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    • 1. 发明申请
    • METHOD OF CONVERTING CARBON DIOXIDE AND HYDROGEN TO METHANE BY MICROORGANISMS
    • 通过微生物将二氧化碳和氢转化成甲烷的方法
    • WO2012110256A1
    • 2012-08-23
    • PCT/EP2012/000725
    • 2012-02-17
    • KRAJETE, AlexanderKrajete GmbH
    • KRAJETE, Alexander
    • C12P5/02C12M1/00C12R1/00
    • C12M21/04C12M29/20C12M41/26C12M41/28C12M41/40C12N1/20C12P5/023Y02E50/343
    • The invention provides a method of converting hydrogen and carbon dioxide into methane by methanogenic microorganisms in a reaction vessel, comprising contacting the methanogenic microorganisms with in-gas comprising hydrogen and carbon dioxide. The method comprises at least one methane production phase, wherein in the at least one methane production phase the ratio of the partial pressure of hydrogen to the partial pressure of carbon dioxide inside the reaction vessel is adjusted to 5:1 or higher (parts hydrogen : parts carbon dioxide). The method of the invention may optionally comprise at least one growth phase, wherein in the at least one cell growth phase the ratio of the partial pressure of hydrogen to the partial pressure of carbon dioxide inside the reaction vessel is adjusted to a ratio different from the ratio in the at least one methane production phase. The invention also provides a bioreactor suitable for the method of the invention of converting hydrogen and carbon dioxide into methane by methanogenic microorganisms. In addition, methanogenic microorganisms are provided which are having a biomass concentration of at least 6 g biomass per litre, wherein no additional methanogenic microorganism is added after inoculation or cell retention is applied during fermentation, and also the use of methanogenic microorganisms having a biomass concentration of at least 6 g biomass per litre under continuous fermentation conditions is provided.
    • 本发明提供了一种通过反应容器中的产甲烷微生物将氢气和二氧化碳转化为甲烷的方法,其中包括使生成甲烷的微生物与包含氢气和二氧化碳的气体接触。 该方法包括至少一个甲烷生产阶段,其中在至少一个甲烷生产阶段中,将氢的分压与反应容器内的二氧化碳分压的比率调节至5:1或更高(部分氢: 二氧化碳)。 本发明的方法可以任选地包括至少一个生长阶段,其中在至少一个细胞生长期中,将氢的分压与反应容器内的二氧化碳分压的比率调整到与 至少一个甲烷生产阶段的比例。 本发明还提供了适用于本发明的方法的生物反应器,其通过产甲烷微生物将氢气和二氧化碳转化为甲烷。 此外,提供了具有每升至少6g生物量的生物质浓度的产甲烷微生物,其中在发酵过程中在接种或细胞保留之后不再添加额外的产甲烷微生物,并且还使用具有生物质浓度的产甲烷微生物 提供了在连续发酵条件下每升至少6g生物量。