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    • 5. 发明申请
    • GRAFTED CROSS-LINKED POLYOLEFIN SUBSTRATES FOR PEPTIDE SYNTHESIS AND ASSAYS
    • 用于肽合成和测定的交联的交联聚烯烃基材
    • WO1995000533A1
    • 1995-01-05
    • PCT/DK1994000245
    • 1994-06-20
    • FORSKNINGSCENTER RISOBATSBERG PEDERSEN, WalterALMDAL, KristofferWINTHER, LarsBERG, Rolf, Henrik
    • FORSKNINGSCENTER RISO
    • C07K01/04
    • G01N33/545C07K1/045C08F8/00C08F255/02Y10S977/736
    • A solid support for the solid-phase synthesis of peptides or proteins in high yield and in high purity, suited both to the synthesis of a single peptide or protein and to the parallel and substantially simultaneous synthesis of a plurality thereof, is based on a cross-linked polyolefin, especially polyethylene, substrate, the cross-linking having been obtained by irradiation with high energy electrons or gamma radiation or treatment with organic peroxide such as benzoyl peroxide, to which substrate are grafted polymer chains such as polystyrene chains which are functionalized with a chemical functionality facilitating the formation of an anchoring linkage between the polymer moiety and another chemical species. The solid support is also suited for use in solid-phase biosystems, notably bioassays, such as immunoassays, DNA hybridization assays or PCR amplification. The grafted chains may bear substituents which are such that the polymer-grafted cross-linked polyolefin substrate is swellable by water or aqueous media, in other words, hydrophilic, which makes the solid support particularly well suited for assays of the ELISA type.
    • 以高产率和高纯度固相合成肽或蛋白质的固相支持,适用于单一肽或蛋白质的合成以及其并行和基本上同时合成多个肽的基础是基于交叉 连接的聚烯烃,特别是聚乙烯,底物,通过用高能电子或γ辐射照射或用有机过氧化物如过氧化苯甲酰处理获得的交联,其中底物是接枝聚合物链的聚合物链,例如聚苯乙烯链,其被官能化 有助于在聚合物部分和另一种化学物质之间形成锚定连接的化学功能。 固体支持物也适用于固相生物系统,特别是生物测定,如免疫测定,DNA杂交测定或PCR扩增。 接枝的链可以带有取代基,使得聚合物接枝的交联聚烯烃底物可以通过水或水性介质溶胀,换句话说是亲水的,这使得固体支持物特别适合于ELISA类型的测定。