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    • 71. 发明授权
    • Mutant aspartokinase gene
    • 突变型天冬氨酸激酶基因
    • US5688671A
    • 1997-11-18
    • US532828
    • 1995-10-27
    • Masakazu SugimotoYuri OgawaTomoko SuzukiAkiko TanakaHiroshi Matsui
    • Masakazu SugimotoYuri OgawaTomoko SuzukiAkiko TanakaHiroshi Matsui
    • C12N9/12C12N15/09C12N15/54C12P13/08C12R1/13C12R1/15
    • C12N9/1217C12N9/12C12P13/08
    • Recombinant DNA replicable in microorganisms belonging to the genus Corynebacterium, which contains a DNA fragment coding for an aspartokinase .alpha.-subunit protein originating from a bacterium belonging to the genus Corynebacterium, in which synergistic feedback inhibition by L-lysine and L-threonine is substantially desensitized, and a DNA fragment coding for an aspartokinase .beta.-subunit protein originating from a bacterium belonging to the genus Corynebacterium, in which synergistic feedback inhibition by L-lysine and L-threonine is substantially desensitized, is introduced into a microorganism belonging to the genus Corynebacterium. Thus a transformant having enhanced production and excretion speeds of L-lysine is obtained. The transformant is cultivated in an appropriate medium, and produced L-lysine is separated.
    • PCT No.PCT / JP93 / 01809 Sec。 371 1995年10月27日第 102(e)日期1995年10月27日PCT提交1993年12月14日PCT公布。 出版物WO94 / 25605 日期1994年11月10日在棒状杆菌属的微生物中可复制的重组DNA,其含有编码源自属于棒状杆菌属的细菌的天冬氨酸激酶α亚基蛋白的DNA片段,其中L-赖氨酸和L的协同反馈抑制 - 苏氨酸基本脱敏,编码源于属于棒状杆菌属的细菌的天冬氨酸激酶β亚基蛋白的DNA片段,其中L-赖氨酸和L-苏氨酸的协同反馈抑制基本上脱敏,被引入微生物 属于棒杆菌属。 因此获得具有增强的L-赖氨酸的产生和排泄速度的转化体。 将转化体在合适的培养基中培养,分离出L-赖氨酸。