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1. 发明申请

WO2009020528A1 VACUOLAR PYROPHOSPHATASES AND USES IN PLANTS 审中-公开
标题翻译： VACUOLAR PYROPHOSPHATASES和在植物中的用途
公开(公告)号：WO2009020528A1
公开(公告)日：2009-02-12
申请号：PCT/US2008/009091
申请日：2008-07-28
申请人： UNIVERSITY OF CONNECTICUT , WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH , BETH ISRAEL DEACONESS MEDICAL CENTER, INC. , GAXIOLA, Roberto, A. , FINK, Gerald, R. , ALPER, Seth, L.
发明人： GAXIOLA, Roberto, A. , FINK, Gerald, R. , ALPER, Seth, L.
IPC分类号： C12N15/82
CPC分类号： C12N15/8259 , C12N15/8261 , C12N15/8273 , Y02A40/146
摘要： The present invention relates to a transgenic plant which is tolerant to a salt, comprising one or more plant cells transformed with exogenous nucleic acid which alters expression of vacuolar pyrophosphatase in the plant. The present invention also relates to a transgenic plant with increased Pi uptake, comprising one or more plant cells transformed with exogenous nucleic acid which alters expression of vacuolar pyrophosphatase in the plant. Also encompassed by the present invention are transgenic progeny and seeds of the transgenic plants described herein. Progeny transgenic plant grown from seed are also described. Plant cells (e.g., root cells, stem cell, leaf cells, flower cells, fruit cells and seed cells) comprising exogenous nucleic acid which alters expression of vacuolar pyrophosphatase in the plant cell are also the subject of the present invention. Also encompassed by the present invention are methods of making a transgenic plant described herein. Transgenic plants produced by the methods of making a transgenic plant as described herein are also a subject of the present invention. The present invention also relates to a method of bioremediating soil, a method of increasing the yield of a plant, a method of making a plant which is larger than its corresponding wild type plant, a method of producing a transgenic plant which grows in salt water, and a method of producing a transgenic plant with increased Pi uptake. The transgenic plants of the present invention can also be used to produce double transgenic plants which are tolerant to a salt, or have increased Pi uptake.
摘要翻译：本发明涉及对盐具有耐受性的转基因植物，其包含用外源核酸转化的一种或多种植物细胞，其改变植物中液泡焦磷酸酶的表达。本发明还涉及具有增加的Pi摄取的转基因植物，其包含用外源核酸转化的一种或多种植物细胞，其改变植物中液泡焦磷酸酶的表达。本发明还包括本文所述的转基因植物的转基因后代和种子。还描述了从种子生长的后代转基因植物。包含改变植物细胞中液泡焦磷酸酶表达的外源核酸的植物细胞（例如根细胞，干细胞，叶细胞，花细胞，果实细胞和种子细胞）也是本发明的主题。本发明还包括制备本文所述的转基因植物的方法。通过制备如本文所述的转基因植物的方法产生的转基因植物也是本发明的主题。本发明还涉及一种生物中和土壤的方法，提高植物产量的方法，制备大于其相应野生型植物的植物的方法，生产在盐水中生长的转基因植物的方法，以及生产具有增加的Pi摄取的转基因植物的方法。本发明的转基因植物也可以用于产生耐受盐或具有增加的Pi吸收的双转基因植物。

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