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1. 发明申请

WO2020132487A1 MODIFIED RHODAMINE DYE AND USE THEREOF IN BIOLOGICAL ASSAYS 审中-公开
公开(公告)号：WO2020132487A1
公开(公告)日：2020-06-25
申请号：PCT/US2019/067925
申请日：2019-12-20
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： EVANS, Brian , BENSON, Scott , MULLAH, Khairuzzaman Bashar , YAN, Xiongwei
IPC分类号： C09B11/24 , C07H21/00 , C12N15/65 , G01N33/58
摘要： The present invention is directed to f luorescent rhodamine dyes having spectral properties suited to the creation of multiplex assay systems for use in molecular biology, cell biology and molecular genetics. The rhodamine dyes have the following structure:

2. 发明申请

WO2016057951A3 CRISPR OLIGONUCLEOTIDES AND GENE EDITING 审中-公开
公开(公告)号：WO2016057951A3
公开(公告)日：2016-04-14
申请号：PCT/US2015/054986
申请日：2015-10-09
申请人： LIFE TECHNOLOGIES CORPORATION , THERMO FISHER SCIENTIFIC GENEART GMBH
发明人： RAVINDER, Namritha , HEIL, Korbinian , GUO, Yizhu , LIANG, Xiquan , POTTER, Robert
IPC分类号： C12Q1/68 , C12N15/113 , C12N15/10
摘要： The present disclosure generally relates to compositions and methods for the genetic modification of cells. In particular, the disclosure relates to CRISPR reagents and the use of such reagents.

3. 发明申请

WO2008112008A2 SENSOR PROTEINS AND ASSAY METHODS 审中-公开
标题翻译：传感器蛋白和测定方法
公开(公告)号：WO2008112008A2
公开(公告)日：2008-09-18
申请号：PCT/US2007/075705
申请日：2007-08-10
申请人： INVITROGEN CORPORATION , RIDDLE, Steven , NEWMAN, Rhonda , VOGEL, Kurt
发明人： RIDDLE, Steven , NEWMAN, Rhonda , VOGEL, Kurt
IPC分类号： C12N15/09 , G01N33/53 , C07H21/04 , C07K14/00
CPC分类号： G01N33/542 , G01N33/84 , Y10T436/16
摘要： The present invention relates to biosensors. In some embodiments, the biosensors are modified ligand binding molecules. In some embodiments, the modified ligand binding molecule is a phosphate binding protein (PBP). In some embodiments, the modified ligand binding molecules are labeled to be capable of RET, e.g. , comprising a donor and acceptor moiety. In some embodiments of the invention, there is a detectable change in RET ( e.g. , FRET) when the modified ligand binding molecule binds and/or releases the ligand ( e.g. , phosphate). The invention also provides related methods, reactions and assays.
摘要翻译：本发明涉及生物传感器。在一些实施方案中，生物传感器是修饰的配体结合分子。在一些实施方案中，修饰的配体结合分子是磷酸结合蛋白（PBP）。在一些实施方案中，修饰的配体结合分子被标记为能够包含供体和受体部分的RET（例如，例如）。在本发明的一些实施方案中，当修饰的配体结合分子结合和/或释放配体（例如磷酸盐）时，RET（例如FRET）中存在可检测的变化）。本发明还提供了相关的方法，反应和分析。

4. 发明申请

WO2016065364A1 COMPOSITIONS AND METHODS FOR ENHANCING HOMOLOGOUS RECOMBINATION 审中-公开
标题翻译：用于增强同源重建的组合物和方法
公开(公告)号：WO2016065364A1
公开(公告)日：2016-04-28
申请号：PCT/US2015/057401
申请日：2015-10-26
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： POTTER, Robert , CHESNUT, Jonathan , LIANG, Xiquan
IPC分类号： C12N15/90 , C12N9/22
CPC分类号： C12N9/22 , C07K2319/00 , C07K2319/81 , C12N15/902 , C12N2310/20
摘要： The present disclosure generally relates to compositions and methods for improving the efficiency of homologous recombination. In particular, the disclosure relates to reagents and the use of such reagents.
摘要翻译：本公开一般涉及用于提高同源重组效率的组合物和方法。特别地，本公开涉及试剂和这些试剂的用途。

5. 发明申请

WO2014153188A2 HIGH EFFICIENCY, SMALL VOLUME NUCLEIC ACID SYNTHESIS 审中-公开
标题翻译：高效率，小体积的核酸合成
公开(公告)号：WO2014153188A2
公开(公告)日：2014-09-25
申请号：PCT/US2014/029500
申请日：2014-03-14
申请人： LIFE TECHNOLOGIES CORPORATION , GENEART
发明人： PETERSON, Todd , KATZEN, Federico , LIANG, Xiquan , TREFZER, Axel , POEHMERER, Thomas
IPC分类号： C12N15/10
CPC分类号： C12P19/34 , C12N15/1031 , C12N15/1093
摘要： The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).
摘要翻译：本公开总体上涉及用于生产核酸分子的组合物和方法。在一些方面，本发明允许微量生成核酸分子，任选地随后将这些核酸分子组装成更大的分子。在一些方面，本发明允许有效产生核酸分子（例如大的核酸分子，例如基因组）。

6. 发明申请

WO2011066330A2 SELECTIVE AMPLIFICATION OF POLYNUCLEOTIDE SEQUENCES 审中-公开
标题翻译：多核苷酸序列的选择性扩增
公开(公告)号：WO2011066330A2
公开(公告)日：2011-06-03
申请号：PCT/US2010/057904
申请日：2010-11-23
申请人： LIFE TECHNOLOGIES CORPORATION , CHEN, Zhoutao , ZHANG, Hua
发明人： CHEN, Zhoutao , ZHANG, Hua
IPC分类号： C12Q1/68 , C12N15/10
CPC分类号： C12Q1/6846
摘要： The application relates to compositions and methods which may be used for amplifying selected portions of nucleic acid samples. Samples may comprise an entire genome of a bacterium, plant, animal, or other organism. In some instances, methods allow for enrichment of hundred or thousands of target nucleic acids of interest in an efficient and cost effective manner.
摘要翻译：本申请涉及可用于扩增核酸样品的选定部分的组合物和方法。样品可以包含细菌，植物，动物或其它生物体的整个基因组。在一些情况下，方法允许以有效和成本有效的方式富集数百或数千种感兴趣的靶核酸。

7. 发明申请

WO2009099967A1 SYSTEM AND METHOD FOR SELECTING SAMPLES FROM AN ANTIBODY ANALYSIS FOR DISPLAY OF COMPLETE SPECIFICITY ANALYSIS DATA 审中-公开
标题翻译：从用于显示完整特异性分析数据的抗体分析中选择样品的系统和方法
公开(公告)号：WO2009099967A1
公开(公告)日：2009-08-13
申请号：PCT/US2009/032723
申请日：2009-01-30
申请人： LIFE TECHNOLOGIES CORPORATION , LEAH, Samuel , CANTERBURY, Andrew , MCCOMBS, Erin , MUNROE, Donald
发明人： LEAH, Samuel , CANTERBURY, Andrew , MCCOMBS, Erin , MUNROE, Donald
IPC分类号： G06F19/00
CPC分类号： G06F19/18 , G06F19/26
摘要： A system and method provides for selecting one or more samples from an antibody specificity analysis for displaying complete antibody specificity analysis data. A plurality of sample sera are run through a protein panel for antibody analysis and a specificity analysis is completed. A positive or negative screening result is displayed for each sample within the panel, and for each positive sample, preliminary diagnostic data such as the PRA value and intensity value are provided. The preliminary diagnostic data is then reviewed by a user to determine whether complete specificity data of each positive sample is needed. In one embodiment, the complete specificity data is available for purchase on a sample-by-sample basis so that if only a small amount of samples are deemed useful, purchase of the complete specificity data for an entire panel of samples is avoided. The specificity data is encrypted until the user decides to purchase it.
摘要翻译：系统和方法提供从抗体特异性分析中选择一个或多个样品以显示完整的抗体特异性分析数据。多个样品血清通过蛋白质组进行抗体分析，并完成特异性分析。对于面板中的每个样品，显示正或负筛选结果，并且对于每个阳性样品，提供初步诊断数据，例如PRA值和强度值。然后由用户审查初步诊断数据，以确定是否需要每个阳性样品的完整特异性数据。在一个实施方案中，完整的特异性数据可以逐个样品购买，以便如果只有少量样品被认为是有用的，则避免购买整个样品片段的完整特异性数据。特殊性数据被加密直到用户决定购买它。

8. 发明申请

WO2008089396A1 COMPOSITIONS AND METHODS FOR GENETIC MANIPULATION AND MONITORING OF CELL LINES 审中-公开
标题翻译：用于细胞系的遗传操作和监测的组合物和方法
公开(公告)号：WO2008089396A1
公开(公告)日：2008-07-24
申请号：PCT/US2008/051417
申请日：2008-01-18
申请人： INVITROGEN CORPORATION , CHESNUT, Jonathan , BENNETT, Robert , BURRIER, Robert , LIEU, Pauline , RAO, Mahendra , TALIANA, Antje , THYAGARAJAN, Bhaskar
发明人： CHESNUT, Jonathan , BENNETT, Robert , BURRIER, Robert , LIEU, Pauline , RAO, Mahendra , TALIANA, Antje , THYAGARAJAN, Bhaskar
IPC分类号： C12N15/09
CPC分类号： C12N15/1086 , C12N15/1082
摘要： The disclosure relates generally to stem cell biology and more specifically to genetic manipulation of stem cells. Methods and compositions using recombinational cloning techniques are disclosed which allow the construction and insertion of complex genetic constructs into embryonic and adult stem cells and progenitor cells. The methods disclosed will allow the harvesting of adult stem cells pre-engineered with integration sites to facilitate early passage genetic modification.
摘要翻译：本公开一般涉及干细胞生物学，更具体地涉及干细胞的遗传操作。公开了使用重组克隆技术的方法和组合物，其允许将复杂遗传构建体构建和插入胚胎和成体干细胞和祖细胞中。所公开的方法将允许收获用整合位点预先设计的成体干细胞，以促进早期遗传修饰。

9. 发明申请

WO2020132607A9 ASYMMETRIC RHODAMINE DYE AND USE THEREOF IN BIOLOGICAL ASSAYS 审中-公开
公开(公告)号：WO2020132607A9
公开(公告)日：2020-06-25
申请号：PCT/US2019/068111
申请日：2019-12-20
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： EVANS, Brian , BENSON, Scott
IPC分类号： C09B11/24 , C07H21/00 , C12N15/65 , G01N33/58
摘要： The present disclosure relates to N-protected NH-rhodamine dyes and their use in nucleic acid detection. In particular, the disclosure relates to methods of making N-protected NH-rhodamine dyes, and methods of use of N-protected NH-rhodamine dyes (e.g., human identification). Certain dyes provided herein have unique spectral properties that complement those in existing dye sets and can be used to expand the number of reporter dyes that can be included for HID applications and other biological assays. Those fluorescent compounds are useful to label synthetic oligonucleotides. Formula (I).

10. 发明申请

WO2018237024A1 MESYLATE BASED MASTER MIX 审中-公开
公开(公告)号：WO2018237024A1
公开(公告)日：2018-12-27
申请号：PCT/US2018/038531
申请日：2018-06-20
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： ZHONG, Chang , MULERO, Julio , MENCHEN, Steven
IPC分类号： C12Q1/686 , C12Q1/6848
摘要： Disclosed herein are mesylate containing compositions, methods of use of such compositions and kits of components encompassing such compositions for improving the amplification of nucleic acids, especially in the presence of enzymatic inhibitors. These find particular use in forensic and environmental analyses. The instant disclosure provides new compositions, methods and kits of components for overcoming PCR inhibition. In some embodiments disclosed herein is a PCR master mix, the PCR master mix encompassing a mesylate.

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