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1. 发明申请

US20060118488A1 Apparatus and method of utilizing a sawed tooth shaped gradient for chromatographic separation 审中-公开
标题翻译：利用锯齿形梯度进行色谱分离的装置和方法
公开(公告)号：US20060118488A1
公开(公告)日：2006-06-08
申请号：US11153862
申请日：2005-06-15
申请人： Aaron Timperman , Daniel Morris
发明人： Aaron Timperman , Daniel Morris
IPC分类号： B01D15/08
CPC分类号： G01N30/34 , B01D15/166 , B01J20/286 , B01J20/287 , B01J20/288 , G01N30/02 , B01D15/34
摘要： The present invention provides an apparatus and a method wherein the movement of analytes on a chromatographic separation column is controlled by the manipulation of isocratic and gradient flow. Such manipulation allows for a distinct set of analytes to be eluted from the chromatography column and delivered downstream for further separation and/or processing while the remainder of the sample remains in a “holding pattern” on the chromatography column. As such, the present invention allows for a small portion of the sample to be processed downstream of the column while substantially eliminating undesirable isocratic elution from the column during such downstream processing. Once the downstream processing has been completed, the column of the present invention elutes a second distinct analyte and the remainder of the sample is maintained in a holding pattern. The process may be repeated until the entire sample has been eluted from the chromatography column.
摘要翻译：本发明提供了一种装置和方法，其中分析物在色谱分离柱上的移动通过等度梯度流的操纵来控制。这样的操作允许从色谱柱洗脱出一组不同的分析物，并在下游进行进一步分离和/或处理，同时样品的其余部分保留在色谱柱上的“保持模式”中。因此，本发明允许样品的一小部分在柱下游进行处理，同时基本上消除了在这种下游处理期间从柱中不期望的等度洗脱。一旦下游处理已经完成，本发明的色谱柱洗脱第二种不同的分析物，并且样品的其余部分保持在保持图案中。可以重复该过程，直到整个样品已经从色谱柱洗脱出来。

2. 发明申请

US20080076143A1 Microfluidic system for proteome analysis 审中-公开
标题翻译：用于蛋白质组分析的微流体系统
公开(公告)号：US20080076143A1
公开(公告)日：2008-03-27
申请号：US11729437
申请日：2007-03-28
申请人： Aaron Timperman
发明人： Aaron Timperman
IPC分类号： C07K16/00 , C12Q1/37
CPC分类号： G01N27/44791 , B01L3/5027 , B01L3/502707 , B01L3/502753 , B01L2200/10 , B01L2400/0415 , G01N27/44717 , G01N27/44743 , G01N27/44773 , G01N33/6803
摘要： The invention provides a microfluidic system and method to rapidly analyze large numbers of compounds or complex mixtures of compounds, particularly, low abundance cellular proteins involved in cell signaling pathways. In one aspect, an integrated microfluidic system comprises an upstream separation module (preferably, a multi-dimensional separation device), a microfluidic device for on-device protein digestion of substantially separated proteins received from the upstream separation module, a downstream separation module for separating digestion products of said proteins, a peptide analysis module and a processor for determining the amino acid sequence of said proteins. Preferably, the system comprises an interfacing microfluidic device between the downstream separation module and the peptide analysis module.
摘要翻译：本发明提供了一种微流体系统和方法，用于快速分析大量化合物或化合物的复合混合物，特别是涉及细胞信号通路的低丰度细胞蛋白质。在一个方面，集成的微流体系统包括上游分离模块（优选地，多维分离装置），用于从上游分离模块接收的基本上分离的蛋白质的装置内蛋白质消化的微流体装置，用于分离的下游分离模块所述蛋白质的消化产物，肽分析模块和用于测定所述蛋白质的氨基酸序列的处理器。优选地，该系统包括在下游分离模块和肽分析模块之间的界面微流体装置。

3. 发明申请

US20050133371A1 Apparatus and method for Edman degradation on a microfluidic device utilizing an electroosmotic flow pump 审中-公开
标题翻译：在使用电渗流泵的微流体装置上Edman降解的装置和方法
公开(公告)号：US20050133371A1
公开(公告)日：2005-06-23
申请号：US10955657
申请日：2004-09-30
申请人： Aaron Timperman
发明人： Aaron Timperman
IPC分类号： G01N20060101 , G01N27/447 , G01N27/453
CPC分类号： G01N27/44752
摘要： The present invention comprises an electroosmotic flow pump with both anion and cation exchange beads packed in separate channels that pump towards an intersection. Combining the two flow streams results in higher flowrates for the pump and allows operation of the pump over a wide pH range. The pump can be used to deliver solutions ranging from a pH of about 2 to about 12. In a preferred embodiment, the electroosmotic pump of the present invention is fabricated on a microfluidic device capable of Edman degradation. In a preferred embodiment of the present invention, the beads are immobilized in the channels using weirs and membranes, eliminating the need for frits. The pump may be comprised of capillaries. Additionally, the electroosmotic flow pump of the present invention may be integrated into an Integrated Microfluidic Proteome Analysis System.
摘要翻译：本发明包括一个电渗流量泵，阴离子和阳离子交换珠粒包装在分开的通道中，泵送到交叉口。结合两个流动流导致泵的较高流量，并允许泵在宽的pH范围内操作。泵可用于输送约2至约12的pH范围内的溶液。在优选的实施方案中，本发明的电渗泵在能够进行Edman降解的微流体装置上制造。在本发明的一个优选实施方案中，使用堰和膜将珠子固定在通道中，消除了对玻璃料的需要。泵可以由毛细管组成。另外，本发明的电渗流量泵可以集成到集成微流体蛋白质组分析系统中。

4. 发明申请

US20070158192A1 Apparatus and method for coupling microfluidic systems with a mass spectrometer utilizing rapid voltage switching 审中-公开
标题翻译：用微波流体系统与利用快速电压切换的质谱联用的装置和方法
公开(公告)号：US20070158192A1
公开(公告)日：2007-07-12
申请号：US11495905
申请日：2006-07-28
申请人： Aaron Timperman , James Lenke , Trust Razunguzwa
发明人： Aaron Timperman , James Lenke , Trust Razunguzwa
IPC分类号： H01J49/00 , C07K1/26
CPC分类号： H01J49/165 , B01L3/5027 , C07K1/26 , H01J27/04 , H01J49/0018
摘要： The invention relates to an apparatus for coupling microfluidic systems with electrospray ionization mass spectrometry utilizing a hydrodynamic flow. The invention also relates to a method of preventing a sample in a main channel of a microfluidic device from migrating down either a first side channel or a second side channel by rapidly alternating a voltage being applied to the first side channel and the second side channel.
摘要翻译：本发明涉及一种利用流体动力学流动的电喷雾质谱联用微流体系统的装置。本发明还涉及通过快速交替施加到第一侧通道和第二侧通道的电压来防止微流体装置的主通道中的样品沿第一侧通道或第二侧通道向下移动的方法。

5. 发明申请

US20060285999A1 Apparatus and method for coupling microfluidic systems with electrospray ionization mass spectrometry utilizing a hydrodynamic flow restrictor 审中-公开
标题翻译：使用流体动力学流量限制器的电喷雾质谱联用微流体系统的装置和方法
公开(公告)号：US20060285999A1
公开(公告)日：2006-12-21
申请号：US11157545
申请日：2005-06-21
申请人： Aaron Timperman
发明人： Aaron Timperman
IPC分类号： B01L3/00
CPC分类号： B01L3/502746 , B01L3/0268 , B01L2300/0816 , B01L2400/0418 , B01L2400/0487 , B01L2400/086 , H01J49/0018 , H01J49/165
摘要： A microfluidic device is disclosed wherein a hydrodynamic flow restrictor is positioned in a main channel; a make-up flow channel engages the main channel at a position between the hydrodynamic flow restrictor and an output channel. The hydrodynamic flow restrictor substantially negates a hydrodynamic backpressure in the main channel to the extent that low electroosmotic flow may be utilized in the main channel. Further, a method is disclosed wherein a sample is delivered to the main channel and low EOF drives the sample through the main channel. The method comprises positioning a hydrodynamic flow restrictor in the main channel and delivering a make-up solution via hydrodynamic flow to the main channel at a position between a hydrodynamic flow restrictor and the output channel. The hydrodynamic flow restrictor substantially negates a hydrodynamic backpressure in the main channel to the extent that low electroosmotic flow may be utilized in the main channel.
摘要翻译：公开了一种微流体装置，其中流体动力学流量限制器位于主通道中; 补充流动通道在流体动力学流量限制器和输出通道之间的位置处接合主通道。流体动力学流量限制器基本上消除了主通道中的流体动力背压，达到在主通道中可以使用低电渗流量的程度。此外，公开了一种方法，其中将样品输送到主通道并且低EOF通过主通道驱动样品。该方法包括将流体动力流量限制器定位在主通道中，并且通过流体动力流将补充溶液在流体动力学流量限制器和输出通道之间的位置处输送到主通道。流体动力学流量限制器基本上消除了主通道中的流体动力背压，达到在主通道中可以使用低电渗流量的程度。

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