2007127186A3 DISEASE-INDUCIBLE PROMOTERS 审中-公开
公开(公告)号：WO2007127186A3
公开(公告)日：2007-11-08
申请号：PCT/US2007/009890
申请日：2007-04-23
申请人： MENDEL BIOTECHNOLOGY, INC. , ADAM, Luc , REUBER, T., Lynne , CENTURY, Karen, S.
发明人： ADAM, Luc , REUBER, T., Lynne , CENTURY, Karen, S.
IPC分类号： C12N15/09 , C12N15/29 , C12N15/82 , A01H1/00 , A01H5/00 , A01H5/10
摘要： Disease-inducible promoter sequences have been identified that may be used to produce transgenic plants that are both more resistant to disease than control plants, and are wild-type or nearly wild type in appearance. Any of these disease-inducible promoters may be incorporated into expression vectors that each comprise a defense response protein operably linked to the promoter. The expression vectors can be introduced into plants and the defense response protein then ectopically expressed. Transgenic plants transformed with many of these expression vectors have been shown to be more resistant to disease, in some cases, to more than one type of pathogen, and yet are similar to wild type plants in their morphology and development.

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