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    • 5. 发明申请
    • MEANS AND METHODS FOR CYTOMETRIC THERAPIES
    • 细胞治疗方法和方法
    • WO2008057370A2
    • 2008-05-15
    • PCT/US2007/023047
    • 2007-11-01
    • UT-BATTELLE, LLCVIRGINIA COMMONWEALTH UNIVERSITY INTELLECTUAL PROPERTY FOUNDATIONUNIVERSITY OF VIRGINIA PATENT FOUNDATIONGILLIES, George, T.FILLMORE, HelenBROADDUS, William, C.EVANS, Boyd, M.ALLISON, Stephen, W.
    • GILLIES, George, T.FILLMORE, HelenBROADDUS, William, C.EVANS, Boyd, M.ALLISON, Stephen, W.
    • A61K49/00
    • A61B5/0084A61B5/0071A61B5/0075
    • A functionalized tip is incorporated into catheters for the cytometric delivery of cells into the brain and other body parts. For use in the brain, the tip forms part of a neurosurgical probe having a proximal end and a distal end. In addition to the functionalized tip, the probe has at least one cell slurry delivery lumen and a plurality of optical fibers configured along the probe, terminating in the tip to provide the photo-optical capability needed to monitor the viability and physiological behavior of the grafted cells as well as certain characteristics of the cellular environment. Several specific embodiments of the invention are described, as are methods for use of it within the context of neurosurgically-based cell implantation, as one example of clinical application. Details are also presented of the use of a neurocatheter having a cytometric tip of the type disclosed in the invention, as employed within the context of a feedback and control system for regulating the number of cells delivered to the brain of a patient. The invention is further directed to cytometric determinations of cell viability during the delivery of cells into the brain and other target organs or body parts are disclosed.
    • 一个功能化的尖端被并入到导管中,用于将细胞细胞传递到脑和其他身体部位。 为了在脑中使用,尖端形成具有近端和远端的神经外科探针的一部分。 除了功能化的尖端之外,探针具有至少一个细胞浆料递送管腔和沿着探针配置的多个光纤,终止在尖端中以提供监测嫁接的生存力和生理行为所需的光学光学能力 细胞以及细胞环境的某些特征。 作为临床应用的一个实例,还描述了本发明的几个具体实施方案,以及在神经外科手术细胞植入的背景下使用本发明的方法。 还提供了在用于调节递送到患者脑部的细胞数量的反馈和控制系统的上下文中使用的具有本发明公开的类型的细胞计数尖端的神经导管的用途的细节。 本发明进一步涉及在细胞递送到脑中的细胞活力的细胞计数测定,并且公开了其它目标器官或身体部位。