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    • 77. 发明申请
    • Method for sex biasing spermatozoa
    • US20050114915A1
    • 2005-05-26
    • US10723268
    • 2003-11-26
    • Barb CohenMichael Morris
    • Barb CohenMichael Morris
    • A01K67/027C12N5/00C12N5/071G01N20060101
    • C12N5/0612A01K2227/101A01K2227/105C12N2517/10
    • A method is described for treating a specimen of semen containing sperm cells to increase the relative number of sperm cells of a desired sex type in a treated specimen to increase the potential for conceiving an offspring of the desired sex. A specimen of semen is treated after a predetermined time to increase the relative ability of a at least a portion of the semen to conceive an offspring of the desired sex. The treatment preferably comprises contacting the sperm cells with an agent that preferentially effects sperm cells of a selected sex type. In some preferred embodiments, the semen is separated into two components. A first component has a higher number of sperm of the desired sex type than sperm of a non desired sex type and a second component has a higher number of sperm of the non desired sex type relative to sperm of the desired sex type. In one embodiment, the separating step is performed after a predetermined percent of the sperm cells exhibit a punctate pattern, which is capable of determination by labeling the sperm cells with Koo antibody and determining the percent of cells labeled. In another embodiment, the separating step is performed after waiting for a time determined by locating a maximum in the curve obtained by plotting percent female cells determined by FISH against percent Koo positive cells, determining the time at which the maximum percent female cells occurs, and beginning the following step no earlier than about one hour before the time of the maximum percent female cells. In yet another embodiment, the separating step is performed after waiting for a period of time between about 2 hours and about 24 hours after collection of the ejaculate. Preferably, in the separating step, the sperm is contacted with a cell binding agent, permitting the sperm of the non desired sex type to preferentially bind to the cell binding agent, and the cell binding agent with preferentially bound sperm of the non desired sex type is separated from non bound sperm.
    • 80. 发明授权
    • Soft book
    • 软书
    • US06390507B1
    • 2002-05-21
    • US09886161
    • 2001-06-20
    • William DerraughMichael Morris
    • William DerraughMichael Morris
    • B42D100
    • B42D1/003
    • A soft book comprised of foam pages bound by a book binding portion and a plurality of page binding portions is presented. A front cover page, a rear cover page, and at least one page, each page may which may have a perimeter, are provided, with the pages forming a spine portion, with the book bound such that a portion of the perimeter each page may extend beyond the binding portions to provide a soft perimeter for the book so that it is safe, particularly for children. The pages of the book may include impressions or cutouts that may be made in various shapes. Cutouts may provide a puzzle-type game for children. Various binding materials may be used, preferably chosen to provide sufficient support for the flexible foam pages.
    • 呈现由书籍装订部分和多个页面装订部分所限定的泡沫页构成的软书。 提供前盖页面,后盖页面和至少一个页面,每个页面可以具有周边,页面形成脊柱部分,书限于每页的周边的一部分可以 延伸超过绑定部分以为书提供柔软的周长,使得它是安全的,特别是对于儿童。 本书的页面可能包括可以以各种形状制作的印象或切口。 切割可能为儿童提供一个拼图游戏。 可以使用各种粘合材料,优选选择为柔性泡沫页提供足够的支撑。