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71. 发明授权

US08088613B2 Nitrilases, nucleic acids encoding them and methods for making and using them 有权
标题翻译：腈水解酶，编码它们的核酸和制备和使用它们的方法
公开(公告)号：US08088613B2
公开(公告)日：2012-01-03
申请号：US12773317
申请日：2010-05-04
申请人： Mark Burk , Kelly Chatman , Grace Desantis , Bob Farwell , Jay M. Short , Kelvin Wong
发明人： Mark Burk , Kelly Chatman , Grace Desantis , Bob Farwell , Jay M. Short , Kelvin Wong
IPC分类号： C12N9/78 , C12N15/00 , C12N1/20 , C12P21/04 , C12Q1/00 , C07H21/04
CPC分类号： C12N9/78 , C12N15/00 , C12P7/42 , C12P13/002 , C12P13/04 , C12P41/006 , C12Y305/05001 , Y02P20/52
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
摘要翻译：本发明涉及腈水解酶和编码腈水解酶的核酸。另外还提供了设计新腈水解酶的方法及其使用方法。在增加的pH和温度下，腈水解酶具有增加的活性和稳定性。

72. 发明授权

US07651849B2 Nitrilases 有权
标题翻译：腈水解酶
公开(公告)号：US07651849B2
公开(公告)日：2010-01-26
申请号：US12397204
申请日：2009-03-03
申请人： Grace DeSantis , Ellen Chi , Jennifer Ann Chaplin , Aileen Milan , Jay M. Short , David Paul Weiner , Mark Madden , Darcy Madden, legal representative , Mark J. Burk , Dan E. Robertson
发明人： Grace DeSantis , Ellen Chi , Jennifer Ann Chaplin , Aileen Milan , Jay M. Short , David Paul Weiner , Mark Madden , Mark J. Burk , Dan E. Robertson
IPC分类号： C12N9/78 , C12N15/00 , C12N1/20 , C12P21/04 , C12Q1/00 , A61K38/00 , C07H21/04 , C12Q1/68 , C07H21/02
CPC分类号： C12P7/42 , C12N9/78 , C12N15/00 , C12N15/70 , C12N15/74 , C12N15/81 , C12N15/815 , C12P7/40 , C12P13/004 , C12P13/04 , C12P21/00 , C12P41/006
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
摘要翻译：本发明涉及腈水解酶和编码腈水解酶的核酸。另外还提供了设计新腈水解酶的方法及其使用方法。在增加的pH和温度下，腈水解酶具有增加的活性和稳定性。

73. 发明授权

US07608445B1 Nitrilases, nucleic acids encoding them and methods for making and using them 失效
标题翻译：腈水解酶，编码它们的核酸和制备和使用它们的方法
公开(公告)号：US07608445B1
公开(公告)日：2009-10-27
申请号：US10514004
申请日：2003-05-15
申请人： Grace Desantis , Jay M. Short , Mark J. Burk , Kelvin Wong , Robert Farwell , Kelly Chatman
发明人： Grace Desantis , Jay M. Short , Mark J. Burk , Kelvin Wong , Robert Farwell , Kelly Chatman
IPC分类号： C12N9/78 , C12N15/00 , C12N1/20 , C12P21/04 , C12Q1/00 , A61K38/00 , C07H21/04 , C12Q1/68 , C07H21/02
CPC分类号： C12N9/78 , C12P7/42 , C12P13/04 , C12P41/006
摘要： The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
摘要翻译：本发明涉及腈水解酶和编码腈水解酶的核酸。另外还提供了设计新腈水解酶的方法及其使用方法。在增加的pH和温度下，腈水解酶具有增加的活性和稳定性。

74. 发明申请

US20090130718A1 Gene site saturation mutagenesis 审中-公开
标题翻译：基因位点饱和诱变
公开(公告)号：US20090130718A1
公开(公告)日：2009-05-21
申请号：US11285302
申请日：2005-11-22
申请人： Jay M. Short
发明人： Jay M. Short
IPC分类号： C12P19/34
CPC分类号： C07K14/445 , A61K39/00 , A61K2039/53 , C07K16/00 , C07K16/005 , C07K2317/565 , C12N9/00 , C12N9/14 , C12N9/16 , C12N15/102 , C12N15/1027 , C12N15/1034
摘要： A method for producing progeny polynucleotides and polypeptides by Gene Site Saturation Mutagenesis (GSSM). The method provides a set of degenerate primers corresponding to codons of a template polynucleotide, and performs polymerase elongation to produce progeny polynucleotides, which contain sequences corresponding to the degenerate primers. The progeny polynucleotides can be expressed and screened for directed evolution.
摘要翻译：通过基因位点饱和诱变（GSSM）产生后代多核苷酸和多肽的方法。该方法提供了一组对应于模板多核苷酸的密码子的简并引物，并进行聚合酶延伸以产生含有对应于简并引物的序列的后代多核苷酸。可以表达和筛选后代多核苷酸用于定向进化。

75. 发明授权

US07452706B2 Recombinant bacterial phytases and uses thereof 有权
标题翻译：重组细菌植酸酶及其用途
公开(公告)号：US07452706B2
公开(公告)日：2008-11-18
申请号：US10034985
申请日：2001-12-21
申请人： Jay M. Short , Keith A. Kretz , Dan E. Robertson
发明人： Jay M. Short , Keith A. Kretz , Dan E. Robertson
IPC分类号： C12N9/16 , C12Q1/44 , A23J1/00 , A61K38/46 , C07K14/00 , C12P21/02 , C12N15/00 , C12N1/21 , C12N1/15 , C12N5/10 , C12N1/19 , C07H21/00
CPC分类号： A21D8/042 , A01K2217/05 , A23J3/14 , A23K10/30 , A23K20/189 , A23L5/25 , A23L7/107 , A23L11/33 , A23L29/06 , A23V2002/00 , A61K38/00 , C12N9/16 , C12Y301/03008 , C12Y301/03026 , A23V2300/21
摘要： A purified recombinant phytase enzyme derived from Escherichia coli B. The enzyme has a molecular weight of about 47.1 kilodaltons and has phytase activity. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In particular, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.
摘要翻译：来自大肠杆菌B的纯化重组植酸酶。该酶具有约47.1千道尔顿的分子量并具有植酸酶活性。该酶可以由天然或重组宿主细胞产生，并且可以用于有助于在需要时消化植酸盐。特别地，本发明的植酸酶可用于食品中以改善富含植酸盐的成分的摄食量。

76. 发明授权

US07416874B2 Recombinant bacterial phytases and uses thereof 有权
公开(公告)号：US07416874B2
公开(公告)日：2008-08-26
申请号：US10430356
申请日：2003-05-05
申请人： Jay M. Short
发明人： Jay M. Short
IPC分类号： C07H21/00 , C12N5/10 , C12N1/21 , C12N15/00 , C12N5/06 , C12N1/15 , C12N1/19 , C12Q1/68 , A01H1/06 , C07K14/00 , C12N9/16 , C12P21/00
CPC分类号： A21D8/042 , A01K2217/05 , A23J3/14 , A23K10/30 , A23K20/189 , A23L5/25 , A23L7/107 , A23L11/33 , A23L29/06 , A23V2002/00 , A61K38/00 , C12N9/16 , C12Y301/03008 , C12Y301/03026 , A23V2300/21
摘要： A purified recombinant phytase enzyme derived from Escherichia coli B. The enzyme has a molecular weight of about 47.1 kilodaltons and has phytase activity. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In particular, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.

77. 发明授权

US07018793B1 Combinatorial screening of mixed populations of organisms 失效
公开(公告)号：US07018793B1
公开(公告)日：2006-03-28
申请号：US09663620
申请日：2000-09-15
申请人： Jay M. Short
发明人： Jay M. Short
IPC分类号： C12Q1/68
CPC分类号： C12N9/00 , C12N9/16 , C12N9/2445 , C12N15/102 , C12N15/1027 , C12N15/1034 , C12N15/1093 , C12Y302/01021
摘要： Provided is a method of screening gene libraries derived from a mixed population of organisms for a bioactivity or biomolecule of interest. The mixed population of organisms can be a cultured population or an uncultured population from, for example, the environment. Also provided are methods of screening isolates or enriched populations of organisms, which isolates include a population that is spatially, temporally, or hierarchical, for example, of a particular species, genus, family, or class of organisms. Identified clones containing a biomolecule or bioactivity of interest can be further variegated or the DNA contained in the clone can be variegated to create novel biomolecules or bioactivities of interest.

78. 发明授权

US06764835B2 Saturation mutageneis in directed evolution 有权
标题翻译：定向进化中的饱和诱变
公开(公告)号：US06764835B2
公开(公告)日：2004-07-20
申请号：US10309587
申请日：2002-12-04
申请人： Jay M. Short
发明人： Jay M. Short
IPC分类号： C12P2106
CPC分类号： C07K14/445 , A61K39/00 , A61K2039/53 , C12N9/00 , C12N9/16 , C12N15/102 , C12N15/1027 , C12N15/1034 , C12N15/1058
摘要： Disclosed is a rapid and facilitated method of producing from a parentlal template polynucleotide, a set of mutagenized progeny polynculeotides whereby at each original codon position there is produced at least one substitute codon encoding each of the 20 naturally encoded amino acids. Accordingly, there is also provided a method of producing from a parental template polypeptide, a set of mutagenized progeny polypeptides wherein each of the 20 naturally encoded amino acids is represented at each original amino acid position. The method provided is termed site-saturation mutagenesis, or simply saturation mutagenesis, and can be used in combination with other mutagenization processes, such as, for example, a process wherein two or more related polynucleotides are introduced into a suitable host cell such that a hybrid polynucleotide is generated by recombination and reductive reassortment. Also provided are vector and expression vehicles incuding such polynucleotides, polypeptides expressed by the hybrid polynucleotides and a method for screening for hybrid polypeptides.
摘要翻译：公开了一种由亲本模板多核苷酸，一组诱变的后代多核苷酸产生的快速和便利的方法，其中在每个原始密码子位置产生编码20个天然编码氨基酸中的每一个的至少一个替代密码子。因此，还提供了从亲本模板多肽，一组诱变的后代多肽生产其中每个原始氨基酸位置上表示20个天然编码氨基酸的方法。所提供的方法称为位点饱和诱变或简单的饱和诱变，并且可以与其它诱变过程组合使用，例如其中将两个或更多个相关多核苷酸引入合适的宿主细胞中的方法，使得通过重组和还原重配产生杂交多核苷酸。还提供了包含这种多核苷酸的载体和表达载体，由杂交多核苷酸表达的多肽和杂交多肽的筛选方法。

79. 发明授权

US06696275B2 End selection in directed evolution 有权
标题翻译：定向演化中的终极选择
公开(公告)号：US06696275B2
公开(公告)日：2004-02-24
申请号：US09867262
申请日：2001-05-29
申请人： Jay M. Short , Gerhard Johann Frey
发明人： Jay M. Short , Gerhard Johann Frey
IPC分类号： C12P2106
CPC分类号： C12Q1/6811 , C12N15/102 , C12N15/1058
摘要： A directed evolution process comprising novel methods for generating improved progeny molecules having desirable properties, including, for example, a method for rapid and facilitated production from a parental polynucleotide template, of a set of mutagenized progeny polynucleotides wherein at least one codon encoding each of the 20 naturally encoded amino acids is represented at each original codon position. This method, termed site-saturation mutagenesis, or simply saturation mutagenesis, is preferably based on the use of the degenerate N,N,G/T sequence. Also, a method of producing from a parental polypeptide template, a set of mutagenized progeny polypeptides wherein each of the 20 naturally encoded amino acids is represented at each original amino acid position. Also, other mutagenization processes that can be used in combination with, or in lieu of, saturation mutagenesis, including, for example: (a) assembly and/or reassembly of polynucloetide building blocks, which building blocks can be sections of genes &/or of gene families; and (b) introduction of two or more related polynucleotides into a suitable host cell such that a hybrid polynucleotide is generated by recombination and reductive reassortment. Also, vector and expression vehicles including such polynucleotides and correspondingly expressed polypeptides. Also molecular property screening methods, including a preferred method, termed end selection, comprised of using an enzyme, such as a topoisomerase, a restriction endonuclease, &/or a nicking enzyme (such as N. BstNB I), to detect a specific terminal sequence in a working polynucleotide, to produce a ligatable end thereat, and to ligate and clone the working polynucleotide.
摘要翻译：一种定向进化过程，其包括用于产生具有期望性质的改良后代分子的新方法，包括例如一组诱变后代多核苷酸的亲本多核苷酸模板的快速和便利生产的方法，其中至少一个编码在每个原始密码子位置表示20个天然编码的氨基酸。该方法，称为位点饱和诱变，或简单的饱和诱变，优选基于使用简并N，N，G / T序列。另外，从亲本多肽模板产生一组诱变的后代多肽的方法，其中在每个原始氨基酸位置表示20个天然编码氨基酸中的每一个。此外，可以与饱和诱变组合使用或代替饱和诱变使用的其它诱变过程，包括例如：（a）组合和/或重组多核苷酸结构单元，所述构建基团可以是基因的部分和/或的基因家族; 和（b）将两种或更多种相关多核苷酸引入合适的宿主细胞中，使得通过重组和还原重配产生杂交多核苷酸。而且，载体和表达载体包括这种多核苷酸和相应表达的多肽。还包括称为末端选择的分子特性筛选方法，其包括使用酶，例如拓扑异构酶，限制性内切核酸酶和/或切酶（例如N.BstNB I），以检测特异性末端序列，以在其中产生可连接的末端，并连接和克隆工作的多核苷酸。

80. 发明授权

US06677115B2 Protein activity screening of clones having DNA from uncultivated microorganisms 失效
标题翻译：具有来自未培养微生物的DNA的克隆的蛋白质活性筛选
公开(公告)号：US06677115B2
公开(公告)日：2004-01-13
申请号：US09875412
申请日：2001-06-06
申请人： Jay M. Short
发明人： Jay M. Short
IPC分类号： C12Q100
CPC分类号： C12N15/1086 , C12N15/1034 , C12N15/1079 , C12N15/1093 , C12N15/52 , C12Q1/00
摘要： Disclosed is a process of screening clones having DNA from an uncultivated microorganism for a specified protein, e.g. enzyme, activity by screening for a specified protein, e.g. enzyme, activity in a library of clones prepared by (i) recovering DNA from a DNA population derived from at least one uncultivated microorganism; and (ii) transforming a host with recovered DNA to produce a library of clones which is screened for the specified protein, e.g. enzyme, activity.
摘要翻译：公开了一种筛选具有来自未培养的微生物的DNA的克隆用于特定蛋白质的方法，例如，酶，通过筛选特定蛋白质的活性，例如酶，通过（i）从源自至少一种未培养微生物的DNA群体回收DNA的克隆文库中的活性; 和（ii）用回收的DNA转化宿主以产生筛选特定蛋白质的克隆文库，例如。酶，活性。

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