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1. 发明授权

US07745558B2 Entrapped β-cyclodextrin polymer and a method of preparing the same 有权
标题翻译：包埋的＆amp;环糊精聚合物及其制备方法
公开(公告)号：US07745558B2
公开(公告)日：2010-06-29
申请号：US11947035
申请日：2007-11-29
申请人： Man Ho Choi , Dong Hyun Kim , Bong Chul Chung , Ju-Yeon Moon
发明人： Man Ho Choi , Dong Hyun Kim , Bong Chul Chung , Ju-Yeon Moon
IPC分类号： C08F251/00
CPC分类号： C08F251/00 , C08B37/0012 , C08L5/16 , C08L51/02 , G01N33/548 , G01N33/743 , G01N2600/00 , C08L2666/02
摘要： Provided are entrapped β-cyclodextrin polymers and a method of preparing the same. Particularly, entrapped β-cyclodextrin polymers are prepared by adding epichlorohydrin to β-cyclodextrin to prepare a polymer in a gel state, entrapping the polymer by entrapment of metal ions on the surface of the polymer and pulverizing the entrapped polymer to prepare powders. According to the entrapped β-cyclodextrin polymers, steroid hormones contained in a biological sample can be simply extracted without using an additional device required in conventional solid-phase extraction.
摘要翻译：提供的是包埋的＆amp;环糊精聚合物及其制备方法。特别地，通过将环氧氯丙烷加入到环糊精中以制备凝胶状态的聚合物来制备包埋的＆amp;环糊精聚合物，通过在聚合物的表面上截留金属离子并粉碎被捕获的聚合物以制备粉末而将聚合物截留。根据捕获的环糊精聚合物，可以简单地提取生物样品中所含的类固醇激素，而无需使用常规固相萃取所需的附加装置。

2. 发明申请

US20110207230A1 EVALUATION OF 24S-HYDROXYCHOLESTEROL IN HAIR FOR METABOLIC BIOMARKER OF ALZHEIMER'S DISEASE 审中-公开
标题翻译：阿尔茨海默病代谢生物标记物中的24S-羟基胆甾醇的评价
公开(公告)号：US20110207230A1
公开(公告)日：2011-08-25
申请号：US12712574
申请日：2010-02-25
申请人： Bong Chul CHUNG , Man Ho Choi , Kyung Mi Kim
发明人： Bong Chul CHUNG , Man Ho Choi , Kyung Mi Kim
IPC分类号： G01N33/92
CPC分类号： G01N33/92 , G01N33/4833 , G01N33/6848 , G01N33/6896 , G01N2800/2821 , G01N2800/50
摘要： Disclosed is a method for detection of 24S-hydroxycholesterol in hair. More specifically, a trace amount of hair is hydrolyzed under basic condition and metabolites including 24S-hydroxycholesterol are purified and extracted by solid-phase extraction (SPE). Then, after derivatization of the resulting extract with trimethylsilyl (TMS), 24S-hydroxycholesterol is detected by gas chromatography-mass spectrometry (GC-MS).
摘要翻译：公开了一种用于检测头发中24S-羟基胆固醇的方法。更具体地说，在碱性条件下水解微量的毛发，通过固相萃取（SPE）纯化和提取包括24S-羟基胆固醇的代谢物。然后，用三甲基甲硅烷基（TMS）衍生得到的提取物后，通过气相色谱 - 质谱（GC-MS）检测24S-羟基胆固醇。

3. 发明授权

US07951890B2 Solid-phase extraction method of steroid hormones by entrapped β-cyclodextrin polymers 有权
标题翻译：甾体激素的固相萃取法通过包埋和环糊精聚合物
公开(公告)号：US07951890B2
公开(公告)日：2011-05-31
申请号：US11947004
申请日：2007-11-29
申请人： Man Ho Choi , Dong Hyun Kim , Bong Chul Chung , Ju-Yeon Moon
发明人： Man Ho Choi , Dong Hyun Kim , Bong Chul Chung , Ju-Yeon Moon
IPC分类号： C08F251/00
CPC分类号： C07J75/00
摘要： Provided is a method for selective extraction of steroid compounds using entrapped β-cyclodextrin polymers. Particularly, steroid hormones can be effectively selected from a biological sample by selectively extracting steroid compounds using entrapped β-cyclodextrin polymers, prepared by adding epichlorohydrin to β-cyclodextrin to prepare a polymer in a gel state, entrapping the polymer and pulverizing the products without using an additional device required in conventional solid-phase extraction.
摘要翻译：提供了一种使用包埋的＆amp;环糊精聚合物选择性提取类固醇化合物的方法。特别地，可以通过选择性地提取类固醇化合物来选择性地从生物样品中选择类固醇激素，该捕获的＆amp;环糊精聚合物通过将环氧氯丙烷加入到＆amp;环糊精中制备凝胶状态的聚合物，捕获聚合物和粉碎产物而不使用常规固相萃取所需的附加装置。

4. 发明授权

US07678579B2 Method for analysis metabolite difference between biological samples 失效
标题翻译：分析生物样品代谢物差异的方法
公开(公告)号：US07678579B2
公开(公告)日：2010-03-16
申请号：US11784292
申请日：2007-04-05
申请人： Byung Hwa Jung , Bong Chul Chung
发明人： Byung Hwa Jung , Bong Chul Chung
IPC分类号： G01N30/02
CPC分类号： G01N33/6848 , G01N30/7206 , G06F19/24 , Y10T436/255
摘要： Disclosed is a method of extracting all metabolites existing in a biological sample to detect a comprehensive difference between metabolites of a control group and a test group, thereby verifying significance thereof. More particularly, the invention comprises a first step of analyzing a fraction extracted with a solid phase extraction method, a fraction obtained by extracting the remnant fraction with a liquid-liquid extraction method and fractions extracted with the liquid-liquid extraction method at two pH different from each other after the hydrolysis, with a gas chromatography-mass spectrometry; a second step of converting the chromatogram result into numerical values capable of being statistically processed; and a third step of analyzing the numerical values with a principal component analysis (PCA) and a discriminant analysis (DA) to detect a difference between the control group and the test group. According to the invention, the difference between the two groups can be comprehensively detected with the areas of the peaks on the chromatogram, without a standard material or verified quantitative method. Therefore, it is possible to detect the metabolic change in the organism due to the disease or gene mutation, without an accurate quantitative analysis of the metabolites.
摘要翻译：公开了提取生物样品中存在的所有代谢物的方法，以检测对照组和试验组的代谢物之间的综合差异，从而验证其意义。更具体地说，本发明包括分析用固相萃取法萃取的馏分的第一步骤，用液 - 液萃取方法提取剩余馏分获得的馏分，并用液 - 液萃取方法在两种pH值下提取馏分水解后，用气相色谱 - 质谱联用; 将色谱图结果转换为能够进行统计处理的数值的第二步骤; 以及通过主成分分析（PCA）和判别式分析（DA）分析数值以检测对照组和测试组之间的差异的第三步骤。根据本发明，可以用色谱图上峰的面积全面检测两组之间的差异，无需标准材料或经过验证的定量方法。因此，可以检测由于疾病或基因突变而导致的生物体的代谢变化，而不对该代谢产物进行准确的定量分析。

5. 发明申请

US20110071094A1 (D)-ALLOSE INDUCING PROGRAMMED CELL DEATH IN HORMONE REFRACTORY PROSTATE CANCER LINES 审中-公开
标题翻译：（D）-ALLOSE诱导编程性细胞死亡霍尔姆森耐克癌前列腺癌细胞系
公开(公告)号：US20110071094A1
公开(公告)日：2011-03-24
申请号：US12911279
申请日：2010-10-25
申请人： MYEONG OK KIM , BONG CHUL CHUNG
发明人： MYEONG OK KIM , BONG CHUL CHUNG
IPC分类号： A61K31/7004 , C07H3/02 , C12N5/09 , A61P35/00
CPC分类号： A61K31/70
摘要： The present disclosure relates allose inducing programmed cell death in hormone refractory prostate cancer cell lines. More particularly, the present disclosure relates to a D-allose inducing programmed cell death in hormone refractory prostate cancer cell line of DU145 in dose-dependent manner by repressing cell growth and inhibiting cell cycle as well as by reducing expression of caspase-3 and poly (ADP-ribose) polymerase (PARP).
摘要翻译：本公开内容涉及激素难治性前列腺癌细胞系中的allose诱导程序性细胞死亡。更具体地说，本公开内容涉及通过抑制细胞生长和抑制细胞周期以及通过降低半胱天冬酶-3和聚（ADP-核糖）聚合酶（PARP）。

6. 发明申请

US20080207931A1 Solid-Phase Extraction Method of Steroid Hormones by Entrapped Beta-Cyclodextrin Polymers 有权
标题翻译：固相β-环糊精聚合物类固醇激素的固相萃取方法
公开(公告)号：US20080207931A1
公开(公告)日：2008-08-28
申请号：US11947004
申请日：2007-11-29
申请人： Man Ho Choi , Dong Hyun Kim , Bong Chul Chung , Ju-Yeon Moon
发明人： Man Ho Choi , Dong Hyun Kim , Bong Chul Chung , Ju-Yeon Moon
IPC分类号： C07J75/00
CPC分类号： C07J75/00
摘要： Provided is a method for selective extraction of steroid compounds using entrapped β-cyclodextrin polymers. Particularly, steroid hormones can be effectively selected from a biological sample by selectively extracting steroid compounds using entrapped β-cyclodextrin polymers, prepared by adding epichlorohydrin to β-cyclodextrin to prepare a polymer in a gel state, entrapping the polymer and pulverizing the products without using an additional device required in conventional solid-phase extraction.
摘要翻译：提供了一种使用夹带的β-环糊精聚合物选择性提取类固醇化合物的方法。特别地，可以通过使用夹带的β-环糊精聚合物选择性提取类固醇激素来有效地选择类固醇激素，所述β-环糊精聚合物通过将表氯醇加入到β-环糊精中以制备凝胶状态的聚合物，捕获聚合物并粉碎产物而不使用常规固相萃取所需的附加装置。

7. 发明申请

US20080076181A1 Method for analysis of metabolite difference between two biological samples using gas chromatography-mass spectrometry 失效
标题翻译：使用气相色谱 - 质谱法分析两种生物样品之间代谢物差异的方法
公开(公告)号：US20080076181A1
公开(公告)日：2008-03-27
申请号：US11784292
申请日：2007-04-05
申请人： Byung Hwa Jung , Bong Chul Chung
发明人： Byung Hwa Jung , Bong Chul Chung
IPC分类号： G01N30/02 , G01N1/28
CPC分类号： G01N33/6848 , G01N30/7206 , G06F19/24 , Y10T436/255
摘要： Disclosed is a method of extracting all metabolites existing in a biological sample to detect a comprehensive difference between metabolites of a control group and a test group, thereby verifying significance thereof. More particularly, the invention comprises a first step of analyzing a fraction extracted with a solid phase extraction method, a fraction obtained by extracting the remnant fraction with a liquid-liquid extraction method and fractions extracted with the liquid-liquid extraction method at two pH different from each other after the hydrolysis, with a gas chromatography-mass spectrometry; a second step of converting the chromatogram result into numerical values capable of being statistically processed; and a third step of analyzing the numerical values with a principal component analysis (PCA) and a discriminant analysis (DA) to detect a difference between the control group and the test group. According to the invention, the difference between the two groups can be comprehensively detected with the areas of the peaks on the chromatogram, without a standard material or verified quantitative method. Therefore, it is possible to detect the metabolic change in the organism due to the disease or gene mutation, without an accurate quantitative analysis of the metabolites.
摘要翻译：公开了提取生物样品中存在的所有代谢物的方法，以检测对照组和试验组的代谢物之间的综合差异，从而验证其意义。更具体地说，本发明包括分析用固相萃取法萃取的馏分的第一步骤，用液 - 液萃取方法提取剩余馏分获得的馏分，并用液 - 液萃取方法在两种pH值下提取馏分水解后，用气相色谱 - 质谱联用; 将色谱图结果转换为能够进行统计处理的数值的第二步骤; 以及通过主成分分析（PCA）和判别式分析（DA）分析数值以检测对照组和测试组之间的差异的第三步骤。根据本发明，可以用色谱图上峰的面积全面检测两组之间的差异，无需标准材料或经过验证的定量方法。因此，可以检测由于疾病或基因突变而导致的生物体的代谢变化，而不对该代谢产物进行准确的定量分析。

8. 发明申请

US20080194784A1 Entrapped Beta-Cyclodextrin Polymer and a Method of Preparing the Same 有权
标题翻译：包封的β-环糊精聚合物及其制备方法
公开(公告)号：US20080194784A1
公开(公告)日：2008-08-14
申请号：US11947035
申请日：2007-11-29
申请人： Man Ho Choi , Dong Hyun Kim , Bong Chul Chung , Ju-Yeon Moon
发明人： Man Ho Choi , Dong Hyun Kim , Bong Chul Chung , Ju-Yeon Moon
IPC分类号： C08F251/00
CPC分类号： C08F251/00 , C08B37/0012 , C08L5/16 , C08L51/02 , G01N33/548 , G01N33/743 , G01N2600/00 , C08L2666/02
摘要： Provided are entrapped β-cyclodextrin polymers and a method of preparing the same. Particularly, entrapped β-cyclodextrin polymers are prepared by adding epichlorohydrin to β-cyclodextrin to prepare a polymer in a gel state, entrapping the polymer by entrapment of metal ions on the surface of the polymer and pulverizing the entrapped polymer to prepare powders. According to the entrapped β-cyclodextrin polymers, steroid hormones contained in a biological sample can be simply extracted without using an additional device required in conventional solid-phase extraction.
摘要翻译：所提供的包封的β-环糊精聚合物及其制备方法。特别地，通过将表氯醇加入到β-环糊精中以制备凝胶状态的聚合物来制备包埋的β-环糊精聚合物，通过在聚合物的表面上捕获金属离子并粉碎被捕获的聚合物以制备粉末而将聚合物截留。根据捕获的β-环糊精聚合物，可以简单地提取生物样品中所含的类固醇激素，而不需要常规固相萃取所需的附加装置。

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