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1. 发明申请

WO2004108741A1 PROCESS FOR THE CONCENTRATION AND/OR ISOLATION OF NUCLEIC ACID OR NUCLEIC ACID-CONTAINING SPECIES 审中-公开
标题翻译：浓缩和/或分离含有核酸或含核酸的物种的方法
公开(公告)号：WO2004108741A1
公开(公告)日：2004-12-16
申请号：PCT/EP2004/005998
申请日：2004-06-03
申请人： QIAGEN AS , QIAGEN GMBH , DEGGERDAL, Arne , REITAN, Evy, H. , SKAGESTAD, Vidar
发明人： DEGGERDAL, Arne , REITAN, Evy, H. , SKAGESTAD, Vidar
IPC分类号： C07H1/08
CPC分类号： C12N15/1003
摘要： The present invention relates to a process for the concentration and/or isolation of nucleic acids or nucleic acid-containing species from a nucleic acid-containing solution, and a kit therefor. In one embodiment, the invention relates to the concentration and/or isolation of DNA and RNA from nucleic acid-containing solutions.
摘要翻译：本发明涉及从含核酸的溶液中浓缩和/或分离核酸或含核酸物质的方法及其用途。在一个实施方案中，本发明涉及来自含核酸溶液的DNA和RNA的浓度和/或分离。

2. 发明申请

WO2003095646A1 ISOLATING NUCLEIC ACID 审中-公开
标题翻译：分离核酸
公开(公告)号：WO2003095646A1
公开(公告)日：2003-11-20
申请号：PCT/IB2003/001822
申请日：2003-05-09
申请人： QIAGEN AS , DEGGERDAL, Arne , SKAGESTAD, Vidar
发明人： DEGGERDAL, Arne , SKAGESTAD, Vidar
IPC分类号： C12N15/10
CPC分类号： C12N15/1006
摘要： A process. for isolating nucleic acid from a nucleic acid containing sample, which comprises: (a) providing a chaotrope; (b) providing a nucleic acid binding solid phase capable of binding nucleic acid in the presence of the chaotrope; (c) contacting the sample with the nucleic acid binding solid phase in the presence of a liquid phase comprising the chaotrope; and (e) optionally separating the solid phase with the nucleic acid bound thereto from the liquid phase, wherein the solid phase bears acid groups on its surface.
摘要翻译：一个过程。用于从含有核酸的样品中分离核酸，其包括：（a）提供离液剂; （b）提供能够在离液剂存在下结合核酸的核酸结合固相; （c）在包含离液剂的液相存在下使样品与核酸结合固相接触; 和（e）任选地将所述固相与与所述液相结合的核酸分离，其中所述固相在其表面上具有酸性基团。

3. 发明申请

WO2003091452A1 ISOLATING NUCLEIC ACID 审中-公开
标题翻译：分离核酸
公开(公告)号：WO2003091452A1
公开(公告)日：2003-11-06
申请号：PCT/IB2003/001553
申请日：2003-04-22
申请人： QIAGEN AS , DEGGERDAL, Arne , SKAGESTAD, Vidar
发明人： DEGGERDAL, Arne , SKAGESTAD, Vidar
IPC分类号： C12Q1/68
CPC分类号： C12N15/1013 , C12Q1/6806 , C12Q2563/143 , C12Q2527/125
摘要： A process for isolating nucleic acid from a nucleic acid- containing biological sample, which comprises: (a) providing magnetic particles capable of binding nucleic acid; (b) contacting the sample with the magnetic particles so as to bind the nucleic acid thereto; (c) forming an aggregate of the magnetic particles by the application of a magnetic field, which aggregate comprises magnetic particles with the nucleic acid bound thereto and contaminants from the biological sample; (d) contacting the aggregate with a washing solution to disperse the aggregate and release the contaminants therefrom; and (e) separating therefrom the magnetic particles with the nucleic acid bound thereto; wherein the aggregate is formed in the presence of an amount of a water-miscible lower alcohol sufficient to facilitate dispersion of the aggregate and release of the contaminants therefrom when washed in step (d), with the proviso that, when the magnetic particles are silica-based magnetic glass particles provided with the water-miscible lower alcohol, the water-miscible lower alcohol is part of an aqueous mixture which further comprises a salt.
摘要翻译：一种用于从含核酸的生物样品中分离核酸的方法，其包括：（a）提供能够结合核酸的磁性颗粒; （b）使样品与磁性颗粒接触以使核酸与其结合; （c）通过施加磁场形成磁性颗粒的聚集体，该聚集体包含与其结合的核酸的磁性颗粒和来自生物样品的污染物; （d）使聚集体与洗涤溶液接触以分散聚集体并从其中释放污染物; 和（e）与其结合的核酸与其分离; 其中所述聚集体在一定量的水混溶性低级醇存在下形成，所述量可以在步骤（d）中洗涤时足以促进聚集体的分散和其中的污染物的释放，条件是当磁性颗粒是二氧化硅提供有水混溶性低级醇的基于水的磁性玻璃颗粒，水混溶性低级醇是进一步包含盐的含水混合物的一部分。

4. 发明申请

WO2003084976A1 PROCESS FOR ISOLATING NUCLEIC ACID WITH CHAOTROPE AGENTS AND AMMONIUM COMPOUNDS 审中-公开
标题翻译：用水解剂和氨基化合物分离核酸的方法
公开(公告)号：WO2003084976A1
公开(公告)日：2003-10-16
申请号：PCT/IB2003/001202
申请日：2003-04-02
申请人： QIAGEN AS , REITAN, Evy , DEGGERDAL, Arne , SKAGESTAD, Vidar
发明人： REITAN, Evy , DEGGERDAL, Arne , SKAGESTAD, Vidar
IPC分类号： C07H21/00
CPC分类号： C12N15/1006
摘要： A process for isolating nucleic acid from a nucleic acid-containing sample, which comprises: (a) providing a chaotrope; (b) providing a nucleic acid binding solid phase capable of binding nucleic acid in the presence of the chaotrope; (c) providing a source of NH4+ or NH3; (d) contacting the sample with the nucleic acid binding solid phase in the presence of a liquid phase comprising the chaotrope and the NH4+ or NH3; and (e) optionally separating the solid phase with the nucleic acid bound thereto from the liquid phase.
摘要翻译：一种从含核酸样品中分离核酸的方法，包括：（a）提供离液剂; （b）提供能够在离液剂存在下结合核酸的核酸结合固相; （c）提供NH4 +或NH3的来源; （d）在包含离液剂和NH 4 +或NH 3的液相存在下使样品与核酸结合固相接触; 和（e）任选地将所述固相与与液相结合的核酸分离。

5. 发明申请

WO2007023057A1 VERFAHREN ZUR GEWINNUNG VON NUKLEINSÄUREN AUS BLUT 审中-公开
标题翻译：一种获得NUCLEIC从血液
公开(公告)号：WO2007023057A1
公开(公告)日：2007-03-01
申请号：PCT/EP2006/064652
申请日：2006-07-25
申请人： QIAGEN GMBH , QIAGEN AS , ERBACHER, Christoph , HIMMELREICH, Ralf , PEIST, Ralf , EVANS HAALAND, Ingerlise , HARDERSEN, Hege
发明人： ERBACHER, Christoph , HIMMELREICH, Ralf , PEIST, Ralf , EVANS HAALAND, Ingerlise , HARDERSEN, Hege
IPC分类号： C12N15/10
CPC分类号： C12Q1/6813 , C08F8/30 , C08F8/40 , C12N5/0081 , C12Q1/6806 , C12Q1/6844 , C08F222/06
摘要： Die vorliegende Erfindung betrifft ein verbessertes Verfahren zur Isolierung von Nukleinsäuren insbesondere von genomischer Desoxyribonukleinsäure (DNA) aus Blut.
摘要翻译：本发明涉及用于核酸的分离的改进的方法，在从血液中特定基因组脱氧核糖核酸（DNA）。

6. 发明申请

WO2005093101A1 NUCLEIC ACID SEQUENCING 审中-公开
标题翻译：核酸序列
公开(公告)号：WO2005093101A1
公开(公告)日：2005-10-06
申请号：PCT/IB2005/000771
申请日：2005-03-24
申请人： QIAGEN AS , LARSEN, Frank
发明人： LARSEN, Frank
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6858 , C12Q1/6813 , C12Q1/6869 , C12Q2565/301 , C12Q2535/125 , C12Q2525/186
摘要： A method for determining a target nucleic acid sequence, wherein the target nucleic acid sequence is comprised in a preparation comprising a non-target nucleic acid sequence, the target nucleic acid sequence and the non-target nucleic acid sequence each having a first region of common sequence upstream of a first region of dissimilar sequence upstream of a second region of dissimilar sequence, the method comprising: (a) contacting the preparation with a blocking oligonucleotide complementary to at least a portion of the first region of dissimilar sequence of the non-target nucleic acid sequence, under conditions to hybridise the blocking oligonucleotide thereto; (b) contacting the preparation with a sequencing primer complementary to at least a portion of the first region of common sequence, under conditions to hybridise the primer to the target nucleic acid sequence; and (c) subjecting the preparation to a sequencing reaction, such that the sequencing reaction proceeds into the second region of dissimilar sequence of the target nucleic acid sequence, thereby determining at least the second region of dissimilar sequence of the target nucleic acid sequence; and wherein the blocking oligonucleotide blocks the sequencing reaction at least from proceeding into the second region of dissimilar sequence of the non-target nucleic acid sequence.
摘要翻译：一种确定靶核酸序列的方法，其中所述靶核酸序列包含在包含非靶核酸序列，所述靶核酸序列和所述非靶核酸序列的制备中，所述靶核酸序列各自具有第一共同区域在不同序列的第二区域上游的不同序列的第一区域的上游的序列，所述方法包括：（a）使所述制剂与所述非靶标的不同序列的第一区域的至少一部分互补的阻断寡核苷酸在将所述封闭寡核苷酸杂交的条件下，（b）在将引物与目标核酸序列杂交的条件下，使制备物与在共有序列的第一区域的至少一部分互补的测序引物接触; 和（c）对所述制剂进行测序反应，使得所述测序反应进行到所述靶核酸序列的不同序列的第二区域，从而至少确定所述靶核酸序列的不同序列的第二区域; 并且其中所述阻断寡核苷酸至少阻止所述测序反应进入所述非靶核酸序列的不同序列的第二区域。

7. 发明申请

WO2005093099A1 NUCLEIC ACID SEQUENCING 审中-公开
标题翻译：核酸序列
公开(公告)号：WO2005093099A1
公开(公告)日：2005-10-06
申请号：PCT/IB2005/000761
申请日：2005-03-24
申请人： QIAGEN AS , LARSEN, Frank
发明人： LARSEN, Frank
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6858 , C12Q1/6827 , C12Q1/6869 , C12Q2535/125 , C12Q2565/301
摘要： A method for determining a target nucleic acid sequence, wherein the target nucleic acid sequence is comprised in a preparation comprising a non-target nucleic acid sequence, the target nucleic acid sequence and the non-target nucleic acid sequence each having a first region of common sequence upstream of a first region of dissimilar sequence upstream of a second region of dissimilar sequence, the method comprising: (a) contacting the preparation with an oligonucleotide primer complementary to at least a portion of the first region of common sequence, under conditions to hybridise the primer thereto; and (b) subjecting the preparation to a sequencing reaction, such that the sequencing reaction proceeds into the second region of dissimilar sequence of the target nucleic acid sequence, thereby determining at least the second region of dissimilar sequence -of the target-nucleic acid- sequence; and wherein the method further comprises a step of blocking the sequencing reaction between the primer and the non-target nucleic acid sequence, such that the sequencing reaction does not proceed into the second region of dissimilar sequence of the nontarget nucleic acid sequence.
摘要翻译：一种确定靶核酸序列的方法，其中所述靶核酸序列包含在包含非靶核酸序列，所述靶核酸序列和所述非靶核酸序列的制备中，所述靶核酸序列各自具有第一共同区域在不同序列的第二区域上游的不同序列的第一区域上游的序列，所述方法包括：（a）使所述制剂与在共有序列的第一区域的至少一部分互补的寡核苷酸引物在杂交条件下接触引物; 和（b）使制备物进行测序反应，使得测序反应进入目标核酸序列的不同序列的第二区域，由此至少确定靶核酸序列的不同序列的第二区域，序列; 并且其中所述方法还包括阻断引物和非靶核酸序列之间的测序反应的步骤，使得测序反应不进入非目标核酸序列的不同序列的第二区域。

8. 发明申请

WO2004108925A1 METHOD FOR SEQUENTIALLY ISOLATING DNA AND RNA FROM THE SAME NUCLEIC ACID-CONTAINING SAMPLE 审中-公开
标题翻译：用于从相同的含有核酸的样品中顺序分离DNA和RNA的方法
公开(公告)号：WO2004108925A1
公开(公告)日：2004-12-16
申请号：PCT/EP2004/005999
申请日：2004-06-03
申请人： QIAGEN AS , QIAGEN GMBH , REITAN, Evy, H. , DEGGERDAL, Arne , SKAGESTAD, Vidar
发明人： REITAN, Evy, H. , DEGGERDAL, Arne , SKAGESTAD, Vidar
IPC分类号： C12N15/10
CPC分类号： C12N15/1003 , C12N15/1006
摘要： The present invention refers to a method for isolating nucleic acids from a nucleic acid-containing sample. More specifically, it relates to a method for sequentially isolating DNA and RNA from the same nucleic acid-containing sample. Furthermore, the present invention refers to kit for sequentially isolating DNA and RNA from the same nucleic acid-containing sample.
摘要翻译：本发明涉及从含核酸样品中分离核酸的方法。更具体地说，本发明涉及从相同的含有核酸的样品中顺序分离DNA和RNA的方法。此外，本发明涉及用于从相同的含有核酸的样品中顺序分离DNA和RNA的试剂盒。

9. 发明申请

US20110257018A1 NUCLEIC ACID SEQUENCING 审中-公开
标题翻译：核酸序列
公开(公告)号：US20110257018A1
公开(公告)日：2011-10-20
申请号：US13101740
申请日：2011-05-05
申请人： FRANK LARSEN
发明人： FRANK LARSEN
IPC分类号： C40B20/00 , C12Q1/68
CPC分类号： C12Q1/6869 , C12Q2565/301 , C12Q2537/163
摘要： A method for determining a target nucleic acid sequence is disclosed, wherein a preparation having a first region of common sequence upstream of a first region of dissimilar sequence upstream of a second region of dissimilar sequence, is contacted with a blocking oligonucleotide complementary to at least a portion of the first region of dissimilar sequence of the non-target nucleic acid sequence, under conditions to hybridise the blocking oligonucleotide thereto and hybridized to the target nucleic acid sequence; and then sequenced, such that the sequencing reaction proceeds into the second region of dissimilar sequence of the target nucleic acid sequence, whereby at least the second region of dissimilar sequence of the target nucleic acid sequence is determined; and wherein the sequencing reaction is blocked at least from proceeding into the second region of dissimilar sequence of the non-target nucleic acid sequence.
摘要翻译：公开了一种用于确定靶核酸序列的方法，其中将具有在不相似序列的第二区域上游的不同序列的第一区域的上游的共同序列的第一区域的制剂与至少一个在与所述靶核酸序列杂交的条件下，将所述非靶核酸序列的第一区域的不相似序列的部分杂交; 然后进行测序，使得测序反应进入目标核酸序列的不同序列的第二区域，由此确定靶核酸序列的至少第二区域的不同序列; 并且其中所述测序反应至少阻止进入所述非靶核酸序列的不同序列的第二区域。

10. 发明申请

US20090018323A1 METHOD FOR EXTRACTING NUCLEIC ACID FROM BLOOD 有权
标题翻译：从血液中提取核酸的方法
公开(公告)号：US20090018323A1
公开(公告)日：2009-01-15
申请号：US12064321
申请日：2006-07-25
申请人： Christoph Erbacher , Ralf Himmelreich , Ralf Peist , Ingerlise Evans Haaland , Hege Hardersen
发明人： Christoph Erbacher , Ralf Himmelreich , Ralf Peist , Ingerlise Evans Haaland , Hege Hardersen
IPC分类号： C07H21/00 , C12M1/00 , C08H5/00
CPC分类号： C12Q1/6813 , C08F8/30 , C08F8/40 , C12N5/0081 , C12Q1/6806 , C12Q1/6844 , C08F222/06
摘要： The present invention relates to an improved method for isolating nucleic acids, particularly genomic desoxyribonucleic acid (DNA) from blood.
摘要翻译：本发明涉及一种从血液中分离核酸，特别是基因组脱氧核糖核酸（DNA）的改进方法。

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