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71. 发明申请

WO2016056566A1 形質転換体およびその製造方法、ならびに乳酸の製造方法审中-公开
标题翻译：其生产方法及其生产方法，以及生产酸乳酸的方法
公开(公告)号：WO2016056566A1
公开(公告)日：2016-04-14
申请号：PCT/JP2015/078394
申请日：2015-10-06
申请人：旭硝子株式会社
发明人：原　太志 , 鹿島　絢子 , 木村　修一郎
IPC分类号： C12N15/09 , C12N1/19 , C12P7/56 , C12R1/645
CPC分类号： C12P7/56 , C12N9/0006 , C12N9/88 , C12N15/52 , C12R1/645 , C12Y101/01027 , C12Y401/01001
摘要：　アルカリによる中和を必要とせずに高い生産性で乳酸を産生でき、乳酸産生能と増殖能の両方に優れた形質転換体およびその製造方法、ならびに該形質転換体を用いた乳酸の製造方法の提供。　シゾサッカロミセス・ポンベを宿主とし、ヒト由来の乳酸脱水素酵素遺伝子が３～５コピー組み込まれ、かつ前記シゾサッカロミセス・ポンベ宿主のピルビン酸脱炭酸酵素をコードする遺伝子群の一部の遺伝子が欠失または失活していることを特徴とする形質転換体、５００ｍＬ容の坂口フラスコに入った、イーストエクストラクト１％、ペプトン２％、およびグルコース６％からなる液体培地１００ｍＬに初発菌体濃度を０．０４ｇ（ｄｃｗ）／Ｌになるように接種し、温度３２℃、１１０ｒｐｍかつ振り幅７ｃｍの振盪条件下で２０時間培養した培養液の菌体濃度が４．０ｇ（ｄｃｗ）／Ｌ以上であり、前記と同じ条件で２０時間培養し得られた菌体を、１１．１％グルコース水溶液に初発菌体濃度が３６ｇ（乾燥菌体換算）／Ｌになるように接種し、温度３２℃、１１０ｒｐｍかつふり幅７ｃｍの振盪条件下で３時間発酵させた発酵液の乳酸濃度が８０ｇ／Ｌ以上である形質転換体、およびこれら形質転換体を用いた乳酸の製造方法。
摘要翻译：提供：能够以高生产率生产乳酸的转化体，而不需要用碱性试剂中和，并且具有优异的乳酸生产能力和优异的增殖效力; 生产转化体的方法; 以及使用该转化体生产乳酸的方法。一种转化体，其特征在于：其宿主是粟酒裂殖酵母; 将3-5份人源乳酸脱氢酶基因整合到其中; 并且属于粟酒裂殖酵母属主体的编码丙酮酸脱羧酶的基因组的一些基因在其中被删除或失活。一种转化体，其特征在于：当将转化体接种到500ml Sakaguchi烧瓶中的100ml液体培养基中时，所述液体培养基包含1％酵母提取物，2％蛋白胨和6％葡萄糖，初始细胞浓度 0.04g（dcw）/ l，然后在32℃温度下振荡110rpm，振动宽度7cm下培养20小时，液体培养物的细胞浓度为4.0g（dcw）/ l或更大; 当以与上述相同的条件下培养20小时获得的细胞以36g（以干细胞计）/ 1的初始细胞浓度接种到11.1％葡萄糖水溶液中，然后发酵3小时在温度32℃，摇动110rpm，振动宽度7cm的条件下，发酵液的乳酸浓度为80g / l以上。使用任何上述转化体生产乳酸的方法。

72. 发明申请

WO2016012557A1 MICROORGANISM STRAINS FOR THE PRODUCTION OF 2.3- BUTANEDIOL 审中-公开
标题翻译：用于生产2.3-丁醇的微生物菌株
公开(公告)号：WO2016012557A1
公开(公告)日：2016-01-28
申请号：PCT/EP2015/066920
申请日：2015-07-23
申请人： ALDERYS
发明人： BREMOND, Mélanie , JAILLARDON, Karine , LOUIS, Dominique , THOMAS, Dominique
IPC分类号： C12P7/16
CPC分类号： C12P7/18 , C12N9/0006 , C12N9/0036 , C12N9/1022 , C12N9/88 , C12N15/52 , C12Y101/01004 , C12Y106/03 , C12Y202/01006 , C12Y401/01001 , C12Y401/01005
摘要： A recombinant yeast having a rcduced pyruvate decarboxylase activity, in the génome of which has been inserted: - one or more nucleic acids encoding an acetolactate synthase or ALS, - one or more nucleic acids encoding an acetolactate decarboxylase or ALD, - one or more nucleic acids encoding a butancdiol dehydrogenase or BDH, and - one or more copies of a nucleic acids encoding a NADH oxidase or NOXE.
摘要翻译：一种重组酵母，其具有一种或多种编码乙酰乳酸合酶或ALS的核酸， - 编码乙酰乳酸脱羧酶或ALD的一种或多种核酸， - 一种或多种核酸编码丁二醇脱氢酶或BDH的酸，以及编码NADH氧化酶或NOXE的核酸的一个或多个拷贝。

73. 发明申请

WO2014037912A2 METHODS FOR PRODUCTION OF ISOBUTANOL THROUGH NUTRIENT STRESS AND GENETICALLY MODIFIED MICROORGANISMS THEREOF 审中-公开
标题翻译：通过营养应激和遗传修饰的微生物生产异丁醇的方法
公开(公告)号：WO2014037912A2
公开(公告)日：2014-03-13
申请号：PCT/IB2013/058345
申请日：2013-09-06
申请人： CELLWORKS RESEARCH INDIA PVT. LTD
发明人： DATTA, Santanu , RAMANUJAN, Kadambi Sarangapani , ANANDKUMAR, Anand , KATAGIHALLIMATH, Nainesh
IPC分类号： C12P7/16
CPC分类号： C12N9/0006 , C12N1/38 , C12N9/0008 , C12N9/1217 , C12N9/88 , C12N15/52 , C12P7/16 , C12Y101/01001 , C12Y101/01027 , C12Y102/0101 , C12Y207/02001 , C12Y401/01001 , Y02E50/10
摘要： The present disclosure relates to an architecture of energy redistribution that can sustain the increased formation of cofactors like NADH/NADPH and key metabolites like pyruvate that are implicated in the production of isobutanol through biotransformation. Genetically modified microorganisms comprising altered genes are disclosed wherein said alteration optionally along with subjecting the genetically modified microorganism to nutrient stress induces redistribution of energy ultimately resulting in maximum production of isobutanol.
摘要翻译：本公开涉及能量再分布的架构，其能够维持伴随NADH / NADPH等辅因子的增加的形成和涉及通过生物转化产生异丁醇的关键代谢物如丙酮酸。公开了包含改变的基因的遗传修饰的微生物，其中所述改变任选地与经遗传修饰的微生物经受营养应激一起诱导能量的重新分配，最终导致异丁醇的最大产生。

74. 发明申请

WO2011161317A2 GENETICALLY MODIFIED FUNGI AND THEIR USE IN LIPID PRODUCTION 审中-公开
标题翻译：遗传改良真菌及其在脂生产中的应用
公开(公告)号：WO2011161317A2
公开(公告)日：2011-12-29
申请号：PCT/FI2011/050594
申请日：2011-06-21
申请人： TEKNOLOGIAN TUTKIMUSKESKUS VTT , KOIVURANTA, Kari , RUOHONEN, Laura , PENTTILÄ, Merja
发明人： KOIVURANTA, Kari , RUOHONEN, Laura , PENTTILÄ, Merja
IPC分类号： C12N9/10
CPC分类号： C12N15/52 , C12N9/0008 , C12N9/1029 , C12N9/88 , C12N9/93 , C12N15/80 , C12P7/6463 , C12P7/649 , C12Y102/0101 , C12Y203/0102 , C12Y401/01001 , C12Y602/01001 , Y02E50/13 , Y02P20/52
摘要： The invention refers to fungal cells, and especially to oleaginous fungal cells that have been genetically modified to produce enzymes of the pyruvate dehydrogenase bypass route to enhance their lipid production. Especially the cells are modified to over-express genes encoding pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD) and/or acetyl-CoA synthetase (ACS), optionally together with a gene encoding diacylglycerol acyltransferase (DAT), or to express genes encoding PDC together with ALD and/or ACS. Methods of producing lipids, biofuels and lubricants using the modified fungi are also disclosed as well as expression cassettes useful therein.A new enzyme having phosholipid: diacylglycerol acyltransferase (PDAT)activity and a polynucleotide encoding it are also disclosed, which are useful in the lipid production.A recombinant Cryptococcus cell and its construction is described.
摘要翻译：本发明涉及真菌细胞，特别是已被遗传修饰以产生丙酮酸脱氢酶旁路途径酶以增强其脂质生产的油质真菌细胞。特别地，细胞被修饰为过度表达编码丙酮酸脱羧酶（PDC），乙醛脱氢酶（ALD）和/或乙酰辅酶A合成酶（ACS）的基因，任选与编码二酰基甘油酰基转移酶（DAT）的基因一起，或表达编码 PDC与ALD和/或ACS。还公开了使用改性真菌生产脂质，生物燃料和润滑剂的方法以及在其中有用的表达盒。还公开了一种新型的具有磷脂：二酰基甘油酰基转移酶（PDAT）活性的酶和编码它的多核苷酸，其可用于脂质描述了重组隐球藻细胞及其构建。

75. 发明申请

WO2011128907A1 A RECOMBINANT PROCESS FOR THE PRODUCTION OF R-AROMATIC α HYDROXY KETONES 审中-公开
标题翻译：用于生产R-芳族羟基酮的重组方法
公开(公告)号：WO2011128907A1
公开(公告)日：2011-10-20
申请号：PCT/IN2010/000511
申请日：2010-08-02
申请人： INDIAN INSTITUTE OF TECHNOLOGY, BOMBAY , EMBIO LIMITED , NORONHA, Santosh , AGRAWAL, Praveen, Kumar , BHAT, P., Jayadeva
发明人： NORONHA, Santosh , AGRAWAL, Praveen, Kumar , BHAT, P., Jayadeva
IPC分类号： C12P7/26 , C12N9/88
CPC分类号： C12P7/26 , C12N9/88 , C12Y401/01001
摘要： A process for preparation of (R)- aromatic α-hydroxy ketones of formula (I), said process occurring in strain(s) of yeast expressing a recombinant pyruvate decarboxylase, having cysteine residues at positions 221 and 222 of PDC1, an isoenzyme of said pyruvate decarboxylase, substituted with glutamate and alanine respectively such that the said mutation being in the regulatory site of pyruvate decarboxylase, selectively favours carboligation reaction over decarboxylation.
摘要翻译：制备式（I）的（R） - 芳族α-羟基酮的方法，所述方法发生在表达重组丙酮酸脱羧酶的酵母菌株中，PDC1的221和222位具有半胱氨酸残基，同工酶所述丙酮酸脱羧酶分别被谷氨酸和丙氨酸取代，使得所述突变位于丙酮酸脱羧酶的调节位点，选择性地有利于脱羧反应。

76. 发明申请

WO2011041415A1 FERMENTIVE PRODUCTION OF ISOBUTANOL USING HIGHLY EFFECTIVE KETOL-ACID REDUCTOISOMERASE ENZYMES 审中-公开
标题翻译：使用高效的乙醇酸还原酶制剂生产ISOBUTMOL
公开(公告)号：WO2011041415A1
公开(公告)日：2011-04-07
申请号：PCT/US2010/050724
申请日：2010-09-29
申请人： BUTAMAX(TM) ADVANCED BIOFUELS LLC , NAGARAJAN, Vasantha , PAUL, Brian, James , SUH, Wonchul , TOMB, Jean-Francois , YE, Rick, W.
发明人： NAGARAJAN, Vasantha , PAUL, Brian, James , SUH, Wonchul , TOMB, Jean-Francois , YE, Rick, W.
IPC分类号： C12P7/16 , C12P7/42 , C12N9/04 , C12N9/88 , C12N15/52 , C12N1/16 , C12N1/21
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/88 , C12N15/52 , C12P7/42 , C12Y101/01008 , C12Y101/01086 , C12Y401/01001 , C12Y401/01072 , C12Y401/02009 , C12Y402/01009 , Y02E50/10 , Y02P20/52
摘要： Ketol-acid reductoisomerase enzymes have been identified that provide high effectiveness in vivo as a step in an isobutanol biosynthetic pathway in bacteria and in yeast. These KARIs are members of a clade identified through molecular phylogenetic analysis called the SLSL Clade.
摘要翻译：已经鉴定了酮醇酸还原异构酶，其在细菌和酵母中作为异丁醇生物合成途径的步骤在体内提供高效力。这些KARI是通过称为SLSL分子的分子系统发育分析鉴定的进化枝的成员。

77. 发明申请

WO2009137574A3 DEVELOPMENT OF STRAINS OF THE THERMOTOLERANT YEAST HANSENULA POLYMORPHA CAPABLE OF ALCOHOLIC FERMENTATION OF STARCH AND XYLAN BY EXPRESSION OF STARCH AND XYLAN DEGRADING ENZYMES 审中-公开
标题翻译：通过淀粉和XYLAN降解酶的表达可以发现热稳定性YEAST HANSENULA多糖的菌株能够发酵淀粉和XYLAN的醇溶性发酵
公开(公告)号：WO2009137574A3
公开(公告)日：2010-04-15
申请号：PCT/US2009042975
申请日：2009-05-06
申请人： ARCHER DANIELS MIDLAND CO , ABBAS CHARLES , SIBIRNY ANDRIY , VORONOVSKY ANDRIY Y
发明人： ABBAS CHARLES , SIBIRNY ANDRIY , VORONOVSKY ANDRIY Y
IPC分类号： C12N1/19 , C12N15/56 , C12P7/06 , C12R1/01
CPC分类号： C12N9/2428 , C12N9/2414 , C12N9/242 , C12P7/06 , C12Y302/01001 , C12Y302/01003 , C12Y302/01008 , C12Y302/01037 , C12Y401/01001 , Y02E50/17 , Y02P20/52
摘要： Genes SWA2 and GAMl from the yeast, Schwanniomyces occidentalis, encoding a- amylase and glucoamylase, respectively, were cloned and expressed in H. polymorpha. The expression was achieved by integration of the SWA2 and GAMl genes into the chromosome of H. polymorpha under operably linked to a strong constitutive promoter of the H. polymorpha - glyceraldehyde-3 -phosphate dehydrogenase gene (HpGAP. Resulting transformants acquired the ability to grow on a minimal medium containing soluble starch as a sole carbon source and can produce Ethanol at high-temperature fermentation from starch up to 10 g/L. A XYN2 gene encoding endoxylanase was obtained from the fungus Trichoderma resee, and a xlnD gene coding for ß-xylosidase was obtained from the fungus Aspergillus niger. Co-expression of these genes was also achieved by integration into the H. polymorpha chromosome under control of the HpGAP promoter. The resulting transformants were capable of growth on a minimal medium supplemented with birchwood xylan as a sole carbon source. Successful expression of xylanolytic enzymes resulted in a recipient strain capable of fermentation of birchwood xylan to ethanol at 48° C. Further with co expression of the forgoing genes in a H. polymorpha strain that overexpresses a pyruvate decarboxylase gene further improved ethanol production.
摘要翻译：来自酵母的基因SWA2和GAM1分别编码α-淀粉酶和葡糖淀粉酶，分别在多形汉逊酵母中表达。通过与多形汉逊酵母 - 甘油醛-3-磷酸脱氢酶基因（HpGAP）的强组成型启动子可操作地连接，将SWA2和GAM1基因整合到多形汉逊酵母的染色体中来实现该表达。所得到的转化体获得了生长能力在含有可溶性淀粉作为唯一碳源的基本培养基上，可以从淀粉高温发酵至10g / L生产乙醇，从真菌木霉菌获得编码内切木聚糖酶的XYN2基因，编码ß的xlnD基因这些基因的共表达也通过在HpGAP启动子控制下融合到多形汉逊酵母染色体中而得到，所得转化体能够在补充有桦木木聚糖的基本培养基上生长单独的碳源成功表达木聚糖酶导致能够发酵桦木木聚糖的受体菌株到48℃的乙醇。此外，在多克隆多因子菌株中共表达过去基因，其过表达丙酮酸脱羧酶基因进一步改善了乙醇生产。

78. 发明公开

EP3388524A1 ENZYMATIC METHOD FOR PRODUCING 2-HYDROXY-4-METHYLMERCAPTOBUTANOIC ACID (MHA) 失效转让
公开(公告)号：EP3388524A1
公开(公告)日：2018-10-17
申请号：EP18165674.5
申请日：2018-04-04
申请人： Evonik Degussa GmbH
发明人： Skerra, Arne , Martin, Julia , Jakob, Harald , Fischer, Daniel
IPC分类号： C12P7/42 , C12P11/00
CPC分类号： C12P11/00 , C12P7/42 , C12Y101/01 , C12Y101/01258 , C12Y117/01 , C12Y401/01001 , C12Y401/01043 , C12Y401/01072
摘要： The invention relates to an enzymatic method for producing 2-hydroxy-4-methylmercaptobutanoic acid (HMBA, MHA) from 3-methylthio-propanal (3-methylmercaptopropanal (MMP) or "methional") and carbon dioxide using a decarboxylase (EC 4.1.1), its corresponding cofactor, an alcohol dehydrogenase (EC 1.1.1) and NADH or NADPH.

79. 发明授权

EP3027733B1 3-HYDROXYPROPIONIC ACID PRODUCTION BY RECOMBINANT YEASTS EXPRESSING AN INSECT ASPARTATE 1-DECARBOXYLASE 有权
公开(公告)号：EP3027733B1
公开(公告)日：2018-09-12
申请号：EP14752713.9
申请日：2014-07-31
申请人： Novozymes A/S
发明人： BARNHART, Michelle , NEGRETE-RAYMOND, Ana , FRIAS, Janice , BARBIER, Gui , CATLETT, Michael
IPC分类号： C12N1/16 , C12N9/88 , C12P7/40 , C12P7/42
CPC分类号： C12P7/42 , C12N1/16 , C12N9/88 , C12P7/40 , C12Y401/01001 , C12Y401/01011
摘要： Provided herein are recombinant yeast cells having an active 3-Hydroxypropionic Acid (3-HP) pathway and further comprising a heterologous polynucleotide encoding an aspartate 1-decarboxylase (ADC) of the Class Insecta, Bivalvia, Branchioporia, Gastropoda, or Leptocardii. Also described are methods of using the recombinant yeast cells to produce 3-HP and acrylic acid.

80. 发明公开

EP3309247A1 MICROORGANISM HAVING IMPROVED PRODUCTION OF LACTIC ACID AND METHOD FOR PRODUCING LACTIC ACID BY USING SAME 审中-公开
标题翻译：具有改进的乳酸生产的微生物及使用相同方法生产乳酸的方法
公开(公告)号：EP3309247A1
公开(公告)日：2018-04-18
申请号：EP15895034.5
申请日：2015-06-12
申请人： Cj Cheiljedang Corporation
发明人： YANG, Eun Bin , LEE, Tae Hee , KIM, Seon Hye , SONG, Gyuhyeon , HA, Cheol Woong , NA, Kyungsu , YANG, Young Lyeol , KANG, Min Sun , LEE, Hyo Hyoung
IPC分类号： C12N1/21 , C12N9/02 , C12P7/56
CPC分类号： C12P7/56 , C12N1/18 , C12N9/0008 , C12N9/88 , C12N9/93 , C12Y102/01003 , C12Y401/01001 , C12Y602/01001
摘要： The present invention relates to Saccharomyces sp. capable of producing lactic acid with a decreased activity of pyruvate decarboxylase (PDC) and increased activities of aldehyde dehydrogenase (ALD) and acetyl-CoA synthetase (ACS), and a method of producing lactic acid from the culture medium obtained by culturing the microorganism.
摘要翻译：本发明涉及酵母属（Saccharomyces sp。）。其能够产生丙酮酸脱羧酶（PDC）活性降低并且醛脱氢酶（ALD）和乙酰-CoA合成酶（ACS）活性增加的乳酸，以及通过培养微生物获得的培养基生产乳酸的方法。

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