专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

3. 发明申请

WO2014032695A1 ENZYME KINETICS 审中-公开
标题翻译： ENZYME动力学
公开(公告)号：WO2014032695A1
公开(公告)日：2014-03-06
申请号：PCT/EP2012/066650
申请日：2012-08-28
申请人： FOSS ANALYTICAL A/S , DANMARKS TEKNISKE UNIVERSITET , BAUM, Andreas , MIKKELSEN, Jørn , EGEBO, Max Sass
发明人： BAUM, Andreas , MIKKELSEN, Jørn , EGEBO, Max Sass
IPC分类号： C12Q1/00 , G06F19/12
CPC分类号： C12Q1/00 , G06F19/702 , G06F19/707
摘要： A method of determining an indication of enzymatic kinetics of a monitored enzymatic reaction comprises: originating temporal evolution data, particularly FTIR spectral fingerprint evolution data, of one or both of a substrate and a product of an enzymatic reaction for different known enzymatic activities; establishing in a data processor a calibration correlating enzymatic kinetics with temporal evolution data by applying a chemometric multi-way model to data representative of the originated temporal evolution data; originating corresponding temporal evolution data for a monitored enzymatic reaction of unknown kinetics; providing to the data processor data representing the provided temporal evolution data; and applying to that data the calibration to determine thereby in the data processor a quantitative and/or qualitative indication of enzymatic kinetics of the monitored enzymatic reaction.
摘要翻译：确定所监测的酶促反应的酶动力学指示的方法包括：对不同已知酶活性的底物和酶反应产物之一或两者起始时间进化数据，特别是FTIR光谱指纹进化数据; 在数据处理器中建立校准，使酶动力学与时间演化数据相关联，通过将化学计量多路模型应用于代表起始时间进化数据的数据; 产生用于未知动力学的被监测的酶反应的相应的时间演变数据; 向所述数据处理器提供表示所提供的时间演进数据的数据; 并且对该数据应用该校准，从而在数据处理器中确定所监测的酶反应的酶动力学的定量和/或定性指示。

IPRDB

热门服务

关于我们

友情链接

联系方式