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    • 71. 发明申请
    • DEVICE AND METHOD FOR 3-DIMENSIONAL ALIGNMENT OF PARTICLES IN MICROFABRICATED FLOW CHANNELS
    • 微量流化通道中颗粒三维对准的装置和方法
    • WO98043066A1
    • 1998-10-01
    • PCT/US1998/004639
    • 1998-03-09
    • G01N15/14G01N37/00
    • G01N15/1404Y10S366/03Y10T436/117497Y10T436/118339
    • The present invention provides a sheath flow module made from a first plate of material having formed therein a laminar fluid flow channel; at least two inlets, each inlet joining the laminar flow channel at a junction, the first inlet junction being wider than the second inlet junction, and an outlet from the flow channel. A second plate, e.g. a transparent cover plate, seals the module and allows for optical measurements. A first inlet allows for introduction of a first fluid into the flow channel. The first fluid is the sheath fluid. A second inlet allows for introduction of a second fluid into the sheath fluid while it is flowing through the flow channel. The second fluid is the center fluid. Because the second inlet junction is narrower than the first inlet junction, the center fluid becomes surrounded on both sides by the sheath fluid. After all fluids have been introduced and sheath flow has been achieved, the depth of the flow channel can be decreased, leading to vertical hydrodynamic focusing. Optionally, the width of the flow channel can be decreased, leading to horizontal hydrodynamic focusing. The decrease in depth and width can be gradual or abrupt. The device of the present invention can function in two modes, the sheath flow mode and the particle injector mode, depending on the relative densities of the sheath fluid, the center fluid, and any particles in either fluid.
    • 本发明提供了一种由第一板材料制成的鞘流模块,其中形成有层流流体通道; 至少两个入口,每个入口在接合处连接层流通道,第一入口接合部比第二入口接合部宽,以及来自流动通道的出口。 第二板,例如 透明的盖板,密封模块并允许进行光学测量。 第一入口允许将第一流体引入流动通道。 第一种液体是鞘液。 第二入口允许在鞘流体流过流动通道时将第二流体引入鞘液中。 第二种液体是中心流体。 因为第二入口接合点比第一入口接合点窄,所以中心流体在两侧被护套液体包围。 在所有流体已经被引入并且鞘流已经实现之后,可以减小流动通道的深度,导致垂直的流体动力学聚焦。 可选地,可以减小流动通道的宽度,导致水平的流体动力学聚焦。 深度和宽度的减小可以是渐进的或突然的。 本发明的装置可以根据鞘液,中心流体和任一液体中的任何颗粒的相对密度,以鞘流模式和颗粒注射模式两种模式起作用。