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1. 发明申请

US20150305341A1 COMPOSITIONS AND METHODS TO CONTROL INSECT PESTS 审中-公开
标题翻译：组合物及其控制方法
公开(公告)号：US20150305341A1
公开(公告)日：2015-10-29
申请号：US14793893
申请日：2015-07-08
申请人： Karen E. Broglie , Kevin Kriss , Albert L. Lu , Mani Muthalagi , James Kevin Presnail
发明人： Karen E. Broglie , Kevin Kriss , Albert L. Lu , Mani Muthalagi , James Kevin Presnail
IPC分类号： A01N57/16 , A01N65/20 , A01N65/44 , C12N15/82 , C12N15/113
CPC分类号： A01N57/16 , A01N63/00 , A01N65/20 , A01N65/44 , C12N15/113 , C12N15/8218 , C12N15/8286 , C12N2310/11 , C12N2310/531 , Y02A40/162
摘要： Methods and compositions are provided which employ a silencing element that, when ingested by a pest, such as a Coleopteran plant pest or a Diabrotica plant pest, decrease the expression of a target sequence in the pest. In specific embodiments, the decrease in expression of the target sequence controls the pest and thereby the methods and compositions are capable of limiting damage to a plant. The present invention provides various target polynucleotides set forth in any one of SEQ ID NOS: 1-236 or active variants and fragments thereof, wherein a decrease in expression of one or more the sequences in the target pest controls the pest (i.e., has insecticidal activity). Further provided are silencing elements which when ingested by the pest decrease the level of the target polypeptide and thereby control the pest. In specific embodiment, the pest is D. virgifera virgifera, D. barberi, D. speciosa, or D. undecimpunctata howardi. Plants, plant part, bacteria and other host cells comprising the silencing elements or an active variant or fragment thereof of the invention are also provided.
摘要翻译：提供了使用沉积元件的方法和组合物，当由有害生物摄取的诸如鞘翅目植物病虫害（Diaprotica plant pest）的害虫时，会降低害虫中靶序列的表达。在具体实施方案中，靶序列的表达降低控制有害生物，因此方法和组合物能够限制对植物的损害。本发明提供了SEQ ID NO：1-236中任一项所述的各种靶多核苷酸或其活性变体及其片段，其中目标害虫中一个或多个序列的表达降低控制害虫（即具有杀虫活性活动）。进一步提供的是沉积元件，当受害虫摄入时，降低靶多肽的水平，从而控制害虫。在具体实施方案中，害虫是D. virgifera virgifera，D.barberi，D. speciosa或D. undecimpunctata howardi。还提供了包含本发明的沉默元件或其活性变体或片段的植物，植物部分，细菌和其它宿主细胞。

2. 发明授权

US08895716B2 Viral promoter, truncations thereof, and methods of use 有权
标题翻译：病毒启动子，其截短物和使用方法
公开(公告)号：US08895716B2
公开(公告)日：2014-11-25
申请号：US13332439
申请日：2011-12-21
申请人： Scott Diehn , Albert L. Lu , Carl R. Simmons
发明人： Scott Diehn , Albert L. Lu , Carl R. Simmons
IPC分类号： C07H21/04 , C12N15/82 , C12N15/87 , C12N15/00 , C12N5/10 , A01H5/00
CPC分类号： C12N15/8225 , C12N2730/00043
摘要： The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本公开提供了用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将与本发明的启动子可操作地连接的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

3. 发明授权

US08710296B2 Defensin polynucleotides and methods of use 有权
标题翻译：防御素多核苷酸和使用方法
公开(公告)号：US08710296B2
公开(公告)日：2014-04-29
申请号：US13074416
申请日：2011-03-29
申请人： Carl R. Simmons , Pedro A. Navarro Acevedo , Leslie Harvell , Rebecca Cahoon , Billy Fred McCutchen , Albert L. Lu , Rafael Herrmann , James F. H. Wong
发明人： Carl R. Simmons , Pedro A. Navarro Acevedo , Leslie Harvell , Rebecca Cahoon , Billy Fred McCutchen , Albert L. Lu , Rafael Herrmann , James F. H. Wong
IPC分类号： A01H5/00 , A01H1/00 , C07H21/04 , C12N15/82 , C12N1/00
CPC分类号： C07K14/4723 , C07K14/415 , C12N15/8279
摘要： Methods and compositions for modulating development and defense responses are provided. Nucleotide sequences encoding defensin proteins are provided. The sequences can be used in expression cassettes for modulating development, developmental pathways, and defense responses. Transformed plants, plant cells, tissues, and seed are also provided.
摘要翻译：提供了调节发展和防御反应的方法和组成。提供编码防御素蛋白质的核苷酸序列。序列可用于表达盒用于调节发育，发育途径和防御反应。还提供了转化的植物，植物细胞，组织和种子。

4. 发明授权

US08344206B2 Viral promoter, truncations thereof, and methods of use 有权
公开(公告)号：US08344206B2
公开(公告)日：2013-01-01
申请号：US12775669
申请日：2010-05-07
申请人： Scott Diehn , Albert L. Lu , Carl R. Simmons
发明人： Scott Diehn , Albert L. Lu , Carl R. Simmons
IPC分类号： C12N15/82 , C12N15/113 , C12N5/10
CPC分类号： C12N15/8216
摘要： The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

5. 发明申请

US20120167250A1 VIRAL PROMOTER, TRUNCATIONS THEREOF, AND METHODS OF USE 有权
公开(公告)号：US20120167250A1
公开(公告)日：2012-06-28
申请号：US13332439
申请日：2011-12-21
申请人： Scott Diehn , Albert L. Lu , Carl R. Simmons
发明人： Scott Diehn , Albert L. Lu , Carl R. Simmons
IPC分类号： A01H1/06 , A01H5/10 , C12N5/10 , A01H5/00 , C12N15/113 , C12N15/82
CPC分类号： C12N15/8225 , C12N2730/00043
摘要： The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.

6. 发明申请

US20120167249A1 VIRAL PROMOTER, TRUNCATIONS THEREOF, AND METHODS OF USE 有权
标题翻译：司法促进者，其职权及其使用方法
公开(公告)号：US20120167249A1
公开(公告)日：2012-06-28
申请号：US13329511
申请日：2011-12-19
申请人： Scott Diehn , Albert L. Lu , Carl R. Simmons
发明人： Scott Diehn , Albert L. Lu , Carl R. Simmons
IPC分类号： A01H1/00 , C12N15/82 , A01H5/00 , A01H5/10 , C12N15/63 , C12N5/04
CPC分类号： C12N15/8226 , C12N15/8216 , C12N15/8227 , C12N15/8286 , Y02A40/162
摘要： The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequence disclosed herein is provided. The method comprises stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本公开提供了用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括将与本发明的启动子可操作地连接的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

7. 发明授权

US08026415B2 Defensin polynucleotides and methods of use 有权
公开(公告)号：US08026415B2
公开(公告)日：2011-09-27
申请号：US12132536
申请日：2008-06-03
申请人： Carl R. Simmons , Pedro A. Navarro Acevedo , Leslie Harvell , Rebecca Cahoon , Billy Fred McCutchen , Albert L. Lu , Rafael Herrmann , James F. H. Wong
发明人： Carl R. Simmons , Pedro A. Navarro Acevedo , Leslie Harvell , Rebecca Cahoon , Billy Fred McCutchen , Albert L. Lu , Rafael Herrmann , James F. H. Wong
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82 , C12N15/29
CPC分类号： C07K14/4723 , C07K14/415 , C12N15/8279
摘要： Methods and compositions for modulating development and defense responses are provided. Nucleotide sequences encoding defensin proteins are provided. The sequences can be used in expression cassettes for modulating development, developmental pathways, and defense responses. Transformed plants, plant cells, tissues, and seed are also provided.

8. 发明申请

US20110179522A1 DEFENSIN POLYNUCLEOTIDES AND METHODS OF USE 有权
标题翻译：防御素多核苷酸和使用方法
公开(公告)号：US20110179522A1
公开(公告)日：2011-07-21
申请号：US13074416
申请日：2011-03-29
申请人： Carl R. Simmons , Pedro A. Navarro Acevedo , Leslie Harvell , Rebecca Cahoon , Billy Fred McCutchen , Albert L. Lu , Rafael Herrmann , James F. H. Wong
发明人： Carl R. Simmons , Pedro A. Navarro Acevedo , Leslie Harvell , Rebecca Cahoon , Billy Fred McCutchen , Albert L. Lu , Rafael Herrmann , James F. H. Wong
IPC分类号： C12N15/82 , C07H21/04 , C12N15/63 , C12N5/10 , A01H5/00 , A01H5/10
CPC分类号： C07K14/4723 , C07K14/415 , C12N15/8279
摘要： Methods and compositions for modulating development and defense responses are provided. Nucleotide sequences encoding defensin proteins are provided. The sequences can be used in expression cassettes for modulating development, developmental pathways, and defense responses. Transformed plants, plant cells, tissues, and seed are also provided.
摘要翻译：提供了调节发展和防御反应的方法和组成。提供编码防御素蛋白质的核苷酸序列。序列可用于表达盒用于调节发育，发育途径和防御反应。还提供了转化的植物，植物细胞，组织和种子。

9. 发明申请

US20110154526A1 Maize event DP-043A47-3 and methods for detection thereof 审中-公开
标题翻译：玉米事件DP-043A47-3及其检测方法
公开(公告)号：US20110154526A1
公开(公告)日：2011-06-23
申请号：US12970614
申请日：2010-12-16
申请人： Scott Diehn , Albert L. Lu , Timothy M. Nowatzki , Douglas S. Nubel , M. Alejandra Pascual , James C. Register, III , Christopher J. Scelonge , Gregory J. Young , Joshua K. Young , Cathy Xiaoyan Zhong
发明人： Scott Diehn , Albert L. Lu , Timothy M. Nowatzki , Douglas S. Nubel , M. Alejandra Pascual , James C. Register, III , Christopher J. Scelonge , Gregory J. Young , Joshua K. Young , Cathy Xiaoyan Zhong
IPC分类号： A01H1/00 , C12N15/63 , A01H5/00 , C07H21/04 , C12N5/04 , C12Q1/68 , G01N33/50 , C08B30/00 , A23L1/09 , A23D9/00 , A23L1/10 , A01G1/00 , A01C7/00 , A01D46/00
CPC分类号： C12N15/8286 , C12Q1/6895 , Y02A40/162 , Y10T436/143333
摘要： The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-043A47-3 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
摘要翻译：本发明提供了涉及转基因抗虫玉米植物的DNA组合物。还提供了基于插入到玉米基因组中的重组构建体的DNA序列和插入位点侧翼的DNA序列来检测玉米DP-043A47-3事件的存在的测定法。提供了用于进行测定的试剂盒和条件。

10. 发明申请

US20110154525A1 Maize event DP-040416-8 and methods for detection thereof 审中-公开
标题翻译：玉米事件DP-040416-8及其检测方法
公开(公告)号：US20110154525A1
公开(公告)日：2011-06-23
申请号：US12970537
申请日：2010-12-16
申请人： Scott Diehn , Albert L. Lu , Timothy M. Nowatzki , Douglas S. Nubel , M. Alejandra Pascual , James C. Register, III , Christopher J. Scelonge , Gregory J. Young , Joshua K. Young , Cathy Xiaoyan Zhong
发明人： Scott Diehn , Albert L. Lu , Timothy M. Nowatzki , Douglas S. Nubel , M. Alejandra Pascual , James C. Register, III , Christopher J. Scelonge , Gregory J. Young , Joshua K. Young , Cathy Xiaoyan Zhong
IPC分类号： A01G7/00 , A01H5/00 , A01H5/10 , C07H21/04 , A01H1/04 , C12N15/63 , C12N5/04 , C12Q1/68 , G01N33/50 , A01C7/18 , A01D46/00
CPC分类号： C12N15/8286 , A01H1/02 , Y02A40/162 , Y10T436/143333
摘要： The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-040416-8 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
摘要翻译：本发明提供了涉及转基因抗虫玉米植物的DNA组合物。还提供了基于插入到玉米基因组中的重组构建体的DNA序列和插入位点侧翼的DNA序列来检测玉米DP-040416-8事件的存在的测定法。提供了用于进行测定的试剂盒和条件。

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