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1. 发明申请

WO2019178042A1 INSECTICIDAL PROTEINS FROM PLANTS AND METHODS FOR THEIR USE 审中-公开
公开(公告)号：WO2019178042A1
公开(公告)日：2019-09-19
申请号：PCT/US2019/021775
申请日：2019-03-12
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , HEXIMA LIMITED
发明人： LIU, Lu , LUM, Amy , ONG, Azalea S. , SCHEPERS, Eric , UDRANSZKY, Ingrid , ZHONG, Xiaohong
IPC分类号： A01N65/00 , C07K14/415 , C12N15/09 , C12N15/52 , C12N15/62 , C12N15/82
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

2. 发明申请

WO2019125651A1 INSECTICIDAL POLYPEPTIDES AND USES THEREOF 审中-公开
公开(公告)号：WO2019125651A1
公开(公告)日：2019-06-27
申请号：PCT/US2018/060878
申请日：2018-11-14
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： ABAD, Andre R. , DONG, Hua , LO, Sue B. , POLAND, Brad , SHI, Xiaomei , WANG, Jimei , WOLFE, Thomas Chad , ZHOU, Lan
IPC分类号： C07K14/32 , C07K14/325 , C12N15/31 , C12N15/32
CPC分类号： C07K14/325 , C12N15/8286
摘要： The disclosure provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidopteran and Coleopteran. Particular embodiments of the disclosure provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

3. 发明申请

WO2013149018A1 MAIZE EVENT DP-004114-3 AND METHODS FOR DETECTION THEREOF 审中-公开
标题翻译：玉米活动DP-004114-3及其检测方法
公开(公告)号：WO2013149018A1
公开(公告)日：2013-10-03
申请号：PCT/US2013/034374
申请日：2013-03-28
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY
发明人： ALARCON, Clara M. , HARMON, Matthew C. , PASCUAL, Maria Alejandra , REGISTER, James C., III , SCELONGE, Christopher J. , YOUNG, Joshua K. , ZHONG, Cathy Xiaoyan
IPC分类号： C12N15/82 , C12Q1/68
CPC分类号： C12Q1/6895 , A01H5/10 , C07K14/325 , C12N15/8209 , C12N15/8286 , C12Q1/686 , C12Q2535/125 , Y02A40/162
摘要： The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-004114-3 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
摘要翻译：本发明提供了涉及转基因抗虫玉米植物的DNA组合物。还提供了基于插入到玉米基因组中的重组构建体的DNA序列和插入位点侧翼的DNA序列来检测玉米DP-004114-3事件的存在的测定法。提供了用于进行测定的试剂盒和条件。

4. 发明申请

WO2017192560A1 INSECTICIDAL PROTEINS AND METHODS FOR THEIR USE 审中-公开
公开(公告)号：WO2017192560A1
公开(公告)日：2017-11-09
申请号：PCT/US2017/030602
申请日：2017-05-02
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DU PONT DE NEMOURS AND COMPANY
发明人： BARRY, Jennifer Kara , BARTHOLOMAY, Christian , D'LIMA, Louisa , ENGLISH, James J. , HAYES, Kevin Robert , LIU, Lu , LUM, Amy , POLAND, Brad , SCHEPERS, Eric Jude , XIE, Weiping , YALPANI, Nasser , ZHU, Genhai
IPC分类号： A01P7/04 , C12N15/82
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

5. 发明申请

WO2017180715A2 INSECTICIDAL POLYPEPTIDES HAVING IMPROVED ACTIVITY SPECTRUM AND USES THEREOF 审中-公开
标题翻译：具有改进的活性谱的杀虫多肽及其用途
公开(公告)号：WO2017180715A2
公开(公告)日：2017-10-19
申请号：PCT/US2017/027160
申请日：2017-04-12
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： HORN, Caroline , LAU, Sabina , IZUMI WILCOXON, Michi , YAMAMOTO, Takashi , ZHENG, Yi
IPC分类号： A01H5/10 , C12N15/82
摘要： The disclosure provides nucleic acids, and variants and fragments thereof, derived from strains of Bacillus thuringiensis encoding variant polypeptides having increased pesticidal activity against insect pests, including Lepidoptera and Coleopteran. Particular embodiments of the disclosure provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
摘要翻译：本公开内容提供了源自编码变体多肽的苏云金芽孢杆菌菌株的核酸及其变体和片段，所述变体多肽对昆虫害虫（包括鳞翅目和鞘翅目昆虫）具有增强的杀虫活性。本公开的具体实施方案提供了编码杀虫蛋白的分离的核酸，杀虫组合物，DNA构建体和包含所述实施方案的核酸的转化的微生物和植物。这些组合物可用于防治害虫，特别是植物害虫的方法。

6. 发明申请

WO2016186986A1 INSECTICIDAL PROTEINS AND METHODS FOR THEIR USE 审中-公开
公开(公告)号：WO2016186986A1
公开(公告)日：2016-11-24
申请号：PCT/US2016/032273
申请日：2016-05-13
申请人： PIONEER HI BRED INTERNATIONAL INC
发明人： KOZY, Heather , ORAL, Jarred , SCHELLENBERGER, Ute , WEI, Jun-Zhi , XIE, Weiping , ZHONG, Xiaohong , ZHU, Genhai
IPC分类号： C12N15/82 , C07K14/195
CPC分类号： C12N15/8242 , C07K14/21 , C07K2319/21
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

7. 发明申请

WO2016144686A1 STRUCTURE BASED METHODS FOR MODIFICATION OF PIP-72 POLYPEPTIDES AND PIP-72 POLYPEPTIDES DERIVED THEREFROM 审中-公开
标题翻译：基于结构的PIP-72多肽和PIP-72多肽衍生物的修饰方法
公开(公告)号：WO2016144686A1
公开(公告)日：2016-09-15
申请号：PCT/US2016/020634
申请日：2016-03-03
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： LIU, Lu , ORAL, Jarred Kenneth , POLAND, Brad , SCHELLENBERGER, Ute
IPC分类号： C12N15/82 , C07K14/415 , C07K14/21 , A01N65/00
CPC分类号： C07K14/21 , A01N63/02 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity. Structure based methods for engineering insecticidal proteins of the disclosure to have modified physical properties are provided.
摘要翻译：提供了控制害虫的组成和方法。该方法涉及用编码杀虫蛋白的核酸序列转化生物体。特别地，核酸序列可用于制备具有杀虫活性的植物和微生物。因此，提供了转化的细菌，植物，植物细胞，植物组织和种子。组合物是杀虫的核酸和细菌物种的蛋白质。这些序列用于构建表达载体，用于随后转化为感兴趣的生物体，包括植物，作为分离其他同源（或部分同源的）基因的探针。杀虫蛋白用于控制，抑制生长或杀死鳞翅目，鞘翅目，双翅目，真菌，半翅目和线虫害虫种群并生产具有杀虫活性的组合物。提供了具有改进的物理性质的本发明的用于工程化杀虫蛋白的基于结构的方法。

8. 发明申请

WO2015023846A2 INSECTICIDAL PROTEINS AND METHODS FOR THEIR USE 审中-公开
标题翻译：杀虫蛋白及其使用方法
公开(公告)号：WO2015023846A2
公开(公告)日：2015-02-19
申请号：PCT/US2014/051063
申请日：2014-08-14
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY
发明人： LIU, Lu , O'REAR, Jessica , PARK, Young Jun , ROSEN, Barbara , SCHELLENBERGER, Ute , SCHEPERS, Eric , WEI, Zun-Zhi , XIE, Weiping , YALPANI, Nasser , ZHONG, Xiaohong , ZHU, Genhai
IPC分类号： C07K14/415 , A01H5/00
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/21 , C12R1/40 , Y02A40/162
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
摘要翻译：提供了用于防治害虫的组合物和方法。该方法涉及用编码杀虫蛋白质的核酸序列转化生物体。特别地，核酸序列可用于制备具有杀虫活性的植物和微生物。因此，提供了转化的细菌，植物，植物细胞，植物组织和种子。组合物是杀虫核酸和细菌物种的蛋白质。该序列可用于构建表达载体，用于随后转化成感兴趣的生物体，包括植物，作为分离其他同源（或部分同源）基因的探针。杀虫蛋白质可用于控制，抑制鳞翅目，鞘翅目，双翅目，真菌，半翅目和线虫害虫种群的生长或杀死以及用于生产具有杀虫活性的组合物。

9. 发明申请

WO2020055885A1 INSECTICIDAL PROTEINS AND METHODS FOR THEIR USE 审中-公开
公开(公告)号：WO2020055885A1
公开(公告)日：2020-03-19
申请号：PCT/US2019/050434
申请日：2019-09-10
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： ALTIER, Daniel J. , BARRY, Jennifer Kara , BARTHOLOMAY, Christian , DONG, Hua , GERBER, Ryan Michael , GILLIAM, Jacob , GRUVER, Steven D. , LIU, Lu , ORAL, Jarred Kenneth , O'REAR, Jessica , SCHELLENBERGER, Ute , SCHEPERS, Eric , THORPE, Daniel James , WOLFE, Thomas Chad , XIE, Weiping , YALPANI, Nasser , YOU, You , ZHU, Genhai
IPC分类号： C07K14/195 , C07K14/21 , C12N15/82
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

10. 发明申请

WO2018005411A1 INSECTICIDAL PROTEINS FROM PLANTS AND METHODS FOR THEIR USE 审中-公开
标题翻译：植物杀虫蛋白及其使用方法
公开(公告)号：WO2018005411A1
公开(公告)日：2018-01-04
申请号：PCT/US2017/039376
申请日：2017-06-27
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： BARRY, Jennifer , FINKE, Catherine , GERBER, Ryan , LUM, Amy , MATHIS, John , ONG, Azalea , PETERSON-BURCH, Brooke , WOLFE, Thomas C. , XIE, Weiping , YALPANI, Nasser , ZHONG, Xiaohong
IPC分类号： A01H1/00 , A01H5/00 , A01N63/00 , C12N15/09 , C12N15/31
摘要： Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
摘要翻译：提供了用于防治害虫的组合物和方法。该方法涉及用编码杀虫蛋白质的核酸序列转化生物体。特别地，核酸序列可用于制备具有杀虫活性的植物和微生物。因此，提供了转化的细菌，植物，植物细胞，植物组织和种子。组合物是杀虫核酸和细菌物种的蛋白质。该序列可用于构建表达载体，用于随后转化成感兴趣的生物体，包括植物，作为分离其他同源（或部分同源）基因的探针。杀虫蛋白质可用于控制，抑制鳞翅目，鞘翅目，双翅目，真菌，半翅目和线虫害虫种群的生长或杀死以及用于生产具有杀虫活性的组合物。

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