专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

71. 发明授权

US06677115B2 Protein activity screening of clones having DNA from uncultivated microorganisms 失效
标题翻译：具有来自未培养微生物的DNA的克隆的蛋白质活性筛选
公开(公告)号：US06677115B2
公开(公告)日：2004-01-13
申请号：US09875412
申请日：2001-06-06
申请人： Jay M. Short
发明人： Jay M. Short
IPC分类号： C12Q100
CPC分类号： C12N15/1086 , C12N15/1034 , C12N15/1079 , C12N15/1093 , C12N15/52 , C12Q1/00
摘要： Disclosed is a process of screening clones having DNA from an uncultivated microorganism for a specified protein, e.g. enzyme, activity by screening for a specified protein, e.g. enzyme, activity in a library of clones prepared by (i) recovering DNA from a DNA population derived from at least one uncultivated microorganism; and (ii) transforming a host with recovered DNA to produce a library of clones which is screened for the specified protein, e.g. enzyme, activity.
摘要翻译：公开了一种筛选具有来自未培养的微生物的DNA的克隆用于特定蛋白质的方法，例如，酶，通过筛选特定蛋白质的活性，例如酶，通过（i）从源自至少一种未培养微生物的DNA群体回收DNA的克隆文库中的活性; 和（ii）用回收的DNA转化宿主以产生筛选特定蛋白质的克隆文库，例如。酶，活性。

72. 发明授权

US06602675B2 High throughput screening of mycelia for bioactivities or biomolecules 有权
标题翻译：用于生物活性或生物分子的菌丝体的高通量筛选
公开(公告)号：US06602675B2
公开(公告)日：2003-08-05
申请号：US09848083
申请日：2001-05-03
申请人： Jay M. Short , Martin Keller
发明人： Jay M. Short , Martin Keller
IPC分类号： G01N33569
CPC分类号： C40B40/02 , C12N15/1037 , C12N15/1055 , C12Q1/6811
摘要： Disclosed is a process for identifying clones having a specified activity of interest, which process comprises (i) generating one or more expression libraries derived from nucleic acid directly isolated from the environment; and (ii) screening said libraries utilizing a fluorescence activated cell sorter to identify said clones. More particularly, this is a process for identifying clones having a specified activity of interest by (i) generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment; (ii) exposing said libraries to a particular substrate or substrates of interest; and (iii) screening said exposed libraries utilizing a fluorescence activated cell sorter to identify clones which react with the substrate or substrates. Also provided is a process for identifying clones having a specified activity of interest by (i) generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment; and (ii) screening said exposed libraries utilizing an assay requiring co-encapsulation, a binding event or the covalent modification of a target, and a fluorescence activated cell sorter to identify positive clones.
摘要翻译：公开了用于鉴定具有特定的感兴趣活性的克隆的方法，该方法包括（i）产生一种或多种源自从环境直接分离的核酸的表达文库; 和（ii）使用荧光激活细胞分选仪筛选所述文库以鉴定所述克隆。更具体地说，这是通过（i）产生一种或多种从环境中直接或间接分离的核酸产生的表达文库来鉴定具有特定活性的克隆的方法; （ii）将所述文库暴露于感兴趣的特定底物或底物; 和（iii）使用荧光激活的细胞分选仪筛选所述暴露的文库，以鉴定与底物或底物反应的克隆。还提供了通过（i）生成从环境直接或间接分离的核酸产生的一种或多种表达文库来鉴定具有特定活性的克隆的方法; 和（ii）使用需要共包封，结合事件或靶共价修饰的测定法和荧光激活的细胞分选器筛选所述暴露的文库以鉴定阳性克隆。

73. 发明授权

US06566050B2 Enzyme kits and libraries 有权
公开(公告)号：US06566050B2
公开(公告)日：2003-05-20
申请号：US09861267
申请日：2001-05-18
申请人： Jay M. Short
发明人： Jay M. Short
IPC分类号： C12Q1100
CPC分类号： C12N15/1093 , C12N15/1086 , C12N15/52 , C12Q1/00
摘要： Recombinant enzyme libraries and kits where a plurality of enzymes are each characterized by different physical and/or chemical characteristics and classified by common characteristics. The characteristics are determined by screening of recombinant enzymes expressed by a DNA library produced from various microorganisms.

74. 发明授权

US06344328B1 Method for screening for enzyme activity 有权
公开(公告)号：US06344328B1
公开(公告)日：2002-02-05
申请号：US09557276
申请日：2000-04-24
申请人： Jay M. Short
发明人： Jay M. Short
IPC分类号： C12Q168
CPC分类号： C12N15/1034 , C12N9/00 , C12N9/1029 , C12N9/16 , C12N15/102 , C12Q1/6813 , C12Q2565/531 , C12Q2521/543
摘要： Disclosed is a process for identifying clones having a specified enzyme activity by screening for the specified enzyme activity in a library of clones prepared by (i) selectively isolating target DNA from DNA derived from at least one microorganism, by use of at least one probe DNA comprising at least a portion of a DNA sequence encoding an enzyme having the specified enzyme activity; and (ii) transforming a host with isolated target DNA to produce a library of clones which are screened for the specified enzyme activity.

75. 发明授权

US06171820B2 Saturation mutagenesis in directed evolution 有权
标题翻译：定向进化中的饱和诱变
公开(公告)号：US06171820B2
公开(公告)日：2001-01-09
申请号：US09246178
申请日：1999-02-04
申请人： Jay M. Short
发明人： Jay M. Short
IPC分类号： C12P2106
CPC分类号： C12N15/1058 , A61K39/00 , A61K2039/53 , C07K14/445 , C12N9/00 , C12N9/14 , C12N9/16 , C12N9/88 , C12N15/102 , C12N15/1027 , C12N15/1034 , C12Y301/11002 , Y02A50/412 , Y02A50/58
摘要： Disclosed is a rapid and facilitated method of producing from a parental template polynucleotide, a set of mutagenized progeny polynucleotides whereby at each original codon position there is produced at least one substitute codon encoding each of the 20 naturally encoded amino acids. Accordingly, there is also provided a method of producing from a parental template polypeptide, a set of mutagenized progeny polypeptides wherein each of the 20 naturally encoded amino acids is represented at each original amino acid position. The method provided is termed site-saturation mutagenesis, or simply saturation mutagenesis, and can be used in combination with other mutagenization processes, such as, for example, a process wherein two or more related polynucleotides are introduced into a suitable host cell such that a hybrid polynucleotide is generated by recombination and reductive reassortment. Also provided are vector and expression vehicles including such polynucleotides, polypeptides expressed by the hybrid polynucleotides and a method for screening for hybrid polypeptides.
摘要翻译：公开了一种从父母模板多核苷酸，一组诱变的后代多核苷酸产生的快速和便利的方法，由此在每个原始密码子位置产生编码20个天然编码氨基酸中的每一个的至少一个替代密码子。因此，还提供了从亲本模板多肽，一组诱变的后代多肽生产其中每个原始氨基酸位置上表示20个天然编码氨基酸的方法。所提供的方法称为位点饱和诱变或简单的饱和诱变，并且可以与其它诱变过程组合使用，例如其中将两个或更多个相关多核苷酸引入合适的宿主细胞中的方法，使得通过重组和还原重配产生杂交多核苷酸。还提供了载体和表达载体，包括这样的多核苷酸，由杂交多核苷酸表达的多肽和杂交多肽的筛选方法。

76. 发明授权

US06030779A Screening for novel bioactivities 失效
公开(公告)号：US06030779A
公开(公告)日：2000-02-29
申请号：US944795
申请日：1997-10-06
申请人： Jay M. Short
发明人： Jay M. Short
IPC分类号： C12N9/00 , C12N9/16 , C12N9/24 , C12N15/10 , C12Q1/68 , C12P19/34
CPC分类号： C12N15/1086 , C12N15/102 , C12N9/00 , C12N9/16 , C12N9/2402 , C12Q1/6811
摘要： Disclosed is a process for identifying clones having a specified enzyme activity by screening for the specified enzyme activity in a library of clones prepared by (i) selectively isolating target nucleic acid from nucleic acid derived from at least one microorganism, by use of at least one polynucleotide probe comprising at least a portion of a nucleic acid sequence encoding an enzyme having the specified enzyme activity; and (ii) transforming a host with isolated target nucleic acid to produce a library of clones which are screened for the specified enzyme activity.

77. 发明授权

US5286636A DNA cloning vectors with in vivo excisable plasmids 失效
标题翻译：具有体内可切除质粒的DNA克隆载体
公开(公告)号：US5286636A
公开(公告)日：1994-02-15
申请号：US856556
申请日：1992-05-21
申请人： William Huse , Joseph A. Sorge , Jay M. Short
发明人： William Huse , Joseph A. Sorge , Jay M. Short
IPC分类号： C12N15/64 , C12N15/70 , C12N15/10
CPC分类号： C12N15/70 , C12N15/64
摘要： Vectors are described that circumvent traditional DNA cloning and subcloning procedures, and that contain a unique DNA cartridge that permits both cloning of DNA directly into DNA sequences present within the cartridge, and in vivo removal and circularization of the cartridge thereby yielding an autonomously replicating structure. Because the DNA cartridge can include a wide variety of functional DNA sequences, the cloned DNA can be subjected to a plethora of molecular biological procedures without having to remove the cloned DNA from the cartridge thereby obviating the need to perform additional subcloning techniques. A particularly useful example of this type of vector is bacteriophage lambda containing the DNA cartridge.
摘要翻译：描述了可以克服传统DNA克隆和亚克隆程序的载体，并且其含有独特的DNA柱，其允许将DNA直接克隆到存在于盒内的DNA序列中，并且在体内移除和环化圆筒，从而产生自主复制的结构。因为DNA盒可以包括多种功能性DNA序列，所以克隆的DNA可以经受大量的分子生物学过程，而不必从盒中除去克隆的DNA，从而避免进行附加亚克隆技术的需要。这种载体的特别有用的实例是含有DNA盒的噬菌体λ。

78. 发明授权

US10106576B2 Methods of protein evolution 有权
公开(公告)号：US10106576B2
公开(公告)日：2018-10-23
申请号：US13810601
申请日：2011-03-04
申请人： Jay M. Short
发明人： Jay M. Short
IPC分类号： C40B30/04 , C07K1/107 , C07K1/04 , C12N15/10 , G01N33/68 , G06F19/18
摘要： The present invention is relevant to proteins and novel methods of protein evolution. The present invention further relates to methods of identifying and mapping mutant polypeptides formed from, or based upon, a template polypeptide.

79. 发明授权

US09464284B2 Mirac proteins 有权
标题翻译： Mirac蛋白
公开(公告)号：US09464284B2
公开(公告)日：2016-10-11
申请号：US13255676
申请日：2010-03-09
申请人： Jay M. Short , Hwai Wen Chang , Gerhard Frey
发明人： Jay M. Short , Hwai Wen Chang , Gerhard Frey
IPC分类号： C12P21/06 , C12N9/00 , C12N15/10 , C07K14/315 , C12N9/72 , G01N33/68 , C07K7/06 , C07K14/47 , C07K14/575 , C12N9/16 , C12N9/50 , G01N33/543 , G01N33/573 , A61K38/00
CPC分类号： C12N15/102 , A61K38/00 , C07K7/06 , C07K14/3153 , C07K14/47 , C07K14/575 , C07K14/57545 , C07K14/57563 , C12N9/16 , C12N9/50 , C12N9/6435 , C12N9/6459 , C12N9/6462 , C12N15/1058 , C12Y302/01035 , C12Y304/21007 , C12Y304/21069 , C12Y304/21073 , C12Y304/23015 , C12Y304/24029 , G01N33/54306 , G01N33/573 , G01N33/6845 , G01N33/6854 , Y02A50/473
摘要： This disclosure relates to a method of generating conditionally active biologic proteins from wild type proteins, in particular therapeutic proteins, which are reversibly or irreversibly inactivated at the wild type normal physiological conditions. For example, evolved proteins are virtually inactive at body temperature, but are active at lower temperatures.
摘要翻译：本公开涉及从野生型正常生理条件下可逆地或不可逆地灭活的野生型蛋白质，特别是治疗性蛋白质产生条件活性生物蛋白质的方法。例如，进化蛋白质在体温实际上是无活性的，但在较低温度下是活性的。

80. 再颁专利

USRE45349E1 Synthetic ligation reassembly in directed evolution 有权
公开(公告)号：USRE45349E1
公开(公告)日：2015-01-20
申请号：US11798032
申请日：2007-05-09
申请人： Jay M. Short
发明人： Jay M. Short
IPC分类号： C12P21/06 , C12Q1/68
CPC分类号： C12N15/1058 , A61K39/00 , A61K2039/53 , C07K14/445 , C12N9/14 , C12N15/102 , C12N15/1027 , C12N15/1034
摘要： Harvesting the full richness of biodiversity is instantly recognized by Diversa Corporation as a powerful means to access both novel molecules having direct commercial utility as well as molecular templates that could be retooled to acquire commercial utility. A directed evolution process for rapid and facilitated production from a progenitor polynucleotide template, of a library of mutagenized progeny polynucleotides wherein each of the 20 naturally encoded amino acids is encoded at each original codon position. This method, termed site-saturation mutagenesis, or simply saturation mutagenesis, is preferably based on the use of the degenerate N,N,G/T sequence. Also, a method of non-stochastically producing a library of chimeric nucleic acid molecules having an overall assembly order that is chosen by design. Accordingly, a set of progenitor templates, such as genes (e.g. a family of esterase genes) or genes pathways (e.g. encoding antibiotics) can be shuffled to generate a sizable library of distinct progeny polynucleotide molecules (e.g. 10100) and correspondingly encoded polypeptides. Screening of these polynucleotide libraries enables the identification of a desirable molecular species that has a desirable property, such as a specific enzymatic activity serviceable for a commercial application, or a novel antibiotic. Also, a method of retooling genes and gene pathways by the introduction of regulatory sequences, such as promoters, that are operable in an intended host, thus conferring operability to a novel gene pathway when it is introduced into an intended host. For example a novel man-made gene pathway, generated based on microbially-derived progenitor templates, that is operable in a plant cell.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式