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71. 发明专利

AU2013352723A1 Apparatus for automatically preparing cell-free proteins and method for preparing proteins using same 未知
公开(公告)号：AU2013352723A1
公开(公告)日：2015-06-18
申请号：AU2013352723
申请日：2013-11-29
申请人： BIONEER CORP
发明人： PARK HAN OH , KIM JONG KAB , HAN JI WON , CHO YOU SANG , KIM MIN JUNG , KIM HA NEUL , LEE YANG WON , KIM II NAM
IPC分类号： C12M1/38 , C07K1/22
摘要： The present invention relates to an apparatus for automatically preparing cell-free proteins and a method for preparing proteins using the same, and more particularly, to an apparatus for automatically preparing cell-free proteins and a method for manufacturing proteins using the same, the apparatus comprising: a protein expression reaction unit including a reaction vessel comprising a plurality of dialysis tubes provided with a dialysis membrane by an open superstructure; a reaction temperature control unit for heating or cooling the protein expression reaction unit; a pipet array which includes a plurality of pipets and is capable of suctioning or discharging a solution by using the pipets; a pipet array movement unit capable of moving solutions by moving the pipet array up and down, back and forth, or left and right; a protein purification unit provided with a magnetic field application apparatus; and a multi-well plate provision unit capable providing a multi-well plate kit which supplies a solution used in the preparation of proteins. In addition, the present invention relates to the apparatus for automatically preparing cell-free proteins and the preparation method therefor, which has a pure target protein synthesizing function in which an affinity tag is removed, excluding a target protein in which an affinity tag is coupled, and a function for rapidly and automatically exchanging, by a desired elution solution, through multi-step dialysis, a leaching solution in which the resulting purified proteins are dissolved, with the desired elution solution for containing proteins such that a plurality of proteins can be simply and automatically prepared in mass. Moreover, the present invention relates to a reaction kit for automatically preparing cell-free proteins, comprising: a multi-well plate kit for storing a solution necessary for preparing proteins; and a dialysis tube provided with the dialysis membrane. The apparatus for automatically preparing cell-free proteins according to the present invention can automatically express and purify target proteins, and dialyze the proteins in the buffer solution for containing proteins thereby enabling the immediate use of the resulting purified proteins. Furthermore, the method for preparing proteins according to the present invention can continuously supply energy sources necessary for cell-free protein expressions by multi-leveled exchanges of a low molecular expression solution such that a maximum of five times more target proteins can be obtained compared with obtaining target proteins by using a conventional method for preparing cell-free proteins in an arrangement form, and since the preparation of pure target proteins by removing an unnecessary affinity tag using proteases when purifying target proteins by using magnetic particles, the present invention is very useful in protein production for industrial, medicinal and research purposes wherein circular proteins can be produced in which artificial sequences exerting negative influences on the activities and structures of a protein are removed.

72. 发明专利

CA2917320A1 RESPIRATORY DISEASE-RELATED GENE SPECIFIC SIRNA, DOUBLE-HELICAL OLIGO RNA STRUCTURE CONTAINING SIRNA, COMPOSITON CONTAINING SAME FOR PREVENTING OR TREATING RESPIRATORY DISEASE 未知
公开(公告)号：CA2917320A1
公开(公告)日：2015-01-08
申请号：CA2917320
申请日：2014-07-04
申请人： BIONEER CORP , YUHAN CORP
发明人： CHAE JEIWOOK , PARK HAN OH , YOON PYOUNG OH , HAN BORAM , KIM MI NA
IPC分类号： C12N15/113 , A61K48/00 , A61P11/00 , C07H21/02
摘要： The present invention relates to respiratory diseases, especially to gene specific siRNA related to idiopathic pulmonary fibrosis and chronic obstructive pulmonary disease (COPD), and a highly efficient double-helical oligo RNA structure containing the same, wherein the double-helical oligo RNA structure has a structure in which hydrophilic and hydrophobic materials are bonded at the both ends of the double-helical RNA (siRNA) using a simple covalent bond or a linker-mediated covalent bond to be effectively transferred into a cell, and is converted into nanoparticles by the hydrophobic interaction of the double-helical oligo RNA structure in a solution. It is desirable that the siRNA contained in the double-helical oligo RNA structure is a siRNA specific to a CTGF, Cyr61, or Plekho1, which are genes related to respiratory diseases, particularly idiopathic pulmonary fibrosis and COPD. In addition, the present invention relates to a method for producing the double-helical oligo RNA structure and a pharmaceutical composition containing the double-helical oligo RNA structure for preventing or treating respiratory diseases, particularly idiopathic pulmonary fibrosis and COPD.

73. 发明专利

AU2012353058A1 Novel oligonucleotide conjugates and use thereof 未知
公开(公告)号：AU2012353058A1
公开(公告)日：2014-07-03
申请号：AU2012353058
申请日：2012-12-14
申请人： BIONEER CORP
发明人： CHAE JEIWOOK , HAN BORAM , KIM HAN-NA , PARK HAN OH , YOON PYOUNG OH , KIM SUN GI , JUNG KWANG-JU , KWON TAEWOO , CHOI JONG DEOK , LEE SAM YOUNG , JUNG EUN-JUNG
IPC分类号： A61K47/48 , A61K9/16 , A61K48/00 , C12N15/11
摘要： The present invention provides a double helix oligo-RNA structure in which target-specific ligands are bonded to the double helix oligo-RNA structure in which high molecular compounds for improving the delivery of double helix oligo-RNA that can be valuably used in treating diseases, specifically, cancer, are covalently bonded. The present invention also provides a method for preparing the RNA structure and a target-specific double helix oligo-RNA delivery technology through said structure. Nanoparticles constituted by the double helix oligo-RNA structure to which ligands are bonded may efficiently deliver double helix oligo-RNA to a target, and thus may exhibit an activity of double helix oligo-RNA structure in a target cell even with an administration of double helix oligo-RNA of relatively lower concentration. The nanoparticles may prevent the delivery of non-specific double helix oligo-RNA to other organs and cells, and therefore, can be valuably used not only as a tool for double helix oligo-RNA for treating various diseases, specifically, cancer, but also as a new type of double helix oligo-RNA delivery system. The present invention also relates to hybrid conjugates in which a hydrophilic material and a hydrophobic material are connected to both ends of antisense oligonucleotide(ASO) through covalent bond so as to achieve in vivo stability of the ASO, and to a method for preparing the hybrid conjugates and to nanoparticles constituted by the conjugates. The conjugates between ASO and macromolecules according to the present invention may improve in vivo stability of ASO, and therefore, can efficiently deliver ASO for treatment into cells and may exhibit an activity of ASO even with an administration of a relatively lower concentration.

74. 发明专利

DK2046943T3 Mælkesyrebakterie isoleret fra modermælk med probiotisk aktivitet og inhibitorisk aktivitet over for stigning i legemsvægt 未知
公开(公告)号：DK2046943T3
公开(公告)日：2013-09-23
申请号：DK07746512
申请日：2007-05-14
申请人： BIONEER CORP
发明人： KANG JI HEE , YOU BYEUNG IL , YUN SUNG IL , PARK HAN OH
IPC分类号： C12N1/20 , A23L1/30 , A61K35/74 , C07K14/335 , C12N15/31 , C12R1/225

75. 发明专利

CA2845598A1 PROTEIN SYNTHESIS KIT, AND METHOD FOR PROTEIN EXPRESSION AND PURIFICATION USING AUTOMATIC PURIFICATION EQUIPMENT 未知
公开(公告)号：CA2845598A1
公开(公告)日：2013-03-07
申请号：CA2845598
申请日：2012-08-23
申请人： BIONEER CORP
发明人： PARK HAN OH , CHO YOU SANG , JUNG JUN HO , HAN JI WON , KIM NAM IL
IPC分类号： C07K1/32 , C07K1/14 , C12M1/18 , C12N15/09
摘要： The present invention relates to a method for producing proteins. According to the method for producing proteins, using an automatic biological material refining apparatus provided with: a well plate kit; a heating part; and a magnetic field applying part, a plurality of target proteins may be obtained more quickly and simply compared to obtaining the target proteins using a protein expression/extraction method through an existing conventional cell culture. Thus, the present invention exhibits the advantage of a reproducible synthesis efficiency for proteins being improved since no deviation is generated between reaction wells.

76. 发明专利

CA2765263A1 COMPOUND FOR INHIBITING ACTIVITY OF RIBONUCLEASE, AND CONTAINER FOR STORING NUCLEIC ACID CONTAINING THE SAME 未知
公开(公告)号：CA2765263A1
公开(公告)日：2010-12-16
申请号：CA2765263
申请日：2009-06-12
申请人： BIONEER CORP
发明人： KIM JONG HOON , PARK HWANGSEO , PARK HAN OH
IPC分类号： C07D403/12 , C07D403/02 , C07D403/08
摘要： The present invention relates to a compound for effectively inhibiting the activity of ribonuclease which promotes the decomposition of collected ribonucleic acid, and a container for storing a sample containing the same. The compound for inhibiting the activity of ribonuclease and the container for storing a sample of the present invention can be effectively used for the storage of ribonucleic acid during extraction or after extracted.

77. 发明专利

AU2009263158A1 Thermal cycling reaction block and continuous real-time monitoring apparatus using the same 未知
公开(公告)号：AU2009263158A1
公开(公告)日：2009-12-30
申请号：AU2009263158
申请日：2009-06-23
申请人： BIONEER CORP
发明人： CHOI IL KYU , PARK HANEE , PARK HAN OH
IPC分类号： C12M1/38
摘要： Disclosed is real-time monitoring apparatus comprising a thermal cycling reaction block having heating block which is formed of a hollow part and divided by an insulating layer, and a capillary tube through which a sample is flowed in and/or out and which is wound on the heating block so that the different temperatures are transferred and thus reaction cycle is repeatedly performed; a light source; a band pass filter; a condensing lens; a beam splitter; a reflecting mirror which is rotatably connected with a motor so as to transfer the excitation light reflected from the beam splitter to the capillary tube and reflect the fluorescence generated from the sample in the capillary tube; and a fluorescence detecting part.

78. 发明公开

EP3073273A4 MICRO CHAMBER PLATE 审中-公开
标题翻译： MIKROKAMMERPLATTE
公开(公告)号：EP3073273A4
公开(公告)日：2017-08-16
申请号：EP14863421
申请日：2014-11-12
申请人： BIONEER CORP
发明人： SONG GU-YOUNG , PARK HAN OH , JANG WON-SEOK , BAE JUNG A
IPC分类号： G01N37/00 , B01L3/00 , G01N1/36 , G01N35/00
CPC分类号： B01L3/50853 , B01L2200/0689 , B01L2200/12 , B01L2200/142 , B01L2300/041 , B01L2300/044 , B01L2300/0829 , B01L2300/0848 , B01L2300/12 , C12Q1/686 , G01N35/028 , G01N2035/00277
摘要： The present invention relates to a microchamber plate, and more particularly, to a microchamber plate having microchamber holes formed using a flowable film. Thus, samples can be injected into the microchamber holes in a smoother manner compared to when samples are injected using a vacuum and/or centrifugal force. In addition, bubbles and excess samples in the microchamber holes can be efficiently discharged, making it possible to perform reaction and analysis in a more accurate and efficient manner.
摘要翻译：微室板技术领域本发明涉及一种微室室板，更具体地涉及具有使用可流动膜形成的微室孔的微室室板。因此，与使用真空和/或离心力注射样品时相比，样品可以以更平滑的方式注入微型腔室孔中。另外，微腔孔中的气泡和过量样品可以被有效地排出，使得可以以更准确和有效的方式进行反应和分析。

79. 发明公开

EP3019612A4 LIVER CANCER RELATED GENES-SPECIFIC siRNA, DOUBLE-STRANDED OLIGO RNA MOLECULES COMPRISING THE siRNA, AND COMPOSITION FOR PREVENTING OR TREATING CANCER COMPRISING THE SAME 审中-公开
标题翻译： LEBERKREBS-VERWANDTE GENSPEZIFISCHE SIRNA，DOPPELSTRÄNGIGEOLIGO-RNA-MOLEKÜLEMIT DER SIRNA UND ZUSAMMENSETZUNG ZURPRÄVENTIONODER BEHANDLUNG VON KREBS DAMIT
公开(公告)号：EP3019612A4
公开(公告)日：2017-06-28
申请号：EP14823194
申请日：2014-07-09
申请人： BIONEER CORP , SANOFI-AVENTIS KOREA CO LTD
发明人： CHAE JEIWOOK , YOON PYOUNG OH , KIM HAN-NA , PARK HAN OH , HAN BORAM , KO YOUNGHO , CHOI GI-EUN , KIM JAE EUN
IPC分类号： C12N15/113 , A61K31/7088 , A61K48/00 , A61P35/00
CPC分类号： C12N15/1137 , A61K9/1075 , A61K31/713 , A61K47/6907 , C12N15/113 , C12N15/1135 , C12N2310/14 , C12N2310/31 , C12N2310/351 , C12N2320/30

80. 发明公开

EP2297294A4 THERMAL CYCLING REACTION BLOCK AND CONTINUOUS REAL-TIME MONITORING APPARATUS USING THE SAME 有权
标题翻译：温度应激反应BLOCK和实际持续时间监控
公开(公告)号：EP2297294A4
公开(公告)日：2016-09-07
申请号：EP09770367
申请日：2009-06-23
申请人： BIONEER CORP
发明人： PARK HAN OH , PARK HANEE , CHOI IL KYU
IPC分类号： B01L7/00 , G01N21/64
CPC分类号： B01L7/525 , B01L2300/0838 , B01L2300/0861 , B01L2300/1805 , G01N21/64 , G01N2021/6467

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