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    • 83. 发明申请
    • Biopolymer automatic identifying method
    • 生物聚合物自动识别方法
    • US20060100792A1
    • 2006-05-11
    • US10526464
    • 2003-09-04
    • Tohru NatsumeHiroshi Nakayama
    • Tohru NatsumeHiroshi Nakayama
    • G06F19/00
    • H01J49/0009Y10T436/143333Y10T436/24
    • The invention aims to provide a highly accurate automatic biopolymer determination technique utilizing mass spectrometry whereby calibration prior to measurement or the addition of an internal standard to a sample can be eliminated. The biopolymer automatic identifying method of the invention comprises: retrieving a candidate molecule by matching an observed mass value X obtained by mass spectrometry with a predetermined database; selecting an arbitrary number of candidate molecules with high similarity scores; calibrating the observed mass value X using the candidate molecule as an internal standard; calculating relative error Ec between a calibrated mass value Xc and a theoretical mass value M of the candidate molecule; determining the standard deviation SEc of the relative error; determining a tolerance Tc of database search from the standard deviation SEc; and repeating a database search based on the tolerance Tc.
    • 本发明旨在提供使用质谱法的高精度自动生物聚合物测定技术,由此可以消除在测量之前进行校准或向样品中添加内标物。 本发明的生物聚合物自动识别方法包括:通过将通过质谱法获得的观察质量值X与预定数据库相匹配来检索候选分子; 选择具有高相似性分数的任意数量的候选分子; 使用候选分子作为内标校准观察到的质量值X; 计算校准质量值Xc与候选分子的理论质量值M之间的相对误差Ec; 确定相对误差的标准偏差S E S ; 从标准偏差S 确定数据库搜索的容差Tc; 并基于公差Tc重复数据库搜索。
    • 84. 发明申请
    • Method for collecting information on the primary structure of proteins, hydrophobic microparticulate carrier used in same, and system for automatically obtaining information on the primary structure of proteins
    • 收集蛋白质主要结构信息的方法,使用的疏水性微粒载体和自动获得蛋白质一级结构信息的系统
    • US20060084131A1
    • 2006-04-20
    • US11237682
    • 2005-09-29
    • Hiroshi NakayamaHiromi MiuraMasaya Iwaki
    • Hiroshi NakayamaHiromi MiuraMasaya Iwaki
    • G06F19/00C12Q1/37
    • G01N33/6842C07K1/36G01N33/6818
    • A method for obtaining information on the primary structure of peptides comprising fragmenting the proteins, which enables automation of obtaining information on the primary structure of a peptide sample; a device for use in the method; and an automatic analysis system on the primary structure of the peptides. The method for obtaining information on the primary structure of peptides comprising the steps of: denaturing, reducing, and alkylating a protein; immobilizing the reduced alkylated protein obtained on a hydrophobic microparticulate carrier; chemically or enzymatically fragmenting the immobilized protein obtained; and obtaining information on the primary structure of the peptides fragmented. The automatic analysis system on the primary structure of the peptides comprising an automatic fragmentation element for automatically fragmenting a reduced and alkylated protein, an automatic mass spectrometry element for automatically analyzing the fragmented peptides by mass spectrometry, and a data analysis element for analyzing the data obtained by the automatic mass spectrometry element. The automatic fragmentation of the reduced and alkylated protein is conducted using a hydrophobic microparticulate carrier.
    • 一种用于获得关于肽的一级结构的信息的方法,包括使所述蛋白质分裂,其能够自动获得关于肽样品的一级结构的信息; 用于该方法的装置; 以及肽的一级结构的自动分析系统。 获得关于肽的一级结构的信息的方法,包括以下步骤:使蛋白质变性,还原和烷基化; 固定在疏水性微粒载体上得到的还原烷基化蛋白质; 化学或酶促分离得到的固定化蛋白质; 并获得关于片段化的肽的一级结构的信息。 包括用于自动分解还原和烷基化蛋白质的自动碎裂元件的肽的一级结构的自动分析系统,用于通过质谱自动分析片段化肽的自动质谱元件和用于分析获得的数据的数据分析元件 通过自动质谱元件。 还原和烷基化蛋白质的自动碎裂使用疏水性微粒载体进行。
    • 87. 发明申请
    • Magneto-optical recording medium
    • 磁光记录介质
    • US20050030838A1
    • 2005-02-10
    • US10492094
    • 2002-10-01
    • Yutaka WadaHiroshi NakayamaAtsushi TakeuchiMitsuo Arima
    • Yutaka WadaHiroshi NakayamaAtsushi TakeuchiMitsuo Arima
    • G11B11/105G11B7/007G11B7/24
    • G11B11/10586G11B7/00718G11B7/24076G11B7/24079G11B11/10578G11B11/10584
    • A magneto-optical recording medium for MSR reproduction of a high signal quality wherein both faces of a land and a groove are used as recording tracks is disclosed. A magneto-optical recording medium wherein both of a land portion and a groove portion on a substrate are used as recording and reproduction tracks while laser light having passed through an objective lens is illuminated on the recording and reproduction tracks is configured that a ratio Wg/Wl between a track width (Wg) of the groove portion and a track width (Wl) of the land portion is equal to or higher than 0.65 but equal to or lower than 0.85, and, where the wavelength of the laser light is represented by λ, the numerical aperture of the objective lens by NA and the refractive index of the substrate by n, the groove distance Gp is equal to or greater than 1.0×λ/NA but equal to or smaller than 1.2×λ/NA and besides the depth D of the groove is equal to or greater than λ/11 n but equal to or smaller than λ/8 n.
    • 公开了一种用于MSR再现高信号质量的磁光记录介质,其中使用焊盘和凹槽的两个面作为记录磁道。 一种磁光记录介质,其中基板上的接地部分和凹槽部分都用作记录和再现轨道,同时通过物镜的激光照射在记录和再现轨道上,被配置为比率Wg / 槽部的轨道宽度(Wg)与焊盘部的轨道宽度(W1)之间的宽度W1为0.65以上且0.85以下,激光的波长表示为 lambda,物镜的数值孔径由NA和折射率 衬底指数为n,槽距Gp等于或大于1.0x lambda / NA,但等于或小于1.2x lambda / NA,此外凹槽的深度D等于或大于 lambda / 11 n,但等于或小于 lambda / 8 n。