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1. 发明申请

WO2012059083A4 RECOVERY IMMUNOASSAY FOR THE SIMULTANEOUS IMMUNOCHEMICAL DETERMINATION OF AN ANALYTE (ANTIGEN) AND A TREATMENT ANTIBODY TARGETING THE ANALYTE 审中-公开
公开(公告)号：WO2012059083A4
公开(公告)日：2012-08-16
申请号：PCT/DE2011001912
申请日：2011-11-01
申请人： BIOTEZ BERLIN BUCH GMBH , STROHNER PAVEL , STAATZ ANTONIA
发明人： STROHNER PAVEL , STAATZ ANTONIA
IPC分类号： G01N33/68 , G01N33/564
CPC分类号： G01N33/6854 , G01N33/564
摘要： The invention relates to an immunoassay for the simultaneous immunochemical determination of an analyte (antigen) and a treatment antibody, treatment antibody modification, or treatment antibody analog (TAB) targeting the analyte (recovery immunoassay). The immunoassay according to the invention comprises: an optionally marked capture antibody, capture antibody modification, or capture antibody analog (CAB) bound to a surface, a marked treatment antibody, a marked treatment antibody modification, or a marked treatment antibody analog (TABmarked) or a marked antibody that has the same binding epitope or the same binding domain as the treatment antibody, or the treatment antibody modification or treatment antibody analog (TAB) used for treatment, in a mixture with an excess of the unmarked treatment antibody, treatment antibody modification, or treatment antibody analog (TAB), an antigen, which binds with the capture antibody, capture antibody modification, or capture antibody analog (CAB) and the treatment antibody, treatment antibody modification, or treatment antibody analog (TAB) at various epitopes and thus forms an immunochemical sandwich, a solvent of the unmarked treatment antibody, treatment antibody modification, or treatment antibody analog (TAB) of known concentration, and an antigen solution to be added to the samples. Areas of application of the invention are medical diagnostics, treatment control, and pharmacological research.

2. 发明申请

WO2012059083A3 RECOVERY IMMUNOASSAY FOR THE SIMULTANEOUS IMMUNOCHEMICAL DETERMINATION OF AN ANALYTE (ANTIGEN) AND A TREATMENT ANTIBODY TARGETING THE ANALYTE 审中-公开
标题翻译： RECOVERY IMMUNOASSAY用于同时免疫化学测定中的分析物（抗原）和一个相对分析物靶向治疗抗体
公开(公告)号：WO2012059083A3
公开(公告)日：2012-06-28
申请号：PCT/DE2011001912
申请日：2011-11-01
申请人： BIOTEZ BERLIN BUCH GMBH , STROHNER PAVEL , STAATZ ANTONIA
发明人： STROHNER PAVEL , STAATZ ANTONIA
IPC分类号： G01N33/68 , G01N33/564
CPC分类号： G01N33/6854 , G01N33/564
摘要： The invention relates to an immunoassay for the simultaneous immunochemical determination of an analyte (antigen) and a treatment antibody, treatment antibody modification, or treatment antibody analog (TAB) targeting the analyte (recovery immunoassay). The immunoassay according to the invention comprises: an optionally marked capture antibody, capture antibody modification, or capture antibody analog (CAB) bound to a surface, a marked treatment antibody, a marked treatment antibody modification, or a marked treatment antibody analog (TABmarked) or a marked antibody that has the same binding epitope or the same binding domain as the treatment antibody, or the treatment antibody modification or treatment antibody analog (TAB) used for treatment, in a mixture with an excess of the unmarked treatment antibody, treatment antibody modification, or treatment antibody analog (TAB), an antigen, which binds with the capture antibody, capture antibody modification, or capture antibody analog (CAB) and the treatment antibody, treatment antibody modification, or treatment antibody analog (TAB) at various epitopes and thus forms an immunochemical sandwich, a solvent of the unmarked treatment antibody, treatment antibody modification, or treatment antibody analog (TAB) of known concentration, and an antigen solution to be added to the samples. Areas of application of the invention are medical diagnostics, treatment control, and pharmacological research.
摘要翻译：本发明涉及一种用于同时测定的免疫化学分析物（抗原）的免疫测定和针对分析物的抗体疗法，或Therapieantikörpersmodifikats-analogs（TAB）（恢复免疫测定）。本发明的免疫测定法包括：结合到表面任选标记的捕获抗体，Fängerantikörpermodifikat或 - 模拟（CAB）的部分，具有显着的治疗性抗体，标记的Therapieantikörpermodifikat或 - 模拟（TABmarkiert）或相同的标记抗体，相同的结合表位，或结合结构域，如抗体治疗，或具有Therapieantikörpermodifikat用于治疗或 - 模拟（TAB），混合有一个过量的未标记的治疗性抗体或Therapieantikörpermodifikats-analogs（TAB），抗原（与捕获抗体，Fängerantikörpermodifikat或 - 模拟 CAB）和TAB）结合治疗性抗体，或Therapieantikörpermodifikat - 模拟（在由此形成的已知Konze的夹层免疫化学，未标记的抗体疗法中的溶液，或Therapieantikörpermodifikats-analogs（TAB）的不同表位并添加抗原溶液以补充样品。本发明的应用领域是医学诊断，治疗控制和药理学研究。

3. 发明申请

WO0066770A3 METHOD FOR PRODUCING A CHEMICALLY AND THERMALLY STABLE SOLID PHASE MATRIX FOR POLYMERASE CHAIN REACTION (PCR) AND DNA HYBRIDISATION ASSAY 审中-公开
标题翻译：制造方法化疗和热稳定固相基质用于聚合酶链式反应（PCR）和DNA杂交测定
公开(公告)号：WO0066770A3
公开(公告)日：2001-08-02
申请号：PCT/DE0001309
申请日：2000-04-28
申请人： BIOTEZ BERLIN BUCH GMBH , STROHNER PAVEL , SCHMIDT EBERHARD , KLEIN WOLFGANG , SCHREIBER JOACHIM , BIENERT RENATE
发明人： STROHNER PAVEL , SCHMIDT EBERHARD , KLEIN WOLFGANG , SCHREIBER JOACHIM , BIENERT RENATE
IPC分类号： C12Q1/68 , G01N33/543 , B01J20/30
CPC分类号： G01N33/54393 , C12Q1/6837
摘要： The invention relates to a method for producing a chemically and thermally stable solid phase matrix, using an inert, thermally stable support material, onto which a biotinylated cross-linking agent V 1 is applied as an adhering agent, having a molecular weight >/= 500 KD, and being preferably cross-linked immunoglobulin-gamma or bovine gammaglobulin, in highly alkaline buffers at a pH value >/= 11.0 or in a highly acidic environment at a pH value
摘要翻译：本发明涉及一种用于化疗和热稳定的固相基质的制备过程中使用的惰性热稳定载体材料到其上的生物素化连接商品V具有> / = 500 KD的分子量1，优选交联的免疫球蛋白γ或牛γ球蛋白，如强碱性缓冲剂附着剂在pH为抵接> / = 11.0或在pH

4. 发明申请

WO1997024618A1 METHOD OF MARKING BIOMOLECULES USING HORSERADISH PEROXIDASE 审中-公开
标题翻译：程序用于标记生物分子辣根过氧化物酶
公开(公告)号：WO1997024618A1
公开(公告)日：1997-07-10
申请号：PCT/DE1996002511
申请日：1996-12-24
申请人： BIOTEZ BERLIN-BUCH GMBH , SCHMIDT, Eberhard , WEISS, Christa
发明人： BIOTEZ BERLIN-BUCH GMBH
IPC分类号： G01N33/532
CPC分类号： G01N33/535 , G01N2333/908 , Y10S435/964
摘要： The invention concerns a method for the enzymatic marking of biomolecules, including peptides, hormones, antibodies and other haptens, using horseradish peroxidase (HRP). Instead of uncontrolled formation of heterogenous coupling products, the method proposed calls for the enzyme to be oxidized by sodium metaperiodate and then cross-linked intermolecularly in controlled fashion, using some of the aldehyde groups formed by the oxidation, with an alpha , omega -diaminoalkane, preferably 1,13-diamino-4,7,10-trioxatridecane. This produces an aggregate, made up of 6 to 10 HRP molecules, to which the protein to be marked can be coupled, without previously separating it from the reaction mixture, via the remaining aldehyde groups. An advantage of HRP cross-linking in this way is that a higher degree of substitution can be obtained. Since the protein is not hidden behind HRP molecules, it can exert its immunological properties without any restriction and can be determined without error using spectrophotometric techniques. The sensitivity and specificity of the HRP tracer obtained is extremely high and quite comparable to that of radioactive tracers. The HRP marking method proposed thus represents a remarkable advance in the development of high-performance enzymatic immunological detection systems.
摘要翻译：本发明涉及一种新方法，酶促生物分子，包括肽，激素，抗体或其他半抗原的标记，与辣根过氧化物酶（HRP）。代替不受控制的形成的异构偶合产物的是通过本发明的方法通过在受控的方式偏高碘酸钠氧化酶第一分子间通过利用与α，ω-二氨基烷烃形成的醛基团的一部分，优选1,13-二氨基-4,7- ，10三氧烷，网络化。从而聚合产生，其由6至10个HRP分子要被标记的蛋白质被耦合，而不脱离在剩余的醛基反应混合物之前分离。正如在前面的HRP交联的一个优点是说，具有高取代度可以实现的。因为蛋白质不受HRP分子屏蔽，其可以非限制性地发挥其免疫学性质和是无差错的分光光度测量。所得HRP示踪剂的灵敏度和特异性是可比非常高和放射性示踪剂。这代表了新的HRP标记过程代表的高性能发展的显着的进步酶免疫学检测系统。

5. 发明申请

WO2012059083A2 RECOVERY IMMUNOASSAY ZUR GLEICHZEITIGEN IMMUNCHEMISCHEN BESTIMMUNG EINES ANALYTEN (ANTIGEN) UND EINES GEGEN DEN ANALYTEN GERICHTETEN THERAPIEANTIKÖRPERS 审中-公开
标题翻译： RECOVERY IMMUNOASSAY用于同时免疫化学测定中的分析物（抗原）和一个相对分析物靶向治疗抗体
公开(公告)号：WO2012059083A2
公开(公告)日：2012-05-10
申请号：PCT/DE2011/001912
申请日：2011-11-01
申请人： BIOTEZ BERLIN-BUCH GMBH , STROHNER, Pavel , STAATZ, Antonia
发明人： STROHNER, Pavel , STAATZ, Antonia
IPC分类号： G01N33/68
CPC分类号： G01N33/6854 , G01N33/564
摘要： Die Erfindung bezieht sich auf einen Immunoassay zur gleichzeitigen immunchemischen Bestimmung eines Analyten (Antigen) und eines gegen den Analyten gerichteten Therapieantikörpers, Therapieantikörpersmodifikats oder -analogs (TAB) (Recovery Immunoassay). Der erfindungsgemäße Immunoassay umfasst: einen an einer Oberfläche gebundenen gegebenenfalls markierten Fängerantikörper, Fängerantikörpermodifikat bzw. -analog (CAB), einen markierten Therapieantikörper, ein markiertes Therapieantikörpermodifikat oder -analog (TABmarkiert) oder einen markierten Antikörper, der das gleiche Bindungsepitop, bzw. die gleiche Bindungsdomaine wie der Therapieantikörper, bzw. das zur Therapie eingesetzte Therapieantikörpermodifikat oder -analog (TAB) aufweist, im Gemisch mit einem Überschuss des unmarkierten Therapieantikörpers, Therapieantikörpermodifikats oder -analogs (TAB), ein Antigen, das mit dem Fängerantikörper, Fängerantikörpermodifikat oder -analog (CAB) und dem Therapieantikörper, Therapieantikörpermodifikat oder -analog (TAB) an verschiedenen Epitopen bindet und dadurch einen immunochemischen Sandwich bildet, eine Lösung des unmarkierten Therapieantikörpers, Therapieantikörpermodifikats oder -analogs (TAB) bekannter Konzentration und eine Antigenlösung zur Aufstockung der Proben. Anwendungsgebiete der Erfindung sind die medizinische Diagnostik, die Therapiekontrolle und die pharmakologische Forschung.
摘要翻译：本发明涉及一种用于同时测定的免疫化学分析物（抗原）的免疫测定和针对分析物的抗体疗法，或Therapieantikörpersmodifikats-analogs（TAB）（恢复免疫测定）。本发明的免疫测定法包括：结合到表面任选标记的捕获抗体，Fängerantikörpermodifikat或 - 模拟（CAB）的部分，具有显着的治疗性抗体，标记的Therapieantikörpermodifikat或 - 模拟（TABmarkiert）或相同的标记抗体，相同的结合表位，或结合结构域，如抗体治疗，或具有Therapieantikörpermodifikat用于治疗或 - 模拟（TAB），混合有一个过量的未标记的治疗性抗体或Therapieantikörpermodifikats-analogs（TAB），抗原（与捕获抗体，Fängerantikörpermodifikat或 - 模拟 CAB）和TAB）结合治疗性抗体，或Therapieantikörpermodifikat - 模拟（在由此形成的已知Konze的夹层免疫化学，未标记的抗体疗法中的溶液，或Therapieantikörpermodifikats-analogs（TAB）的不同表位 ntration和用于分配的抗原溶液到样品中。本发明的应用是医学诊断，治疗控制和药理学研究。

6. 发明申请

WO2004018707A3 VERFAHREN ZUM NACHWEIS VON EINZELNUKLEOTID-POLYMORPHISMEN (SNP) IN GENEN DES ARZNEIMITTELMETABOLISMUS UND TESTKIT ZUR DURCHFÜHRUNG DES VERFAHRENS 审中-公开
公开(公告)号：WO2004018707A3
公开(公告)日：2004-03-04
申请号：PCT/DE2003/002699
申请日：2003-08-07
申请人： BIOTEZ BERLIN-BUCH GMBH , NEUNABER, Ralf , STROHNER, Pavel , SCHREIBER, Joachim , VOIGT, Gesine , SCHUNCK, Wolf-Hagen
发明人： NEUNABER, Ralf , STROHNER, Pavel , SCHREIBER, Joachim , VOIGT, Gesine , SCHUNCK, Wolf-Hagen
IPC分类号： C12Q1/68
摘要： Die Erfindung betrifft ein vereinfachtes Verfahren zum Nachweis von SNP in Cytochrom P450 (CYP)-Genen des Menschen und ein Test Kit mit folgenden Komponenten: 1. Neu entwickelte synthetische Oligonukleotide, die geeignet sind, Genabschnitte in Genen des Arzneimittelmetabolismus mit Hilfe der „DNA Polymerase Chain Reaction" Technologie (PCR) isoformspezifisch (IS-PCR) aus genomischer Leukozyten-DNA zu amplifizieren, 2. Komponenten, die es ermöglichen, einzelsträngig biotinierte IS-PCR Produkte auf Streptavidin beschichteten Mikrotiterplatten (SA-MTP) selektiv, thermostabil und einzelsträngig zu immobilisieren, 3. testoptimierte, allelspezifische, Fluoresceinisothiocyanat (FITC) markierte synthetische Oligonukleotide, die durch Hybridisierung, nachfolgende stringente Waschung und anschließende Detektion mittels Fluorometrie bzw. Photometrie eine sichere Genotypisierung der irrmobilisierten Amplifikationsprodukte (Amplikons) gewährleisten, oder alternativ dazu, 4. Streptavidin beschichtete Glasobjektträger (SA-Chip) auf denen biotinierte, isoformspezifische, synthetische Oligonukleotide immobilisiert sind, welche mit partiell einzelsträngigen PCR Produkten hybridisiert werden, die durch diskriminierende Verlängerung eines allelspezifischen, endständig FITC markierten Oligonukleotides mittels Taq DNA Polymerase erzeugt wurden und durch nachfolgende stringente Waschung und anschließende Detektion mittels Fluorometrie bzw. Photometrie eine sichere Genotypisierung der hybridisierten Amplikons gewährleisten. Im Unterschied zu den bekannten „high-throughput"-Verfahren ermöglicht es die hier beschriebene Erfindung, auch an wenigen Einzelpatienten bei niedrigen Personal- und Sachkosten die therapeutisch wichtigen Befunde zur individuellen Ausstattung mit den verschiedenen CYP Allelen zu erheben.

7. 发明申请

WO2004018707A2 METHOD FOR IDENTIFYING SINGLE NUCLEOTIDE POLYMORPHISMS (SNP) IN GENES WHICH METABOLIZE MEDICAMENTS AND TEST KIT FOR CARRYING OUT SAID METHOD 审中-公开
标题翻译： METHOD FOR单核苷酸多态性毒品和代谢试验试剂盒用于实施所述方法的基因检测（SNP）
公开(公告)号：WO2004018707A2
公开(公告)日：2004-03-04
申请号：PCT/DE0302699
申请日：2003-08-07
申请人： BIOTEZ BERLIN BUCH GMBH , NEUNABER RALF , STROHNER PAVEL , SCHREIBER JOACHIM , VOIGT GESINE , SCHUNCK WOLF-HAGEN
发明人： NEUNABER RALF , STROHNER PAVEL , SCHREIBER JOACHIM , VOIGT GESINE , SCHUNCK WOLF-HAGEN
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6858 , C12Q2565/518 , C12Q2563/131 , C12Q2535/125 , C12Q2535/131
摘要： The invention relates to a simplified method for identifying SNP in human cytochrome P450 (CYP) genes and to a test kit comprising the following components: 1. Novel synthetic oligonucleotides, suitable for amplifying gene segments in genes that metabolize medicaments, with the aid of isoform-specific (IS-PCR) DNA polymerase chain reaction technology (PCR), from genomic leukocyte DNA, 2. Components enabling the selective, thermostable, single-stranded immobilization of biotinated IS-PCR products on microplates coated with streptavidin (SA-MTP), 3. Test optimized, allele-specific, fluorescein isothiocyanate (FITC) labeled synthetic oligonucleotides, which guarantee a reliable genotyping of the immobilized amplification products (amplicons), by hybridization, subsequent stringent washing and finally detection using fluorometry or photometry, or alternatively 4. Streptavidin coated glass object carrier (SA chip), on which biotinated, isoform-specific, synthetic oligonucleotides are immobilized, which are hybridized with partially single-stranded PCR products that have been produced by a discriminatory extension of an allele-specific, terminally FITC labeled oligonucleotide using Taq DNA Polymerase and which guarantee a reliable genotyping of the immobilized amplicons by subsequent stringent washing and finally detection using fluorometry or photometry. In comparison with the known high-throughput method, said invention enables clinical findings, which are of therapeutic importance for an individual provision of the different CYP alleles, to be established at low cost in terms of personnel and resources, even for small numbers of individual patients.
摘要翻译：本发明涉及一种用于在细胞色素P450（CYP）的人力和测试试剂盒具有以下组分的基因检测的SNP的简化方法：1.新开发的合成寡核苷酸，其能够基因序列中使用“DNA聚合酶药物代谢的基因链式反应“（PCR）技术isoformspezifisch（IS-PCR）从基因组白细胞DNA扩增，第二部件，其允许在链亲和素包被微量滴定板（SA-MTP）的单链生物素化的IS-PCR产物选择性地加热稳定的和单链，以固定3.测试优化的，等位基因特异性，异硫氰酸荧光素（FITC）标记的通过杂交保证，随后严格洗涤和随后的检测合成的寡核苷酸通过irrmobilisierten扩增产物（扩增子），或可替代地，链霉4.荧光BESC和光度测量的可靠的基因分型 hichtete载玻片（SA-Chip）的生物素化在其上，同种型，合成的寡核苷酸被固定，其杂交与Taq DNA聚合酶的手段通过等位基因特异性，末端FITC标记寡核苷酸的歧视性扩展生成部分单链PCR产物，并通过随后的严格性洗涤和确保荧光和光度测量杂交扩增可靠的检测分型。在与已知的“高通量”过程使本文所描述的本发明，并且在几个个别患者低劳动力和材料成本上升的等位基因，所述治疗重要发现单个设备与各种CYP。

8. 发明申请

WO9855864A3 SURFACES COATED WITH STREPTAVIDIN/AVIDIN 审中-公开
标题翻译：表面涂层与STREPTAVIDIN / AVIDIN
公开(公告)号：WO9855864A3
公开(公告)日：1999-03-04
申请号：PCT/DE9801527
申请日：1998-06-04
申请人： BIOTEZ BERLIN BUCH GMBH , STROHNER PAVEL , IMMER ULRIKE
发明人： STROHNER PAVEL , IMMER ULRIKE
IPC分类号： G01N33/543 , G01N33/53
CPC分类号： G01N33/54393 , Y10S435/969 , Y10S530/81 , Y10S530/811 , Y10S530/812 , Y10S530/813 , Y10S530/814 , Y10S530/815 , Y10S530/816 , Y10T428/2991 , Y10T436/25 , Y10T436/2525 , Y10T436/255
摘要： The invention relates to surfaces coated with streptavidin/avidin used in medical diagnostics and the pharmaceutical industry. The invention seeks to provide a coated solid phase which has high surface binding yields and sufficient stability, even in the presence of detergents, and which can have universal application and facilitates the necessary sensitivity for the determination procedure to be conducted. The inventive surfaces coated with stretavidin/avidin are characterized in that the coating consists of stretavidin and/or avidin, which is bonded on the surface of a solid supporting material by means of a biotinylated adhering agent.
摘要翻译：本发明涉及涂覆有用于免疫测定的链霉抗生物素蛋白和抗生物素蛋白的表面，其中所述表面包含通过生物素化粘附剂粘合在固体支持材料的表面上的链霉抗生物素蛋白和抗生物素蛋白层。

9. 发明授权

EP0877938B2 VERFAHREN ZUR MARKIERUNG VON BIOMOLEKÜLEN MIT MEERRETTICHPEROXIDASE 失效
标题翻译：程序用于标记生物分子辣根过氧化物酶
公开(公告)号：EP0877938B2
公开(公告)日：2004-04-21
申请号：EP96946118.5
申请日：1996-12-24
申请人： Biotez Berlin-Buch GmbH
发明人： SCHMIDT, Eberhard , WEISS, Christa
IPC分类号： G01N33/532 , G01N33/535
CPC分类号： G01N33/535 , G01N2333/908 , Y10S435/964
摘要： The invention concerns a method for the enzymatic marking of biomolecules, including peptides, hormones, antibodies and other haptens, using horseradish peroxidase (HRP). Instead of uncontrolled formation of heterogenous coupling products, the method proposed calls for the enzyme to be oxidized by sodium metaperiodate and then cross-linked intermolecularly in controlled fashion, using some of the aldehyde groups formed by the oxidation, with an α,φ-diaminoalkane, preferably 1,13-diamino-4,7,10-trioxatridecane. This produces an aggregate, made up of 6 to 10 HRP molecules, to which the protein to be marked can be coupled, without previously separating it from the reaction mixture, via the remaining aldehyde groups. An advantage of HRP cross-linking in this way is that a higher degree of substitution can be obtained. Since the protein is not hidden behind HRP molecules, it can exert its immunological properties without any restriction and can be determined without error using spectrophotometric techniques. The sensitivity and specificity of the HRP tracer obtained is extremely high and quite comparable to that of radioactive tracers. The HRP marking method proposed thus represents a remarkable advance in the development of high-performance enzymatic immunological detection systems.

10. 发明授权

EP0986755B1 STREPTAVIDIN/AVIDIN BESCHICHTETE OBERFLÄCHEN 失效
标题翻译： STREPTAVIDIN /抗生物素蛋白涂漆表面
公开(公告)号：EP0986755B1
公开(公告)日：2005-10-26
申请号：EP98936100.1
申请日：1998-06-04
申请人： Biotez Berlin-Buch GmbH
发明人： STROHNER, Pavel , IMMER, Ulrike
IPC分类号： G01N33/53
CPC分类号： G01N33/54393 , Y10S435/969 , Y10S530/81 , Y10S530/811 , Y10S530/812 , Y10S530/813 , Y10S530/814 , Y10S530/815 , Y10S530/816 , Y10T428/2991 , Y10T436/25 , Y10T436/2525 , Y10T436/255
摘要： The invention relates to surfaces coated with streptavidin/avidin used in medical diagnostics and the pharmaceutical industry. The invention seeks to provide a coated solid phase which has high surface binding yields and sufficient stability, even in the presence of detergents, and which can have universal application and facilitates the necessary sensitivity for the determination procedure to be conducted. The inventive surfaces coated with stretavidin/avidin are characterized in that the coating consists of stretavidin and/or avidin, which is bonded on the surface of a solid supporting material by means of a biotinylated adhering agent.

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