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    • 7. 发明申请
    • NANOPARTICLE PROBES WITH RAMAN SPECTROSCOPIC FINGERPRINTS FOR ANALYTE DETECTION
    • 用于分析物检测的拉曼光谱指纹图谱的纳米颗粒探针
    • WO03095973A2
    • 2003-11-20
    • PCT/US0314100
    • 2003-05-07
    • UNIV NORTHWESTERNMIRKIN CHADCAO YUN-WEIJIN RONGCHAO
    • MIRKIN CHADCAO YUN-WEIJIN RONGCHAO
    • G01N21/65C12M1/00C12N15/09C12Q1/02C12Q1/68G01N33/53G01N33/543G01N33/553G01N33/566G01N33/58G01N
    • G01N33/587C12Q1/6816G01N33/54373G01N33/583C12Q2565/632C12Q2565/519C12Q2537/125
    • The invention encompasses reagents comprising particles with at least one Raman dye and a specific binding members bound thereto and methods of using such reagents. The invention also encompasses reagents of a specific binding member and two or more different Raman dyes and methods for using such reagents. New types of particle probes having a specific binding member bound thereto are described. These reagents are used in a novel detection strategy that utilizes the catalytic properties of the Au nanoparticles to generate a silver coating that can behave as a surface-enhanced Raman scattering (SERS) promoter for the dye-labeled particles that have been captured by target and an underlying chip in microarray format. The strategy provides the high sensitivity and high selectivity attributes of grey-scale scanometric detection but provides a route to multiplexing and ratioing capabilities since a very large number of probes can be designed based upon the concept of using a Raman tag as a spectroscopic fingerprint in detection. These spectra are used as fingerprints to differentiate oligonucleotide or other targets in one solution. This method has been used to distinguish six dissimilar DNA targets with six Raman labeled nanoparticle probes, and also two RNA targets with single nucleotide polymorphisms (SNPs).
    • 本发明包括含有至少一种拉曼染料和与其结合的特异性结合成员的颗粒的试剂和使用这些试剂的方法。 本发明还包括特异性结合成员和两种或更多种不同拉曼染料的试剂和使用这些试剂的方法。 描述具有与其结合的特异性结合成员的新型粒子探针。 这些试剂用于一种新的检测策略,其利用Au纳米粒子的催化性能产生可以作为被目标捕获的染料标记的颗粒的表面增强拉曼散射(SERS)启动子的银涂层, 微芯片格式的底层芯片。 该策略提供了灰度扫描测量检测的高灵敏度和高选择性属性,但是提供了多路复用和比较能力的途径,因为可以基于在检测中使用拉曼标签作为光谱指纹的概念来设计非常大量的探针 。 这些光谱用作指纹以在一种溶液中区分寡核苷酸或其他靶标。 该方法已被用于区分六个不同的DNA靶标与六个拉曼标记的纳米粒子探针,以及两个具有单核苷酸多态性(SNPs)的RNA靶标。