专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

71. 发明授权

US07109316B2 Oligonucleotide tagged nucleoside triphosphates (OTNTPs) for genetic analysis 失效
标题翻译：寡核苷酸标记核苷三磷酸（OTNTP）用于遗传分析
公开(公告)号：US07109316B2
公开(公告)日：2006-09-19
申请号：US10226734
申请日：2002-08-23
申请人： Shiv Kumar , Anup Sood , Sudhakar Rao , John Nelson
发明人： Shiv Kumar , Anup Sood , Sudhakar Rao , John Nelson
IPC分类号： C07H21/02 , C07H21/04 , C07H19/04 , C12Q1/68
CPC分类号： C07H19/04
摘要： Oligonucleotide tagged nucleoside triphosphates, OTNTPs, which are substrates for polymerases and or terminal nucleotidyl transferases are provided as well as methods of making these OTNTPs. Further provided are OTNTPs with fluorescent dyes including energy transfer dyes, attached to the oligonucleotide chain, OTNTPs with unnatural bases incorporated in the oligonucleotide sequence and methods for incorporating these OTNTPs in DNA or RNA. Also provided are methods for using the oligonucleotides on OTNTPs for amplifying the oligo sequence on the OTNTP using an amplification method described above. Further provided are reactive bifunctional amidites, methods of making these compounds and methods for detecting single nucleotide polymorphisms using the above OTNTPs. Methods for detecting differential gene expression using the OTNTPs and methods of separating specifically modified DNA or RNA using the OTNTPs are also provided.
摘要翻译：提供了寡聚核苷酸标记的核苷三磷酸酯，OTNTP，其是用于聚合酶和末端核苷酸转移酶的底物，以及制备这些OTNTP的方法。还提供了含有荧光染料的OTNTP，其包括与寡核苷酸链连接的能量转移染料，掺入寡核苷酸序列中的非天然碱基的OTNTP以及将这些OTNTP引入DNA或RNA中的方法。还提供了使用寡核苷酸在OTNTP上使用上述扩增方法在OTNTP上扩增寡核苷酸序列的方法。还提供了反应性双官能脒，制备这些化合物的方法和使用上述OTNTP检测单核苷酸多态性的方法。还提供了使用OTNTP检测差异基因表达的方法和使用OTNTP分离特异性修饰的DNA或RNA的方法。

72. 发明授权

US07052839B2 Terminal-phosphate-labeled nucleotides and methods of use 有权
公开(公告)号：US07052839B2
公开(公告)日：2006-05-30
申请号：US10113030
申请日：2002-04-01
申请人： John Nelson , Carl Fuller , Anup Sood , Shiv Kumar
发明人： John Nelson , Carl Fuller , Anup Sood , Shiv Kumar
IPC分类号： C12Q1/68
CPC分类号： C07H19/10 , C07H19/20 , C07H21/00 , C12Q1/6816 , C12Q1/6851
摘要： The present invention describes methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases. The methods provided by this invention utilize a nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogue which has a colorimetric dye, chemiluminescent, or fluorescent moiety, a mass tag or an electrochemical tag attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Cleavage of the polyphosphate product of phosphoryl transfer via phosphatase leads to a detectable change in the label attached thereon. When the polymerase assay is performed in the presence of a phosphatase, there is provided a convenient method for real-time monitoring of DNA or RNA synthesis and detection of a target nucleic acid.

73. 发明授权

US5280119A Heterocyclic amine-boranes, and method of inhibiting DNA topoisomerase activity and/or combatting inflammation, hyperlipidemia, and/or neoplasia using amine-borane compounds 失效
标题翻译：杂环胺 - 硼烷和使用胺 - 硼烷化合物抑制DNA拓扑异构酶活性和/或抵抗炎症，高脂血症和/或瘤形成的方法
公开(公告)号：US5280119A
公开(公告)日：1994-01-18
申请号：US786279
申请日：1991-11-01
申请人： Bernard F. Spielvogel , Anup Sood , Iris H. Hall
发明人： Bernard F. Spielvogel , Anup Sood , Iris H. Hall
IPC分类号： C07F5/02 , A61K33/22 , A61K43/00
CPC分类号： C07F5/022 , C07F5/027
摘要： Heterocyclic amine-borane compounds of the formula: ##STR1## wherein: A is a heterocyclic amine moiety selected from the group consisting of piperidine, substituted piperidine, piperazine, substituted piperazine, imidazole, substituted imidazole, pyrazole, substituted pyrazole, pyrazine, substituted pyrazine, pyrrole, substituted pyrrole, pyrrolidine, substituted pyrrolidine, indole, substituted indole, indoline, substituted indoline, quinoline, substituted quinoline, isoquinoline, substituted isoquinoline, thiazole, substituted thiazole, oxazole, substituted oxazole, thiazolidine, and substituted thiazolidine, wherein substitution is at any or all substituent positions, and comprises substituents selected from the group consisting of alkyl (preferably C.sub.1 -C.sub.10 linear or branched alkyl), alkylaryl, aryl, aralkyl, nitro, alkoxy, hydroxy, cyano, thiol, and halo;R.sub.1 is selected from H, alkyl (preferably C.sub.1 -C.sub.10 linear or branched alkyl), alkylaryl, aryl, and arylalkyl; andR.sub.2 is selected from CN, COOH, COOR.sub.3, and CONHR.sub.3 where R.sub.3 is selected from H, alkyl (preferably C.sub.1 -C.sub.10 linear or branched alkyl), alkylaryl, aryl, aralkyl.The heterocyclic amine-borane compounds of the invention are bioactive in character, variously exhibiting anti-tumor, anti-inflammatory, and anti-hyperlipidemic activity. In another aspect, the invention relates to a method of inhibiting enzyme activity (e.g., DNA topoisomerase II, PRPP amidotransferase, IMP dehydrogenase, dihydrofolate reductase, and/or ribonucleotide reductase) in an in vitro or in vivo system, comprising administering to the system an enzyme-inhibitingly effective amount of a Lewis-base borane adduct, such as for example a heterocyclic amine-borane compound of the foregoing formula.
摘要翻译：式（I）的杂环胺 - 硼烷化合物，其中：A是杂环胺部分; R1选自H，烷基，烷基芳基，芳基和芳基烷基; 并且R 2选自CN，COOH，COOR 3和CONHR 3，其中R 3选自H，烷基，烷基芳基，芳基，芳烷基。本发明的杂环胺 - 硼烷化合物是具有生物活性的，各种表现出抗肿瘤，抗炎和抗高血脂活性。本发明还包括在体外或体内系统中抑制酶活性的方法（例如，DNA拓扑异构酶II，PRPP氨基转移酶，IMP脱氢酶，二氢叶酸还原酶和/或核糖核苷酸还原酶），包括向系统施用酶抑制有效量的路易斯碱硼烷加合物，例如上述式的杂环胺 - 硼烷化合物。

74. 发明授权

US5177198A Process for preparing oligoribonucleoside and oligodeoxyribonucleoside boranophosphates 失效
标题翻译：制备寡核苷酸和寡糖氧基二硼磷酸酯的方法
公开(公告)号：US5177198A
公开(公告)日：1993-01-05
申请号：US443781
申请日：1989-11-30
申请人： Bernard F. Spielvogel , Anup Sood , Iris H. Hall , Barbara Ramsay Shaw
发明人： Bernard F. Spielvogel , Anup Sood , Iris H. Hall , Barbara Ramsay Shaw
IPC分类号： A61K31/69 , A61K31/70 , A61K31/7135 , A61P3/06 , A61P29/00 , A61P35/00 , C07H21/00 , C07H21/04 , C07H23/00 , C12N15/113 , C12Q1/68
CPC分类号： C12N15/113 , C07H21/00 , C12Q1/6876 , C12N2310/31 , C12N2310/312 , C12N2310/315 , C12N2310/321 , C12N2310/353 , Y02P20/55
摘要： A process of making oligoribonucleoside and deoxyribonucleoside boranophosphates and salts thereof is disclosed. The process comprises the steps of condensing a ribonucleotide or deoxyribonucleotide with a nucleoside phosphitylating agent, wherein the phosphitylating agent includes a 5' -OH group protected by an acid-labile protecting group and a moiety bonded to the phosphorus atom therein that is a stronger proton acceptor than the 5'-OH group of the ribonucleotide or deoxyribonucleotide to form a reaction intermediate, then oxidizing the reaction intermediate so formed with a compound comprising a Lewis base and a boron-containing substituent, wherein the Lewis base is a weaker electron donor than the phosphite phosphorus of the reaction intermediate, to form a ribonucleotide or deoxyribonucleotide boranophosphate. These steps are repeated on the nucleotide boranophosphate to form an oligonucleotide boranophosphate.
摘要翻译：公开了制备寡核糖核苷和脱氧核糖核苷硼烷磷酸盐及其盐的方法。该方法包括将核糖核苷酸或脱氧核糖核苷酸与核苷磷酸化沉淀剂缩合的步骤，其中磷酸化剂包括被酸不稳定保护基团保护的5'-OH基团和与其中的磷原子键合的部分，其是更强的质子受体比核糖核苷酸或脱氧核糖核苷酸的5'-OH基团形成反应中间体，然后用包含路易斯碱和含硼取代基的化合物氧化形成的反应中间体，其中路易斯碱比较弱的电子给体比反应中间体的亚磷酸酯磷，形成核糖核苷酸或脱氧核糖核苷酸硼磷酸盐。在核苷酸硼酸盐上重复这些步骤以形成寡核苷酸硼磷酸盐。

75. 发明授权

US5130302A Boronated nucleoside, nucleotide and oligonucleotide compounds, compositions and methods for using same 失效
标题翻译：硼氢化核苷，核和寡核苷酸化合物，组合物及其使用方法
公开(公告)号：US5130302A
公开(公告)日：1992-07-14
申请号：US453311
申请日：1989-12-20
申请人： Bernard F. Spielvogel , Anup Sood , Iris H. Hall , Barbara Ramsay Shaw
发明人： Bernard F. Spielvogel , Anup Sood , Iris H. Hall , Barbara Ramsay Shaw
IPC分类号： A61K31/70 , A61K31/7135 , A61P3/08 , A61P35/00 , A61P37/08 , C07D473/00 , C07D473/18 , C07D473/34 , C07F5/02 , C07H19/04 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07H23/00
CPC分类号： C07D473/00 , C07D473/18 , C07D473/34 , C07F5/02 , C07H19/04 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07H23/00
摘要： A novel class of pharmaceutically active boronated nucleosides are provided. The nucleosides are boronated at a ring nitrogen of the purine or pyrimidine or analogues thereof. Also provided are phosphate esters of these nucleosides and oligomers thereof. Methods of making and using the boronated nucleosides are also disclosed.
摘要翻译：提供了一类新颖的药学活性硼化核苷。核苷在嘌呤或嘧啶或其类似物的环氮上硼化。还提供了这些核苷的磷酸酯及其低聚物。还公开了制备和使用硼化核苷的方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式