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    • 81. 发明申请
    • Methods for producing recombinant polyclonal immunoglobulins
    • 重组多克隆免疫球蛋白的制备方法
    • US20050260710A1
    • 2005-11-24
    • US11092988
    • 2005-03-30
    • Kazuo SuzukiMasahiro FurutaniKenji YamamotoNaohito OhnoKei TakahashiYasuaki ArataniAkiko Togi
    • Kazuo SuzukiMasahiro FurutaniKenji YamamotoNaohito OhnoKei TakahashiYasuaki ArataniAkiko Togi
    • C07K16/00C07K16/44C12N1/21C12N15/74C12P21/06
    • C07K16/00A61K2039/505C07K2317/52C07K2317/622C07K2319/00
    • The purpose of the present invention is to provide methods for producing recombinant polyclonal immunoglobulins using recombinant DNA techniques, which provides constant supply of immunoglobulin preparations with minimum risk of infection. A method of the present invention comprises the following steps: (1) isolating a plurality of types of genes from cDNAs derived from tissues or cells expressing immunoglobulins, said genes encoding a plurality of types of polypeptides respectively, and said polypeptides containing a plurality of types of immunoglobulin variable regions respectively, and preparing mixture of the genes; (2) preparing a plurality of types of recombinant vectors into which a plurality of types of genes are introduced respectively by contacting said mixture of the genes with vectors, and preparing mixture of the recombinant vectors; (3) preparing a plurality of types of transformants into which a plurality of types of recombinant vectors are introduced respectively by contacting said mixture of the recombinant vectors with host cells, and preparing mixture of the transformants; and (4) culturing said mixture of the transformants, and obtaining mixture of polypeptides from the transformants culture, wherein said polypeptides contain a plurality of types of immunoglobulin variable regions respectively.
    • 本发明的目的是提供使用重组DNA技术制备重组多克隆免疫球蛋白的方法,其提供具有最小感染风险的免疫球蛋白制剂的恒定供应。 本发明的方法包括以下步骤:(1)从衍生自表达免疫球蛋白的组织或细胞的cDNA分离多种类型的基因,所述基因分别编码多种类型的多肽,所述多肽含有多种类型 的免疫球蛋白可变区,并制备基因的混合物; (2)通过将所述基因混合物与载体接触,制备多种类型的重组载体,其中分别导入多种类型的基因,并制备重组载体的混合物; (3)通过使重组载体的混合物与宿主细胞接触,制备分别引入多种类型的重组载体的多种类型的转化体,并制备转化体的混合物; (4)培养所述转化体的所述混合物,并从所述转化体培养物获得多肽的混合物,其中所述多肽分别含有多种类型的免疫球蛋白可变区。
    • 86. 发明申请
    • Window regulator
    • 窗口调节器
    • US20050072050A1
    • 2005-04-07
    • US10817815
    • 2004-04-06
    • Kenji Yamamoto
    • Kenji Yamamoto
    • E05F11/38E05F11/48
    • E05F11/488E05F11/385E05F2011/387E05Y2900/55
    • A window regulator to be disposed in a vehicle comprises: a first frame disposed on one side of the longitudinal direction of a door and extending in the directions for a door glass to go up and down; a second frame disposed on the other side of the longitudinal direction of the door and extending in the upward and downward directions of the door glass; an upper frame disposed to span the upper portion of the first frame and the upper portion of the second frame; a wire tensed in an annular shape along the first frame, the upper frame and the second frame and having a return portion for a backward run in the first frame; a first wire fixing member disposed on one side of the longitudinal direction of the door glass and retained by the return portion of the wire to move along the first frame; a second wire fixing member disposed on the other side of the longitudinal direction of the door glass and retained by the wire portion to move along the second frame; and a drum disposed on the inner side of the door panel of the door and wound with a portion of the wire for moving the annularly tensed wire back and forth when it is turned.
    • 设置在车辆中的窗口调节器包括:第一框架,其设置在门的纵向方向的一侧上,并且在门玻璃的上下方向上延伸; 第二框架,其设置在所述门的纵向方向的另一侧并且在所述门玻璃的上下方向上延伸; 设置成跨越所述第一框架的上部和所述第二框架的上部的上框架; 沿着所述第一框架,所述上框架和所述第二框架以环形形状拉紧的线材,并且具有用于在所述第一框架中向后延伸的返回部分; 第一线固定构件,其设置在所述门玻璃的纵向方向的一侧上并且被所述线的返回部分保持以沿着所述第一框架移动; 第二线固定构件,其设置在所述门玻璃的纵向方向的另一侧上,并且被所述线部分保持以沿着所述第二框架移动; 以及鼓,其设置在所述门的门面板的内侧,并且缠绕有所述线的一部分,以在旋转时使所述环形拉紧线前后移动。