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    • 7. 发明授权
    • Enhanced catalyzed reporter deposition
    • 增强催化报道沉积
    • US06372937B1
    • 2002-04-16
    • US09434742
    • 1999-11-05
    • Mark Norman BobrowKarl Edwin AdlerKevin Aaron Roth
    • Mark Norman BobrowKarl Edwin AdlerKevin Aaron Roth
    • C07C22900
    • C12Q1/28C07C235/20
    • A method for enhancing the conversion of a phenol substrate to a product by a peroxidase enzyme comprises the steps of reacting a conjugate comprising a detectably labeled phenol with a peroxidase enzyme in the presence of an enhancing reagent, the enhancing reagent conmprising an inorganic salt, an organic compound having the formula wherein when X is B(OH)2, Y is I, or wherein when X is OH, Y is a halogen, or Q-R wherein Q is a linear or branched 1-12 heteroatom alkyl wherein the heteroatoms are selected from C, N, O, and S, wherein the bonds connecting the heteroatom alkyl chain are single or double, wherein any carbon atom in the heteroatom alkyl chain optionally includes a substituent selected from —OH, —COOH, —NH2, and —SH, and wherein R is selected from —OH, —COOH, —NH2, and —CH3; or mixtures of the inorganic salt and the organic compound. A compound having the formula: wherein R1 is —CH2OH, —COOH, and n is 1-8.
    • 通过过氧化物酶增强酚底物向产物的转化的方法包括以下步骤:在增强试剂存在下使包含可检测标记的苯酚的缀合物与过氧化物酶反应,使无机盐, 当X为B(OH)2,Y为I时,或其中当X为OH时,Y为卤素或QR为有机化合物,其中Q为直链或支链1-12杂原子烷基,其中杂原子选自 C,N,O和S,其中连接杂原子烷基链的键是单或双键,其中杂原子烷基链中的任何碳原子任选地包括选自-OH,-COOH,-NH 2和-SH的取代基, 并且其中R选自-OH,-COOH,-NH 2和-CH 3; 或无机盐和有机化合物的混合物。 具有下式的化合物:其中R 1是-CH 2 OH,-COOH,n是1-8。
    • 10. 发明授权
    • Enhanced catalyzed reporter deposition
    • 增强催化报道沉积
    • US06593100B2
    • 2003-07-15
    • US09859988
    • 2001-05-17
    • Mark Norman BobrowKarl Edwin AdlerKevin Aaron Roth
    • Mark Norman BobrowKarl Edwin AdlerKevin Aaron Roth
    • C12Q128
    • C12Q1/28C07C235/20
    • A method for enhancing the conversion of a phenol substrate to a product by a peroxidase enzyme comprises the steps of reacting a conjugate comprising a detectably labeled phenol with a peroxidase enzyme in the presence of an enhancing reagent, the enhancing reagent comprising an inorganic salt, an organic compound having the formula wherein when X is B(OH)2, Y is I, or wherein when X is OH, Y is a halogen, or Q-R wherein Q is a linear or branched 1-12 heteroatom alkyl wherein tie heteroatoms are selected from C, N, O, and S, wherein the bonds connecting the heteroatom alkyl chain are single or double, wherein any carbon atom in the heteroatom alkyl chain optionally includes a substituent selected from —OH, —COOH, —NH2, and —SH, and wherein R is selected from —OH, —COOH, —NH2, and —CH3; or mixtures of the inorganic salt and the organic compound. A compound having the formula: wherein R1 is —CH2OH, —COOH, and n is 1-8.
    • 通过过氧化物酶增强酚底物转化为产物的方法包括以下步骤:在增强试剂存在下使包含可检测标记的苯酚的缀合物与过氧化物酶反应,所述增强剂包含无机盐, 当X是B(OH)2,Y是I时,或其中当X是OH,Y是卤素或Q 2时,其中Q是直链或支链1-12杂原子烷基,其中连接杂原子选自 C,N,O和S,其中连接杂原子烷基链的键是单或双键,其中杂原子烷基链中的任何碳原子任选地包括选自-OH,-COOH,-NH 2和-SH的取代基, 并且其中R选自-OH,-COOH,-NH 2和-CH 3; 或无机盐和有机化合物的混合物。具有下式的化合物:其中R 1是-CH 2 OH,-COOH,n是1-8。