专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

61. 发明申请

US20020151054A1 Cellular production control 审中-公开
标题翻译：蜂窝生产控制
公开(公告)号：US20020151054A1
公开(公告)日：2002-10-17
申请号：US10090849
申请日：2002-03-04
发明人： Peter David Rathjen , Joy Rathjen , Kathryn May Hudson
IPC分类号： C12N005/08
CPC分类号： C12N5/0618 , A61K35/12 , C12N2501/58 , C12N2502/14 , C12N2506/02 , C12N2517/00
摘要： A method for the preparation of cells of ectodermal or endodermal lineages, of high purity which method includes providing a source of pluripotent cells; a source of a mesodermal suppression composition including cellular fibronectin (cFN); and a suitable culture medium; and culturing the pluripotent cells in the culture medium in the presence of the mesodermal suppression composition for a time sufficient to permit differentiation to ectoderm cells.
摘要翻译：用于制备高纯度的外胚层或内胚层谱系的细胞的方法，包括提供多能细胞来源; 中胚层抑制组合物的来源包括细胞纤连蛋白（cFN）; 和合适的培养基; 并在中胚层抑制组合物的存在下培养培养基中的多能细胞足以允许分化为外胚层细胞的时间。

62. 发明授权

US5625127A Extended human hematopoiesis in a heterologous host 失效
标题翻译：在异源宿主中延长人类造血功能
公开(公告)号：US5625127A
公开(公告)日：1997-04-29
申请号：US434706
申请日：1995-05-04
申请人： Hideto Kaneshima , Reiko Namikawa , Joseph M. McCune
发明人： Hideto Kaneshima , Reiko Namikawa , Joseph M. McCune
IPC分类号： A01K67/027 , C12N15/85 , A61K35/00
CPC分类号： A01K67/0271 , C12N15/8509 , A01K2217/05 , A01K2267/025 , A01K2267/0381 , C12N2517/00
摘要： A human hematopoietic system is provided in an immunocompromised mammalian host, where the hematopoietic system is functional for extended periods of time. Particularly, human fetal liver tissue and human fetal thymus is introduced into an appropriate site of a young immunocompromised mouse at a site supplied with a vascular system, whereby the fetal tissue results in novel formation of functional human bone marrow tissue.
摘要翻译：在免疫受损的哺乳动物宿主中提供人类造血系统，其中造血系统在延长的时间段内起作用。特别地，在供应有血管系统的部位将人胎肝组织和人胎儿胸腺引入幼年免疫受损小鼠的适当部位，由此胎儿组织导致功能性人骨髓组织的新形成。

63. 发明公开

EP3217785A4 FUNCTIONAL SEX-REVERSAL OF DECAPOD CRUSTACEAN FEMALES 审中-公开
公开(公告)号：EP3217785A4
公开(公告)日：2018-06-20
申请号：EP15859875
申请日：2015-11-13
申请人： ENZOOTIC HOLDINGS LTD
发明人： SHECHTER ASSAF , ROSEN OHAD , SAGI AMIR
IPC分类号： A01K61/00 , A01K67/033 , C12N5/07 , C12N5/071 , C12N5/16 , C12N15/873
CPC分类号： C12N5/0601 , A01K67/033 , A01K2227/70 , A01K2267/02 , C12N5/0683 , C12N2500/02 , C12N2510/00 , C12N2517/00
摘要： The present invention provides a primary cell culture which combines a cell culture medium and cells derived from a hypertrophied androgenic gland (AG) of a decapod crustacean. The invention also provides methods for obtaining an all-female progeny by initially injecting/transplanting the primary cell culture to a genetic-female to obtain a male-Neo-male.

64. 发明公开

EP3161128A2 METHODS AND COMPOSITIONS FOR TARGETED GENETIC MODIFICATIONS AND METHODS OF USE 审中-公开
标题翻译：方法和组合物进行有针对性的遗传修饰和方法使用
公开(公告)号：EP3161128A2
公开(公告)日：2017-05-03
申请号：EP15745288.9
申请日：2015-06-26
申请人： Regeneron Pharmaceuticals, Inc.
发明人： FRENDEWEY, David , DROGUETT, Gustavo , GAGLIARDI, Anthony , KUNO, Junko , AUERBACH, Wojtek , VALENZUELA, David M.
IPC分类号： C12N5/0735
CPC分类号： C12N15/8509 , A01K67/0275 , A01K67/0276 , A01K67/0278 , A01K2217/075 , A01K2227/105 , A01K2267/02 , A01K2267/0393 , C12N5/0606 , C12N15/907 , C12N2500/60 , C12N2517/00
摘要： Methods and compositions are provided for generating targeted genetic modifications on the Y chromosome or a challenging target locus. Compositions include an in vitro culture comprising an XY pluripotent and/or totipotent animal cell (i.e., XY ES cells or XY iPS cells) having a modification that decreases the level and/or activity of an Sry protein; and, culturing these cells in a medium that promotes development of XY F0 fertile females. Such compositions find use in various methods for making a fertile female XY non-human mammal in an F0 generation.
摘要翻译：提供了用于在Y染色体或一个具有挑战性的靶基因座上产生有针对性的遗传修饰的方法和组合物。组合物包括在体外培养多能XY和/或全能动物细胞具有修饰确实降低的Sry基因蛋白的水平和/或活性（即，XY ES细胞或iPS细胞XY）的，包括：并且，在论文中培养细胞并促进XY F0育女性的发展。搜索组合物可用于在F0代能制备能育的雌性XY非人类的哺乳动物的多种方法使用。

65. 发明授权

EP1290139B1 METHODS FOR ASSAYING GENE IMPRINTING AND METHYLATED CpG ISLANDS 有权
标题翻译：试验方法Standard根印迹和甲基化CpG岛
公开(公告)号：EP1290139B1
公开(公告)日：2006-03-01
申请号：EP01941519.9
申请日：2001-05-22
申请人： THE JOHNS HOPKINS UNIVERSITY
发明人： FEINBERG, Andrew The Johns Hopkins University , STRICHMAN-ALMASHANU, Liora The Johns Hopkins Univ. , JIANG, Shan c/o The Johns Hopkins University
IPC分类号： C12N5/00
CPC分类号： C12N5/0611 , C12N2501/115 , C12N2501/125 , C12N2501/235 , C12N2501/385 , C12N2502/13 , C12N2503/00 , C12N2503/02 , C12N2510/00 , C12N2517/00 , C12Q1/6827 , C12Q1/6886 , C12Q2600/118 , C12Q2600/136 , C12Q2600/154
摘要： Genomic imprinting is a parent of origin-dependent gene silencing that involves marking of alleles in the germline and differential expression in somatic cells of the offspring. Imprinted genes and abnormal imprinting have been implicated in development, human disease, and embryonic stem cell transplantation. We have established a model system for genomic imprinting using pluripotent 8.5 d.p.c. mouse embryonic germ (EG) cell lines derived from an interspecific cross. We find that allele-specific imprinted gene expression has been lost in these cells. However, partial restoration of allele-specific silencing can occur for some imprinted genes after in vitro differentiation of EG cells into somatic cell lineages, indicating the presence of a gametic memory that is separable from allele-specific gene silencing. We have also generated a library containing most methylated CpG islands. A subset of these clones was analyzed and revealed a subdivision of methylated CpG islands into 4 distinct subtypes: CpG islands belonging to high copy number repeat families; unique CpG islands methylated in all tissues; unique methylated CpG islands that are unmethylated in the paternal germline; and unique CpG islands methylated in tumors. This approach identifies a methylome of methylated CpG islands throughout the genome.

66. 发明公开

EP1616001A1 METHOD FOR IMPROVING GERMLINE TRANSMISSION EFFICIENCY OF AVIAN PRIMORDIAL GERM CELLS 审中-公开
标题翻译：一种用于改善原始生殖BIRD电池的效率种系传递
公开(公告)号：EP1616001A1
公开(公告)日：2006-01-18
申请号：EP04728713.1
申请日：2004-04-21
申请人： Seoul National University Industry Foundation , Hanmi Pharm. Co., Ltd. , AVICORE BIOTECHNOLOGY INSTITUTE, INC.
发明人： HAN, Jae Yong , PARK, Tae Sub , HONG, Yeong Ho 105-910 Woncheon Jugong , KWON, Se Chang
IPC分类号： C12N5/06 , C12N5/10
CPC分类号： C12N15/873 , C12N5/0611 , C12N2500/44 , C12N2501/105 , C12N2501/115 , C12N2501/125 , C12N2501/23 , C12N2501/235 , C12N2517/00
摘要： The present invention relates to a method for improving the germline transmission efficiency of avian primordial germ cells (PGCs), and methods for producing avian chimeras and transgenic using it. The present method comprises the steps of (a) isolating primordial germ cells (PGCs) from an avian embryonic gonad; and (b) culturing said PGCs in vitro for at least 5 days. According to the present method, the germline transmission efficiency of PGCs can be dramatically improved in a feasible manner.

67. 发明公开

EP0322240A2 Chimeric immunocompromised mammals and their use 无效
标题翻译： SchimäreimmunkompromittierendeSäugetiereund ihre Verwendung。
公开(公告)号：EP0322240A2
公开(公告)日：1989-06-28
申请号：EP88312222.8
申请日：1988-12-22
申请人： THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
发明人： McCune,Joseph,McCrary
IPC分类号： C12N15/00 , C12P21/00
CPC分类号： G01N33/56977 , A01K67/0271 , C07K16/00 , C07K16/18 , C12N2517/00 , G01N33/56972 , G01N33/56988
摘要： Chimeric hosts are provided by introducing into an immunocompromised mammalian host xenogeneic, usually human, tissue under conditions where the xenogeneic tissue may both grow and interact, in such a way that undifferentiated ("stem") cells may be observed to differentiate and to proliferate. For example, tissues from hematopoietic organs are employed where tissue from different organs may be used which allow for differentiation and proliferation of the xenogeneic hematopoietic stem cells. Into SCID mice may be introduced fetal tissue, fetal liver tissue providing stem cells, thymus tissue, where the thymus grows into a competent thymus organ, and lymph node tissue, where the lymph node tissue grows into a competent lymph node, and the stem cells are processed to produce functional human B- and T-cells.
摘要翻译：嵌合宿主通过在异种组织可能生长和相互作用的条件下引入免疫受损的哺乳动物宿主异种，通常为人类组织来提供，使得可观察到未分化（“茎”）细胞分化和增殖。例如，使用来自造血器官的组织，其中可以使用来自不同器官的组织，其允许异种造血干细胞的分化和增殖。可以将SCID小鼠引入胎儿组织，提供干细胞的胎儿肝组织，胸腺组织，其中胸腺生长成有效的胸腺器官和淋巴结组织，其中淋巴结组织生长成感受态淋巴结，并且干细胞被处理以产生功能性人B细胞和T细胞。

68. 发明申请

WO2014171824A1 BLASTOID, CELL LINE BASED ARTIFICIAL BLASTOCYST 审中-公开
标题翻译： BLASTOID，细胞系的人造芽孢杆菌
公开(公告)号：WO2014171824A1
公开(公告)日：2014-10-23
申请号：PCT/NL2014/050239
申请日：2014-04-16
申请人： UNIVERSITEIT TWENTE , KONINKLIJKE NEDERLANDSE AKADEMIE VAN WETENSCHAPPEN
发明人： RIVRON, Nicolas Clément , VAN BLITTERSWIJK, Clemens Antoni , GEIJSEN, Niels , VRIJ, Erik Jacob
IPC分类号： C12N5/073 , C12N5/0735 , A01K67/027
CPC分类号： C12N5/0603 , A01K67/027 , A01K2227/105 , A01K2267/02 , A61B17/435 , C12N5/0604 , C12N5/0697 , C12N15/873 , C12N2500/44 , C12N2501/01 , C12N2501/105 , C12N2501/119 , C12N2501/15 , C12N2501/16 , C12N2501/235 , C12N2501/392 , C12N2501/415 , C12N2501/727 , C12N2501/91 , C12N2502/02 , C12N2502/025 , C12N2517/00 , C12N2531/00 , C12N2535/00
摘要： The invention relates to a method for making an at least double layered cell aggregate and/or an artificial blastocyst, and/or a further-developed blastoid termed blastoid, by forming a double layered cell aggregate from at least one trophoblast cell and at least one pluripotent and/or totipotent cell, and culturing said aggregate to obtain an artificial blastocyst. This artificial blastocyst has a trophectoderm-like tissue that surrounds a blastocoel and an inner cell mass-like tissue. The cell aggregate can be formed from toti- or pluripotent stem cell types, or induced pluripotent stem cell types, in combination with trophoblast stem cells. Formation of a blastoid can be achieved by culturing the cell aggregate in a medium preferably comprising one or more of a Rho/ROCK inhibitor, a Wnt pathway modulator, a PKA pathway modulator, a PKC pathway modulator, a MAPK pathway modulator, a STAT pathway modulator, an Akt pathway modulator, a Tgf pathway modulator and a Hippo pathway modulator. The invention further relates to a method for growing an at least double layered cell aggregate into an artificial blastocyst, and into a further-developed blastoid, a fetus or a live animal. The invention further pertains to an in vitro cell culture comprising the mentioned compounds and/or cell aggregates.
摘要翻译：本发明涉及一种通过从至少一个滋养层细胞和至少一个滋养层细胞形成双层细胞聚集体来制备至少双层细胞聚集体和/或人造囊胚和/或进一步开发的称为“ 多能和/或全能细胞，并培养所述骨料以获得人造囊胚。这种人造囊胚具有围绕胚泡和内细胞块状组织的滋养外胚层样组织。细胞聚集体可以由干细胞或多能干细胞类型或诱导多能干细胞类型与滋养层干细胞组合形成。可以通过在优选包含Rho / ROCK抑制剂，Wnt途径调节剂，PKA途径调节剂，PKC途径调节剂，MAPK途径调节剂，STAT途径中的一种或多种的培养基中培养细胞聚集体来实现blastoid的形成调节剂，Akt途径调节剂，Tgf途径调节剂和河马途径调节剂。本发明还涉及一种将至少双层细胞聚集体生长成人造囊胚并进一步发育的胚轴，胎儿或活动物的方法。本发明还涉及包含所述化合物和/或细胞聚集体的体外细胞培养物。

69. 发明申请

WO2008106701A2 DNA SEGREGATION 审中-公开
标题翻译：脱氧核糖核酸
公开(公告)号：WO2008106701A2
公开(公告)日：2008-09-12
申请号：PCT/AT2008000071
申请日：2008-03-03
申请人： VETERINAERMEDIZINISCHE UNI WIE , STEINBORN RALF , DINNYES ANDRAS
发明人： STEINBORN RALF , DINNYES ANDRAS
IPC分类号： C12N15/877
CPC分类号： C12N15/877 , C12N2517/00
摘要： The present invention relates to a method for producing in vitro a homo- or heteroplasmic cell or cell population from a donor and a recipient cell: providing an enucleated recipient cell comprising mitochondrial DNA (mtDNA), fusing said recipient cell with a donor cell or introducing a nucleus of a donor cell into the recipient cell, and cultivating the fused recipient cell, characterized in that the mitochondrial DNA (mtDNA) of the recipient cell is at least in part mildly deleterious.
摘要翻译：本发明涉及用于从供体和受体细胞体外产生同质或异质细胞或细胞群的方法：提供包含线粒体DNA（mtDNA）的去核受体细胞，将所述受体细胞与供体细胞融合或引入供体细胞的核进入受体细胞，并培养融合的受体细胞，其特征在于受体细胞的线粒体DNA（mtDNA）至少部分是轻度有害的。

70. 发明申请

WO0190313A9 METHODS FOR ASSAYING GENE IMPRINTING AND METHYLATED CpG ISLANDS 审中-公开
标题翻译：用于测定基因印迹和甲基化CpG岛的方法
公开(公告)号：WO0190313A9
公开(公告)日：2003-03-06
申请号：PCT/US0116253
申请日：2001-05-22
申请人： UNIV JOHNS HOPKINS , FEINBERG ANDREW , STRICHMAN-ALMASHANU LIORA , JIANG SHAN
发明人： FEINBERG ANDREW , STRICHMAN-ALMASHANU LIORA , JIANG SHAN
IPC分类号： A01K67/027 , A61P35/00 , C12N5/074 , C12N5/10 , C12N15/09 , C12Q1/02 , C12Q1/68 , C12N5/06 , C12N15/11 , G01N33/50
CPC分类号： C12N5/0611 , C12N2501/115 , C12N2501/125 , C12N2501/235 , C12N2501/385 , C12N2502/13 , C12N2503/00 , C12N2503/02 , C12N2510/00 , C12N2517/00 , C12Q1/6827 , C12Q1/6886 , C12Q2600/118 , C12Q2600/136 , C12Q2600/154
摘要： Genomic imprinting is a parent of origin-dependent gene silencing that involves marking of alleles in the germline and differential expression in somatic cells of the offspring. Imprinted genes and abnormal imprinting have been implicated in development, human disease, and embryonic stem cell transplantation. We have established a model system for genomic imprinting using pluripotent 8.5 d.p.c. mouse embryonic germ (EG) cell lines derived from an interspecific cross. We find that allele-specific imprinted gene expression has been lost in these cells. However, partial restoration of allele-specific silencing can occur for some imprinted genes after in vitro differentiation of EG cells into somatic cell lineages, indicating the presence of a gametic memory that is separable from allele-specific gene silencing. We have also generated a library containing most methylated CpG islands. A subset of these clones was analyzed and revealed a subdivision of methylated CpG islands into 4 distinct subtypes: CpG islands belonging to high copy number repeat families; unique CpG islands methylated in all tissues; unique methylated CpG islands that are unmethylated in the paternal germline; and unique CpG islands methylated in tumors. This approach identifies a methylome of methylated CpG islands throughout the genome.
摘要翻译：基因印记是起源依赖基因沉默的亲本，其涉及在种系中标记等位基因和在后代的体细胞中的差异表达。印迹基因和异常印记涉及发育，人类疾病和胚胎干细胞移植。我们已经建立了使用多能8.5 d.p.c的基因组印迹模型系统。来自种间交叉的小鼠胚胎胚芽（EG）细胞系。我们发现在这些细胞中已经丢失了等位基因特异性印记的基因表达。然而，在将EG细胞体外分化成体细胞谱系后，一些印迹基因可能发生等位基因特异性沉默的部分恢复，表明存在可与等位基因特异性基因沉默分离的配子记忆。我们还生成了一个含有大多数甲基化CpG岛的文库。分析了这些克隆的一个子集，并揭示了甲基化CpG岛分为4个不同的亚型：属于高拷贝数重复家族的CpG岛; 所有组织中独特的CpG岛甲基化; 在父系种系中未甲基化的独特的甲基化CpG岛; 和独特的CpG岛在肿瘤中甲基化。该方法鉴定了整个基因组中甲基化CpG岛的甲基化。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式