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1. 发明专利

JP2010178741A METHOD AND COMPOSITION RELATED TO miRNA AND miRNA INHIBITORY MOLECULE 有权
标题翻译：与miRNA和miRNA抑制分子相关的方法和组合
公开(公告)号：JP2010178741A
公开(公告)日：2010-08-19
申请号：JP2010036925
申请日：2010-02-23
申请人： Ambion Inc , アンビオンインコーポレーティッド
发明人： BROWN DAVID , FORD LANCE , CHENG ANGIE , JARVIS RICH , BYROM MIKE , OVCHARENKO DMITRIY , DEVROE ERIC , KELNAR KEVIN
IPC分类号： C12N15/113 , A61K31/7105 , A61K31/712 , A61K48/00 , A61P9/10 , A61P11/00 , A61P25/28 , A61P35/00 , A61P37/00 , A61P43/00 , C12N15/11 , C12Q1/02 , C12Q1/68
CPC分类号： C12N15/113 , A61K9/127 , A61K31/7088 , A61K31/7105 , A61K31/713 , A61K45/06 , A61N5/10 , C12N15/111 , C12N15/1136 , C12N2310/14 , C12N2310/141 , C12N2310/312 , C12N2310/321 , C12N2310/322 , C12N2310/33 , C12N2310/344 , C12N2310/35 , C12N2310/351 , C12N2310/3527 , C12N2310/3535 , C12N2310/533 , C12N2320/12 , C12N2320/30 , C12N2320/50 , C12N2330/10 , C12Q1/68 , C12Q1/6876 , C12Q2600/158 , C12Q2600/178 , C12N2310/3521
摘要： PROBLEM TO BE SOLVED: To provide methods and compositions for introducing miRNA activity or function into cells by using synthetic nucleic acid molecules. SOLUTION: There are provided the methods and compositions for identifying the miRNAs having specific cellular functions related to therapeutic, diagnostic and prognostic applications wherein, synthetic miRNAs and/or miRNA inhibitors are used in library screening assays. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：待解决的问题：提供通过使用合成核酸分子将miRNA活性或功能引入细胞的方法和组合物。提供了用于鉴定具有与治疗，诊断和预后应用相关的特定细胞功能的miRNA的方法和组合物，其中在文库筛选测定中使用合成的miRNA和/或miRNA抑制剂。版权所有（C）2010，JPO＆INPIT

2. 发明申请

US20040033602A1 Methods and compositions relating to polypeptides with RNase III domains that mediate RNA interference 审中-公开
标题翻译：涉及介导RNA干扰的具有RNase III结构域的多肽的方法和组合物
公开(公告)号：US20040033602A1
公开(公告)日：2004-02-19
申请号：US10460775
申请日：2003-06-12
申请人： Ambion, Inc.
发明人： Lance P. Ford , David Brown
IPC分类号： A61K048/00 , C12N015/85
CPC分类号： C12N15/1135 , C12N9/22 , C12N15/111 , C12N15/113 , C12N15/1136 , C12N15/1137 , C12N2310/14 , C12N2330/30 , C12Y301/26003
摘要： The present invention concerns methods and compositions involving RNase III and polypeptides containing RNase III domains to generate RNA capable of triggering RNA-mediated interference (RNAi) in a cell. In some embodiments, the RNase III is from a prokaryote. RNase III activity will cleave a double-stranded RNA molecule into short RNA molecules that may trigger or mediate RNAi (siRNA). Compositions of the invention include kits that include an RNase III domain-containing polypeptide. The present invention further concerns methods using polypeptides with RNase III activity for generating RNA molecules that effect RNAi, including the generation of a number of RNA molecules to the same target.
摘要翻译：本发明涉及包含RNase III和含有RNase III结构域的多肽的方法和组合物，以产生能够触发细胞中RNA介导的干扰（RNAi）的RNA。在一些实施方案中，RNA酶III来自原核生物。 RNA酶III活性将双链RNA分子切割成可以引发或介导RNAi（siRNA）的短RNA分子。本发明的组合物包括包含含有RNase III结构域的多肽的试剂盒。本发明还涉及使用具有RNase III活性的多肽来产生影响RNAi的RNA分子的方法，包括向同一靶标产生大量RNA分子。

3. 发明申请

US20040219529A1 Compositions and methods of using a synthetic DNase I 有权
标题翻译：使用合成DNA酶I的组合物和方法
公开(公告)号：US20040219529A1
公开(公告)日：2004-11-04
申请号：US10420345
申请日：2003-04-22
申请人： Ambion, Inc.
发明人： Gary Latham , Jon Kemppainen
IPC分类号： C12Q001/68 , C07H021/04 , C12N009/22 , C12P019/34
CPC分类号： C12N9/22
摘要： Compositions and method for making and using a synthetic bovine DNase I are disclosed. More particularly, the sbDNase I of the present invention is a versatile enzyme that cleaves DNA nonspecifically to release 5null-phosphorylated nucleotides. The sbDNase I molecules of the present invention find particular use in a wide range of molecular biology applications, including: degradation of contaminating DNA after RNA isolation; RNA clean-up prior to, or in conjunction with, RT-PCR after in vitro transcription; identification of protein binding sequences on DNA (DNase I footprinting); prevention of clumping when handling cultured cells; tissue dissociation and creation of fragmented DNA for in vitro recombination reactions.
摘要翻译：公开了制备和使用合成牛DNase I的组合物和方法。更具体地，本发明的sbDNase I是非特异性切割DNA以释放5'-磷酸化核苷酸的通用酶。本发明的sbDNase I分子在广泛的分子生物学应用中特别有用，包括：RNA分离后污染DNA的降解; 在体外转录之前或之后与RT-PCR结合进行RNA清除; 鉴定DNA上的蛋白质结合序列（DNase I footprinting）; 处理培养细胞时防止结块; 组织解离和产生用于体外重组反应的片段DNA。

4. 发明申请

US20020055162A1 Compositions and methods for increasing the yields of in vitro RNA transcription and other polynucleotide synthetic reactions 失效
标题翻译：用于增加体外RNA转录和其他多核苷酸合成反应产率的组合物和方法
公开(公告)号：US20020055162A1
公开(公告)日：2002-05-09
申请号：US09966494
申请日：2001-09-28
申请人： Ambion, Inc.
发明人： Susan C. Milburn , Marianna Goldrick , Matthew Winkler
IPC分类号： C12N009/12 , C12N009/14 , C12P019/34
CPC分类号： C12P19/34
摘要： The present invention relates to compositions and methods for increasing the yields of polynucleotide synthetic reactions. In particular, it relates to an improved reaction mixture for use in in vitro RNA trancription and in various other enzymatic reactions in which a polynucleotide is synthesised. The reaction mixture uses high concentrations of total nucleotides, in the order of 12 mM to 40 mM, i.e. levels that were previously thought to be inhibitory. Other useful modifications are also disclosed.
摘要翻译：本发明涉及增加多核苷酸合成反应产率的组合物和方法。特别地，本发明涉及用于体外RNA转录和其中合成多核苷酸的各种其它酶反应中的改进的反应混合物。反应混合物使用高浓度的总核苷酸，大约12mM至40mM，即先前认为是抑制性的水平。还公开了其它有用的修饰。

5. 发明申请

US20010016312A1 Methods and reagents for preserving RNA in cell and tissue samples 有权
标题翻译：在细胞和组织样品中保存RNA的方法和试剂
公开(公告)号：US20010016312A1
公开(公告)日：2001-08-23
申请号：US09771256
申请日：2001-01-26
申请人： Ambion, Inc.
发明人： Eric S. Lader
IPC分类号： C07H021/02 , A01N001/02 , C07H021/00 , C07H021/04
CPC分类号： C12Q1/6806 , C12N1/04 , C12N15/1003
摘要： This specification relates to the field of molecular biology and provides novel methods and reagents for preserving and protecting the ribonucleic acid (RNA) content of samples from degradation prior to RNA isolation. This preservation may be accomplished without ultra-low temperature storage or disruption of the tissue.
摘要翻译：本说明书涉及分子生物学领域，并提供用于在RNA分离之前保存和保护样品的核糖核酸（RNA）含量降解的新方法和试剂。这种保存可以在没有超低温储存或组织破坏的情况下实现。

6. 发明申请

US20040230048A1 HIGH EFFICIENCY MRNA ISOLATION METHODS AND COMPOSITIONS 有权
标题翻译：高效MRNA分离方法和组合物
公开(公告)号：US20040230048A1
公开(公告)日：2004-11-18
申请号：US09854412
申请日：2001-05-11
申请人： AMBION, INC.
发明人： Richard C. Conrad
IPC分类号： C07H021/04
CPC分类号： C12N15/10
摘要： The present invention provides methods and compositions, including kits, for the isolation and purification of mRNA, particularly poly(A) RNA. It concerns the use of isostabilizing salts such as TMAC and TEAC to reduce rRNA carryover during the purification process, thus facilitating the isolation of poly(A) RNA.
摘要翻译：本发明提供了用于分离和纯化mRNA，特别是聚（A）RNA的方法和组合物，包括试剂盒。它涉及使用等稳化盐如TMAC和TEAC以在纯化过程中减少rRNA携带，从而促进聚（A）RNA的分离。

7. 发明申请

US20020177570A1 Compositions and methods for increasing the yields of in vitro RNA transcription and other polynucleotide synthetic reactions 失效
公开(公告)号：US20020177570A1
公开(公告)日：2002-11-28
申请号：US09966062
申请日：2001-09-28
申请人： Ambion, Inc.
发明人： Susan C. Milburn , Marianna Goldrick , Matthew Winkler
IPC分类号： A61K031/70 , A61K033/24 , A61K033/06 , A61K038/43
CPC分类号： C12P19/34
摘要： The present invention relates to compositions and methods for increasing the yields of polynucleotide synthetic reactions. In particular, it relates to an improved reaction mixture for use in in vitro RNA trancription and in various other enzymatic reactions in which a polynucleotide is synthesised. The reaction mixture uses high concentrations of total nucleotides, in the order of 12 mM to 40 mM, i.e. levels that were previously thought to be inhibitory. Other useful modifications are also disclosed.

8. 发明申请

US20020026046A1 Methods and reagents for inactivating ribonucleases 有权
标题翻译：灭活核糖核酸酶的方法和试剂
公开(公告)号：US20020026046A1
公开(公告)日：2002-02-28
申请号：US09815577
申请日：2001-03-23
申请人： AMBION, INC.
发明人： Brittan L. Pasloske , William Wu
IPC分类号： C07H021/02 , C12N009/99
CPC分类号： C12N9/22 , C12N9/99
摘要： The present invention is a general method for inactivating or inhibiting ribonucleases. Ribonucleases are treated with a reducing agent and heat. RNA samples contaminated with ribonuclease may be treated with this method to protect them from degradation. The RNA may then be used directly in a variety of enzymatic reactions and molecular biology techniques. This method may also be applied to a variety of molecular biology reagents which may be contaminated with ribonuclease to protect an RNA from being degraded when incubated with the reagent.
摘要翻译：本发明是灭活或抑制核糖核酸酶的一般方法。用还原剂和热处理核糖核酸酶。可以用这种方法处理被核糖核酸酶污染的RNA样品，以防止其降解。然后RNA可以直接用于各种酶反应和分子生物学技术。该方法也可以应用于可能被核糖核酸酶污染的各种分子生物学试剂，以保护RNA与试剂一起温育时不被降解。

9. 发明专利

AT481499T 未知
公开(公告)号：AT481499T
公开(公告)日：2010-10-15
申请号：AT00966924
申请日：2000-09-25
申请人： AMBION INC
发明人： WINKLER MATTHEW , KUDLICKI W , PASLOSKE BRITTAN
IPC分类号： C12Q1/34 , C07K16/40 , C12N9/00 , C12N15/10 , G01N33/573

10. 发明专利

DE69942351D1 Methoden und Reagenzien zur Erhaltung der RNA in Zellen und Geweben 未知
公开(公告)号：DE69942351D1
公开(公告)日：2010-06-17
申请号：DE69942351
申请日：1999-07-30
申请人： AMBION INC
发明人： LADER ERIC S
IPC分类号： C12Q1/68 , G01N33/50 , C12N1/04 , C12N15/09 , C12N15/10 , C12Q1/6806

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