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1. 发明申请

US20090075828A1 INTEGRATED PROTEIN CHIP ASSAY 审中-公开
标题翻译：一体化蛋白质芯片测定
公开(公告)号：US20090075828A1
公开(公告)日：2009-03-19
申请号：US12212344
申请日：2008-09-17
申请人： Anna Astriab Fisher , Bryce P. Nelson
发明人： Anna Astriab Fisher , Bryce P. Nelson
IPC分类号： C40B30/00 , C40B40/10 , C40B60/12
CPC分类号： C40B30/04 , G01N33/6845
摘要： The present invention relates to novel methodologies for performing multiplexed assays with high precision and sensitivity. In particular, the present invention relates to improving assay sensitivity and precision by combining the normalization of multiplexed assay data using an internal standard with scattered application of samples and standards replicates throughout sample wells on a slide or set of slides as well as scattered replicates of arrayed probes in a single well. These compositions and methods can be used to perform multiplexed assays for analytes in patient and other test samples. In particular, these methods have applications for Quantitative Multi-analyte Immunoassays (QMI) to measure proteins in human serum and plasma.
摘要翻译：本发明涉及用于以高精度和灵敏度进行多重测定的新方法。特别地，本发明涉及通过使用内部标准的多重测定数据的归一化与样品的分散施用和标准样品复合在载玻片或幻灯片上的样品孔中进行组合以及排列的分散复制来提高测定灵敏度和精度在一个井中探测。这些组合物和方法可用于对患者和其他测试样品中的分析物进行多重测定。特别地，这些方法适用于定量多分析物免疫测定（QMI）来测量人血清和血浆中的蛋白质。

2. 发明授权

US08470590B2 Multiwell plate device 有权
标题翻译：多孔板装置
公开(公告)号：US08470590B2
公开(公告)日：2013-06-25
申请号：US12120591
申请日：2008-05-14
申请人： Chris Adrien , John C. Bart , James Clements , David Moore , Bryce P. Nelson
发明人： Chris Adrien , John C. Bart , James Clements , David Moore , Bryce P. Nelson
IPC分类号： C12M1/22
CPC分类号： B01L3/5085 , B01L3/50855 , B01L9/523 , B01L2300/0636 , B01L2300/0829
摘要： A multiwell plate device is provided having a frame, a substantially flat substrate and a multiwell structure supported by the substrate. The multiwell structure includes multiple bottomless wells formed therein. The substrate is supported by the frame and may be processed by an automated arrayer or instrument that is used to print or spot arrays in a pattern on a reaction surface of the substrate. Thereafter, the multiwell structure may be engaged with the substrate and the multiwell structure and substrate may be engaged with the frame in an upright orientation. For scanning or other analysis, the multiwell structure and substrate may be disengaged from the frame, inverted 180°, and then reengaged with the frame in the inverted orientation.
摘要翻译：提供了一种多孔板装置，其具有框架，基本平坦的基板和由基板支撑的多孔结构。多井结构包括在其中形成的多个无底孔。基板由框架支撑，并且可以由用于在基板的反应表面上以图案打印或点阵的自动排列器或仪器来处理。此后，多孔结构可以与基底接合，并且多孔结构和基底可以以直立的方向与框架接合。对于扫描或其他分析，多孔结构和衬底可以与框架脱离180°，然后以反方向重新接合框架。

3. 发明申请

US20090253586A1 SUBSTRATES FOR MULTIPLEXED ASSAYS AND USES THEREOF 审中-公开
标题翻译：用于多重测定的基质及其用途
公开(公告)号：US20090253586A1
公开(公告)日：2009-10-08
申请号：US12390074
申请日：2009-02-20
申请人： Bryce P. Nelson , John C. Bart , Bradley H. Garcia
发明人： Bryce P. Nelson , John C. Bart , Bradley H. Garcia
IPC分类号： C40B30/04 , C40B40/00 , C40B40/08 , C40B40/10 , C40B60/12
CPC分类号： G01N33/543 , G01N21/6452 , G01N33/54306 , G01N33/54393 , G01N2021/6441
摘要： The present invention relates to novel methodologies for performing multiplexed assays for biological molecules such as proteins and nucleic acids. In particular, the present invention provides multiplexed assays using precipitating reagents and optically clear nitrocellulose-coated solid supports.
摘要翻译：本发明涉及用于对生物分子例如蛋白质和核酸进行多重测定的新方法。特别地，本发明提供使用沉淀试剂和光学透明的硝化纤维素包被的固体支持物的多重测定。

4. 发明授权

US06849397B2 Label-free detection of nucleic acids via surface plasmon resonance 失效
标题翻译：通过表面等离子体共振无标记检测核酸
公开(公告)号：US06849397B2
公开(公告)日：2005-02-01
申请号：US09998551
申请日：2001-11-29
申请人： Bryce P. Nelson , Mark R. Liles , Kendra Frederick , Robert M. Corn , Robert M. Goodman
发明人： Bryce P. Nelson , Mark R. Liles , Kendra Frederick , Robert M. Corn , Robert M. Goodman
IPC分类号： C07H21/04 , C12M1/34 , C12P13/14 , C12Q1/68 , C12Q1/70 , G01J4/00 , G01N20060101 , G01N33/48 , G01N33/50 , G06F19/00
CPC分类号： B82Y30/00 , B82Y15/00 , C12Q1/6837 , C12Q2565/628
摘要： Disclosed is a method to detect unlabeled nucleic acids (DNA and/or RNA) in a taxa, species, and organelle-specific fashion using surface plasmon resonance (SPR) imaging. Taxa-specific, species-specific, or organelle-specific nucleic acids are affixed to an SPR-suitable substrate. A nucleic acid sample to be analyzed is then contacted with the SPR-substrate and the substrate analyzed to determine the presence or absence of specific hybridization between the nucleic acids bound to the substrate and the nucleic acids contained in the sample. The method does not require that either the bound nucleic acids nor the sample nucleic acids be labeled. The method can be used to identify the source of nucleic acids, their sequence, as well as to identify organisms and place them within a given taxonomic hierarchy.
摘要翻译：公开了一种使用表面等离子体共振（SPR）成像检测分类群，物种和细胞器特异性方式中的未标记核酸（DNA和/或RNA）的方法。分子标记特异性，物种特异性或细胞器特异性核酸固定在适合SPR的底物上。待分析的核酸样品然后与SPR-底物接触并分析底物，以确定与底物结合的核酸与样品中所含核酸之间是否存在特异性杂交。该方法不要求结合的核酸和样品核酸都被标记。该方法可用于鉴定核酸的来源，其序列，以及鉴定生物体并将其置于给定的分类层级中。

5. 发明申请

US20080287307A1 MULTIWELL PLATE DEVICE 有权
标题翻译：多层板设备
公开(公告)号：US20080287307A1
公开(公告)日：2008-11-20
申请号：US12120591
申请日：2008-05-14
申请人： Chris Adrien , John C. Bart , James Clements , David Moore , Bryce P. Nelson
发明人： Chris Adrien , John C. Bart , James Clements , David Moore , Bryce P. Nelson
IPC分类号： C40B30/00 , C40B60/12
CPC分类号： B01L3/5085 , B01L3/50855 , B01L9/523 , B01L2300/0636 , B01L2300/0829
摘要： A multiwell plate device is provided having a frame, a substantially flat substrate and a multiwell structure supported by the substrate. The multiwell structure includes multiple bottomless wells formed therein. The substrate is supported by the frame and may be processed by an automated arrayer or instrument that is used to print or spot arrays in a pattern on a reaction surface of the substrate. Thereafter, the multiwell structure may be engaged with the substrate and the multiwell structure and substrate may be engaged with the frame in an upright orientation. For scanning or other analysis, the multiwell structure and substrate may be disengaged from the frame, inverted 180°, and then reengaged with the frame in the inverted orientation.
摘要翻译：提供了一种多孔板装置，其具有框架，基本平坦的基板和由基板支撑的多孔结构。多井结构包括在其中形成的多个无底孔。基板由框架支撑，并且可以由用于在基板的反应表面上以图案打印或点阵的自动排列器或仪器来处理。此后，多孔结构可以与基底接合，并且多孔结构和基底可以以直立的方向与框架接合。对于扫描或其他分析，多孔结构和衬底可以与框架脱离180°，然后以反方向重新接合框架。

6. 发明授权

US06878523B2 Molecular interaction assays on a solid surface 失效
标题翻译：固体表面上的分子相互作用测定
公开(公告)号：US06878523B2
公开(公告)日：2005-04-12
申请号：US10141611
申请日：2002-05-08
申请人： Bryce P. Nelson , Todd C. Strother
发明人： Bryce P. Nelson , Todd C. Strother
IPC分类号： C12M1/34 , C12Q1/68 , C40B30/04 , G01N33/53 , G01N33/68
CPC分类号： C40B30/04 , B82Y30/00 , G01N33/6845
摘要： The present invention relates to novel methods for the analysis of molecular interactions. In particular, the present invention relates to compositions and methods for molecular interaction assays performed in solution and on solid surfaces. The present invention thus provides improves methods and compositions for the analysis of molecular interactions.
摘要翻译：本发明涉及分子相互作用分析的新方法。特别地，本发明涉及在溶液和固体表面上进行的分子相互作用测定的组合物和方法。因此，本发明提供改进用于分析相互作用分析的方法和组合物。

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