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61. 发明申请

US20040077016A1 Mutant kanamycin nucleotidyltransferase and a method of screening thermophilic bacteria using the same 审中-公开
标题翻译：突变型卡那霉素核苷酸转移酶和使用其的嗜热细菌筛选方法
公开(公告)号：US20040077016A1
公开(公告)日：2004-04-22
申请号：US10718628
申请日：2003-11-24
申请人： RIKEN
发明人： Sigeyuki Yokoyama , Jun Hoseki , Takato Yano , Yoshinori Koyama , Seiki Kuramitsu , Hiroyuki Kagamiyama
IPC分类号： C12Q001/68 , C07H021/04 , C12N009/10 , C12N001/21 , C12P021/02 , C12N015/74
CPC分类号： C12N9/1241 , C12N2310/111
摘要： To obtain a selective marker suitable for screening of thermophilic bacteria such as Thermus thermophilus. T. thermophilus are good research materials for investigating the interrelation between enzyme structures and functions since they are stable at extreme pH, crystallize easily and are easy-to-handle. To provide a novel kanamycin nucleotidyltransferase with markedly improved thermostability, a selective marker using the same, and, a screening method for thermophilic bacteria such as Thermus thermophilus using said selective marker.
摘要翻译：获得适合筛选嗜热菌嗜热菌嗜热菌的选择标记。嗜热链球菌是调查酶结构和功能之间相互关系的好材料，因为它们在极端pH下稳定，易于结晶并易于处理。提供具有显着改善的热稳定性的新型卡那霉素核苷酸转移酶，使用其的选择性标记，以及使用所述选择性标记的嗜热细菌例如嗜热栖热菌的筛选方法。

62. 发明申请

US20040072308A1 Process for producing d-serine 失效
标题翻译：生产D-丝氨酸的方法
公开(公告)号：US20040072308A1
公开(公告)日：2004-04-15
申请号：US10415107
申请日：2003-09-03
发明人： Hajime Ikeda , Yoshiyuki Yonetani , Shin-ichi Hashimoto
IPC分类号： C12P013/06 , C12N001/21
CPC分类号： C12R1/19 , C12P13/06 , C12P41/001
摘要： The present invention relates to: a process for producing D-serine wherein a microbial cell which is modified to have a higher L-serine deaminase activity than Escherichia coli DH5null strain, a culture of said cell, or a processed product thereof is brought into contact with DL-serine in a DL-serine-containing medium to decompose L-serine, and the remaining D-serine is recovered from the medium; and a microorganism used for this production process. D-serine is a useful compound as a synthetic intermediate for useful medicaments such as D-cycloserine.
摘要翻译：本发明涉及：生产D-丝氨酸的方法，其中将经修饰以具有比大肠杆菌DH5α菌株更高的L-丝氨酸脱氨酶活性，所述细胞培养物或其加工产物的微生物细胞接触在DL-丝氨酸培养基中用DL-丝氨酸分解L-丝氨酸，从培养基中回收剩余的D-丝氨酸; 和用于该生产过程的微生物。 D-丝氨酸是有用的药物如D-环丝氨酸作为合成中间体的有用化合物。

63. 发明申请

US20040071731A1 Chimeric protein comprising non-toxic pseudomonas exotoxin a and type iv pilin sequences 失效
标题翻译：包含无毒假单胞菌外毒素a和iv型pilin序列的嵌合蛋白
公开(公告)号：US20040071731A1
公开(公告)日：2004-04-15
申请号：US10432412
申请日：2003-05-21
发明人： David J. Fitzgerald
IPC分类号： A61K039/02 , C07H021/04 , C07K014/195 , C12P021/02 , C12N001/21
CPC分类号： C07K14/21 , A61K39/00 , A61K2039/53 , C07K2319/00
摘要： The invention provides chimeric proteins comprising a non-toxic Pseudomonas exotoxin A sequence and a Type IV pilin loop sequence, wherein the Type IV loop sequence is inserted within the non-toxic Pseudomonas exotoxin A. The invention also provides polynucleotides encoding the chimeric proteins, and compositions comprising the polynucleotides or the chimeric proteins. The invention also provides methods for using the chimeric proteins, polynucleotides and compositions of the invention.
摘要翻译：本发明提供了包含无毒假单胞菌外毒素A序列和IV型胆碱环序列的嵌合蛋白，其中IV型环序列插入无毒假单胞菌外毒素A内。本发明还提供了编码嵌合蛋白的多核苷酸，以及包含多核苷酸或嵌合蛋白的组合物。本发明还提供了使用本发明的嵌合蛋白，多核苷酸和组合物的方法。

64. 发明申请

US20040067561A1 Process for the production of L-lysine using coryneform bacteria 审中-公开
标题翻译：使用棒状细菌生产L-赖氨酸的方法
公开(公告)号：US20040067561A1
公开(公告)日：2004-04-08
申请号：US10629551
申请日：2003-07-30
申请人： Degussa AG
发明人： Brigitte Bathe , Caroline Reynen , Walter Pfefferle
IPC分类号： C12P013/08 , C12N001/21
CPC分类号： C12P13/08 , A23K10/12 , A23K20/142
摘要： A process for the production of L-lysine, comprising: a) fermentation of the L-lysine producing coryneform bacteria that are at least sensitive to diaminopimelic acid analogues, in particular 4-hydroxydiaminopimelic acid; b) enrichment of the L-lysine in the medium or in the bacterial cells; and optionally c) isolation of the L-lysine or L-lysine-containing feedstuffs additive from the fermentation broth, so that null0 to 100% of the constituents from the fermentation broth and/or from the biomass are present, and optionally bacteria are used in which in addition further genes of the biosynthesis pathway of L-lysine are enhanced, or bacteria are used in which the metabolic pathways that reduce the formation of L-lysine are at least partially switched off.
摘要翻译：一种生产L-赖氨酸的方法，包括：a）至少对二氨基庚二酸类似物，特别是4-羟基二氨基庚二酸敏感的产生L-赖氨酸的棒状细菌的发酵; b）在培养基或细菌细胞中富集L-赖氨酸; 并且任选地c）从发酵液中分离含有L-赖氨酸或L-赖氨酸的饲料添加剂，使得存在来自发酵液和/或来自生物质的组分的0至100％，以及任选的细菌其中还增加了L-赖氨酸的生物合成途径的其它基因，或者使用其中减少L-赖氨酸形成的代谢途径至少部分切断的细菌。

65. 发明申请

US20040067555A1 Recombinant hosts suitable for simultaneous saccharification and fermentation 失效
标题翻译：适合同时进行糖化和发酵的重组宿主
公开(公告)号：US20040067555A1
公开(公告)日：2004-04-08
申请号：US10377406
申请日：2003-02-27
申请人： University of Florida Research Foundation, Incorporated
发明人： Lonnie O. Ingram , Shengde Zhou
IPC分类号： C07H021/04 , C12P021/02 , C12N001/21 , C07K014/245
CPC分类号： C12N15/1051 , C12N9/2405 , C12N15/67 , C12N15/70 , C12P7/065 , C12P7/10 , Y02E50/16 , Y02E50/17 , Y02P20/582
摘要： The invention provides recombinant host cells containing at least one heterologous polynucleotide encoding a polysaccharase under the transcriptional control of a surrogate promoter capable of increasing the expression of the polysaccharase. In addition, the invention further provides such hosts with genes encoding secretory protein/s to facilitate the secretion of the expressed polysaccharase. Preferred hosts of the invention are ethanologenic and capable of carrying out simultaneous saccharification fermentation resulting in the production of ethanol from complex cellulose substrates.
摘要翻译：本发明提供了在能够增加多糖酶表达的替代启动子的转录控制下含有至少一种编码多糖酶的异源多核苷酸的重组宿主细胞。此外，本发明进一步提供了这样的宿主具有编码分泌蛋白的基因以促进表达的多糖酶的分泌。本发明的优选的宿主是具有酿酒酵母的并且能够进行同时糖化发酵，从而从复杂的纤维素底物生产乙醇。

66. 发明申请

US20040064857A1 Nuclear male sterile plants, methods of producing same and methods to restore fertility 审中-公开
标题翻译：核雄性不育植物，生产方法和恢复生育力的方法
公开(公告)号：US20040064857A1
公开(公告)日：2004-04-01
申请号：US10447029
申请日：2003-05-28
发明人： Cornelis Maria Petrus Van Dun , Oscar Johannes Maria Goddijn
IPC分类号： A01H001/00 , C12N015/82 , C12N001/21
CPC分类号： C12N15/8245 , C12N9/16 , C12N15/8289
摘要： The present invention is directed to the production of male sterile plants by providing them with a recombinant DNA capable of specific expression in the male reproductive system of a plant of the enzyme trehalose phosphate phosphatase (TPP). Restoration of the fertility can be established either by providing said male sterile plants with a recombinant DNA capable of expression of trehalose phosphate synthase (TPS) under control of an inducible promoter or with a recombinant DNA capable of expression of a suppressor protein which suppresses expression of TPP under control of an inducible promoter. This inducible restoration possibilities enable the maintenance site-specific recombination system is provided, by inserting two site-specific recombination sites flanking the recombinant DNA coding for TPP and crossing the male sterile lines with lines expressing the corresponding recombinase. By crossing the recombinase will excise the gene coding for TPP and fertile hybrids are produced.
摘要翻译：本发明涉及通过向其提供能够在磷酸海藻糖磷酸酶（TPP）的植物的雄性生殖系统中特异性表达的重组DNA来生产雄性不育植物。生育力的恢复可以通过在所述雄性不育植物提供能够在诱导型启动子控制下能够表达海藻糖磷酸合成酶（TPS）的重组DNA或能够表达抑制蛋白的表达的重组DNA来建立 TPP在诱导型启动子的控制下。通过插入编码TPP的重组DNA侧翼的两个位点特异性重组位点，并将雄性不育系与表达相应重组酶的系交叉，这种可诱导的修复可能性使得能够提供维持部位特异性重组系统。通过交叉重组酶将切除编码TPP的基因，并产生可育杂种。

67. 发明申请

US20040063139A1 Detection and identification of enteric bacteria 失效
公开(公告)号：US20040063139A1
公开(公告)日：2004-04-01
申请号：US10688108
申请日：2003-10-17
申请人： Kimberly-Clark Worldwide, Inc.
发明人： Stephen Quirk
IPC分类号： C12Q001/68 , C07H021/04 , C12N001/21
CPC分类号： C12Q1/689 , C12Q1/04 , C12Q1/10 , C12Q1/26 , Y02A50/451
摘要： The invention provides probes, antibodies and methods for detecting a gene that is only found in Enterobacteriaceae, the deoxyguanosine triphosphate triphosphohydrolase gene. These probes and methods are useful for the detecting whether test samples, including food and water samples, are infected with enteric bacteria.

68. 发明申请

US20040058448A1 PRODUCTION OF COMPLEX CARBOHYDRATES 有权
标题翻译：复合碳水化合物的生产
公开(公告)号：US20040058448A1
公开(公告)日：2004-03-25
申请号：US09991374
申请日：2001-11-16
发明人： Michael A. Apicella , Bradford W. Gibson , Nancy J. Phillips
IPC分类号： C12P019/28 , C12P019/04 , C08B037/00 , C12N015/74 , C12N001/21
CPC分类号： C12N9/1051 , C12P19/04 , C12P19/18
摘要： Compositions and methods for making complex carbohydrates in a bacterial production cell are disclosed. The complex carbohydrates that can be made include oligosaccharides and polysaccharides of bacterial or mammalian origin.
摘要翻译：公开了在细菌生产细胞中制备复合碳水化合物的组合物和方法。可以制备的复合碳水化合物包括细菌或哺乳动物来源的寡糖和多糖。

69. 发明申请

US20040058410A1 Fermentative carotenoid production 失效
公开(公告)号：US20040058410A1
公开(公告)日：2004-03-25
申请号：US10695980
申请日：2003-10-29
申请人： ROCHE VITAMINS, INC.
发明人： Luis Pasamontes , Yuri Tsygankov
IPC分类号： C12P023/00 , C12N009/10 , C12N001/21 , C12N015/74
CPC分类号： C12P23/00 , C12N15/52
摘要： Novel proteins of microorganism E-396 (FERM BP-4283) and the DNA sequences which encode these proteins have been discovered to provide an improved biosynthetic pathway from farnesyl pyrophosphate and isopentyl pyrophosphate to various carotenoids, especially zeaxanthin, astaxanthin, adonixanthin and canthaxanthin.

70. 发明申请

US20040058361A1 Antibacterial treatments 审中-公开
标题翻译：抗菌治疗
公开(公告)号：US20040058361A1
公开(公告)日：2004-03-25
申请号：US10451510
申请日：2003-06-20
发明人： Sutherland Kester Maciver
IPC分类号： C12Q001/68 , G01N033/554 , G01N033/569 , C12N001/21 , C07K016/12 , C12N001/10
CPC分类号： C07K14/44
摘要： The present invention provides a method of providing a bacterium binding component suitable for use in the preparation of a product for use in combating a target bacterium (including the inactivation of said bacterium and/or the treatment or diagnosis of an infection by said bacterium). The method comprises the steps of: contacting bacterium binding components of an eukaryotic micro-organism with the target bacterium for binding of the target bacterium with a bacterium binding component, and lysing the eukaryotic micro-organism; separating out the bacterium; treating the separated out bacterium so as to release thes bacterium binding component form said bacterium; and recovering the bacterium binding components. The invention also provides therapeutic and diagnostic products incorporating bacterial binding components.
摘要翻译：本发明提供一种提供适用于制备用于对抗靶细菌（包括所述细菌的灭活和/或所述细菌感染的治疗或诊断）的产品的细菌结合成分的方法。该方法包括以下步骤：将真核微生物的细菌结合成分与目标细菌接触，使靶细菌与细菌结合成分结合，并使真核微生物裂解; 分离出细菌; 处理分离出的细菌以便释放出细菌的细菌结合成分; 并回收细菌结合成分。本发明还提供结合细菌结合成分的治疗和诊断产品。

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