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1. 发明公开

EP0939830A1 A METHOD OF IMPROVING THE PRODUCTION OF BIOMASS OR A DESIRED PRODUCT FROM A CELL 失效
标题翻译：方法提高所需产物的制备从细胞的
公开(公告)号：EP0939830A1
公开(公告)日：1999-09-08
申请号：EP97938813.0
申请日：1997-09-08
申请人： Jensen, Peter Ruhdal
发明人： JENSEN, Peter, Ruhdal , SNOEP, Jacky, Leendert , WESTERHOFF, Hans, Victor
IPC分类号： C12N15 , C12N1 , C12N9 , C12P1 , C12Q1
CPC分类号： C12N1/16 , C12N1/20 , C12N9/14 , C12P1/00
摘要： The production of biomass or a desired product from a cell can be improved by inducing conversion of ATP to ADP without primary effects on other cellular metabolites or functions which is achieved by expressing an uncoupled ATPase activity in said cell and incubating the cell with a suitable substrate to produce said biomass or product. This is conveniently done by expressing in said cell the soluble part (F1) of the membrane bound (F0F1 type) H+-ATPase or a portion of F¿1? exhibiting ATPase activity. The organism from which the F1 ATPase or portions thereof is derived, or in which the F1 ATPase or portions thereof is expressed, may be selected from prokaryotes and eukaryotes. In particular the DNA encoding F1 or a portion thereof may be derived from bacteria and eukaryotic microorganisms such as yeasts, other fungi and cell lines of higher organisms and be selected from the group consisting of the gene encoding the F1 subunit β or a portion thereof and various combinations of said gene or portion with the genes encoding the other F1 subunits or portions thereof. The method can be used i.a. for optimizing the formation of biomass or a desired product by a cell by expressing different levels of uncoupled ATPase activity in the cell, incubating the cell on a suitable substrate, measuring the conversion rate of substrate into biomass or the desired product at each level of ATPase expression, and choosing a level of ATPase expression at which the conversion rate is optimized.

2. 发明公开

EP0934406A2 ARTIFICIAL PROMOTER LIBRARIES FOR SELECTED ORGANISMS AND PROMOTERS DERIVED FROM SUCH LIBRARIES 失效
标题翻译：人工启动子库中选择的生物和文章ENTSTAMMENDE发起人
公开(公告)号：EP0934406A2
公开(公告)日：1999-08-11
申请号：EP97936613.0
申请日：1997-08-25
申请人： Jensen, Peter Ruhdal
发明人： JENSEN, Peter Ruhdal , HAMMER, Karin
IPC分类号： C12N15
CPC分类号： C12N15/63 , C12N15/1051
摘要： An artificial promoter library for a selected organism or group of organisms is constructed as a mixture of double stranded DNA fragments, the sense strands of which comprise at least two consensus sequences of efficient promoters from said organism or group of organisms, or parts thereof comprising at least half of each, and surrounding or intermediate nucleotide sequences (spacers) of variable length in which at least 7 nucleotides are selected randomly among the nucleobases A, T, C and G. The sense strands of the double stranded DNA fragments may also include a regulatory DNA sequence imparting a specific regulatory feature, such as activation by a change in the growth conditions, to the promoters of the library. Further, they may have a sequence comprising one or more recognition sites for restriction endonucleases added to one of or both their ends. The selected organism or group or organisms may be selected from prokaryotes and from eukaryotes; and in prokaryotes the consensus sequences to be retained most often will comprise the -35 signal (-35 to -30): TTGACA and the -10 signal (-12 to -7): TATAAT or parts of both comprising at least 3 conserved nucleotides of each, while in eukaryotes said consensus sequences should comprise a TATA box and at least one upstream activation sequence (UAS). Such artificial promoter libraries can be used i.a. for optimizing the expression of specific genes in various selected organisms.

3. 发明授权

EP0934406B1 ARTIFICIAL PROMOTER LIBRARIES FOR SELECTED ORGANISMS AND PROMOTERS DERIVED FROM SUCH LIBRARIES 失效
标题翻译：人工启动子库中选择的生物和文章ENTSTAMMENDE发起人
公开(公告)号：EP0934406B1
公开(公告)日：2008-11-19
申请号：EP97936613.5
申请日：1997-08-25
申请人： Jensen, Peter Ruhdal
发明人： JENSEN, Peter Ruhdal , HAMMER, Karin
IPC分类号： C12N15/11 , C12N15/63
CPC分类号： C12N15/63 , C12N15/1051

4. 发明公开

EP2034021A1 Artificial promoter libraries for selected organisms and promoters derived from such libraries 失效
标题翻译： Künstliche促进剂bibliothekenfürausgewählteorganismen sowie daraus entstammende promotoren
公开(公告)号：EP2034021A1
公开(公告)日：2009-03-11
申请号：EP08169178.4
申请日：1997-08-25
申请人： Jensen, Peter Ruhdal
发明人： Jensen, Peter Ruhdal , Hammer, Karin
IPC分类号： C12N15/10
CPC分类号： C12N15/63 , C12N15/1051
摘要： An artificial promoter library for a selected organism or group of organisms is constructed as a mixture of double stranded DNA fragments, the sense strands of which comprise at least two consensus sequences of efficient promoters from said organism or group of organism, or parts thereof comprising at least half of each, and surrounding or intermediate nucleotide sequences (spacers) of variable length in which at least 7 nucleotides are selected randomly among the nucleobases A, T, C and G. The sense strands of the double stranded DNA fragments may also include a regulatory DNA sequence imparting a specific regulatory feature, such as activation by a change in the growth conditions, to the promoters of the library. Further, they may have a sequence comprising one or more recognition sites for restriction endonucleases added to one of or both their ends. The selected organism or group or organisms may be selected from prokaryotes and from eukaryotes; and in prokaryotes the consensus sequences to be retained most often will comprise the -35 signal (-35 to -30): TTGACA and the -10 signal (-12 to -7): TATAAT or parts of both comprising at least 3 conserved nucleotides of each, while in eukaryotes said consensus sequences should comprise a TATA box and at least one upstream activation sequence (UAS). Such artificial promoter libraries can be used i.e. for optimizing the expression of specific genes in various selected organism.
摘要翻译：构建所选生物体或生物体组的人造启动子文库，其为双链DNA片段的混合物，其有义链包含来自所述生物体或生物体组的至少两个有效启动子的共有序列或其部分，其包含每个的至少一半，以及在核碱基A，T，C和G中随机选择至少7个核苷酸的可变长度的周围或中间核苷酸序列（间隔区）。双链DNA片段的有义链还可以包括调节性DNA序列赋予特异性调节特征，例如通过生长条件变化的激活，向文库的启动子。此外，它们可以具有包含一个或多个加入其末端中的一个或两个的限制性内切核酸酶的识别位点的序列。所选生物体或组或生物体可以选自原核生物和真核生物; 并且在原核生物中，最常保留的共有序列将包含-35信号（-35至-30）：TTGACA和-10信号（-12至-7）：TATAAT或两者的部分包含至少3个保守核苷酸而在真核生物中，共有序列应包含TATA盒和至少一个上游激活序列（UAS）。可以使用这样的人工启动子文库，即用于优化各种选择的生物体中的特定基因的表达。

5. 发明授权

EP0939830B1 A METHOD OF IMPROVING THE PRODUCTION OF A DESIRED PRODUCT FROM A CELL 失效
标题翻译：方法提高所需产物的制备从细胞的
公开(公告)号：EP0939830B1
公开(公告)日：2008-12-31
申请号：EP97938813.9
申请日：1997-09-08
申请人： Jensen, Peter Ruhdal
发明人： JENSEN, Peter, Ruhdal , SNOEP, Jacky, Leendert , WESTERHOFF, Hans, Victor
IPC分类号： C12P1/00 , C12N15/67
CPC分类号： C12N1/16 , C12N1/20 , C12N9/14 , C12P1/00
摘要： The production of biomass or a desired product from a cell can be improved by inducing conversion of ATP to ADP without primary effects on other cellular metabolites or functions which is achieved by expressing an uncoupled ATPase activity in said cell and incubating the cell with a suitable substrate to produce said biomass or product. This is conveniently done by expressing in said cell the soluble part (F1) of the membrane bound (F0F1 type) H -ATPase or a portion of F1 exhibiting ATPase activity. The organism from which the F1 ATPase or portions thereof is derived, or in which the F1 ATPase or portions thereof is expressed, may be selected from prokaryotes and eukaryotes. In particular the DNA encoding F1 or a portion thereof may be derived from bacteria and eukaryotic microorganisms such as yeasts, other fungi and cell lines of higher organisms and be selected from the group consisting of the gene encoding the F1 subunit beta or a portion thereof and various combinations of said gene or portion with the genes encoding the other F1 subunits or portions thereof. The method can be used i.a. for optimizing the formation of biomass or a desired product by a cell by expressing different levels of uncoupled ATPase activity in the cell, incubating the cell on a suitable substrate, measuring the conversion rate of substrate into biomass or the desired product at each level of ATPase expression, and choosing a level of ATPase expression at which the conversion rate is optimized.

6. 发明专利

AU4113597A A method of improving the production of biomass or a desired product from a cell 未知
公开(公告)号：AU4113597A
公开(公告)日：1998-03-26
申请号：AU4113597
申请日：1997-09-08
申请人： JENSEN PETER RUHDAL
发明人： JENSEN PETER RUHDAL , SNOEP JACKY LEENDERT , WESTERHOFF HANS VICTOR
IPC分类号： C12N15/09 , C12N1/16 , C12N1/20 , C12N1/21 , C12N9/14 , C12P1/00 , C12Q1/68 , C12N15/67
摘要： The production of biomass or a desired product from a cell can be improved by inducing conversion of ATP to ADP without primary effects on other cellular metabolites or functions which is achieved by expressing an uncoupled ATPase activity in the cell and incubating the cell with a suitable substrate to produce the biomass or product. This is conveniently done by expressing in the cell the soluble part (F 1 ) of the membrane bound (F 0 F 1 type) H + ATPase or a portion of F 1 exhibiting ATPase activity. The organism from which the F 1 ATPase or portions thereof is derived, or in which the F 1 ATPase or portions thereof is expressed, may be selected from prokaryotes and eukaryotes. In particular the DNA encoding F 1 or a portion thereof may be derived from bacteria and eukaryotic microorganisms such as yeasts, other fungi and cell lines of higher organisms and be selected from the group consisting of the gene encoding the F 1 subunit beta or a portion thereof and various combinations of the gene or portion with the genes encoding the other F 1 subunits or portions thereof. The method can be used i.a. for optimizing the formation of biomass or a desired product by a cell by expressing different levels of uncoupled ATPase activity in the cell, incubating the cell on a suitable substrate, measuring the conversion rate of substrate into biomass or the desired product at each level of ATPase expression, and choosing level of ATPase expression at which the conversion rate is optimized.

7. 发明专利

AU723380B2 A method of improving the production of biomass or a desired product from a cell 未知
公开(公告)号：AU723380B2
公开(公告)日：2000-08-24
申请号：AU4113597
申请日：1997-09-08
申请人： JENSEN PETER RUHDAL
发明人： JENSEN PETER RUHDAL , SNOEP JACKY LEENDERT , WESTERHOFF HANS VICTOR
IPC分类号： C12N15/09 , C12N1/16 , C12N1/20 , C12N1/21 , C12N9/14 , C12P1/00 , C12Q1/68 , C12N15/67
摘要： The production of biomass or a desired product from a cell can be improved by inducing conversion of ATP to ADP without primary effects on other cellular metabolites or functions which is achieved by expressing an uncoupled ATPase activity in the cell and incubating the cell with a suitable substrate to produce the biomass or product. This is conveniently done by expressing in the cell the soluble part (F 1 ) of the membrane bound (F 0 F 1 type) H + ATPase or a portion of F 1 exhibiting ATPase activity. The organism from which the F 1 ATPase or portions thereof is derived, or in which the F 1 ATPase or portions thereof is expressed, may be selected from prokaryotes and eukaryotes. In particular the DNA encoding F 1 or a portion thereof may be derived from bacteria and eukaryotic microorganisms such as yeasts, other fungi and cell lines of higher organisms and be selected from the group consisting of the gene encoding the F 1 subunit beta or a portion thereof and various combinations of the gene or portion with the genes encoding the other F 1 subunits or portions thereof. The method can be used i.a. for optimizing the formation of biomass or a desired product by a cell by expressing different levels of uncoupled ATPase activity in the cell, incubating the cell on a suitable substrate, measuring the conversion rate of substrate into biomass or the desired product at each level of ATPase expression, and choosing level of ATPase expression at which the conversion rate is optimized.

8. 发明专利

AU3938397A Artificial promoter libraries for selected organisms and promoters derived from such libraries 未知
公开(公告)号：AU3938397A
公开(公告)日：1998-03-06
申请号：AU3938397
申请日：1997-08-25
申请人： JENSEN PETER RUHDAL
发明人： JENSEN PETER RUHDAL , HAMMER KARIN
IPC分类号： C12N15/09 , C12N15/10 , C12N15/63 , C12N15/11
摘要： An artificial promoter library for a selected organism or group of organisms is constructed as a mixture of double stranded DNA fragments, the sense strands of which comprise at least two consensus sequences of efficient promoters from said organism or group of organism, or parts thereof comprising at least half of each, and surrounding or intermediate nucleotide sequences (spacers) of variable length in which at least 7 nucleotides are selected randomly among the nucleobases A, T, C and G. The sense strands of the double stranded DNA fragments may also include a regulatory DNA sequence imparting a specific regulatory feature, such as activation by a change in the growth conditions, to the promoters of the library. Further, they may have a sequence comprising one or more recognition sites for restriction endonucleases added to one of or both their ends. The selected organism or group or organisms may be selected from prokaryotes and from eukaryotes; and in prokaryotes the consensus sequences to be retained most often will comprise the -35 signal (-35 to -30): TTGACA and the -10 signal (-12 to -7): TATAAT or parts of both comprising at least 3 conserved nucleotides of each, while in eukaryotes said consensus sequences should comprise a TATA box and at least one upstream activation sequence (UAS). Such artificial promoter libraries can be used i.e. for optimizing the expression of specific genes in various selected organism.

9. 发明专利

DE69739121D1 未知
公开(公告)号：DE69739121D1
公开(公告)日：2009-01-02
申请号：DE69739121
申请日：1997-08-25
申请人： JENSEN PETER RUHDAL
发明人： JENSEN PETER RUHDAL , HAMMER KARIN
IPC分类号： C12N15/09 , C12N15/11 , C12N15/10 , C12N15/63
摘要： An artificial promoter library for a selected organism or group of organisms is constructed as a mixture of double stranded DNA fragments, the sense strands of which comprise at least two consensus sequences of efficient promoters from said organism or group of organism, or parts thereof comprising at least half of each, and surrounding or intermediate nucleotide sequences (spacers) of variable length in which at least 7 nucleotides are selected randomly among the nucleobases A, T, C and G. The sense strands of the double stranded DNA fragments may also include a regulatory DNA sequence imparting a specific regulatory feature, such as activation by a change in the growth conditions, to the promoters of the library. Further, they may have a sequence comprising one or more recognition sites for restriction endonucleases added to one of or both their ends. The selected organism or group or organisms may be selected from prokaryotes and from eukaryotes; and in prokaryotes the consensus sequences to be retained most often will comprise the -35 signal (-35 to -30): TTGACA and the -10 signal (-12 to -7): TATAAT or parts of both comprising at least 3 conserved nucleotides of each, while in eukaryotes said consensus sequences should comprise a TATA box and at least one upstream activation sequence (UAS). Such artificial promoter libraries can be used i.e. for optimizing the expression of specific genes in various selected organism.

10. 发明专利

DK0939830T3 未知
公开(公告)号：DK0939830T3
公开(公告)日：2009-04-06
申请号：DK97938813
申请日：1997-09-08
申请人： JENSEN PETER RUHDAL
发明人： JENSEN PETER RUHDAL , SNOEP JACKY LEENDERT , WESTERHOFF HANS VICTOR
IPC分类号： C12N15/09 , C12P1/00 , C12N1/16 , C12N1/20 , C12N1/21 , C12N9/14 , C12N15/67 , C12Q1/68
摘要： The production of biomass or a desired product from a cell can be improved by inducing conversion of ATP to ADP without primary effects on other cellular metabolites or functions which is achieved by expressing an uncoupled ATPase activity in the cell and incubating the cell with a suitable substrate to produce the biomass or product. This is conveniently done by expressing in the cell the soluble part (F 1 ) of the membrane bound (F 0 F 1 type) H + ATPase or a portion of F 1 exhibiting ATPase activity. The organism from which the F 1 ATPase or portions thereof is derived, or in which the F 1 ATPase or portions thereof is expressed, may be selected from prokaryotes and eukaryotes. In particular the DNA encoding F 1 or a portion thereof may be derived from bacteria and eukaryotic microorganisms such as yeasts, other fungi and cell lines of higher organisms and be selected from the group consisting of the gene encoding the F 1 subunit beta or a portion thereof and various combinations of the gene or portion with the genes encoding the other F 1 subunits or portions thereof. The method can be used i.a. for optimizing the formation of biomass or a desired product by a cell by expressing different levels of uncoupled ATPase activity in the cell, incubating the cell on a suitable substrate, measuring the conversion rate of substrate into biomass or the desired product at each level of ATPase expression, and choosing level of ATPase expression at which the conversion rate is optimized.

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