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1. 发明申请

WO2016075327A2 PRODUCTION OF PUFAS IN PLANTS 审中-公开
标题翻译：在植物中生产PUFAS
公开(公告)号：WO2016075327A2
公开(公告)日：2016-05-19
申请号：PCT/EP2015076632
申请日：2015-11-13
申请人： BASF PLANT SCIENCE CO GMBH , BIORIGINAL FOOD & SCIENCE CORP
发明人： SENGER TORALF , MARTY LAURENT , KUNZE IRENE , HAERTEL HEIKO A , BREMMER STEVEN , BREAZEALE STEVEN , BAUER JÖRG , VRINTEN PATRICIA , STYMNE STEN , LINDBERG YILMAZ JENNY , MCELVER JOHN , REIN DIETRICH , ANDRE CARL
IPC分类号： C12N15/82
CPC分类号： C12N15/8247 , C12N9/0071 , C12N9/93 , C12Y114/19 , C12Y114/19003 , C12Y602/01003
摘要： The present invention relates to a T-DNA for expression of a target gene in a plant. The present invention further concerns increasing the tocopherol content of a plant relative to a control plant, comprising expressing in a plant at least one polynucleotide encoding a delta-12-desaturase, at least one polynucleotide encoding a delta-6-desaturase, at least one polynucleotide encoding a delta-6-elongase, and at least one polynucleotide encoding a delta-5-desaturase. Further, the present invention relates to an assay method, comprising providing a plant capable of expressing a delta-12 desaturase. The present invention provides also transgenic Brassica events LBFLFK and LBFDAU and progeny thereof, and cells, seeds, plants comprising DNA diagnostic for these events.
摘要翻译：本发明涉及用于在植物中表达靶基因的T-DNA。本发明还涉及增加植物相对于对照植物的生育酚含量，包括在植物中表达至少一种编码Δ-12-去饱和酶的多核苷酸，至少一种编码δ-6-去饱和酶的多核苷酸，至少一种编码δ-6-延伸酶的多核苷酸和至少一种编码δ-5-去饱和酶的多核苷酸。此外，本发明涉及测定方法，其包括提供能够表达Δ-12去饱和酶的植物。本发明还提供了转基因芸苔事件LBFLFK和LBFDAU及其后代，以及包含对这些事件诊断的DNA的细胞，种子，植物。

2. 发明申请

WO2010023202A3 NUCLEIC ACIDS ENCODING DESATURASES AND MODIFIED PLANT OIL 审中-公开
标题翻译：核酸编码和改性植物油
公开(公告)号：WO2010023202A3
公开(公告)日：2010-06-24
申请号：PCT/EP2009060923
申请日：2009-08-25
申请人： BASF PLANT SCIENCE GMBH , BIORIGINAL FOOD & SCIENCE CORP , BAUER JOERG , SENGER TORALF , ZANK THORSTEN , QIU XIAO , WU GUOHAI
发明人： BAUER JOERG , SENGER TORALF , ZANK THORSTEN , QIU XIAO , WU GUOHAI
IPC分类号： C12N15/82 , C12N9/00
CPC分类号： C12N15/8247 , C12N5/14 , C12N9/00 , C12N9/0004
摘要： The present invention relates to nucleic acids derived from Drechslera tritici-repentis, Cylindorcarpon herteronema, Diploida natalensis, Stagonospora nodorum, Microdochium nivalae and Periplaneta americana. The invention also relates to the individual coding sequences and to proteins encoded by these sequences in combination with other sequences as well as to a process for converting oleic acid to linoleic acid to Iinoleic acid and the production of arachidonic acid, eicosapentaenoic acid and/or docosahexaenoic acid in a plant.
摘要翻译：本发明涉及来自Drechslera tritici-repentis，Cylindorcarpon herteronema，Diploida natalensis，Stagonospora nodorum，Microdochium nivalae和Periplaneta americana的核酸。本发明还涉及个体编码序列和由这些序列与其它序列组合编码的蛋白质以及将油酸转化为亚油酸至亚油酸的方法，以及花生四烯酸，二十碳五烯酸和/或二十二碳六烯酸的生产酸在植物中。

3. 发明申请

WO2009077478A3 PROMOTERS FROM BRASSICA NAPUS FOR SEED SPECIFIC GENE EXPRESSION 审中-公开
标题翻译：来自芸苔属植物的用于种子特异性基因表达的启动子
公开(公告)号：WO2009077478A3
公开(公告)日：2009-12-10
申请号：PCT/EP2008067492
申请日：2008-12-15
申请人： BASF PLANT SCIENCE GMBH , BIORIGINAL FOOD & SCIENCE CORP , BAUER JOERG , WETJEN TOM , QIU YIAO , WU GUOHAI
发明人： BAUER JOERG , WETJEN TOM , QIU YIAO , WU GUOHAI
IPC分类号： C12N15/82
CPC分类号： C12N15/8234
摘要： The present invention is concerned with means and methods for allowing tissue specific and, in particular, seed specific expression of genes. The present invention, accordingly, relates to a polynucleotide comprising an expression control sequence which allows seed specific expression of a nucleic acid of interest being operatively linked thereto. Moreover, the present invention contemplates vectors, host cells, non-human transgenic organisms comprising the aforementioned polynucleotide as well as methods and uses of such a polynucleotide.
摘要翻译：本发明涉及用于使组织特异性，特别是基因的种子特异性表达的手段和方法。因此，本发明涉及包含表达控制序列的多核苷酸，所述表达控制序列允许目的核酸的种子特异性表达与其可操作地连接。此外，本发明考虑包含上述多核苷酸的载体，宿主细胞，非人转基因生物以及这种多核苷酸的方法和用途。

4. 发明申请

WO2009077478A2 PROMOTERS FROM BRASSICA NAPUS FOR SEED SPECIFIC GENE EXPRESSION 审中-公开
标题翻译：来自BRASSICA NAPUS的种子特异性基因表达的促进剂
公开(公告)号：WO2009077478A2
公开(公告)日：2009-06-25
申请号：PCT/EP2008/067492
申请日：2008-12-15
申请人： BASF PLANT SCIENCE GMBH , Bioriginal Food & Science Corp. , BAUER, Jörg , WETJEN, Tom , QIU, Yiao , WU, Guohai
发明人： BAUER, Jörg , WETJEN, Tom , QIU, Yiao , WU, Guohai
IPC分类号： C12N15/82
CPC分类号： C12N15/8234
摘要： The present invention is concerned with means and methods for allowing tissue specific and, in particular, seed specific expression of genes. The present invention, accordingly, relates to a polynucleotide comprising an expression control sequence which allows seed specific expression of a nucleic acid of interest being operatively linked thereto. Moreover, the present invention contemplates vectors, host cells, non-human transgenic organisms comprising the aforementioned polynucleotide as well as methods and uses of such a polynucleotide.
摘要翻译：本发明涉及允许组织特异性，特别是基因的种子特异性表达的方法和方法。因此，本发明涉及包含表达控制序列的多核苷酸，所述表达控制序列允许与其有效连接的目的核酸的种子特异性表达。此外，本发明考虑了包含上述多核苷酸的载体，宿主细胞，非人转基因生物以及这种多核苷酸的方法和用途。

5. 发明申请

WO2009016208A2 ELONGASEN UND VERFAHREN ZUR HERSTELLUNG MEHRFACH UNGESÄTTIGTER FETTSÄUREN IN TRANSGENEN ORGANISMEN 审中-公开
标题翻译：延伸酶和过程对于在转基因生物生产多不饱和脂肪酸
公开(公告)号：WO2009016208A2
公开(公告)日：2009-02-05
申请号：PCT/EP2008/060007
申请日：2008-07-30
申请人： BASF PLANT SCIENCE GMBH , BIORIGINAL FOOD & SCIENCE CORP. , BAUER, Jörg , QIU, Xiao , VRINTEN, Patricia
发明人： BAUER, Jörg , QIU, Xiao , VRINTEN, Patricia
IPC分类号： C12N15/82 , C12N9/02
CPC分类号： C12N9/1029 , C12N15/8247
摘要： Die vorliegende Erfindung betrifft Polynukleotide aus Pythium irregulare, Rhizobium oryzae und Euglena gracilis, die Elongasen kodieren und zur rekombinanten Herstellung von mehrfach ungesättigten Fettsäuren eingesetzt werden können. Weiterhin betrifft die Erfindung Vektoren, Wirtszellen und transgene nicht-humane Organismen, die die erfindungsgemäßen Polynukleotide enthalten, sowie die von den Polynukleotiden kodierten Polypeptide. Die Erfindung betrifft zudem Antikörper gegen die erfindungsgemäßen Polypeptide. Schließlich betrifft die Erfindung noch Herstellungsverfahren für die mehrfach ungesättigten Fettsäuren und für Öl-, Lipid-und Fettsäurezusammensetzungen und deren Verwendung als Arzneimittel, Kosmetik, Nahrungsmittel, Futtermittel, vorzugsweise Fischfutter oder Nahrungsergänzungsmittel.
摘要翻译：本发明涉及由畸雌腐霉，米曲霉根瘤菌和眼虫编码延伸酶的多核苷酸，可用于重组生产多不饱和脂肪酸。此外，本发明涉及载体，宿主细胞和包含本发明的转基因非人生物的多核苷酸，以及由所述多核苷酸的多肽编码的蛋白质。本发明还涉及抗体与本发明的多肽。最后，本发明涉及一种制造方法，所述多不饱和脂肪酸和油，脂类和脂肪酸组合物和它们作为药物，化妆品，食品，饲料，优选鱼饲料或食品补充剂。

6. 发明申请

WO2006084352A1 NOVEL OMEGA-3 FATTY ACID DESATURASE FAMILY MEMBERS AND USES THEREOF 审中-公开
标题翻译：新的OMEGA-3脂肪酸去饱和家族成员及其用途
公开(公告)号：WO2006084352A1
公开(公告)日：2006-08-17
申请号：PCT/CA2006/000156
申请日：2006-02-07
申请人： BIORIGINAL FOOD & SCIENCE CORP. , VRINTEN, Patricia , QIU, Xiao
发明人： VRINTEN, Patricia , QIU, Xiao
IPC分类号： C12N15/82 , C12Q1/68 , C12N15/53 , C12N9/02 , C12N5/10 , C12N5/04 , A01H5/10 , A01H5/00
CPC分类号： C12N15/8234 , C12N9/0083 , C12N15/8247
摘要： The present invention relates to the isolation of a second omega-3 fatty acid desaturase ( FAD3 ) gene in flax ( Linum usitatissimum ) and its promoter region, as well as the identification of FAD3 mutant alleles, and the development of markers for wild type and mutated alleles. The FAD3 genes encode the enzymes which control omega-3 fatty acid desaturation and, thus, the levels of linolenic acid (18:3(n-3)). Accordingly, the molecules of the present invention can be utilized, for example, to develop markers for the mutations in flax FAD3 genes and for seed-specific modification of fatty acid and protein compositions in plant seeds.
摘要翻译：本发明涉及在亚麻（Linum usitatissimum）及其启动子区域中分离第二个ω-3脂肪酸去饱和酶（FAD3）基因，以及FAD3突变体等位基因的鉴定，以及野生型和突变等位基因 FAD3基因编码控制ω-3脂肪酸去饱和的酶，从而编码亚麻酸（18：3（n-3））的水平。因此，本发明的分子可以用于例如开发亚麻FAD3基因突变的标记物，以及植物种子中脂肪酸和蛋白质组合物的种子特异性修饰。

7. 发明申请

WO2008022963A2 ISOLATION AND CHARACTERIZATION OF A NOVEL PYTHIUM OMEGA 3 DESATURASE WITH SPECIFICITY TO ALL OMEGA 6 FATTY ACIDS LONGER THAN 18 CARBON CHAINS 审中-公开
标题翻译：一个新的磷酸三钙脱硫剂的分离和表征对所有欧米茄6脂肪酸长18个碳链具有特异性
公开(公告)号：WO2008022963A2
公开(公告)日：2008-02-28
申请号：PCT/EP2007058528
申请日：2007-08-16
申请人： BASF PLANT SCIENCE GMBH , BIORIGINAL FOOD & SCIENCE CORP , BAUER JOERG , WU GUOHAI , QIU XIAO
发明人： BAUER JOERG , WU GUOHAI , QIU XIAO
IPC分类号： C12N9/02 , A01K67/027 , C07K14/37 , C07K16/14 , C07K16/40 , C12N5/10 , C12N15/53 , C12N15/63 , C12N15/81 , C12N15/82 , C12P7/64
CPC分类号： C12P7/6472 , A61K8/64 , A61K8/922 , A61K31/202 , A61K2800/10 , A61Q19/00 , C12N9/0006 , C12N9/0083 , C12N9/0093 , C12N15/52 , C12P7/6427 , C12P7/6436
摘要： The present invention relates to a polynucleotide encoding an omega 3 (?-3) desaturase from Pythium irregulare with specificity to long chain polyunsaturated omega 6 (?- 6) fatty acids as well as a vector containing said polynucleotide, and a host cell containing the vector or the polynucleotide. Moreover, the present invention pertains to a polypeptide encoded by the said polynucleotide, antibodies against the polypeptide as well as a method for the manufacture of the polypeptide. Further, encompassed by the present invention are transgenic non-human organisms. Finally, the present invention relates to methods for the manufacture of compounds and oil- fatty acid- or lipid- containing compositions.
摘要翻译：本发明涉及编码具有对长链多不饱和ω-6（α-6）脂肪酸的特异性的不锈钢的ω3（α-3）去饱和酶的多核苷酸，以及含有所述多核苷酸的载体和含有载体或多核苷酸。此外，本发明涉及由所述多核苷酸编码的多肽，针对多肽的抗体以及制备多肽的方法。此外，本发明包括转基因非人生物体。最后，本发明涉及制备化合物和含油脂肪酸或脂质的组合物的方法。

8. 发明申请

WO0226946A2 FAD4, FAD5, FAD5-2, AND FAD6, NOVEL FATTY ACID DESATURASE FAMILY MEMBERS AND USES THEREOF 审中-公开
标题翻译： FAD4，FAD5，FAD5-2和FAD6，新型脂肪酸脱乙酰酶家族成员及其用途
公开(公告)号：WO0226946A2
公开(公告)日：2002-04-04
申请号：PCT/IB0102346
申请日：2001-09-28
申请人： BIORIGINAL FOOD & SCIENCE CORP
发明人： QIU XIAO , HONG HAIPING
IPC分类号： A23L1/29 , A61K38/00 , A61K38/44 , A61P1/16 , A61P3/02 , A61P3/10 , A61P7/00 , A61P7/02 , A61P9/00 , A61P9/10 , A61P9/12 , A61P11/00 , A61P19/02 , A61P19/04 , A61P19/08 , A61P25/18 , A61P25/24 , A61P25/28 , A61P27/02 , A61P29/00 , A61P35/00 , A61P35/02 , A61P35/04 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/02 , C12N15/09 , C12N15/53 , C12N15/82 , C12P7/64 , C12N9/00
CPC分类号： C12N15/8247 , A23K20/158 , A23L33/12 , A23V2002/00 , A61K31/202 , A61K38/00 , C12N9/0071 , C12N9/0083 , C12P7/6427 , C12P7/6472 , C12Y114/19003 , Y02P20/52
摘要： The invention provides isolated nucleic acid molecules which encode novel fatty acid desaturase family members. The invention also provides recombinant expression vectors containing desaturase nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g., DHA.
摘要翻译：本发明提供编码新型脂肪酸去饱和酶家族成员的分离的核酸分子。本发明还提供了含有去饱和酶核酸分子的重组表达载体，已引入表达载体的宿主细胞，以及大规模生产长链多不饱和脂肪酸（LCPUFA）如DHA的方法。

9. 发明申请

WO2001085968A2 PRODUCTION OF CONJUGATED LINOLEIC AND LINOLENIC ACIDS IN PLANTS 审中-公开
标题翻译：在植物中生产结合的亚硝酸和亚麻酸
公开(公告)号：WO2001085968A2
公开(公告)日：2001-11-15
申请号：PCT/IB2001/001059
申请日：2001-05-09
申请人： BIORIGINAL FOOD & SCIENCE CORP.
发明人： QIU, Xiao
IPC分类号： C12N15/82
CPC分类号： C12N9/0083 , C12N15/8247 , C12P7/6427 , C12P7/6472 , C12Y114/19006
摘要： The present invention relates to methods for large-scale production of conjugated fatty acids, especially conjugated linoleic acids in plants. More specifically, the present invention relates to genes identified from Calendula officinalis coding for a conjugase and its related enzyme, a Δ12 desaturase, and utilization of them for large scale production of conjugated linoleic and linolenic acids in plants. The genes encoding a conjugase can introduce two conjugated double bonds at 8 and 10 positions, and a Δ12 desaturase can introduce a double bond at 12 position of acyl chains. The constructs containing these genes can be transferred to plants with different substrate profiles, which allows for the production of conjugated linoleic acids (18:2, Δ8, Δ10) and linolenic acids (18:3, Δ8, Δ10, Δ12) in plant seeds on a commercial scale.
摘要翻译：本发明涉及用于在植物中大规模生产共轭脂肪酸，特别是共轭亚油酸的方法。更具体地说，本发明涉及从编码结合酶及其相关酶的Calendula officinalis鉴定的基因，DELTA 12去饱和酶，以及它们用于大规模生产植物中共轭亚油酸和亚麻酸的基因。编码结合酶的基因可以在8位和10位引入两个共轭双键，并且DELTA 12去饱和酶可以在酰基链的12位引入双键。含有这些基因的构建体可以转移到具有不同底物轮廓的植物中，这允许生成共轭亚油酸（18：2，DELTA 8，DELTA 10）和亚麻酸（18：3，DELTA 8，DELTA 10，DELTA 12）在商业规模的植物种子中。

10. 发明申请

WO2009016208A3 ELONGASES AND METHODS FOR PRODUCING POLYUNSATURATED FATTY ACIDS IN TRANSGENIC ORGANISMS 审中-公开
标题翻译：延伸酶和过程对于在转基因生物生产多不饱和脂肪酸
公开(公告)号：WO2009016208A3
公开(公告)日：2009-05-07
申请号：PCT/EP2008060007
申请日：2008-07-30
申请人： BASF PLANT SCIENCE GMBH , BIORIGINAL FOOD & SCIENCE CORP , BAUER JOERG , QIU XIAO , VRINTEN PATRICIA
发明人： BAUER JOERG , QIU XIAO , VRINTEN PATRICIA
IPC分类号： C12N15/82 , C12N9/02
CPC分类号： C12N9/1029 , C12N15/8247
摘要： The invention relates to polynucleotides from Pythium irregulare, Rhizobium oryzae and Euglena gracilis which code elongases and which can be used for the recombinant production of polyunsaturated fatty acids. The invention also relates to vectors, host cells and transgenic non-human organisms that contain said inventive polynucleotides, and also the polypeptides coded by said polynucleotides. The invention further relates to anti-bodies against said inventive polypeptides. Finally, the invention relates to production methods for polyunsaturated fatty acids, for oil, lipid and fatty acid compositions and to the use thereof as medicaments, cosmetics, food, animal food in particularly fish food or food supplements.
摘要翻译：本发明涉及由畸雌腐霉，米曲霉根瘤菌和眼虫编码延伸酶的多核苷酸，可用于重组生产多不饱和脂肪酸。此外，本发明涉及载体，宿主细胞和包含本发明的转基因非人生物的多核苷酸，以及由所述多核苷酸的多肽编码的蛋白质。本发明还涉及抗本发明多肽的抗体。最后，本发明涉及一种制造方法，所述多不饱和脂肪酸和油，脂类和脂肪酸组合物和它们作为药物，化妆品，食品，饲料，优选鱼饲料或食品补充剂。

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