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    • 3. 发明申请
    • POLYPEPTIDES WITH PERMEASE ACTIVITY
    • 具有过氧化物酶活性的多糖
    • WO2014195520A2
    • 2014-12-11
    • PCT/EP2014/063068
    • 2014-06-20
    • DSM IP ASSETS B.V.RIJKSUNIVERSITEIT GRONINGEN
    • KLAASSEN, PaulDE WAAL, Paulus PetrusDE JONG, René MarcelDRIESSEN, Arnold Jacob MattieuNIJLAND, Jeroen GerbenSHIN, Hyun Yong
    • C07K14/395
    • C07K14/395
    • The invention relates to a polypeptide having one or more substitution corresponding to F85T/A/C/H/M/N, T89H/D/C/A/N, V187T/C/D/E/F/G/H/K/M/Q/R/S/W/Y, A191N/G/P/ C/D/F/H/I/K/Q/V/W/Y, Y214V/Q/G/L/M/N/P/R/S, Q215L/M/I/A/C/D/F/S, I218K/N/A/C/D/E/H/L, T219G/F/D/N, I222D/C/H/E/G/P/S/V/Y, Y226D/M/R/C/G/H/I/L/P/Q/T/W, Q338C/E/F/D/G/I/P/R/S/T/V/W/Y, M339N/V/D/E/H/K/P/Q/R/Y, Q341T/M/N/S/Y, Q342Y/S/C/A/D/F/G/H/I/K/L/N/P/R/T/V/W, L343K/R/P/C/D/E/G/H/I/M/N/Q/S/T/Y, N346W/Y/F/G/H/K/L/M/R/T, N347A/I/E/H/L, Y350R/L/T/D/K/M/N/V, G373L/E/N /F/K/M/R/V/W/Y, N376I/T/M/A/C/E/F/H/K/L/P/Q/R/V/W/Y, T380M/F/H/N/P/Q/R/S/V, S383Q/T/C/D/E/F/I/K/L/M/R/V/W/Y, F444Y/D/E/H/K/N/P/Q/R/W, F446Q/L/H/D/E/I/M/N, T448M/H/D/E/F/K/R/W/Y, T449F/M/N/S/D/E/G/H/I/K/L/P/Q/R/V/W/Y, T451I/E/C/D/F/H/K/L/M/N/Q/R/S/T/V/W/Y, W455T/N/A/C/E/L/P/Q/T, N478P/F/V/G/I/K/R/W, or W479T/S/Q/D/E/F/G/H/I/M/N/P/R/V/Y of SEQ ID NO:59, wherein the polypeptide is a member of the Major Facilitator Superfamily (MFS).
    • 本发明涉及一种对F85T / A / C / H / M / N,T89H / D / C / A / N,V187T / C / D / E / F / G / H / K / M / Q / R / S / W / Y,A191N / G / P / C / D / F / H / I / K / Q / V / W / Y,Y214V / Q / G / L / M / N / P / R / S,Q215L / M / I / A / C / D / F / S,I218K / N / A / C / D / E / H / L,T219G / F / D / N,I222D / C / H / E / G / P / S / V / Y,Y226D / M / R / C / G / H / I / L / P / Q / T / W,Q338C / E / F / D / G / / P / R / S / T / V / W / Y,M339N / V / D / E / H / K / P / Q / R / Y,Q341T / M / N / S / Y,Q342Y / S / C / A / D / F / G / H / I / K / L / N / P / R / T / V / W,L343K / R / P / C / D / E / G / H / I / M / N / Q / S / T / Y,N346W / Y / F / G / H / K / L / M / R / T,N347A / I / E / H / L,Y350R / L / T / D / K / M / N / V,G373L / E / N / F / K / M / R / V / W / Y,N376I / T / M / A / C / E / F / H / K / L / P / Q / R / V / W / Y,T380M / F / H / N / P / Q / R / S / V,S383Q / T / C / D / E / F / I / K / L / M / R / V / W / Y,F444Y / D / E / H / K / N / P / Q / R / W,F446Q / L / H / D / E / I / M / N,T448M / H / D / E / F / K / R / W / Y,T449F / M / N / S / D / E / G / H / I / K / L / P / Q / R / V / W / Y,T451I / E / C / D / F / H / K / L / M / N / Q / R / S / T / V / W / Y,W455T / N / A / C / E / L / P / Q / T,N478P / F / V / G / I / K / R / W,或SEQ ID NO:59的W479T / S / Q / D / E / F / G / H / I / M / N / P / R / V / Y, 主要辅导员超家庭成员(MFS)的成员。
    • 4. 发明申请
    • POLYPEPTIDES WITH PERMEASE ACTIVITY
    • 具有过氧化物酶活性的多糖
    • WO2014195379A1
    • 2014-12-11
    • PCT/EP2014/061637
    • 2014-06-04
    • DSM IP ASSETS B.V.
    • KLAASSEN, PaulDE WAAL, Paulus PetrusDE JONG, René Marcel
    • C07K14/395
    • C07K14/395C12P7/10C12P19/02C12P19/14C12P2203/00Y02E50/16
    • The invention relates to a polypeptide having one or more substitution corresponding to T89C/Y, V187M/Q/C/G/Y, A191 l/K/D/C/E/N/W/R/P, Y214L/P, Q215D/E/S/M/L/C/F/A, 1218W, T219V/L/R/E, I222G/Y/M/P/S/W, Y226M/T/P/Q, Q338R/I/D/E/F/G/P, M339Y/E/N, Q341V/Y/S/H, Q342N/H/R/C/F/G/I/K/L/P/S/T/W, L343Y/Q/I/C/E/K/N/P/S, N346G/I, N347S/L/K/G/TA/, Y350E/S/Q, G373R/K/L/IWY, N376K/Q/A/C/D/E/I/M/P, T380Y/G/M/A/S/D/E/I/K/L/W, S383Y/WA//C/E/I/L/Q/R, F446H/D/I, T448/R/K/H/D/E/W, T449F/H/A/G/I/L/P/QA/, T451W/Y/S, W455D/K/E/P/Q, N478Q/I/F/G, or W479S/I/F/D/E/H/M/N/R/T/Y of SEQ ID NO:59 wherein the polypeptide is member of the Major Facilitator Superfamily (MFS).
    • 本发明涉及对应于T89C / Y,V187M / Q / C / G / Y,A191I / K / D / C / E / N / W / R / P,Y214L / P, Q215D / E / S / M / L / C / F / A,1218W,T219V / L / R / E,I222G / Y / M / P / S / W,Y226M / T / P / Q,Q338R / I / D / E / F / G / P,M339Y / E / N,Q341V / Y / S / H,Q342N / H / R / C / F / G / I / K / L / P / S / T / L343Y / Q / I / C / E / K / N / P / S,N346G / I,N347S / L / K / G / TA /,Y350E / S / Q,G373R / K / L / IWY,N376K / Q / A / C / D / E / I / M / P,T380Y / G / M / A / S / D / E / I / K / L / W,S383Y / WA // C / E / I / Q / R,F446H / D / I,T448 / R / K / H / D / E / W,T449F / H / A / G / I / L / P / QA /,T451W / Y / S,W455D / K / E / P / Q,N478Q / I / F / G或SEQ ID NO:59的W479S / I / F / D / E / H / M / N / R / T / Y,其中多肽是 主要辅导员超家庭(MFS)。
    • 5. 发明申请
    • PHYTASE IN BIOGAS PRODUCTION
    • 生物生产中的PHYTASE
    • WO2014001349A1
    • 2014-01-03
    • PCT/EP2013/063306
    • 2013-06-25
    • DSM IP ASSETS B.V.
    • STREEKSTRA, HugoVERLEUN, Theodorus Marius
    • C12P5/02C12N9/16C12P7/10
    • C12P5/023C12N9/16C12P2201/00C12P2203/00C12Y301/03Y02E50/343
    • The invention relates to a process for the production of biogas from organic material comprising: treating said organic material with an enzyme composition comprising a phytase, preferably a phytase and a cellulase and/or hemicellulase, and digesting the enzyme treated organic material to form biogas, and further to the use of an enzyme composition comprising a phytase to increase the digestibility of proteins and polysaccharides by microbes present in a process suitable for the conversion of organic material into biogas; the use of an enzyme composition comprising a phytase to increase the availability of minerals in a process suitable for the conversion of organic material into biogas; and the use of an enzyme composition comprising a phytase to reduce precipitation of salts on hardware such as (metal) surfaces and in lines and pumps in a process suitable for the conversion of organic material into biogas. Adding phytase to a biogas process advantageously results in increased biogas production, a more robust biogas production process, and reduced batch-to-batch variation, and allows the use of mixtures of organic material.
    • 本发明涉及一种用于从有机材料生产沼气的方法,包括:用包含植酸酶,优选植酸酶和纤维素酶和/或半纤维素酶的酶组合物处理所述有机材料,并消化经酶处理的有机材料以形成沼气, 并且还涉及使用包含植酸酶的酶组合物以通过适于将有机材料转化为生物气体的方法中存在的微生物来增加蛋白质和多糖的消化率; 使用包含植酸酶的酶组合物在适于将有机材料转化为生物气体的方法中增加矿物质的可利用性; 以及使用包含植酸酶的酶组合物以在适于将有机材料转化为生物气体的方法中减少硬件(例如(金属)表面和管线和泵上的盐的沉淀)。 将植酸酶添加到沼气工艺中有利地导致增加的沼气生产,更强劲的沼气生产过程和减少的批次间变化,并允许使用有机材料的混合物。