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1. 发明申请

US20130324436A1 PROCEDURE FOR NUCLEIC ACID-BASED DIAGNOSTIC DETERMINATION OF BACTERIAL GERM COUNTS AND KIT FOR THIS PURPOSE 审中-公开
标题翻译：基于核酸的诊断方法确定细菌种类和用于本目的的试剂盒
公开(公告)号：US20130324436A1
公开(公告)日：2013-12-05
申请号：US13990242
申请日：2010-11-30
申请人： Gabor Kiss , Janos Kiss , Timea Kiss , Ambrusne Sztancsik Katalin Kovacs , Georgina Bernath
发明人： Gabor Kiss , Janos Kiss , Timea Kiss , Ambrusne Sztancsik Katalin Kovacs , Georgina Bernath
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q1/686 , C12Q2600/16 , C12Q2561/113 , C12Q2563/107 , C12Q2565/102
摘要： Disclosed are procedures and kits for nucleic acid-based molecular diagnostic determination of bacterial germ counts during which procedure evolutionarily conserved genes and genes coding for characteristic pathogenicity markers, favourably microbial enzyme, toxin, special resistance, are detected using real-time PCR amplification method with the application of fluorescent hydrolysis probes. The multiplication of nucleotide chains takes place with oligonucleotides annealing to the structural gene 5′ end region and to the adjacent upstream regulatory promoter-operator region so that the presence of the structural gene is shown along with the adjacent upstream regulatory promoter-operator sequences; the functional nature of the structural gene is simultaneously checked. The result is measured with a genome unit equivalent DNA amount calibrated to the germ number of sample units equivalent to standard procedures. The calibrated determination of bacterial germ counts is favourably based on single copy gene sequences in the genome, like those coding for characteristic pathogenicity markers.
摘要翻译：公开了用于基于核酸的分子诊断测定细菌胚细胞计数的程序和试剂盒，在此过程中，使用实时PCR扩增方法检测进化保守基因和编码特征性致病性标志物的基因，有利于微生物酶，毒素，特异性抗性，应用荧光水解探针。核苷酸链的增殖是通过对结构基因5'末端区域和相邻的上游调控启动子 - 操纵子区域退火的寡核苷酸进行的，从而显示结构基因的存在以及相邻的上游调控启动子 - 操纵子序列; 同时检查结构基因的功能性质。结果用基准单位等效DNA量来测量，该DNA量与标准程序相当的样品单位的胚芽数量校准。根据基因组中的单拷贝基因序列，如编码特征性致病性标记物的那些，校准的细菌菌数的测定是有利的。

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