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1. 发明申请

WO2016059474A3 SEQUENCE CONVERSION AND SIGNAL AMPLIFIER DNA HAVING POLY DNA SPACER SEQUENCES AND DETECTION METHODS USING SAME 审中-公开
公开(公告)号：WO2016059474A3
公开(公告)日：2016-04-21
申请号：PCT/IB2015/002145
申请日：2015-10-13
申请人： ABBOTT JAPAN CO., LTD , TOKYO INSTITUTE OF TECHNOLOGY
发明人： KOMIYA, Ken , KOMORI, Makoto , YOSHIMURA, Toru
IPC分类号： C12Q1/68 , C12Q1/34 , C12Q1/48
摘要： Disclosed are methods for detecting a target nucleic acid in a sample. The methods include contacting said sample, in the presence of a polymerase and an endonuclease, with a sequence conversion oligonucleotide. Also disclosed are methods for detecting a target nucleic acid in a sample in which said sample is contacted, in the presence of a polymerase and an endonuclease, with a sequence conversion oligonucleotide and a signal amplifier oligonucleotide. The disclosure also provides compositions and kits comprising such sequence conversion and signal amplifier oligonucleotides.

2. 发明申请

WO2015114469A3 COVERED SEQUENCE CONVERSION DNA AND DETECTION METHODS 审中-公开
标题翻译：覆盖序列转换DNA和检测方法
公开(公告)号：WO2015114469A3
公开(公告)日：2015-11-26
申请号：PCT/IB2015000726
申请日：2015-01-15
申请人： ABBOTT JAPAN CO LTD , TOKYO INST TECH
发明人： KOMIYA KEN , KOMORI MAKOTO , YOSHIMURA TORU
IPC分类号： C12Q1/68
CPC分类号： C12Q1/707 , C12Q1/682 , C12Q1/6844 , C12Q1/689 , C12Q1/703 , C12Q1/706 , C12Q2600/158 , C12Q2600/178 , C12Q2521/301 , C12Q2525/301
摘要： Disclosed are methods for detecting a target nucleic acid in sample, which include contacting the sample with an oligonucleotide having a hairpin structure, where the oligonucleotide includes, in the 5' to 3' direction, an arbitrary sequence, an endonuclease recognition site for a nicking reaction, a sequence complementary to the arbitrary sequence, and a sequence complementary to the target nucleic acid; a polymerase; and an endonuclease capable of nicking the endonuclease recognition site. The disclosure also provides compositions and kits comprising an oligonucleotide having a hairpin structure, where the oligonucleotide includes, in the 5' to 3' direction an arbitrary sequence, an endonuclease recognition site for a nicking reaction, a sequence complementary to the arbitrary sequence, and a sequence complementary to a target nucleic acid.
摘要翻译：公开了用于检测样品中的靶核酸的方法，其包括使样品与具有发夹结构的寡核苷酸接触，其中所述寡核苷酸在5'至3'方向上包括任意序列，用于切割的核酸内切酶识别位点反应，与任意序列互补的序列和与靶核酸互补的序列; 聚合酶; 和能够切割内切核酸酶识别位点的内切核酸酶。本公开还提供了包含具有发夹结构的寡核苷酸的组合物和试剂盒，其中所述寡核苷酸在5'至3'方向上包括任意序列，切口反应的核酸内切酶识别位点，与所述任意序列互补的序列和与靶核酸互补的序列。

3. 发明申请

WO2016059474A2 SEQUENCE CONVERSION AND SIGNAL AMPLIFIER DNA HAVING POLY DNA SPACER SEQUENCES AND DETECTION METHODS USING SAME 审中-公开
标题翻译：具有多聚DNA间隔序列的序列转换和信号扩增DNA以及使用相同的检测方法
公开(公告)号：WO2016059474A2
公开(公告)日：2016-04-21
申请号：PCT/IB2015002145
申请日：2015-10-13
申请人： ABBOTT JAPAN CO LTD , TOKYO INST TECH
发明人： KOMIYA KEN , KOMORI MAKOTO , YOSHIMURA TORU
IPC分类号： C12Q1/68 , C12Q1/34 , C12Q1/48
CPC分类号： C12Q1/682 , C12Q1/6844 , C12Q2525/113 , C12Q2525/131 , C12Q2531/119
摘要： Disclosed are methods for detecting a target nucleic acid in a sample. The methods include contacting said sample, in the presence of a polymerase and an endonuclease, with a sequence conversion oligonucleotide. Also disclosed are methods for detecting a target nucleic acid in a sample in which said sample is contacted, in the presence of a polymerase and an endonuclease, with a sequence conversion oligonucleotide and a signal amplifier oligonucleotide. The disclosure also provides compositions and kits comprising such sequence conversion and signal amplifier oligonucleotides.
摘要翻译：公开了用于检测样品中的靶核酸的方法。所述方法包括使所述样品在聚合酶和内切核酸酶的存在下与序列转化寡核苷酸接触。还公开了在聚合酶和内切核酸酶的存在下用序列转换寡核苷酸和信号放大寡核苷酸检测其中所述样品接触的样品中的靶核酸的方法。本公开还提供了包含这种序列转换和信号放大寡核苷酸的组合物和试剂盒。

4. 发明申请

WO2018143478A1 METHOD FOR REDUCING NOISE IN A SIGNAL-GENERATING DIGITAL ASSAYS 审中-公开
公开(公告)号：WO2018143478A1
公开(公告)日：2018-08-09
申请号：PCT/JP2018/004089
申请日：2018-02-06
申请人： ABBOTT JAPAN CO., LTD.
发明人： NAKAI FUTASE Atsuko
IPC分类号： G01N33/543
摘要： Methods of reducing background noise in signal-generating digital assays, such as fluorescent digital immunoassays, using a colorant are disclosed. The methods utilize a binding member that specifically binds to an analyte in a biological sample. The binding member is conjugated to a signal generating compound, e.g., an enzyme, which is dissociated from the binding member. A colorant is added with a signal generating substrate, e.g., a fluorogenic or chromogenic substrate for the enzyme, or after a signal generating substrate to reduce background noise in a fluorescent digital immunoassays. The signal generated between the signal generating compound and the signal generating substrate is detected and correlated to the presence and/or concentration of the analyte.

5. 发明申请

WO2017057778A2 METHODS OF DIAGNOSING HEPATOCELLULAR CARCINOMA AND PANCREATIC CANCER 审中-公开
标题翻译：诊断中肝癌和胰腺癌方法
公开(公告)号：WO2017057778A2
公开(公告)日：2017-04-06
申请号：PCT/JP2016/079711
申请日：2016-09-28
申请人： ABBOTT JAPAN CO., LTD. , THE UNIVERSITY OF TOKYO
发明人： YOSHIMURA, Toru , YOSHIDA, Eisaku , KOSHIKAWA, Naohiko , SEIKI, Motoharu
IPC分类号： G01N33/574
CPC分类号： G01N33/57438 , G01N2333/78 , G01N2800/50 , G01N2800/56
摘要： Disclosed herein are biomarkers for hepatocellular carcinoma and pancreatic cancer. The biomarkers may be laminin gamma 2 monomer, PIVKA-II, AFP, CEA, CA19-9, or combinations thereof. Also disclosed herein are methods of diagnosing, prognosing, classifying risk, and monitoring progression of hepatocellular carcinoma or pancreatic cancer by the detecting the level of laminin gamma 2 monomer, PIVKA-II, AFP, CEA, CA19-9, or combinations thereof.
摘要翻译：本文公开了用于肝细胞癌和胰腺癌的生物标志物。生物标记可以是层粘连蛋白的γ2单体，PIVKA-II，AFP，CEA，CA19-9，或它们的组合。本文还公开了诊断，预后，分类风险，并且由所述检测层粘连蛋白伽玛2单体，PIVKA-II，AFP，CEA，CA19-9，或它们的组合的水平监测肝细胞癌或胰腺癌的进展的方法。

6. 发明申请

WO2016103234A1 DETECTION METHOD BASED ON SIGNAL AMPLIFIER DNA CASCADE REACTIONS WITH TARGET EXTENSION 审中-公开
标题翻译：基于信号放大器的DNA检测方法与目标扩展的反应
公开(公告)号：WO2016103234A1
公开(公告)日：2016-06-30
申请号：PCT/IB2015/059986
申请日：2015-12-24
申请人： ABBOTT JAPAN CO., LTD.
发明人： MAKOTO, Komori , TORU, Yoshimura
IPC分类号： C12Q1/68
CPC分类号： C12Q1/682 , C12Q1/6844 , C12Q1/6886 , C12Q2600/178 , C12Q2521/301 , C12Q2537/1373 , C12Q2537/149 , C12Q2525/131 , C12Q2525/186 , C12Q2525/301 , C12Q2525/207 , C12Q2537/137
摘要： Methods, compositions and kits for detecting a target nucleic acid in a sample. The methods include contacting the sample, in the presence of a polymerase and an endonuclease, with a first oligonucleotide comprising, in the 5' to 3' direction, a first signal DNA generation sequence, an endonuclease recognition site, and a sequence complementary to the 3' end of a target nucleic acid; a second oligonucleotide comprising, in the 5' to 3' direction, a second signal DNA generation sequence, an endonuclease recognition site, and a sequence that is homologous to the first signal DNA generation sequence of the first oligonucleotide; a third oligonucleotide comprising, in the 5' to 3' direction, a third signal DNA generation sequence, an endonuclease recognition site, and a sequence that is homologous to the second signal DNA generation sequence of the second oligonucleotide. The reaction causes a signal cascade. The polymerase preferably has strand displacement activity and the target nucleic acid is preferably a microRNA.
摘要翻译：用于检测样品中靶核酸的方法，组合物和试剂盒。所述方法包括在聚合酶和内切核酸酶的存在下使样品与第一寡核苷酸接触，所述第一寡核苷酸在5'至3'方向上包含第一信号DNA产生序列，内切核酸酶识别位点和与 3'末端; 第二寡核苷酸，其在5'至3'方向上包含第二信号DNA产生序列，内切核酸酶识别位点和与第一寡核苷酸的第一信号DNA产生序列同源的序列; 第三寡核苷酸，其在5'至3'方向上包含第三信号DNA产生序列，内切核酸酶识别位点和与第二寡核苷酸的第二信号DNA产生序列同源的序列。反应会引起信号级联。聚合酶优选具有链置换活性，靶核酸优选为微RNA。

7. 发明申请

WO2016059473A3 SEQUENCE CONVERSION AND SIGNAL AMPLIFIER DNA HAVING LOCKED NUCLEIC ACIDS AND DETECTION METHODS USING SAME 审中-公开
标题翻译：具有锁定核酸的序列转换和信号放大DNA及其使用的检测方法
公开(公告)号：WO2016059473A3
公开(公告)日：2016-06-23
申请号：PCT/IB2015002141
申请日：2015-10-13
申请人： ABBOTT JAPAN CO LTD , TOKYO INST TECH
发明人： KOMIYA KEN , KOMORI MAKOTO , TORU YOSHIMURA
IPC分类号： C12Q1/68 , C12N15/00
CPC分类号： C12Q1/682 , C12Q1/6844 , C12Q2525/113 , C12Q2525/131 , C12Q2531/119
摘要： Disclosed are methods for detecting a target nucleic acid in a sample. The methods include contacting said sample, in the presence of a polymerase and an endonuclease, with a sequence conversion oligonucleotide having locked nucleic acids at select positions sufficient to decrease non-specific background signal amplification. Also disclosed are methods for detecting a target nucleic acid in a sample in which said sample is contacted, in the presence of a polymerase and an endonuclease, with a sequence conversion oligonucleotide and a signal amplifier oligonucleotide, both having locked nucleic acids at select positions sufficient to decrease non-specific background signal amplification. The disclosure also provides compositions and kits comprising such sequence conversion and signal amplifier oligonucleotides.
摘要翻译：公开了用于检测样品中靶核酸的方法。所述方法包括在聚合酶和内切核酸酶的存在下使所述样品与在足以减少非特异性背景信号扩增的选择位置处具有锁定核酸的序列转化寡核苷酸接触。还公开了用于在聚合酶和内切核酸酶的存在下与序列转化寡核苷酸和信号放大寡核苷酸接触的样品中的靶核酸的检测方法，所述序列转化寡核苷酸和信号放大寡核苷酸都在足够的选择位置具有锁定的核酸以减少非特异性背景信号放大。本公开还提供了包含这种序列转化和信号放大器寡核苷酸的组合物和试剂盒。

8. 发明申请

WO2016059473A2 SEQUENCE CONVERSION AND SIGNAL AMPLIFIER DNA HAVING LOCKED NUCLEIC ACIDS AND DETECTION METHODS USING SAME 审中-公开
标题翻译：具有锁定核酸的序列转换和信号放大器DNA以及使用相同的检测方法
公开(公告)号：WO2016059473A2
公开(公告)日：2016-04-21
申请号：PCT/IB2015/002141
申请日：2015-10-13
申请人： ABBOTT JAPAN CO., LTD , TOKYO INSTITUTE OF TECHNOLOGY
发明人： KOMIYA, Ken , KOMORI, Makoto , TORU, Yoshimura
IPC分类号： C12Q1/68
CPC分类号： C12Q1/682 , C12Q1/6844 , C12Q2525/113 , C12Q2525/131 , C12Q2525/185 , C12Q2531/119
摘要： Disclosed are methods for detecting a target nucleic acid in a sample. The methods include contacting said sample, in the presence of a polymerase and an endonuclease, with a sequence conversion oligonucleotide having locked nucleic acids at select positions sufficient to decrease non-specific background signal amplification. Also disclosed are methods for detecting a target nucleic acid in a sample in which said sample is contacted, in the presence of a polymerase and an endonuclease, with a sequence conversion oligonucleotide and a signal amplifier oligonucleotide, both having locked nucleic acids at select positions sufficient to decrease non-specific background signal amplification. The disclosure also provides compositions and kits comprising such sequence conversion and signal amplifier oligonucleotides.
摘要翻译：公开了用于检测样品中的靶核酸的方法。所述方法包括使所述样品在聚合酶和内切核酸酶的存在下与序列转换寡核苷酸接触，所述序列转换寡核苷酸在足以降低非特异性背景信号扩增的选择位置处具有锁核酸。还公开了在聚合酶和内切核酸酶存在下，用序列转换寡核苷酸和信号放大寡核苷酸检测其中所述样品接触的样品中的靶核酸的方法，所述序列转换寡核苷酸和信号放大寡核苷酸均在选择位置处具有锁定核酸减少非特异性背景信号放大。本公开还提供了包含这种序列转换和信号放大寡核苷酸的组合物和试剂盒。

9. 发明申请

WO2015114469A2 COVERED SEQUENCE CONVERSION DNA AND DETECTION METHODS 审中-公开
标题翻译：覆盖序列转化DNA和检测方法
公开(公告)号：WO2015114469A2
公开(公告)日：2015-08-06
申请号：PCT/IB2015/000726
申请日：2015-01-15
申请人： ABBOTT JAPAN CO., LTD , TOKYO INSTITUTE OF TECHNOLOGY
发明人： KOMIYA, Ken , KOMORI, Makoto , YOSHIMURA, Toru
CPC分类号： C12Q1/707 , C12Q1/682 , C12Q1/6844 , C12Q1/689 , C12Q1/703 , C12Q1/706 , C12Q2600/158 , C12Q2600/178 , C12Q2521/301 , C12Q2525/301
摘要： Disclosed are methods for detecting a target nucleic acid in sample, which include contacting the sample with an oligonucleotide having a hairpin structure, where the oligonucleotide includes, in the 5' to 3' direction, an arbitrary sequence, an endonuclease recognition site for a nicking reaction, a sequence complementary to the arbitrary sequence, and a sequence complementary to the target nucleic acid; a polymerase; and an endonuclease capable of nicking the endonuclease recognition site. The disclosure also provides compositions and kits comprising an oligonucleotide having a hairpin structure, where the oligonucleotide includes, in the 5' to 3' direction an arbitrary sequence, an endonuclease recognition site for a nicking reaction, a sequence complementary to the arbitrary sequence, and a sequence complementary to a target nucleic acid.
摘要翻译：公开了用于检测样品中靶核酸的方法，其包括使样品与具有发夹结构的寡核苷酸接触，其中寡核苷酸在5'至3'方向上包含任意序列，用于切口的内切核酸酶识别位点反应，与任意序列互补的序列和与靶核酸互补的序列; 聚合酶和能够切割内切核酸酶识别位点的核酸内切酶。本公开还提供了包含具有发夹结构的寡核苷酸的组合物和试剂盒，其中寡核苷酸在5'至3'方向上包含任意序列，用于切口反应的内切核酸酶识别位点，与任意序列互补的序列，以及与靶核酸互补的序列。

10. 发明申请

WO2012161288A1 IMMUNOASSAY METHODS AND REAGENTS FOR DECREASING NONSPECIFIC BINDING 审中-公开
标题翻译：用于减少非特异性结合的免疫测定方法和试剂
公开(公告)号：WO2012161288A1
公开(公告)日：2012-11-29
申请号：PCT/JP2012063398
申请日：2012-05-15
申请人： ABBOTT JAPAN CO LTD , YOSHIMURA TARU , CHIBA RYOTARO , YOSHIDA EISAKU
发明人： YOSHIMURA TARU , CHIBA RYOTARO , YOSHIDA EISAKU
IPC分类号： G01N33/543
CPC分类号： G01N33/86
摘要： Methods and kits for reducing non-specific binding in an immunoassay for PIVKA-II in a test sample are described, in which the test sample is reacted with an anti-prothrombin antibody in the presence of one or more of the following additives: skim milk, saponin, CaCl2, MgCl2, and a sulfobetaine zwitterionic detergent.
摘要翻译：描述了在测试样品中用于降低PIVKA-II的免疫测定中的非特异性结合的方法和试剂盒，其中所述测试样品在一种或多种以下添加剂存在下与抗凝血酶原抗体反应：脱脂乳，皂角苷，CaCl 2，MgCl 2和磺基甜菜碱两性离子洗涤剂。

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