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    • 85. 发明授权
    • Electrophoresis medium membrane
    • 电泳介质膜
    • US5190629A
    • 1993-03-02
    • US604358
    • 1990-10-26
    • Mitsuru SugiharaMasashi Ogawa
    • Mitsuru SugiharaMasashi Ogawa
    • G01N27/447
    • G01N27/44747G01N27/44704
    • An electrophoresis medium membrane comprises a planar support, a planar cover sheet, and a layer of an electrophoresis gel medium provided between the planar support and the planar cover sheet. The electrophoresis gel medium contains an aqueous polyacrylamide gel, which is prepared by cross-linking polymerization of an acrylamide compound and a cross-linking agent in the presence of water and a compound having at least one carbamoyl group as a denaturing agent (or modifier). The layer of the electrophoresis gel medium has both a predetermined gradual change (gradient) in layer thickness and a predetermined gradual change (gradient) in concentrations of the acrylamide compound and the cross-linking agent.
    • 电泳介质膜包括平面支撑体,平面覆盖片和设置在平面支撑体和平面覆盖片之间的电泳凝胶介质层。 电泳凝胶介质含有水性聚丙烯酰胺凝胶,其通过在水和具有至少一个氨基甲酰基的化合物作为变性剂(或改性剂)的存在下交联丙烯酰胺化合物和交联剂的聚合制备, 。 电泳凝胶介质层在丙烯酰胺化合物和交联剂的浓度上具有预定的层厚度逐渐变化(梯度)和预定的逐渐变化(梯度)。
    • 86. 发明授权
    • Method of quantitatively analyzing analyte contained in whole blood
sample
    • 定量分析全血样品中所含分析物的方法
    • US5130258A
    • 1992-07-14
    • US643831
    • 1991-01-18
    • Yoshihiko MakinoMasashi Ogawa
    • Yoshihiko MakinoMasashi Ogawa
    • G01N21/27G01N21/78G01N33/487G01N33/49G01N33/52
    • G01N33/525G01N21/272G01N33/491G01N21/78Y10S435/805Y10S435/81Y10S436/805Y10S436/808Y10S436/824Y10T436/255
    • A method of quantitatively analyzing an analyte contained in a whole blood sample, wherein a dry multi-layered analysis element is used. The method provides particular merits when the used multi-layered analysis element has no light-shielding layer which is interposed, in the conventional analysis element, between a coloring reagent layer and a blood cell separating layer, so that red coloring matters of blood cells are detected from the support side during the step of measuring the optical density of the reflected light. After the optical density due to coloring matters of blood cells trapped by the blood cell separating layer has reached a constant level or background density, the changing rate in optical density is measured and then the measured changing rate is converted to the corresponding content or activity of the analyte through a colorimetric operation, or the total change in optical density is measured and then the substantially constant background density is subtracted therefrom to know the change in optical density caused by a coloring dye or like material formed in the coloring reagent layer in the presence of the analyte, followed by a similar conversion operation performed on the basis of the principle of colorimetry, to determine the content or activity of the analyte.
    • 一种定量分析全血样品中包含的分析物的方法,其中使用干燥的多层分析元件。 当使用的多层分析元件在传统分析元件中没有遮光层在着色剂层和血细胞分离层之间时,该方法提供了特别的优点,使得血细胞的红色着色物质为 在测量反射光的光密度的步骤期间从支撑侧检测到。 在由血细胞分离层捕获的血细胞的着色物质的光密度达到一定水平或背景浓度后,测定光密度的变化率,然后将测定的变化率转换成相应的含量或活性 通过比色操作的分析物或光密度的总变化被测量,然后从其中减去基本上恒定的背景密度,以了解由存在着色剂层中形成的着色染料或类似材料引起的光密度的变化 的分析物,然后根据比色法原理进行类似的转化操作,以确定分析物的含量或活性。
    • 87. 发明授权
    • Electrophoretic method
    • 电泳法
    • US4900416A
    • 1990-02-13
    • US243975
    • 1988-09-13
    • Yoshihiko MakinoMasashi Ogawa
    • Yoshihiko MakinoMasashi Ogawa
    • G01N27/26G01N27/447
    • G01N27/44704G01N27/44747
    • An electrophoretic method comprises the steps of positioning a sample, which contains nucleic acid fragments having different numbers of bases, in an electrophoresis medium composed of an electrophoresis gel medium containing an aqueous poly(meth)acrylamide gel prepared by cross-linking polymerization of a (meth)acrylamide compound and a cross-linking agent in the presence of water and a compound having at least one carbamoyl group as a denaturing agent (or modifier), and applying pulsed electric fields to the electrophoresis medium in two directions, thereby to move the nucleic acid fragments in the presence of molecular sieve effects and to separate the nucleic acid fragments.
    • 电泳方法包括以下步骤:将含有不同碱基数的核酸片段的样品置于电泳介质中,所述电泳介质由电泳凝胶介质组成,所述电泳凝胶介质含有聚(甲基)丙烯酰胺凝胶水溶液, 甲基)丙烯酰胺化合物和交联剂在水和具有至少一个氨基甲酰基的化合物作为变性剂(或改性剂)的存在下进行,并且在两个方向上向电泳介质施加脉冲电场,从而使 核酸片段在分子筛效应的存在下分离核酸片段。