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1. 发明申请

US20070141594A1 Method of producing short hairpin library 审中-公开
标题翻译：短发夹文库的生产方法
公开(公告)号：US20070141594A1
公开(公告)日：2007-06-21
申请号：US11546019
申请日：2006-10-11
申请人： Biao Luo , David Root , Xiaoping Yang , Gregory Hinkle
发明人： Biao Luo , David Root , Xiaoping Yang , Gregory Hinkle
IPC分类号： C40B40/08 , C40B50/06
CPC分类号： C12N15/1093 , C12N15/111 , C12N2310/14 , C12N2310/53 , C12N2330/31
摘要： Described herein is a method of cloning synthetic oligos (including in situ synthesized oligos) into an (one or more) expression vector for library (e.g., shRNA library) production. The oligos are synthesized with one portion of the first stem of the hairpin, followed by a first loop sequence, the complete second stem, a second loop sequence, and finished with the remaining portion of the first stem of the hairpin. The two portions of the first stem anneal to the second stem, juxtaposing the 5′ end close to the 3′ end of the oligo. The methods described herein selected for hairpins with perfectly base-paired stems. After annealing, a ligase is added to the annealed oligos and the base-paired hairpins are preferentially annealed, and ligated, creating closed circular oligos. The now circularized hairpins served as templates for rolling circle amplification using a polymerase with high processivity. One or more primers complementary to the two strands of the amplified double stranded circular hairpins initiate the rolling circle amplification in the presence of a polymerase. Using primers (e.g., a sense and antisense primer), the rolling circle amplification yields double stranded hairpin sequences. These can be digested (e.g., using restriction enzymes) to produce a double-stranded hairpin fragment encoding a single hairpin. The fragment can be cloned into an appropriately digested vector for a variety of uses including expression.
摘要翻译：本文描述了将合成寡核苷酸（包括原位合成的寡核苷酸）克隆到（一个或多个）用于文库（例如shRNA文库）生产的表达载体中的方法。寡核苷酸与发夹的第一茎的一部分合成，随后是第一环序列，完整的第二茎，第二环序列，并用发夹的第一茎的剩余部分完成。第一茎的两个部分与第二茎退火，将5'末端与寡核苷酸的3'末端并列。本文所述的方法选择用于具有完全碱基配对茎的发夹。退火后，将连接酶加入到退火的寡核苷酸中，并且将碱基配对的发夹优先退火并连接，产生闭合的环状寡核苷酸。现在的圆形发夹作为使用高活性的聚合酶进行滚环扩增的模板。与扩增的双链环状发夹的两条链互补的一个或多个引物在聚合酶存在下引发滚环扩增。使用引物（例如有义和反义引物），滚环扩增产生双链发夹序列。这些可以被消化（例如，使用限制酶）以产生编码单个发夹的双链发夹片段。可以将片段克隆到适当消化的载体中，用于多种用途，包括表达。

2. 发明申请

US20110244556A1 Process For Preparing Ganoderma Spore Oil 审中-公开
标题翻译：制备灵芝孢子油的方法
公开(公告)号：US20110244556A1
公开(公告)日：2011-10-06
申请号：US12302026
申请日：2007-05-24
申请人： Yizheng Xie , Shenzhu Li , Burton B. Yang , Chong Li , Zhi Zhang , Qingping Wu , Guanzhou Chen , Biao Luo
发明人： Yizheng Xie , Shenzhu Li , Burton B. Yang , Chong Li , Zhi Zhang , Qingping Wu , Guanzhou Chen , Biao Luo
IPC分类号： C11C1/00
CPC分类号： A61K36/074 , A23V2002/00 , C11B1/025 , C11B1/104 , Y02P20/544 , A23V2200/324 , A23V2200/326
摘要： The present invention relates to a method for preparing Ganoderma spore oil which is in the field of biotechnology. The method includes using Ganoderma spore powder and Ganoderma powder (obtained from grinding fruiting bodies) as raw materials, applying enzymatic Ganoderma sporoderm broken methods, one-step granulation, supercritical CO2 extraction method, as well as centrifugation and refining. The light yellow oleaginous substances obtained posses a variety of physiological functions such as strengthening immunity, protecting the liver and inhibiting tumor cell growth, etc. Ganoderma spore oil prepared with the present technology contains not only spore extracts, but also extracts from Ganoderma fruiting bodies and mycelium by CO2 supercritical extraction, with more types of triterpenoids. The inhibitory effects of Ganoderma spore oil is a onefold higher than spore oil prepared from the physical preparation of the sporoderm-broken spore. Meanwhile, the problem of spore oil spoilage arising from oxidation is solved due to the low peroxide value within Ganoderma spore oil, and thereby the quality of Ganoderma spore oil is greatly improved.
摘要翻译：本发明涉及一种在生物技术领域中制备灵芝孢子油的方法。该方法包括使用灵芝孢子粉和灵芝粉（以研磨子实体得到）作为原料，应用灵芝孢子破碎法，一步制粒法，超临界CO2萃取法，离心精制法。所获得的淡黄色油性物质具有增强免疫力，保护肝脏和抑制肿瘤细胞生长等各种生理功能。本技术制备的灵芝孢子油不仅含有孢子提取物，还含有灵芝子实体的提取物，菌丝体通过二氧化碳超临界萃取，具有更多类型的三萜类化合物。灵芝孢子油的抑制作用比从孢子孢破碎孢子的物理制备制备的孢子油高出一倍。同时，由于灵芝孢子油中的过氧化值过低，解决了氧化引起的孢子油腐败问题，从而大大提高了灵芝孢子油的质量。

3. 发明授权

US07927105B2 Method and system for creating and delivering prescriptive learning 失效
标题翻译：创建和交付规范性学习的方法和系统
公开(公告)号：US07927105B2
公开(公告)日：2011-04-19
申请号：US10933608
申请日：2004-09-02
申请人： Charles W. Griffin , Marla A. Haddon , Biao Luo , John R. Malpass , Benjamin J. Montello
发明人： Charles W. Griffin , Marla A. Haddon , Biao Luo , John R. Malpass , Benjamin J. Montello
IPC分类号： G09B11/00 , G06F11/34
CPC分类号： G09B7/02 , G06Q10/0639 , G06Q10/06398
摘要： Method for prescriptive learning. The method includes providing a user independent hierarchy of competencies, competency characteristics and assessments with associated rules that identify learning activities and identifying a user specific path through the hierarchy for a selected competency and competency characteristic based on the hierarchy, scoring of at least one question associated with at least one assessment associated with the selected competency characteristic. The rules and user specific information provide a user specific identification of learning activities.
摘要翻译：规范性学习方法该方法包括提供用户独立的能力层级，能力特征和评估以及相关联的规则，所述关联规则识别学习活动，并且基于所述层次结构识别针对所选择的能力和能力特征的层次结构的用户特定路径，对至少一个问题相关联的评分至少有一个评估与所选择的能力特征相关联。规则和用户特定信息为用户提供了学习活动的特定标识。

4. 发明申请

US20060046237A1 Methods, systems and computer program products for creating and delivering prescriptive learning 失效
标题翻译：用于创建和提供规范性学习的方法，系统和计算机程序产品
公开(公告)号：US20060046237A1
公开(公告)日：2006-03-02
申请号：US10933608
申请日：2004-09-02
申请人： Charles Griffin , Maria Haddon , Biao Luo , John Malpass , Benjamin Montello
发明人： Charles Griffin , Maria Haddon , Biao Luo , John Malpass , Benjamin Montello
IPC分类号： G09B3/00 , G09B7/00
CPC分类号： G09B7/02 , G06Q10/0639 , G06Q10/06398
摘要： Methods, system and/or computer program products provide prescriptive learning by providing a user independent hierarchy of competencies, competency characteristics and assessments with associated rules that identify learning activities and identifying a user specific path through the hierarchy for a selected competency and competency characteristic based on the hierarchy, scoring of at least one question associated with at least one assessment associated with the selected competency characteristic, the rules and user specific information to provide a user specific identification of learning activities.
摘要翻译：方法，系统和/或计算机程序产品通过提供用户独立的能力，能力特征和评估层次来提供规范性学习，相关联的规则识别学习活动，并且基于所选择的能力和能力特征来识别通过层次结构的用户特定路径所述层次结构，与至少一个与所选择的能力特征相关联的评估相关联的至少一个问题的评分，所述规则和用户特定信息提供用户特定的学习活动识别。

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