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1. 发明专利

SK279805B6 DNA ENCODING 3-PHOSPHOGLYCERATE DEHYDROGENASE, EXPRESSION VECTORS AND CELLS FOR BIOSYNTHESIS OF SERINE AND SERINE-RELATED PRODUCTS 未知
公开(公告)号：SK279805B6
公开(公告)日：1999-04-13
申请号：SK70094
申请日：1992-08-17
申请人： BURLINGAME RICHARD P.
发明人： BURLINGAME RICHARD P
IPC分类号： C12N1/21 , C12N9/04 , C12N15/09 , C12N15/53 , C12N1/19 , C12P13/06 , C12P13/22 , C12R1/19 , C12R1/865
摘要： DNA encoding 3-phosphoglycerate dehydrogenase (PGD) with reduced sensitivity to inhibition by serine in comparison to wild-type PGD. The DNA encodes PGD which has at least some level of enzymatic activity useful for biosynthesis, and which retains that activity at higher serine levels than does the (unmodified) wild-type PGD. Also disclosed are: PGD having the amino acid sequence of the above- described engineered DNA; expression vectors comprising the engineered DNA and regulatory DNA positioned and oriented for expression of the engineered DNA in a host expression system; cells comprising such expression vectors; and methods for producing serine or a serine-derived product by culturing such cells.

2. 发明申请

WO2010019696A2 METHOD FOR PRODUCTION OF ISOPRENOIDS 审中-公开
标题翻译：异吲哚的生产方法
公开(公告)号：WO2010019696A2
公开(公告)日：2010-02-18
申请号：PCT/US2009/053589
申请日：2009-08-12
申请人： ALLYLIX, INC. , JULIEN, Bryan , BURLINGAME, Richard
发明人： JULIEN, Bryan , BURLINGAME, Richard
IPC分类号： C12Q1/68
CPC分类号： C12N9/1085 , C12N15/1079 , C12P5/002 , C12P5/007
摘要： The present invention is directed to variant squalene synthase enzymes, including Saccharomyces cerevisiae squalene synthase enzymes, and to nucleic acid molecules encoding these variant enzymes. These variant enzymes produce squalene at a lower rate than the wild-type enzyme, allowing more farnesyl pyrophosphate to be utilized for production of isoprenoid compounds, while still producing sufficient squalene to allow the S. cerevisiae cells to grow without the requirement for supplementation by sterols such as ergosterol. These variant enzymes, therefore, are highly suitable for the efficient production of isoprenoids.
摘要翻译：本发明涉及变体角鲨烯合酶，包括酿酒酵母角鲨烯合酶，以及编码这些变体酶的核酸分子。这些变体酶以比野生型酶低的速率产生角鲨烯，允许更多的法呢基焦磷酸盐用于生产类异戊二烯化合物，同时仍然产生足够的角鲨烯以允许酿酒酵母细胞生长而不需要由甾醇补充如麦角甾醇。因此，这些变体酶非常适合有效生产类异戊二烯。

3. 发明申请

WO2008008070A2 CONSTRUCTION OF HIGHLY EFFICIENT CELLULASE COMPOSITIONS FOR ENZYMATIC HYDROLYSIS OF CELLULOSE 审中-公开
标题翻译：高效纤维素组合物的构建用于纤维素的酶促水解
公开(公告)号：WO2008008070A2
公开(公告)日：2008-01-17
申请号：PCT/US2006027347
申请日：2006-10-06
申请人： DYADIC INTERNATIONAL INC , GUSAKOV ALEXANDER V , SALANOVICH TATYANA N , ANTONOV ALEXEY I , USTINOV BORIS B , OKUNEV OLEG N , BURLINGAME RICHARD , EMALFARB MARK , BAEZ MARCO , SINITSYN ARKADY P
发明人： GUSAKOV ALEXANDER V , SALANOVICH TATYANA N , ANTONOV ALEXEY I , USTINOV BORIS B , OKUNEV OLEG N , BURLINGAME RICHARD , EMALFARB MARK , BAEZ MARCO , SINITSYN ARKADY P
IPC分类号： C12P7/10
CPC分类号： C12N9/2437 , C12N9/244 , C12N9/2445 , C12P19/14 , C12Y302/01004 , C12Y302/01006 , C12Y302/01021 , C12Y302/01091 , Y02E50/16
摘要： This invention provides novel enzyme compositions using newly identified and isolated C. lucknowense enzymes, including CBH Ib, CBH IIb, EG II, EG VI, ß-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.
摘要翻译：本发明提供使用新鉴定和分离的C.幸运感酶的新型酶组合物，其包括CBH Ib，CBH IIb，EG II，EG VI，β-葡糖苷酶和木聚糖酶II与先前鉴定的酶CBH 1a，CBH IIa（前述）作为Endo 43）和EG V.这些酶组合物显示出将木质纤维素生物质（例如，通过有机溶剂预处理的Avicel，棉花，道格拉斯冷杉木材）转化为葡萄糖的极高的能力。两者具有纤维素结合模块（CBM）的CBH 1a和IIb显示出与来自棉花水解的相同真菌的三种主要内切葡聚糖酶（EG II，EG V，EG VI）的显着协同作用，以及与每种其他。酶组合物在木质纤维素生物质的水解中是有效的。

4. 发明申请

WO1993012235A1 MATERIALS AND METHODS FOR BIOSYNTHESIS OF SERINE AND SERINE-RELATED PRODUCTS 审中-公开
标题翻译：丝氨酸和丝氨酸相关产品的生物化学的材料和方法
公开(公告)号：WO1993012235A1
公开(公告)日：1993-06-24
申请号：PCT/EP1992001873
申请日：1992-08-17
申请人： WACKER-CHEMIE GMBH , BURLINGAME, Richard, P.
发明人： WACKER-CHEMIE GMBH
IPC分类号： C12N15/53
CPC分类号： C12N9/0006 , C12P13/06
摘要： DNA encoding 3-phosphoglycerate dehydrogenase (PGD) with reduced sensitivity to inhibition by serine in comparison to wild-type PGD - i.e., the DNA encodes PGD which has at least some level of enzymatic activity useful for biosynthesis, and which retains that activity at higher serine levels than does the (unmodified) wild-type PGD. Also disclosed are: a) PGD having the amino acid sequence of the above-described engineered DNA; b) expression vectors comprising the engineered DNA and regulatory DNA positioned and oriented for expression of the engineered DNA in a host expression system; c) cells comprising such expression vectors; and d) methods for producing serine or a serine-derived product by culturing such cells.
摘要翻译：与野生型PGD相比，编码3-磷酸甘油酸脱氢酶（PGD）的DNA具有降低的对丝氨酸抑制的敏感性，即DNA编码具有至少一些可用于生物合成的酶活性水平的PGD，并且其保留该活性在较高丝氨酸水平高于（未经修饰）野生型PGD。还公开了：a）具有上述工程DNA的氨基酸序列的PGD; b）包含工程化DNA和调节DNA的表达载体，其定位并定向以在宿主表达系统中表达工程化的DNA; c）包含这种表达载体的细胞; 和d）通过培养这些细胞产生丝氨酸或丝氨酸衍生产物的方法。

5. 发明申请

WO2010107303A3 CHRYSOSPORIUM LUCKNOWENSE PROTEIN PRODUCTION SYSTEM 审中-公开
标题翻译：木质素蛋白质蛋白质生产系统
公开(公告)号：WO2010107303A3
公开(公告)日：2010-12-16
申请号：PCT/NL2010000045
申请日：2010-03-16
申请人： DYADIC NEDERLAND B V , PUNT PETER J , BURLINGAME RICHARD PAUL , PYNNONEN CHRISTINE M , VERDOES JACOBA , OLSON PHILLIP T , WERY JAN , VISSER JOHANNES HEINRICH , EMALFARB MARK A , VISSER JACOB
发明人： PUNT PETER J , BURLINGAME RICHARD PAUL , PYNNONEN CHRISTINE M , OLSON PHILLIP T , WERY JAN , VISSER JOHANNES HEINRICH , EMALFARB MARK A , VISSER JACOB , VERDOES JAN CORMELIS
IPC分类号： C12N15/00 , C12N15/09 , C12P21/02 , C12R1/645
CPC分类号： C12N15/80 , C12N1/14 , C12N9/1205 , C12N9/2402 , C12N9/2428 , C12N9/2437 , C12N9/2442 , C12N9/248 , C12N15/01 , C12P21/02 , C12Q1/40 , C12R1/645 , C12Y302/01003 , C12Y302/01004 , C12Y302/01006 , C12Y302/01008 , C12Y302/01014 , C12Y302/01015 , C12Y302/01091 , C12Y304/21062 , C12Y304/23025 , G01N2333/93
摘要： The present invention provides a new fungal production system comprising a fungal host strain of Chrysosporium lucknowense wherein the endogenous cellulase secretion is less than 20 % of the endogenous cellulase secretion of Chrysosporium lucknowense strain UV 18-25. Preferably, also the secretion of endogenous protease, endogenous ß- glucanase and endogenous cellobiohydrolase is less than 20 % of the secretion of Chrysosporium lucknowense strain UV 18-25. Furthermore, fungal host strains are provided wherein several genes have been disrupted. According to another aspect of the invention a method for homologous and/or heterologous production of a pure protein with a purity of higher than 75 %, comprising expressing a gene encoding said protein in the strains according to the invention have been described. Furthermore, a method for production of artificial protein mixes comprising expressing a gene encoding each of said proteins in a strain according to the invention have been disclosed. Finally a method for simplified screening of strains functionally expressing a desired enzyme by application of said strains have been provided.
摘要翻译：本发明提供了一种新的真菌生产系统，其包含真菌宿主菌株Chrysosporium lucknowense，其中内源性纤维素酶分泌少于Chrysosporium lucknowense菌株UV18-25的内源性纤维素酶分泌的20％。优选地，内源性蛋白酶，内源性β-葡聚糖酶和内源性纤维二糖水解酶的分泌也小于Chrysosporium lucknowense菌株UV 18-25的分泌的20％。此外，提供真菌宿主菌株，其中几个基因已被破坏。根据本发明的另一方面，已经描述了用于纯化高于75％的纯蛋白质的同源和/或异源产生方法，其包括在本发明的菌株中表达编码所述蛋白质的基因。此外，已经公开了生产人造蛋白质混合物的方法，其包括在根据本发明的菌株中表达编码每种所述蛋白质的基因。已经提供了通过应用所述菌株来功能性表达所需酶的菌株的简化筛选的方法。

6. 发明申请

WO2010019696A3 METHOD FOR PRODUCTION OF ISOPRENOIDS 审中-公开
标题翻译：生产异丙二烯的方法
公开(公告)号：WO2010019696A3
公开(公告)日：2010-05-14
申请号：PCT/US2009053589
申请日：2009-08-12
申请人： ALLYLIX INC , JULIEN BRYAN , BURLINGAME RICHARD
发明人： JULIEN BRYAN , BURLINGAME RICHARD
IPC分类号： C12N1/00 , C12N1/19
CPC分类号： C12N9/1085 , C12N15/1079 , C12P5/002 , C12P5/007
摘要： The present invention is directed to variant squalene synthase enzymes, including Saccharomyces cerevisiae squalene synthase enzymes, and to nucleic acid molecules encoding these variant enzymes. These variant enzymes produce squalene at a lower rate than the wild-type enzyme, allowing more farnesyl pyrophosphate to be utilized for production of isoprenoid compounds, while still producing sufficient squalene to allow the S. cerevisiae cells to grow without the requirement for supplementation by sterols such as ergosterol. These variant enzymes, therefore, are highly suitable for the efficient production of isoprenoids.
摘要翻译：本发明涉及变体角鲨烯合成酶，包括酿酒酵母角鲨烯合成酶，以及编码这些变体酶的核酸分子。这些变体酶以比野生型酶更低的速率产生角鲨烯，允许更多的法呢焦磷酸被用于生产类异戊二烯化合物，同时仍然产生足够的角鲨烯以允许酿酒酵母细胞生长而不需要通过甾醇补充如麦角甾醇。因此，这些变体酶非常适合异戊二烯的高效生产。

7. 发明申请

WO2008008793A3 METHODS AND COMPOSITIONS FOR DEGRADATION OF LIGNOCELLULOSIC MATERIAL 审中-公开
标题翻译：纤维素材料降解的方法和组合物
公开(公告)号：WO2008008793A3
公开(公告)日：2008-10-09
申请号：PCT/US2007073180
申请日：2007-07-10
申请人： DYADIC INTERNAT INC , MAGNUSON JON , BURLINGAME RICHARD , SINITSYN ARKADY
发明人： MAGNUSON JON , BURLINGAME RICHARD , SINITSYN ARKADY
IPC分类号： C12P19/00 , C07H21/04 , C12N1/20 , C12N9/00 , C12N9/14 , C12N15/00
CPC分类号： C12P19/02 , C12N9/2428 , C12N9/2445 , C12P19/20 , C12Y302/01003 , C12Y302/01021 , C12Y302/01037 , Y02E50/16
摘要： Methods to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material are provided, as well as novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass.
摘要翻译：提供了将木质纤维素生物质转化成可降解木质纤维素材料的酶的方法，以及酶的新型组合，包括提供来自植物生物质的糖的协同释放的酶。

8. 发明申请

WO2008008793A2 METHODS AND COMPOSITIONS FOR DEGRADATION OF LIGNOCELLULOSIC MATERIAL 审中-公开
标题翻译：用于降解木质纤维素材料的方法和组合物
公开(公告)号：WO2008008793A2
公开(公告)日：2008-01-17
申请号：PCT/US2007/073180
申请日：2007-07-10
申请人： DYADIC INTERNATIONAL INC. , MAGNUSON, Jon , BURLINGAME, Richard , SINITSYN, Arkady
发明人： MAGNUSON, Jon , BURLINGAME, Richard , SINITSYN, Arkady
IPC分类号： C12P7/10
CPC分类号： C12P19/02 , C12N9/2428 , C12N9/2445 , C12P19/20 , C12Y302/01003 , C12Y302/01021 , C12Y302/01037 , Y02E50/16
摘要： Methods to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material are provided, as well as novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass.
摘要翻译：提供了用降解木质纤维素材料的酶将木质纤维素生物质转化为可发酵糖的方法，以及酶的新型组合，包括提供从植物生物质协同释放糖的那些酶。

9. 发明申请

WO1998015633A1 CHRYSOSPORIUM CELLULASE AND METHODS OF USE 审中-公开
标题翻译：纤维素酶和使用方法
公开(公告)号：WO1998015633A1
公开(公告)日：1998-04-16
申请号：PCT/US1997017669
申请日：1997-09-30
申请人： EMALFARB, Mark, Aaron , SOLOVJEVA, Irina Vladimirovna , BEN-BASSAT, Arie , BURLINGAME, Richard, P. , CHERNOGLAZOV, Vladimir Mikhaylovich , OKOUNEV, Oleg Nicolaevich , OLSON, Philip, T. , SINITSYN, Arkady Panteleimonovich
IPC分类号： C12N15/56
CPC分类号： D06P5/158 , C11D3/38645 , C11D11/0017 , C12N9/2437 , C12R1/645 , C12Y302/01004 , D06L4/40 , D06M16/003 , D06M2101/06 , D06P5/02
摘要： The subject invention relates to novel compositions of neutral and/or alkaline cellulase and methods for obtaining neutral and/or alkaline cellulase compositions from Chrysosporium cultures, in particular Chrysosporium lucknowense. This invention also provides mutants and methods of generating mutants of Chrysosporium capable of producing neutral and/or alkaline cellulase. This invention also relates to the genes encoding the enzymes comprising the neutral and/or alkaline cellulase composition. In addition, this invention provides methods of culturing Chrysosporium to produce neutral and/or alkaline cellulases. The neutral and/or alkaline cellulase compositions of the subject invention can be used in a variety of processes including stone washing of clothing, detergent processes, deinking, color brightening, depilling and biobleaching of paper and pulp and treatment of waste streams. The present invention also relates to the isolation and purification of cellulase enzymes, having glucanase and cellobiohydrolase activity, and useful for stonewashing applications.
摘要翻译：本发明涉及中和和/或碱性纤维素酶的新型组合物和从Chrysosporium培养物，特别是Chrysosporium lucknowense获得中性和/或碱性纤维素酶组合物的方法。本发明还提供产生能够产生中性和/或碱性纤维素酶的Chrysosporium突变体的突变体和方法。本发明还涉及编码包含中性和/或碱性纤维素酶组合物的酶的基因。此外，本发明提供培养Chrysosporium以产生中性和/或碱性纤维素酶的方法。本发明的中性和/或碱性纤维素酶组合物可以用于各种工艺，包括洗衣服，洗涤剂工艺，脱墨，增色，脱色和纸和纸浆的生物漂白以及处理废物流。本发明还涉及具有葡聚糖酶和纤维二糖水解酶活性的纤维素酶的分离和纯化，并且可用于石洗应用。

10. 发明申请

WO2018053507A3 PRODUCTION OF SESQUITERPENE PRODUCTS AND RELATED MOLECULES 审中-公开
公开(公告)号：WO2018053507A3
公开(公告)日：2018-03-22
申请号：PCT/US2017/052266
申请日：2017-09-19
申请人： EVOLVA SA , JULIEN, Bryan , BURLINGAME, Richard , WARREN, Craig
发明人： JULIEN, Bryan , BURLINGAME, Richard , WARREN, Craig
IPC分类号： C12N15/00 , C12N9/00 , C12N1/00
摘要： Recombinant cells and methods for producing and compositions containing sesquiterpene products or sesquiterpenoids are disclosed.

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